• Title/Summary/Keyword: Bd21-3

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Body Shape Classification of the Lower Body of Obese men in their 30's and 40's for Slacks Pattern Development (30~40대 비만 남성의 슬랙스 패턴개발을 위한 하반신 체형분류)

  • Sin, Sunmi;Do, Wolhee
    • Fashion & Textile Research Journal
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    • v.21 no.3
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    • pp.308-317
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    • 2019
  • This study provided data for classifying and characterizing the lower half of the body shape for obese adult men in their 30s and 40s. Data of 492 adult males who were obese with a WHO criteria of >25 BMI were used for analysis. The results of the study are as follows. Six factors extracted from the factorial analysis as independent factors for cluster analysis were classified into three types. Type 1 (65.4%) had the lowest height of the lower half of the body with short circumference and length. Type 2 (20.3%) had the lowest height of the lower half of the body with the largest thickness, width and circumference from the back to the hip, but short in length. Type 3 (14.2%) had the lowest height of the lower half of the body with medium height and waist-height; however, the curve from the waist to the hip was the largest with the largest waist circumference, hip circumference, and width and thickness of the lower half of the body. This study will help to design a slack pattern that utilizes body shape characteristics of men in their 30s and 40s. In a follow-up study, we analyze the slack pattern and educational pattern by the company and study the necessity for a slack pattern for obese males.

Gene Cloning, Purification, and Characterization of a Cold-Adapted Lipase Produced by Acinetobacter baumannii BD5

  • Park, In-Hye;Kim, Sun-Hee;Lee, Yong-Seok;Lee, Sang-Cheol;Zhou, Yi;Kim, Cheol-Min;Ahn, Soon-Cheol;Choi, Yong-Lark
    • Journal of Microbiology and Biotechnology
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    • v.19 no.2
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    • pp.128-135
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    • 2009
  • Acinetohacter baumannii BD5 was isolated from waters of Baek-du mountain, and the lipase gene was cloned using a PCR technique. The deduced amino acid sequence of the lipase and lipase chaperone were found to encode proteins of 325 aa and 344 aa with a molecular mass of 35 kDa and 37 kDa, respectively. The lipase gene was cloned and expressed in Escherichia coli BL21(trxB) as an inclusion body, which was subsequently solubilized by urea, and then purified using Ni-affinity chromatography. After being purified, the lipase was refolded by incubation at $4^{\circ}C$ in the presence of a 1:10 molar ratio of lipase:chaperone. The maximal activity of the refolded lipase was observed at a temperature of $35^{\circ}C$ and pH 8.3 when p-NP caprate(C10) was used as a substrate; however, 28% of the activity observed at $35^{\circ}C$ was still remaining at $0^{\circ}C$. The stability of the purified enzyme at low temperatures indicates that it is a cold-adapted enzyme. The refolded lipase was activated by $Ca^{2+},\;Mg^{2+},\;and\;Mn^{2+}$, whereas $Zn^{2+}\;and\;Cu^{2+}$ inhibited it. Additionally, 0.1% Tween 20 increased the lipase activity by 33%, but SDS and Triton X-100 inhibited the lipase activity by 40% and 70%, respectively.

Proteomic Profiles of Mouse Neuro N2a Cells Infected with Variant Virulence f Rabies Viruses

  • Wang, Xiaohu;Zhang, Shoufeng;Sun, Chenglong;Yuan, Zi-Guo;Wu, Xianfu;Wang, Dongxia;Ding, Zhuang;Hu, Rongliang
    • Journal of Microbiology and Biotechnology
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    • v.21 no.4
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    • pp.366-373
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    • 2011
  • We characterized the proteomes of murine N2a cells following infection with three rabies virus (RV) strains, characterized by distinct virulence phenotypes (i.e., virulent BD06, fixed CVS-11, and attenuated SRV9 strains), and identified 35 changes to protein expression using two-dimensional gel electrophoresis in whole-cell lysates. The annotated functions of these proteins are involved in various cytoskeletal, signal transduction, stress response, and metabolic processes. Specifically, a-enolase, prx-4, vimentin, cytokine-induced apoptosis inhibitor 1 (CIAPIN1) and prx-6 were significantly up-regulated, whereas Trx like-1 and galectin-1 were down-regulated following infection of N2a cells with all three rabies virus strains. However, comparing expressions of all 35 proteins affected between BD06-, CVS-11-, and SRV9-infected cells, specific changes in expression were also observed. The up-regulation of vimentin, CIAPIN1, prx-4, and 14-3-3 ${\theta}/{\delta}$, and down-regulation of NDPK-B and HSP-1 with CVS and SRV9 infection were ${\geq}2$ times greater than with BD06. Meanwhile, Zfp12 protein, splicing factor, and arginine/serine-rich 1 were unaltered in the cells infected with BD06 and CVS-11, but were up-regulated in the group infected with SRV9. The proteomic alterations described here may suggest that these changes to protein expression correlate with the rabies virus' adaptability and virulence in N2a cells, and hence provides new clues as to the response of N2a host cells to rabies virus infections, and may also aid in uncovering new pathways in these cells that are involved in rabies infections. Further characterization of the functions of the affected proteins may contribute to our understanding of the mechanisms of RV infection and pathogenesis.

A Study on the Foot Characteristics for the Development of the Last Footwear for Plus Size Women (플러스 사이즈 여성용 신발 라스트 개발을 위한 발 특성 연구)

  • Kim, Namsoon;Do, Wolhee
    • Fashion & Textile Research Journal
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    • v.22 no.3
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    • pp.378-385
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    • 2020
  • The purpose of this study was to present basic data for development of shoe last suitable for women of plus size (BMI 25 kg/㎡ or higher) by type and to analyze the characteristics of each type of foot type. The results of the study are as follows. A comparison of the feet of a plus-size woman and a normal-weight woman showed that the feet of a plussize woman were thicker and wider in the toes, cheeks and feet than those of a normal-weight woman, while the ankle area was thicker. As a result of the cluster analysis by type of foot type of plus-size woman, type 1 was classified into four types, type 1 was 49 (19.5%), type 2 was 43 (17.1%), type 3 was 53 (21.1%), and type 4 was distributed among 106 (42.2%). Type 1 appeared to be a long, low heel, thick foot and wide H-type, and Type 2 appeared to be a D-type with a low heel and a thinner and narrow foot compared to other types, but with wide outer width of the foot. Type 3 showed short feet, low heels, narrow feet, but slanted sides, and Type 4 showed type A with long feet, high heels, thick and wide feet or a combination of toes.

Analysis of Nucleotide Sequence Encoding VP2 Protein of Infectious Bursal Disease Virus Detected in Korea (국내 분리 닭 전염성 F낭병 바이러스의 VP2 단백질 생산 유전자의 염기서열 분석)

  • Kim, Toh-kyung;Yeo, Sang-geon
    • Korean Journal of Veterinary Research
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    • v.43 no.3
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    • pp.439-448
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    • 2003
  • The VP2 gene of infectious bursal disease virus (IBDV) Chinju which was previously detected in Chinju, Korea was cloned and sequenced to establish the information for the development of genetically engineered vaccines and diagnostic reagents against IBDV. The nucleotide sequence of the entire Chinju VP2 gene consisted of 1,356 bases long encoding 452 amino acids in a single open reading frame (ORF). It consisted of 368 adenine (27.1%), 363 cytosine (26.8%), 339 guanine (25.0%) and 286 thymine (21.1%) residues. The predicted $M_r$ of the Chinju VP2 protein was 48 kDa, and the protein contained 13 phosphorylation sites by protein kinase C, casein kinase II or tyrosine kinase, whereas 3 asparagine-linked glycosylation sites were recognized. The nucleotide sequence of Chinju VP2 ORF had a very close phylogenetic relationship with 98-99% homology to that of the very virulent IBDVs (vvIBDVs) HK46, OKYM, D6948, UK661, UPM97/61 and BD3/99. Also, the Chinju VP2 protein revealed a very close phylogenetic relationship with 99-100% homology to that of these vvIBDVs. The Chinju VP2 protein had 100% amino acid identity in the variable region of residues 206-360 with that of the D6948, HK46, OKYM and UK661, as well as 100% identity in two hypervariable regions of residues 212-224 and 314-324 with those of the D6948, HK46, OKYM, UK661, UPM97/61 and BD3/99. The amino acid sequence of the chinju VP2 protein contained a serine-rich heptapeptide of SWSASGS as in these vvIBDVs.

An HEVC intra encoder sharing DCT with RDO for a low complex hardware (하드웨어 복잡도를 줄이기 위한 RDO내 DCT 공유구조의 HEVC 화면내 예측부호화기)

  • Lee, Sukho;Jang, Juneyoung;Byun, Kyungjun;Eum, Nakwoong
    • Smart Media Journal
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    • v.3 no.4
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    • pp.16-21
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    • 2014
  • HEVC is the latest joint video coding standard with ITU-T SG16 WP and ISO/IEC JTC1/SC29/WG11. Its coding efficiency is about two times compared to H.264 high profile. Intra prediction has 35 directional modes including dc and planer. However an accurate mode decision on lots of modes with SSE is too costly to implement it with hardware. The key idea of this paper is a DCT shared architecture to reduce the complexity of HEVC intra encoder. It is to use same DCT block to quantize as well as to calculate SSE in RDO. The proposed intra encoder uses two step mode decision to lighten complexity with simplified RDO blocks and shares the transform resources. Its BD-rate increase is negligible at 20% on hardware aspect and the operating clock frequency is 300MHz@60fps on FHD ($1920{\times}1080$) image.

A Study on the Thermal Characteristics Comparison of the LED Floodlight Luminaire using Vapor Chamber Manufacturing Technology (베이퍼챔퍼 제조기술을 적용한 LED 투광등기구의 열 특성 비교에 관한 연구)

  • Seo, Jin-Kook;Yu, Young-Moon
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.29 no.1
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    • pp.15-21
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    • 2015
  • The purpose of this paper is to analyze thermal characteristics of the heat sinks to maximize the thermal diffusivity for LED floodlight. The 2 kind of samples were prepared by vapor chamber manufacturing technology using the heat pipe principle. It was analyzed the maximum temperature reduction effect and the thermal diffusion from the heat source depending on the types of chambers with 3 kind of working fluids. As a result, it was confirmed that thermal conductivity 23% increased, GVC-R type than IVC-R type.

Cloning and Expression of $\beta$-l,4-Glucosidase Gene from Pseudomonas sp. in Escherichia coli and Bacillus subtilis (Pseudomonas sp. $\beta$-1,4-Glucosidase 유전자의 Esherichia coli와 Bacillus subtilis에의 Cloning 및 발현)

  • 김양우;전성식;김석재;정영철;성낙계
    • Microbiology and Biotechnology Letters
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    • v.21 no.2
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    • pp.113-118
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    • 1993
  • Fro the purpose of producing glouse from cellobiose or oligo saccharide and obtaining genetic information of beta-1,4-glucosidase gene, alpha beta-1,4-glucosidase gene of Pseudomonas sp. LBC505, potent cellulase complex and xylanase producing strain, was cloned in Esherichia coli and Bacillus subtilis into pUC19 and pBD64, respectively. Recombinant plasmid pGL1 contained 1.2kb EcoRI fragment was isolated from transformants forming blue color around colony on LB agar plate containing 20 ng/ml of 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside(X-glu) and ampicillin.

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Effects of Substrate Size on the Growth of 4 Microphytobenthos Species (Achnanthes sp., Amphora sp., Navicula sp. and Nitzschia sp.) (저서미세조류 4종(Achnanthes sp., Amphora sp., Navicula sp. 그리고 Nitzschia sp.)의 성장에 영향을 미치는 부착기질 크기의 영향)

  • Kwon, Hyeong-Kyu;Yang, Han-Soeb;Yu, Yeoung-Moon;Oh, Seok-Jin
    • Journal of Environmental Science International
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    • v.21 no.1
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    • pp.105-111
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    • 2012
  • The effects of substrate size on the growth of microphytobenthos Achnanthes sp., Amphora sp., Navicula sp. and Nitzschia sp. were examined using glass beads in order for phytoremediation in the benthic layer of coastal waters. The glass beads used in this study were 0.09~0.15 mm (G.B 1), 0.25~0.50 mm (G.B 2), 0.75~1.00 mm (G.B 3) and 1.25~1.65 mm (G.B 4). No addition of glass bead used as control. The specific growth rate and maximum cell density of four microphytobenthos species were increasing with decreasing size of glass beads. Moreover, the control experiment without added attachment substrates showed the lowest specific growth rate and maximum cell density. Therefore, the suitable attachment substrates for mass culture of microphytobenthos seems to be important in order for phytoremediation using microphytobenthos.

A Perceptual Rate Control Algorithm with S-JND Model for HEVC Encoder (S-JND 모델을 사용한 주관적인 율 제어 알고리즘 기반의 HEVC 부호화 방법)

  • Kim, JaeRyun;Ahn, Yong-Jo;Lim, Woong;Sim, Donggyu
    • Journal of Broadcast Engineering
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    • v.21 no.6
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    • pp.929-943
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    • 2016
  • This paper proposes the rate control algorithm based on the S-JND (Saliency-Just Noticeable Difference) model for considering perceptual visual quality. The proposed rate control algorithm employs the S-JND model to simultaneously reflect human visual sensitivity and human visual attention for considering characteristics of human visual system. During allocating bits for CTU (Coding Tree Unit) level in a rate control, the bit allocation model calculates the S-JND threshold of each CTU in a picture. The threshold of each CTU is used for adaptively allocating a proper number of bits; thus, the proposed bit allocation model can improve perceptual visual quality. For performance evaluation of the proposed algorithm, the proposed algorithm was implemented on HM 16.9 and tested for sequences in Class B and Class C under the CTC (Common Test Condition) RA (Random Access), Low-delay B and Low-delay P case. Experimental results show that the proposed method reduces the bit-rate of 2.3%, and improves BD-PSNR of 0.07dB and bit-rate accuracy of 0.06% on average. We achieved MOS improvement of 0.03 with the proposed method, compared with the conventional method based on DSCQS (Double Stimulus Continuous Quality Scale).