• Journal of Korean Society of Water and Wastewater
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• v.32 no.6
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• pp.487-497
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• 2018

Water quality improvement processes for stagnant area consist mainly of technologies applying vegetation and artificial water circulation, and these existing technologies have some limits to handle pollution loads effectively. To improve the purification efficiency, eco-friendly technologies should be developed that can reinforce self-purification functions. In this study, a multi-functional floating island combined with physical chemical biological functions ((1) flotation and oxidization using microbubbles, (2) vegetation purification and (3) bio-filtration with improved adsorption capacity) has been developed and basic experiments were performed to determine the optimal combination conditions for each unit process. It has been shown that it is desirable to operate the microbubble unit process under conditions greater than $3.5kgf/cm^2$. In vegetation purification unit process, Yellow Iris (Iris pseudacorus) was suggested to be suitable considering water quality, landscape improvement and maintenance. When granular red-mud was applied to the bio-filtration unit process, it was found that T-P removal efficiency was good and its value was also stable for various linear velocity conditions. The appropriate thickness of filter media was suggested between 30 and 45 cm. In this study, the optimal design and operating parameters of the multi-functional floating island have been presented based on the results of the basic experiments of each unit process.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.141-142
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• 2003

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.255.3-256
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• 2003

Marine Algae of order Chordariales are rich resources of bioactive metabolites. Methanolic extracts of the brown alga /shige Okamurae exhibited potent antioxidative and butyrylcholinesterase(BChE) inhibitory effects. Bio-guided purification [solvent partition, ODS flash, silica flash, gel-filtration on Sephadex LH 20, ODS HPLC] of them gave a compound 1. Its structure was elucidated by detailed analysis of spectroscopic data of 1 and comparison of literature data. A variety of bioassay for 1 is in progress.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.3
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• pp.99-110
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• 2018

Compared to yellow dust coming from China or particulate matter created naturally in spring due to Total Suspended Particulate(TSP), particulate matter in winter season have much more serious effect on human body as they penetrate cell membranes. Although such particulate matter are becoming a social issue, there are no concrete plans on how to reduce them. Air-purifying plants are limited in maintaining the indoor air quality of large area because it is usually difficult to quantify their performance. In order to improve this, a bio-filter that can be connected to air conditioner is suggested as an option. This study seeks to improve air conditioning model-based monitoring method for bio-filters from prior studies and objectify correlations between applied vegetation and growing environment into quantitative indicators. By doing so, this study seeks to provide criteria on plants applied to artificial soil-based vegetation bio-filters and basic information to set air-conditioning features. The study results confirmed significant tendency on the growing stability of each purifying plant in mechanical air-conditioning environment. Among three models selected for bio-filter vegetation models, epipremnum aureum showed high performance in quantitative indicators, including soil moisture, EC, and leaf temperature, etc., indicating that it would assure the highest growing stability in this test air-conditioning environment.

• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.852-858
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• 2008

An extracellular protease (Mc1) was isolated from the nematode-trapping fungus Monacrosporium cystosporium by gel filtration, anion-exchange, and hydrophobic interaction chromatographies. This protease had a molecular mass of approximately 38 kDa and displayed an optimal activity at pH 7-9 and $56^{\circ}C$ (over 30 min). Its proteolytic activity was highly sensitive to the serine protease inhibitor PMSF (phenylmethylsulfonylfluoride, 0.1 mM), indicating that it belonged to the serine-type peptidase group. The Michaelis constant ($K_m$) and $V_max$ for substrate N-Suc-Ala-Ala-Pro-Phe-pNA were $1.67{\times}10^{-4}\;M$ and 0.6071 $OD_{410}$ per 30 s, respectively. This protease could degrade a broad range of substrates including casein, gelatin, BSA (bovine serum albumin), and nematode cuticle. Moreover, the enzyme could immobilize the free-living nematode Panagrellus redivivus and the pine wood nematode Bursaphelenchus xylophilus, suggesting that it might playa role in infection against nematodes. The encoding gene of Mc1 was composed of one intron and two exons, coding for a polypeptide of 405 amino acid residues. The deduced amino acid sequence of Mcl showed 61.4-91.9% identity to serine proteases from other nematode-trapping fungi. Our results identified that Mcl possessed biochemical properties including optimal reaction condition and substrate preference that are different from previously identified serine proteases.

• Applied Biological Chemistry
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• v.51 no.4
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• pp.299-304
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• 2008

A ${\beta}$-1,4-endoglucanase gene from Bacillus subtilis H12 was cloned into Escherichia coli JM109 (pBC8) and sequenced. The endoglucanase gene with an insert DNA of 2.5 kb possessed an open reading frame of 1,500 bp encoding a mature protein of 499 amino acids with a calculated molecular mass of 55 kDa. The deduced amino acid sequence showed similarity to those of the known neutral cellulase genes of B. subtilis PAP115 (99.2%) and BSE616 (97.8%), as well as the alkaline gene of Bacillus sp. N4 (55.1%). The endoglucanase activity expressed by E. coli (pBC8) was localized in the periplasmic fraction (80%) and the cytoplasmic fraction (20%). An endoglucanase was purified from the periplasmic fraction by performing gel filtration and anion exchange chromatography. The molecular weight of the purified enzyme was estimated to be 31 kDa by SDS-PAGE, and the maximum activity occurred at pH 7 and $40^{\circ}C$. The enzyme easily hydrolyzed soluble substrates such as carboxymethyl cellulose and barely ${\beta}$-glucan, whereas the sigmacell and xylan, the known insoluble substrates, were not entirely hydrolyzed.

• Journal of Korean Society on Water Environment
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• v.22 no.1
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• pp.45-50
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• 2006

This study was performed to evaluate the air lift reactors (ALR) by variations of HRT and recycling rate. Air lift reactor was composed of bioreactor and clarifier above it. To remove organic matters and nitrogen through the formation of microbic film and filtration, bio-filter reactors were filled with clay, glass, bead, waste plastic, respectively. Influent wastewater was fed to biofilter reactor, and effluent wastewater from bio-filter reactor was injected ALR again, instead of adding external carbon source. Effluent BOD concentration was satisfied with lower than 10 mg/L in recycling rate 100% regardless of the variation of HRT and the kinds of media materials. In HRT 4 hr, recycling rate 100%, BOD removal efficiency rate was from about 85 to 90%, COD removal efficiency rate was higher than 90%. Effluent TN concentration was satisfied with less than 20 mg/L, if HRT was maintained by over than 6 hr regardless of recycling rate and media materials. Over than HRT was 4 hr, microbes concentration in air lift reactor was maintained over than 2,500 mg/L constantly, not sensitive to environmental condition, and organic removal was effective as it was higher.

• Journal of Korean Society on Water Environment
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• v.27 no.1
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• pp.44-53
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• 2011

Membrane bio-reactor (MBR) has several advantages over the conventional activated sludge process, including a high biomass, low sludge production, and better permeate quality. Therefore, the MBR have gained popularity for municipal and industrial wastewater treatment. However the MBR usually were used for sewage and low streng th wastewater treatment because of membrane fouling problem and limitation of oxygen transfer into biomass. In this study, the hybrid process combining MBR and pure oxygen was tested for high strength organic wastewater treatment in the COD loading range from 2 to $10kgCOD/m^3{\cdot}day$. The hybrid process, membrane coupled pure oxygen high compact reactor (MPHCR), had been operated for one year and operation parameters, the effect of COD loading, MLSS concentration and the location of membrane module were studied for membrane fouling characteristic. Also membrane resistance test and the component of foulant was analyzed to investigate what is specific foulant in the MBR.

• The Korean Journal of Food & Health Convergence
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• v.9 no.6
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• pp.9-19
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• 2023

Lactobacillus plantarum and Leuconostoc mesenteroides are crucial functional starters and predominant isolates in a wide range of fermented foods, particularly kimchi, whose constituents exhibit bioactive properties. We previously developed a methodology using anion exchange resins to purify peptidyl compounds from Lb. plantarum LBP-K10. Antibacterial cultures of Lb. plantarum LBP-K10 were obtained from the respective cultures' supernatants and filtrates. However, conclusive evidence of the efficacy of kimchi filtrates in eradicating pathogenic bacteria is lacking. We aimed to simulate the potential effects of antibacterial filtrates that contained antibacterial compounds which were derived from cultures of Lb. plantarum LBP-K10. We acquired the kimchi filtrates using a combination of centrifugation and filtration methodologies, without the requirement for inoculation. The filtered liquid from Chinese cabbage kimchi, inoculated with Lb. plantarum LBP-K10 as a starter culture, and the non-inoculated liquid from Chinese cabbage kimchi (referred to as CCK and CCKRef, respectively) were were examined. CCK demonstrated greater inhibitory activity and a more significant bactericidal effect against the bacterial indicator strains. The minimum inhibitory concentration demonstrated comparable outcomes in tests against both Gram-positive and Gram-negative bacteria. This research offers a groundbreaking examination that displays the effectiveness of profiling peptidyl compounds within kimchi filtrates for curing bacterial infections.

• Food Science of Animal Resources
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• v.22 no.4
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• pp.343-347
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• 2002

Bovine colostrum contains various bio-functional Proteins. Especially, transforming: growth factor-${\beta}$1 (TGF-${\beta}$1) has a function in concerns with immune response. The purpose of this study was to establish the purification Processing of transforming growth factor-${\beta}$1(TGF-${\beta}$1). The highest concentration of TGF-${\beta}$1 was measured within 48 h after parturition in bovine colostrum using ELISA kit. Purification of TGF-${\beta}$1 from whey protein was carried out by the gel filtration, AF-heparin chromatography and AF-heparin rechromatography. After final purification step, TGF-${\beta}$1 with a molecular weight of 25 kD was obtained, and confirmed by silver staining and western blotting. Finally, TGF-${\beta}$1 was identified native form of 25 kD and reducing form of 12.5 kD by reducing agent.