• Bulletin of the Korean Chemical Society
• /
• v.31 no.10
• /
• pp.2771-2775
• /
• 2010

We present a rapid biogenic method for the production of nanoscale gold particles using pear extract. The formation and stability of pear-derived gold nanoparticles (Pear-AuNPs) were monitored by ultraviolet-visible spectroscopy. Their morphology, elemental composition and crystalline phase were determined by transmission electron microscopy, energy-dispersive X-ray spectroscopy and selected area electron diffraction. The average core size of crystalline Pear-AuNPs was in the range of $10{\pm}5\;nm$ and the observed morphology was spherical. The X-ray photoelectron spectrum showed a strong peak for the pure 'Au' phase. The circular dichroism spectrum indicated the natural capping ability of the pear extract, which generated peptide-gold nanoparticles. These nanoparticles were stable in aqueous solution for two months. A cell viability assay of Pear-AuNPs showed biocompatibility with human embryonic kidney 293 cells. Accordingly, this eco-friendly process for the bio-mimetic production of Pear-AuNPs is nontoxic in nature; consequently, it will find potential application in nano-biotechnology.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.33 no.5
• /
• pp.455-463
• /
• 2007

The present study was designed to evaluate effects of the commonly used NSAIDs(acetaminophen, aspirin, and ibuprofen) on human periodontal ligament cells. Human periodontal ligament cells were grown from a cell line provided by Kyungpook National University. Effects of NSAIDs on the proliferation of human periodontal ligament cells were assessed using MTT assays. And then $PGE_2$ concentrations were determined by ELISA and the changes of cellular configuration were found by electron micrograph. The results were as follows; 1. The MTT assay demonstrated that the commonly used NSAIDs(acetaminophen, aspirin, and ibuprofen) had not significant cytotoxic effect on human periodontal ligament cells. 2. NSAIDs inhibited the $PGE_2$ synthesis of human periodontal ligament cells compared with the control group. These inhibitory effects had no significant differences with NSAID type and concentration. 3. Electron micrographs of human periodontal ligament cells treated with NSAIDs showed more narrow and irregular shape.

• Macromolecular Research
• /
• v.16 no.3
• /
• pp.253-260
• /
• 2008

Environmentally friendly biocomposites were made using plant-based natural fibers, such as henequen and kenaf. The natural fiber reinforced polypropylene (PP) and unsaturated polyester (UP) biocomposites were examined in terms of the reinforcing effect of natural fibers on thermoplastic and thermosetting polymers. Kenaf (KE) and henequen (HQ) fibers were treated with an electron beam (EB) of 10 and 200 kGy doses, respectively, or with a 5 wt% NaOH solution. Four types of biocomposites (KE/PP, HQ/PP, KE/UP and HQ/UP) were fabricated by compression molding and each biocomposite was characterized by dynamic mechanical analysis and thermogravimetric analysis. The kenaf fiber had the larger reinforcing effect on the dynamic mechanical properties of both PP and UP biocomposites than the henequen fiber. The highest storage modulus was obtained from the biocomposite with the combination of UP matrix and 200 kGy EB treated kenaf fibers.

• Asian-Australasian Journal of Animal Sciences
• /
• v.33 no.6
• /
• pp.992-1001
• /
• 2020

Objective: This study was conducted to investigate the effects of hot-melt extruded ZnO nano-particles (HME-ZnO) as an alternative for P-ZnO on growth performance, nutrient digestibility, Zn bioavailability, intestinal microbiota, and intestinal morphology of weanling pigs. Methods: A total of 450 piglets (Landrace×Yorkshire×Duroc) were randomly allotted to five treatments based on initial body weight and sex. The experimental diets were fed in a meal form as phase 1 from d 0 to 14 and phase 2 from d 15 to 28. Treatments were the control diet without ZnO supplementation, the diet containing 2,500 ppm Zn as ZnO, and three diets containing 500, 1,000, or 2,500 ppm Zn as HME-ZnO. Results: The overall result showed a higher (p<0.01) average daily gain in weanling pigs fed ZnO-supplemented diets in comparison to the control diet. There was a decrease (p<0.01) in fecal score in the ZnO-supplemented diets. Dietary supplementation of ZnO improved (p<0.05) crude protein digestibility. The weanling pigs fed the P-ZnO diet had a lower (p<0.01) Zn digestibility in the feces than HME-ZnO supplemented treatments. Weanling pigs fed diets supplemented with ZnO had greater (p<0.05) Lactobacillus spp. populations and lower Clostridium spp. (p<0.05) and Coliforms (p<0.01) populations in the ileum. Weanling pigs fed diets supplemented with increasing concentrations of HME-ZnO linearly decreased Clostridium spp. (p<0.05) and Coliforms (p<0.01) in the ileum. Lower (p<0.05) Clostridium spp. and Coliforms counts in the colon were observed in pigs fed with ZnO-supplemented diets. Weanling pigs fed diets supplemented with ZnO showed a greater (p<0.01) villus height in the duodenum. Conclusion: Dietary supplementation of HME-ZnO and P-ZnO showed a potential to improve the digestibility of protein, intestinal Coliform and Clostridium, villus height in duodenum and ileum. Moreover, HME-ZnO showed a higher Zn digestibility compared with P-ZnO.

• Journal of Bio-Environment Control
• /
• v.25 no.4
• /
• pp.302-307
• /
• 2016

The aim of this study was to examine the optimum wattage and installation height using nano-carbon fiber infrared heating lamp (NCFIHL) for heating energy saving and plug seedling production in plug seedling production greenhouse in winter season. NCFIHL of 700 and 900 W was installed over the bed ($1.2{\times}2.4m$) as 0.7, 1.0, and 1.3 m height, respectively, for the production of grafted watermelon seedling in venlo-type glasshouse. Watermelon (Citrullus lanatus (Thunb.) Manst.) 'Jijonggul' and gourd (Lagenaria leucantha Rusby.) 'Sunbongjang' were used as scions and rootstocks, respectively. The scions and rootstocks were grafted by single cotyledon ordinary splice grafting. Light intensity of NCFIHL was below the $1{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ in all treatment. Spectral distributions of NCFIHL presented mostly infrared area. When outside air temperature was below $10^{\circ}C$, 700 and 900 W NCFIHL installed with 0.7 m height treatment and 900 W NCFIHL installed with 1.0 m height treatment maintained the setting air temperature ($20^{\circ}C$) at night. In the result of taking thermal imaging, the grafted watermelons were getting warm fast in 900 W NCFIHL installed with 0.7 m height treatment at night. Compactness of the grafted watermelons was the greatest in 700 W NCFIHL installed with 1.3 m height treatment. The results indicate that NCFIHL installed above 1.0 m height using 700 W was suitable for production of plug seedling.

• Applied Chemistry for Engineering
• /
• v.21 no.5
• /
• pp.495-499
• /
• 2010

To develop chemical and biosensors, this paper studies sensing characteristics of bulk carbon nanotube (CNT) electrodes by means of their electrical impedance properties due to their large surface area and excellence chemical absorptivity. The sensors were fabricated in the form of film and nano web style by using composite process for mass production. The bulk composite electrodes were fabricated with singlewall and multi-wall carbon nanotubes based on host polymers such as Nafion and PAN, using a solution-casting and an electrospinning technique. The resistance and the capacitance of electrodes were measured with LCR meter under the various amounts of buffer solution to study the electrical impedance change properties of them. On the experimental of sensor electrode, impedance characteristics of the composite electrode are affected by its host polymer and nanofiller and its sensing response showed saturated result after applying some amounts of buffer solution for test chemical. Especially, the capacitance values showed drastic changes while the resistance values only changed within few percent range. It is deduced that the ions in the solution penetrated and diffused into the electrodes surface changed the electrical properties of the electrodes much like a doping effect.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.34 no.11
• /
• pp.1721-1725
• /
• 2010

This paper reports the fabrication of geometry-controlled carbon microneedles by a backside exposure method and pyrolysis. The SU-8 microneedles are a polymer precursor in a carbonization process, which geometries such as base diameter, spacing, and aspect ratio can be controlled in a photolithography step. Using this fabrication method, highly reproducible carbon microneedles, which have high aspect ratios of more than 10 and very sharp nanotips, can be realized. The quartz surface with carbon microneedles becomes very hydrophilic and its wettability is adjusted by carrying out the silane treatment. In the carbon microneedle array ($3\;{\mu}m{\times}3\;{\mu}m$), the contact angle is extremly enhanced (${\sim}180^{\circ}$); this will be advantageous in developing low-drag microfluidics and labs-on-a-chip as well as in other bio-applications.

• Journal of Bio-Environment Control
• /
• v.31 no.1
• /
• pp.8-13
• /
• 2022

This study was carried out to investigate the proper wattage and installation distance for the efficient use of nano-carbon fiber infrared heating lamp (NCFIHL), a heating device advantageous for heating energy saving, when the production of watermelon plug seedlings in the plug seedling nursery in winter season. Six small beds were divided into plastic film, and 700 W and 900 W nano-carbon fiber infrared heating lamps were installed at 100 cm above the bed. 1 lamp at central (control), 60 cm interval (2 lamps), and 40 cm interval (3 lamps) heating lamps were installed in each bed inside the greenhouse. All treatments, except the control, were set to keep the night air temperature at 20℃ after lighting the NCFIHL. The leaf temperature showed a tendency to increase fast as the install distance was narrow. The leaf length and leaf width tended to increase as the installation distance of the 700 W heating lamp was narrow. The compactness was high in 700 W heating lamp with 40 cm of installation distance. Therefore, in consideration of maintaining the set temperature at night, installing 700 W electric lamps at 40 cm was an efficient power and installation distance for watermelon grafted seedlings considering economic feasibility.

• JSTS:Journal of Semiconductor Technology and Science
• /
• v.5 no.1
• /
• pp.30-37
• /
• 2005

We present an air bridge type electrode system with tunable electrode distance for detecting electroactive biomolecules. It is known that the narrower gap between electrode fingers, the higher sensitivity in IDA (interdigitated array) electrode. In previous researches on IDA electrode, narrower patterning required much precise and expensive equipment as the gap goes down to nanometer scale. In this paper, an improved method is suggested to replace nano gap pattering with downsizing electrode distance and showed that the patterning can be replaced by thickness control using metal deposition methods, such as electroplating or metal sputtering. The air bridge type electrode was completed by the following procedures: gold patterning for lower electrode, copper electroplating, gold deposition for upper electrode, photoresist patterning for gold film support, and copper etching for space formation. The thickness of copper electroplating is the distance between upper and lower electrodes. Because the growth rate of electroplating is $0.5{\mu}m\;min^{-1}$, the distance is tunable up to hundreds of nanometers. Completed electrodes on the same wafer had $5{\mu}m$ electrode distance. The gaps between fingers are 10, 20, 30, and $40{\mu}m$ and the widths of fingers are 10, 20, 30, 40, and $50{\mu}m$. The air bridge type electrode system showed better sensitivity than planar electrode.

• Advances in nano research
• /
• v.9 no.2
• /
• pp.113-122
• /
• 2020

Surface-Enhanced Raman Scattering (SERS) spectroscopy is a multifaceted surface sensitive methodology which exploits spectroscopy-based analysis for various applications. This technique is based on the massive amplification of Raman signals which were feeble previously in order to use them for appropriate identification at qualitative and quantitative in chemical as well as biological systems. This novel powerful technique can be utilized to identify pathogens such as bacteria and viruses. As far as SERS is concerned, one of the most studied problems has been functionalization of SERS active substrate. Metal colloids and nanostructures or microstructures synthesized using noble metals such as Au, Ag and Cu are considered to be SERS active. Silver and gold are extensively used as SERS active substrates due to chemical inertness and stability in air compare to copper. However, use of Cu as a suitable alternative has been taken into account as it is cheap. Herein, we have synthesized air-stable copper microstructures/nanostructures by chemical, electrochemical and microwave-assisted methods. In this paper, we have also discussed the use of as synthesized copper micro/nanostructures as inexpensive yet effective SERS active substrates for the fast identification of micro-organisms like Staphylococcus aureus and Escherichia coli.