• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.83-83
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• 2003

The lutropin/choriogonadotropin receptor (LHR) is a member of the rhodopsin-like subfamily of G protein coupled receptor (GPCRs), that has been shown to mediate the internalization of its two naturally occurring agonist, lutropin and choriogonadotropin (CG). The clustered agonist-receptor complex is internalized by a dynamin-dependent pathway and traverses the endosomal compartment without agonist dissociation Dissociation of the agonist-receptor complex occurs in the lysosomes, where both the agonist and receptor are degrade. Recently, constitutively activating mutations of the receptor have been identified that are associated with familial male-precocious puberty (FMPP). A FMPP is a form of sexual precocious puberty in boys in which testosterone levels are elevated independent of changes in luteinizing hormone-releasing hormone and serum luteinizing hormone levels, We have now analyzed two naturally occurring, constitutively active mutants of the human LHR. These mutations were introduced into the rat LHR (rLHR) and are designated L435R and D556Y. Cells expressing rLHR-D556Y bind human choriogonadotropin (hCG) with normal affinity, exhibit a 25-fold increase in basal cAMP and respond to hCG with a normal increase in cAMP accumulation. Cells expressing rLHR-L435R also bind hCG with normal affinity, exhibit a 47-fold increase in basal cAMP, and do not respond to hCG with a further increase in cAMP accumulation. This mutation enhances the internalization of the free and agonist-occupied receptors ~2- and ~17- fold, respectively We conclude that the state of activation of the rLHR can modulate its basal and/or agonist-stimulated internalization. Since the internalization of hCG is involved in the termination of hCG actions, we suggest that the lack of responsiveness detected in cells expressing rLHR-L435R is due to the fast rate of internalization of the bound hCG. The finding that membranes expressing rLHR-L435R respond to hCG with an increase in adenylyl cyclase activity supports this suggestion. Autonomous Leydig cell activity in FMPP is caused by a constitutively activating LH/CGR.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2003.10a
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• pp.319-322
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• 2003

Integrated coastal management (ICM) requires robust indicators that gauge the 'health' of the coast in relation to environmental, social and economic activities. Biological indicators(bio-indicators) offer a signal of the biological condition in an ecosystem. Using bio-indicators as an early warning of pollution or degradation in an ecosystem can help sustain critical resources. This review examines the rationale and value of selecting species as bio-indicators of human induced changes within estuaries, using examples from both the western and southern coast. It include a range of biological parameters relating to particular species, groups of species and biological processes. The use of these indicators is critically reviewed and the presence or absence of a relevant framework for their use in Korean ICM programs is discussed.

• Journal of Microbiology and Biotechnology
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• v.25 no.6
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• pp.845-855
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• 2015

The present study describes the gene cloning and high-level expression of an alkaline and thermostable lipase gene from Trichosporon coremiiforme V3. Nucleotide analysis revealed that this lipase gene has an open reading frame of 1,692 bp without any introns, encoding a protein of 563 amino acid residues. The lipase gene without its signal sequence was cloned into plasmid pPICZαA and overexpressed in Pichia pastoris X33. The maximum lipase activity of recombinant lipase was 5,000 U/ml, which was obtained in fed-batch cultivation after 168 h induction with methanol in a 50 L bioreactor. The purified lipase showed high temperature tolerance, and being stable at 60℃ and kept 45% enzyme activity after 1 h incubation at 70℃. The stability, effects of metal ions and other reagents were also determined. The chain length specificity of the recombinant lipase showed high activity toward triolein (C18:1) and tripalmitin (C16:0).

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.1173-1178
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• 2005

This paper proposes an IPMC actuating system with a bio-mimetic function. EMG signals generated by an intended contraction of muscles in forearm are used for the actuation of the IPMC. To obtain higher actuation force of the IPMC, the single layered as thick as 800 [${\mu}$m] or multi-layered IPMC (Nafion) of which each layer can be as thick as 178 [${\mu}$m] are prepared. The experimental results using an implemented IPMC control system show a possibility and a usability of the bio-mimetic artificial muscle.

• Journal of Power System Engineering
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• v.13 no.1
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• pp.65-71
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• 2009

To address the need for new intelligent sensing, this paper introduces nano sensors made of carbon nanotube (CNT) composites and presents their preliminary experiments. Having smart material properties such as piezoresistivity, chemical and bio selectivity, the nano composite can be used as smart electrodes of the nano sensors. The nano composite sensor can detect structural deterioration, chemical contamination and bio signal by means of its impedance measurement (resistance and capacitance). For a structural application, the change of impedance shows specific patterns depending on the structural deterioration and this characteristic is available for an in-situ multi-functional sensor, which can simultaneously detect multi symptoms of the structure. This study is anticipated to develop a new nano sensor detecting multiple symptoms in structural, chemical and bio applications with simple electric circuits.

• Bulletin of the Korean Chemical Society
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• v.35 no.10
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• pp.3047-3051
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• 2014

In this paper, we describe a new method for the selective extraction and quantification of glutathione (GSH) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and maleimide-presenting gold nanoparticles (Mal-AuNPs). Our strategy utilizes the Michael addition to selectively extract GSH, from chosen samples, onto the maleimide of Mal-AuNPs. After the extraction step, the GSH bound to the AuNPs was analyzed by MALDI-TOF MS in the presence of an internal standard which was prepared by reacting Mal-AuNPs with isotope-labeled GSH ($GSH^*$). The $GSH^*$ has the same structure as GSH but a higher molecular weight, and therefore, enables absolute quantification of GSH by comparing the mass signal intensities of the GSH- and $GSH^*$-conjugated alkanethiols. Our strategy was verified by analyzing GSH-spiked fetal bovine serum and NIH 3T3 cells.

• Proceedings of the IEEK Conference
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• 2002.07c
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• pp.1871-1874
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• 2002

This paper describes a realization of a linearized transconductor using a CMOS complementary pair. The proposed transconductor is driven by a grounded signal source. How to cancel the offset current is described, Moreover , how to control the transconductance is described . It is shown that power consumption of the proposed transconductor without the control circuit is about half as low as that of the conventional Wang’s OTA through computer simulation.

• Journal of Institute of Control, Robotics and Systems
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• v.13 no.12
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• pp.1186-1191
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• 2007

Human driver monitoring system is one of the most important systems for the safety in driving vehicles, and therefore driver assistance system has gained much attention during the last decade. This paper proposed an intelligent driver assistance system which monitors human driver's states from bio-signals such as ECG and Blood Pressure. The proposed system used mamdani fuzzy inference to evaluate the driver's mental strain and generated warning signals to the driver. The approach using bio-signals in driver assistance system is the main issue of the related systems and the preliminary results showed the possibility of further research topics in developing more intelligent embedded systems with bio-signal feedback.

• Applied Science and Convergence Technology
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• v.23 no.2
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• pp.61-71
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• 2014

A field-effect transistor (FET) is one of the most commonly used semiconductor devices. Recently, increasing interest has been given to FET-based biosensors owing totheir outstanding benefits, which are likely to include a greater signal-to-noise ratio (SNR), fast measurement capabilities, and compact or portable instrumentation. Thus far, a number of FET-based biosensors have been developed to study biomolecular interactions, which are the key drivers of biological responses in in vitro or in vivo systems. In this review, the detection principles and characteristics of FET devices are described. In addition, biological applications of FET-type biosensors and the Debye length limitation are discussed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.11
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• pp.5403-5408
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• 2012

In this paper, we introduce the multiple frequency bio-electrical impedance analysis method for body composition analysis. And then we implement the multiple frequency bio-electrical impedance analysis system. Overall system consists of: multiple frequency alternating current signal generator contained alternating current signal, phase signal detector, voltage signal detector, micro controller, in-out device(key-pad LCD), conductivity electrodes, system power. We explain the architecture of the system and required theory to implement the system. In order to investigate the clinical significance of the body composition data, compare to the data measured by the expert body composition analyzer which provide high reproduction and precision. Finally, experimental results which are the correlation between the measured data show the very high reproduction performance of the body composition analysis in the proposed system.