• 한국대기환경학회지
• /
• 제19권1호
• /
• pp.67-76
• /
• 2003

Recently consideration of health and interest on bio aerosols have been growing steadily. In this study, inertial impactor, which can be used to collect airborne particles and bio aerosols, was newly devised for real-time observation on the particles collected on impaction plate. and named Visual Impactor. Flow field and particle trajectory in the space between nozzle and impaction plate was analyzed numerically, and the collection effciencies were calculated. Calibration and performance evaluation of the Visual Impactor was conducted with polydisperse aerosols generated from 0.1％ sodium chloride solution. Cut-off diameter from numerical simulation was in good agreement with that from experimental results. Because of particle bounce and particle deposition on nozzle tip due to short jet-to-plate distance, the collection efficiencies from numerical and experimental analyses were different slightly. Visual Impactor was used to collect airborne particles, and the features of collected particles could be seen in real-time. Airborne particles in different weather conditions (fine, cloudy, and rainy) were sampled and compared one another The features of collected airborne particles were dependent strongly upon relative humidity. In addition, with hours elapsing, shapes and colors of collected particles were changed by evaporation and surface tension, etc.

• 한국균학회지
• /
• 제46권4호
• /
• pp.393-403
• /
• 2018

공기에 의해 운반되는 바이오에어러졸은 농산물 오염을 널리 일으키는 원인으로 알려져 있다. 육안으로 보이지 않는 진균 포자는 버섯 재배 중에 버섯과 인간의 건강에 해를 끼치는 바이오에어로졸이다. 본 연구는 한국의 표고버섯 재배사 실내의 공기 중에 존재하는 진균의 농도 및 종 분포에 관한 기초 자료를 얻기 위해 수행되었다. 2015년과 2016년에 국내 6 개의 다른 지역에 위치한 13 개의 표고버섯 재배 농장에서 21 차례 실내공기로부터 진균 포자를 샘플링하고 분석하였다. 공기 중 진균 농도는 $1.30{\times}10^2-1.59{\times}10^4cfu/m^3$ 범위였다. 중요하게도 20 차례의 농장방문 시료 채취 결과에서 진균 농도가 환경부의 실내공기질 권장 기준 농도인 $500cfu/m^3$를 초과하였다. 총 450 균주의 진균이 분리되었으며 46 속 33 종으로 동정되었다. 이중에 인체 위해균(4 속 4 종)과 식물병원균(10 속 13 종)이 존재하였다. 또한, 버섯 건강에 영향을 미치는 해로운 종인 Trichoderma harzianum, T. atroviride 및 T. longibrachiatum가 21 건 시료 채취 중 17 건에서 빈도 높게 검출되었다. 본 결과는 버섯생산의 재배관리를 위해서 재배사 실내 공기질을 개선해야 한다는 증거를 제시하였다.

• 한국대기환경학회:학술대회논문집
• /
• 한국대기환경학회 2008년도 춘계학술대회 논문집
• /
• pp.447-449
• /
• 2008

• 한국환경생태학회지
• /
• 제35권6호
• /
• pp.669-678
• /
• 2021

본 연구에서는 토지피복유형에 따른 바이오에어로졸 중 배양성 세균분포를 파악하기 위하여 충청북도 충주시에 위치한 3가지 유형을 대상으로 실시하였다. 토지피복에 따른 기상요소와 바이오에어로졸을 조사하여 측정지점별 미기상변화에 따른 세균 분포를 비교·분석할 수 있었다. 측정지점별 미기상 중 기온의 차이는 도시 숲 8.7℃, 수변 녹지 10.8℃, 도심지 10.2℃로 측정지점 중 도시 숲이 가장 낮은 기온 값을 보였으며, 상대습도는 도시 숲 61.8%, 수변 녹지 59.3%, 도심지 55.7%로 도시 숲이 가장 높은 것으로 분석되었다. 동정된 세균은 43속 99종으로 나타났다. 배양된 세균의 종 다양성 측면에서는 수변녹지 22속, 도시 숲 21속, 도심지 17속으로 조사되었으며, 종수의 경우 수변녹지 37종, 도심지 31종, 도시 숲 31종으로 나타났다. 3가지 유형의 측정지에 모두 존재하는 종은 Bacillus toyonensis와 Pseudarthrobacter oxydan으로 나타났으며, 또한 도시 숲과 수변녹지와 같은 녹지지역에서 존재하는 Herbiconiux flava종을 확인하였다. 측정지점별 미기상환경에 따른 세균농도의 변화를 분석한 결과 도시 숲 333CFU/m³, 수변 녹지 287CFU/m³, 도심지 173CFU/m³ 순으로 나타났으며, 미기상 중 기온변화에 따른 측정지점별 농도변화는 미비하였다. 그러나 상대습도와 풍속은 농도변화와 유사한 경향을 보인 것으로 분석되었다. 따라서 본 연구에서는 토지피복유형에 따른 바이오에어로졸 중 배양성 세균의 분포 정도를 파악하여 측정지점별 바이오에어로졸의 특성을 비교·분석하여 추후 건강한 도시 관리 및 녹지조성의 기초자료를 제공하고자 하였다.

• 실내환경 및 냄새 학회지
• /
• 제16권1호
• /
• pp.47-53
• /
• 2017

Mold is one of the important bio-aerosols affecting human health in the indoor environment. To manage mold contamination, it is necessary to use an appropriate method for its detection and enumeration. Recently, the impaction method of ISO 16000-18 has been established as one of methods to detect and enumerate molds in air. To investigate the general use of the impaction method for mold detection in domestic indoor environments, the suitability of the method was assessed using different antibiotics, media and air samplers. All of the three antibiotics tested - ampicillin, chloramphenicol and streptomycin - showed inhibitory effects on bacterial colony formation on MEA and DG-18 media, without inhibiting mold growth. Of these three antibiotics, ampicillin was the most effective. There was no statistical difference between MEA and DG-18 media in the measurement of mold concentration. The formation of discriminative colony morphology was more apparent in DG-18 media. No significant difference in the measurement of mold concentration was found between Andersen samplers and MAS-100NT samplers, which are two major samplers introduced in Korea.

• Safety and Health at Work
• /
• 제14권2호
• /
• pp.237-242
• /
• 2023

Background: This study evaluated occupational exposure levels of doxorubicin in healthcare workers performing rotational intraperitoneal pressurized aerosol chemotherapy (PIPAC) procedures. Methods: All samples were collected during PIPAC procedures applying doxorubicin to an experimental animal model (pigs). All procedures were applied to seven pigs, each for approximately 44 min. Surface samples (n = 51) were obtained from substances contaminating the PIPAC devices, surrounding objects, and protective equipment. Airborne samples were also collected around the operating table (n = 39). All samples were analyzed using ultra-high performance liquid chromatography-mass spectrometry. Results: Among the surface samples, doxorubicin was detected in only five samples (9.8%) that were directly exposed to antineoplastic drug aerosols in the abdominal cavity originating from PIPAC devices. The telescopes showed concentrations of 0.48-5.44 ng/cm² and the trocar showed 0.98 ng/cm² in the region where the spraying nozzles were inserted. The syringe line connector showed a maximum concentration of 181.07 ng/cm², following a leakage. Contamination was not detected on the surgeons' gloves or shoes. Objects surrounding the operating table, including tables, operating lights, entrance doors, and trocar holders, were found to be uncontaminated. All air samples collected at locations where healthcare workers performed procedures were found to be uncontaminated. Conclusions: Most air and surface samples were uncontaminated or showed very low doxorubicin concentrations during PIPAC procedures. However, there remains a potential for leakage, in which case dermal exposure may occur. Safety protocols related to leakage accidents, selection of appropriate protective equipment, and the use of disposable devices are necessary to prevent occupational exposure.

• 대한의생명과학회지
• /
• 제29권4호
• /
• pp.363-370
• /
• 2023

Human respiratory viral infections such as COVID-19 are highly contagious, so continuous management of airborne viruses is essential. In particular, indoor air monitoring is necessary because the risk of infection increases in poorly ventilated indoors. However, the current method of detecting airborne viruses requires a lot of time from sample collection to confirmation of results. In this study, we proposed a system that can monitor airborne viruses in real time to solve the deficiency of the present method. Air samples were collected in liquid form through a bio sampler, in which case the virus is present in low concentrations. To detect viruses from low-concentration samples, viral RNA was concentrated and extracted using silica-magnetic beads. RNA binds to silica under certain conditions, and by repeating this binding reaction, bulk samples collected from the air can be concentrated. After concentration and extraction, viral RNA is specifically detected through real-time qPCR (quantitative polymerase chain reaction). In addition, based on liquid handling technology, we have developed an automatic machine that automatically performs the entire testing process and can be easily used even by non-experts. To evaluate the system, we performed air sample collection and automated testing using bacteriophage MS2 as a model virus. As a result, the air-collected samples concentrated by 45 times then initial volume, and the detection sensitivity of PCR also confirmed a corresponding improvement.