• KSBB Journal
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• v.15 no.6
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• pp.573-577
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• 2000

The optimum fermentation conditions for the production of cellulose by a newly isolated Acetobacter sp. A9 were determined in static cultures. The strain was able to produce cellulose at $25-30^{\circ}C$ with a maximum at $30^{\circ}C$. Cellulose production occurred at pH 6.5-8.0 with a maximum at pH 6.5. The optimal culture medium was found to consists of 1.0% glucose, 1.0% yeast extract, 0.7% polypeptone, 0.15% acetic acid and 0.02% succinic acid. Cellulose production by Acetobacter sp. A9 followed the growth curve. Highest cellulose production, under optimum conditions, was $24.1m^2$, although this strain typically produced only $12.1 g/m^2$ in the basic medium. Cellulose production also depended on the depth and volume of the medium.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.3
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• pp.410-415
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• 2010

The purpose of this study was to investigate the anti-inflammatory effects of aqueous extract from Belamcanda chinensis (BC) on the RAW 264.7 cells. To evaluate the anti-inflammatory effects of BC, we examined the cytokine productions including nitric oxide (NO), interleukin (IL)-1b, IL-6 and tumor necrosis factor-a (TNF-a) in lipopolysaccharide (LPS)-induced RAW 264.7 cells and also inhibitory mechanisms such as mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-kB) using Western blot. BC inhibited LPS-induced production of NO, IL-6 and TNF-a but not of IL-1b in RAW 264.7 cells. BC respectively inhibited the activation of MAPKs such as c-Jun NH2-terminal kinase (JNK) and p38 but not of extracelluar signal-regulated kinase (ERK 1/2) and NF-kB in the LPS-stimulated RAW 264.7 cells. Taken together, Our results showed that BC down-regulated LPS-induced NO, IL-6 and TNF-a productions mainly through JNK and p38 MAPK pathway.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.9 no.1
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• pp.47-53
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• 1998

The purpose of this study was to investigate the possibility of providing familiar tasks as a treatment option to decrease echolalia. Two comparisons were made：One was to compare ‘conversation condition’ and ‘task performance condition.’ and the other was to compare ‘task performance alone condition’ and ‘task performance along with contingency of reinforcement condition.’ Two echolalic children aged 12 and 13 years participated in the experiment and A-B-A-B-BC-B-BC design was used, in which A was conversation only, B was task performance, and C was task performance along with contingency of reinforcement. In the A condition, the therapist asked easy and short questions to the child；in the B condition the child was given familiar tasks with short instruction, and in BC condition, each child was reinforced for his performance on given tasks, in which immediate echolalia was controlled through his hands being held down for 5 seconds. Delayed echolalia was recorded without any intervention being given. Each child was put into each of the 7 treatment conditions. With a 15 minutes session, each child went through 5 to 6 sessions per day for 2 weeks. The mean echolalia(immediate) rates across the 7 treatment conditions were：For child 1, A(99%)-B(65%)-A(95%)-B(10%)-BC(7%)-B(6%)- BC(7%) and for child 2, A(67%)-B(62%)-A(63%)-B(35%)-BC(8%)-B(4%)-BC(0%). As to the generalization of the treatment effect of immediate echolalia to the untreated delayed echolalia, there was shown a drastic reduction of delayed echolalia in child 2：A(35%)-B(57%)-A(56%)-B(40%)-BC(8%)-B(5%)-BC(9%). Child l’s delayed echolalia was negligible(mean=3%) pre-and post treatments. In conclusion, the results of this study clearly show that providing a task performance setting with familiar tasks can certainly be helpful for minimizing echolalic response, and along with the use of the contingency of reinforcement technique it can further not only correct echolalic behavior to a negligible degree but also help the echolalic child generalize its treatment effect to the child’ overall language improvement.

• Korean Journal of Agricultural Science
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• v.41 no.1
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• pp.1-7
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• 2014

Ratooning ability is one of the major different traits from perennial to cultivated rice and annual type. We developed a set of 126 introgression lines derived from a cross between Hwayeong and W1944 (O. rufipogon) to gain an insight into the genetic factors underlying differences between common wild rice and cultivated rice. One IL, CR6 among the 126 ILs of $BC_3F_4$ showed a significant difference in rationing ability compared with Hwayeong. To further characterize the rationing ability, CR6 was selected and crossed to Hwayeong to produce three secondary populations, $BC_4F_2$, $BC_4F_3$ and $BC_5F_2$. In the Hwayeong background, the W1944 allele was associated with an increase in rationing ability. QTL analysis showed that the qRAT5 for rationing ability was linked to RM194 ($R^2$=6.6%, 19.6%, and 44.5% in the $BC_4F_2$, $BC_5F_2$, and $BC_5F_3$, respectively). The putative qRAT5 was also tightly linked to QTLs for spikelets per panicle and grain weight indicating that this region harbors a QTL cluster related to domestication. To our knowledge, this is the first report to map the major QTL for ratooning ability in rice. The SSR markers linked to qRAT5 would be useful in marker-assisted selection for breeding lines with enhanced ratooning ability.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.131-135
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• 2002

Acetobacter xylinum KJ1 efficiently producing bacterial cellulose(BC) in shaking culture was isolated from a rotten grape. The strain was used to investigate optimum operating conditions for increasing BC production and factorial design model was employed for the optimization. The results of experiments were statistically analyzed by SAS program. Reciprocal effects of each factors(carbon source concentration, shaking speeds(rpm), oxygen pressure, and CSL concentration) and culture condition of BC production were examined by getting regression equation of the dependent variable. Comparisons between experimental results and predicted results about BC concentration were done in total 24 experiments by combination of each factors using SAS program, and the correlation coefficients of BC concentration and BC yield were 0.91 and 0.81, respectively. The agitated cultures were performed in various operation conditions of factors which affected considerably to BC production in jar fermentor. The results showed that BC concentration was 11.67g/ L in 80 hours cultivation under the condition of carbon source concentration shaking speeds(rpm) : oxygen pressure: CSL concentration = 4% : 460rpm : 0.28 : 6%. On the other hand BC yield was 0.42g/g in 80 hours cultivation under the condition of carbon source concentration shaking speeds(rpm) : oxygen pressure: CSL concentration = 4% : 564rpm : 0.21 : 2%. The BC production could be enhanced up to more than 65.3% by factorial design. The result of a verifying experiment under the optimal conditions determined by the factorial design to the BC production showed that the model was appropriate by obtaining BC concentration of 11.02g/L in the optimum condition

• The Korean Journal of Ecology
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• v.24 no.1
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• pp.51-59
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• 2001

Estimation of the annual net production, nitrogen (N) and base cation (BC＝K, Mg, Ca) uptake by stocked forests in South Korea has been done with national statistical data of forestry from 1995 to 1999. The annual net production of stems and branches was about 1.8 ton DM ha/sup -1/ yr/sup -1/. The net productivity of deciduous forests was higher than that of coniferous forests. Total net production of the stocked forests from the whole stocked area of South Korea of 6.246×10/sup 6/ ha, was about 1.13×10/sup 7 ton DM/yr, and the total harvested biomass obtained from timber production data in 1999, was estimated about 6.1×105 ton DM/yr that was equivalent to 98 kg DM ha/sup -1/ yr/sup -1/f. Net growth uptake of N and BC were 350 mol ha/sup -1/ yr/sup -1/ and 296 mol ha/sup -1/ yr/sup -1/ respectively, and the content of N and BC contained in the harvested biomass were 20 mol ha/sup -1/ yr/sup -1/ and 16 mol ha/sup -1/ yr/sup -1/ respectively. Net uptake of N was higher than that of BC. Total net uptake of N and BC from growth and harvest by stocked forests in South Korea were 2.309×10/sup 9/ mol/yr and 1.953×10/sup 9 mol/yr respectively.

• Korean Journal of Microbiology
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• v.46 no.2
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• pp.213-218
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• 2010

Lactate dehydrogenases (LDHs) have been highly focused for long time, due to their important roles in biochemical and metabolic pathways of cells. On the basis of genome-wide searching results, three putative LDH genes from Bacillus cereus ATCC 14579 genome have been PCR-amplified, cloned, and well-expressed in E. coli. All three BcLDH isozymes are supposed to share highly conserved catalytic amino acid residues in common $NAD^+$-dependent LDHs. Meanwhile, BcLDH1 consisting of 314 amino acids shares 86 and 49% of identities with BcLDH2 and 3, respectively. Interestingly, only BcLDH1 showed the converting activities between L-lactate and pyruvate in the presence of $NAD^+$ coenzyme, while the other isozymes are likely to have almost no activity. As a result, it was revealed that BcLDH1 can be a typical $NAD^+$-dependent L-lactate-specific dehydrogenase.

• Journal of Ginseng Research
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• v.47 no.6
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• pp.773-783
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• 2023

Background: Gray mold, caused by Botrytis cinerea, is one of the major fungal diseases in agriculture. Biological methods are preferred over chemical fungicides to control gray mold since they are less toxic to the environment and could induce the resistance to pathogens in plants. In this work, we try to understand if ginseng defense to B. cinerea could be induced by fungal hypovirulent strain △BcSpd1. BcSpd1 encodes Zn(II)₂Cys₆ transcription factor which regulates fungal pathogenicity and we recently reported △BcSpd1 mutants reduced fungal virulence. Methods: We performed transcriptomic analysis of the host to investigate the induced defense response of ginseng treated by B. cinerea △BcSpd1. The metabolites in ginseng flavonoids pathway were determined by UPLC-ESI-MS/MS and the antifungal activates were then performed. Results: We found that △BcSpd1 enhanced the ginseng defense response when applied to healthy ginseng leaves and further changed the metabolism of flavonoids. Compared with untreated plants, the application of △BcSpd1 on ginseng leaves significantly increased the accumulation of p-coumaric acid and myricetin, which could inhibit the fungal growth. Conclusion: B. cinerea △BcSpd1 could effectively induce the medicinal plant defense and is referred to as the biological control agent in ginseng disease management.

• Horticultural Science & Technology
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• v.18 no.3
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• pp.342-347
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• 2000

The objectives of this study were to investigate the genetic and phenotypic features of male sterile transformants by pollen-specific expression of diphtheria toxin gene and to find out inheritance patterns of transgene to the next generation. When backcrossed (BC) progenies were tested for expression of kanamycin resistance ($Km^R$), 9 lines out of 13 lines, except 4 lines ($BC_{1}5-13,\;BC_{1}5-23,\;BC_{1}5-28,\;BC_{1}5-32$), showed the ratio of $Km^R$ to kanamycin sensitive ($Km^S$), from 1:30 to all $Km^S$. As a result, they were much lower than Mendelian segregation of a dominant gene. To determine whether male sterility is a heritable and stable trait, 5 male sterile plants ($BC_{1}5-13,\;BC_{1}5-14,\;BC_{1}5-23,\;BC_{1}5-32,\;BC_{1}5-33$ lines) which had different transgene copy numbers were backcrossed as female parents with pollens from wild type. To confirm the existence of the DTx-A gene in the genome of the progenies, PCR was conducted using specific primers of the DTx-A coding region. A PCR band of 428 bp was obtained from each generation, which is the predicted size of the DTx-A gene fragment. Trangenes were inherited to the next $BC_4T_0$ progenies and showed male sterility, however, based on the copy numbers of DTx-A gene male sterile plants did not show predicted ratio. When male sterile plants were backcrossed with fertile plants, fruit capsule sizes and seed settings were relatively reduced from those of selfing wild type plants. The fruit sizes and seed settings were reduced in proportion to the increase in the copy number of DTx-A gene.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.5A
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• pp.490-496
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• 2007

This paper describes the design and implementation of an integrated LTCC front-end module for the IEEE802.11a WLAN applications by performing the behavioral-level simulation using measurement-based behavioral model. To meet the IEEE802.11a WLAN standard, a system transmitting 1024 symbols through 64-QAM process at the rate of 54Mbps should be implemented and nonlinear properties are confirmed by simulations of ACPR and EVM in this circumstance. The right offsets of ACPR which are 30MHz, 20MHz, and 11MHz distant from the center frequency of 5.8GHz are 49.36dBc, 36.90dBc, and 24.58dBc, respectively. The left offsets are 50.14dBc, 30.04dBc, and 28.85dBc, respectively and EVM is 2.94%. The size of the module implemented with LTCC five-layer substrates is $13.4mm{\times}14.2mm$. The measured characteristics of the transmitter show P1dB of 16.2dBm and power gain of 16.73dB. Those of the receiver exhibit the small signal gain of 16.24dB and noise figure of 7.83dB.