• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.208-216
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• 2010

Fusarium verticillioides is an important pathogen of maize, being responsible for ear rots, stalk rots, and seedling blight worldwide. During the past decade, F. verticillioides has caused several severe epidemics of maize seedling blight in many areas of China, which lead to significant losses. In order to understand the molecular mechanisms regulating fungal development and pathogenicity in this pathogen, we isolated and characterized the gene fpk1 (GenBank Accession No. EF405959) encoding a homolog of the cAMP-dependent protein kinase catalytic subunit, which included a 1,854-bp DNA sequence from ATG to TAA, with a 1,680-bp coding region, and three introns (lengths: 66 bp, 54 bp, and 54 bp), and the predicated protein precursor had 559 aa. The mutant ${\Delta}fpk1$, which was disrupted of the fpkl gene, showed reduced vegetative growth, fewer and shorter aerial mycelia, strongly impaired conidiation, and reduced spore germination rate. After germinating, the fresh hypha was stubby and lacking of branch. When inoculated in susceptible maize varieties, the infection of the mutant ${\Delta}fpk1$ was delayed and the infection efficiency was reduced compared with that of the wild-type strain. AU this indicated that gene fpk1 participated in hyphal growth, conidiophore production, spore germination, and virulence in F. verticillioides.

• Research in Plant Disease
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• v.17 no.1
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• pp.82-85
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• 2011

Bakanae disease symptom were observed in barnyard grass in paddy field in Heanam, Jeonnam. The infected plants were blighted and white mass of spore were formed on the stem. Fusarium species were isolated from infected stem and the isolates were identified as Fusarium fujikuroi based on their morphological and molecular characteristics. The isolates of F. fujikuroi were assigned to reference of F. fujikuroi among related Fusarium species based on the translation elongation factor 1-alpha gene sequence. Pathogenicity of the fungal isolates was confirmed on seedlings of rice and barnyard grass by artificial inoculation. The results indicated that barnyard grass can be inoculum source of Bakanae disease on rice. Thus, effective weed management is necessary to Bakanae disease control and healthy seed production.

• Proceedings of the Korea Water Resources Association Conference
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• 2006.05a
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• pp.462-466
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• 2006

Close-to-nature stream evaluation is one of the processing to make the streams over in order to keep them natural. It is integral to evaluate and make an accurate analysis of them on the purpose of maintaining streams healthy. For many instances, there are, stream organization evaluation for restoration by German government, evaluation for ecosystem protection in natural preserves by New Zealand government, and stream-view evaluation for restoration by Britain government so on. In case of the country there are analysis and evaluation of stream physical organization by Cho, Yong-hyun, Close-to-nature stream evaluation for restoration by Kim, Dong-chan, evaluation of stream properties in korea by Park, Bong-jin. Close-to-nature evaluation by Lim, Chan-uk, that is advanced version of Park, Bong-jin's, shows form of stream including waterway curve, sand bar, diversity of flow, river bed material, diversity of minor bed, minor bed bank protection works, bank protection material. It also does environment of stream including side of minor bed vegetation, width of surface of the water/width of the river etc.. By the way, this evaluation does not have free access to apply those details above in the field, it often happens that you get various outcome from the one spot. so you must need more realistic testing method to obtain more accurate data. Remote sensing method is highly recommended because this is very useful for collecting realistic data of vegetation index. what is more, it can not only scan even the minimum area within its resolving power but also do obtain data anytime. Vegetation index indicates Ratio vegetation index, Normalized difference vegetation index, Soil adjusted vegetation index, Atmospherically resistant vegetation index etc.. The research is focusing on Cheokgwa stream which is the branch of Taehwa river and shows 19 sectioned Close-to-nature stream performed according to the method by Lim, chan-uk. Besides let you know vegetation index came from image data of satellite landsat 7 with the variation of buffering area, of the day 9. may. 2003. Of all, the outcome 0.758 at 200m buffer-zone of NDVI was the best we have got so far.

• The Korean Journal of Mycology
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• v.39 no.3
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• pp.217-222
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• 2011

Job's tears (Coix lacryma-jobi) is native to East Asia, and grains of the plant are used as health food and medicinal material. Head blight symptoms of the plant were frequently observed during disease surveys in Korea from 2006 to 2008. The symptoms were characterized as discoloration of husks, and subsequently inside of mature grains were shriveled or emptied. One hundred fifty nine isolates of Fusarium species were obtained from the disease symptoms of the plant collected from several locations in the country. Out of the isolates, the most frequently isolated Fusarium species were F. graminearum (34%), F. proliferatum (14.5%), F. verticillioides (10.1%), F. equiseti (6.9%), and F. fujikuroi (6.3%). Other Fusarium species isolated were F. subglutinans, F. semitectum, F. poae, and F. sporodochioides. Elongation factor 1 alpha gene sequences of the isolates were used for phylogenetic analysis. Analyses of the sequences revealed that the isolates were confirmed to be identical with each reference species of NCBI GenBank. Pathogenicity tests showed that F. graminearum, F. proliferatum and F. verticillioides were strongly virulent to grains of Job's tears. The present study is the first report of head blight of Job's tears caused by Fusarium species in Korea.

• The Korean Journal of Mycology
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• v.46 no.1
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• pp.58-68
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• 2018

Sunn hemp (Crotalaria juncea) is used as a nitrogen-fixing green manure in Korea to improve soil quality, reduce soil erosion, and suppress weeds and nematodes. In 2014, wilting sunn hemp plants were observed in green manure-cultivated fields in Wanju, Korea. Leaves of the infected plants began yellowing, starting with the lower leaves, eventually leading to their death. Moreover, a number of dark perithecia were observed on the wilting stems. Six isolates were obtained from these perithecia by single spore isolation. Based on their morphological characteristics, the isolates were identified as Fusarium udum (teleomorph: Gibberella indica). Macroconidia were slightly curved with almost hooked apical cell, and microconidia were formed on false heads by monophialides. Chlamydospores were produced abundantly in the hyphae, either singly or in clusters. To confirm the identification, multilocus sequence analysis was conducted using translation elongation factor 1 alpha (TEF), calmodulin (CAL), and histone 3 (HIS3). The sequences of TEF, CAL, and HIS3 showed 94.4~96.2%, 99.7%, and 99.6~99.8% similarity to the reference sequences of F. udum in NCBI GenBank, respectively. Pathogenicity was tested on sunn hemp and two soybean cultivars using the inoculation method of soil drenching with spore suspension. The wilting symptoms were observed only in sunn hemp and one cultivar of soybean (cv. Teagwang) after 14~21 days of inoculation. This is the first report of wilt disease in sunn hemp caused by Fusarium udum in Korea.

• Journal of Korean Society of Disaster and Security
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• v.12 no.1
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• pp.23-34
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• 2019

It is an urgent issue to manage and reduce non-point pollution sources for improving the water quality of stream and lakes in rural areas. In this study, in order to reduce non-point pollution sources in rural area, Gabion mattresses was proposed to provide protection of riverbanks with anaerobic and aerobic area. The utilization of this was assessed by lab scale model test and pilot plant test. After filling the inside of the gabion mattresses with aggregate, the filtration zone under anaerobic and aerobic conditions was formed to treat the contaminants. In addition, vegetation was deposited on the surfae of the gabion to prevent the inflow of soil and to promote purification by the plant. COD and nitrogen content (T-N, $NH_4{^+}$, -N, $NO_3{^-}N$) were monitored in model and field tests. The lab scale model test showed removal efficiency of 17% of TCOD, 35% of SCOD, 14% of TN, 62% of $NH_4{^+}$, -N, and 33% of $NO_3{^-}$ N. Also, pilot plant test showed removal efficiency of 24% of TCOD, 29% of SCOD, 47% of TN, 50% of $NH_4{^+}-N$, 33% of $NO_3{^-}$, N and 29% of TP.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.5
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• pp.92-100
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• 2009

This study was surveyed from May to October, 2008 in order to classify type distribution and evaluate the function of inland wetland as a ecological axis in Chungnam province. Assessment was done by modified-RAM (Rapid Assessment Method). RAM is consisted of total 8 functions and divided into high, moderate, low. The conservation grade of RAM is divided into 4 grades; absolute conservation, conservation, improvement and restoration. Throughout survey on total 13 wetlands of Lacustrine, Palustrine wetland which are distributed in Chungnam province, their function was assessed. As result, the 2 wetlands were judged as absolute conservation grade by assessment of 8 functional contents, and 7 sites were improvement wetlands and 4 sites were conservation wetlands. The function of wetlands assessed as conservation grade showed high in water quality protection and improvement. Also, showed high in vegetation diversity, wildlife habitat and aesthetic recreation. Meanwhile, showed low in Water quality purification, Shoreline/Stream Bank Protection. Of wetlands evaluated as conservation grade, Jeong-juk Ji and Dun-ri reservoir were assessed as absolute conservative area. These wetlands are essential to be managed continuously as a area having high ecological value. Farther, these wetlands will be done as a axis of ecological network related to 'Kumbuk jeongmaek' ecosystem.

• International Commerce and Information Review
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• v.15 no.3
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• pp.245-262
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• 2013

The transaction size between South Korea and the European Union (EU) had increased by more than two times among 2003 to 2008. With rapid growth of transaction, the EU was becoming important transaction object of South Korea gradually. EU has used the Antidumping policy as a trade protection tool against Korean products due to reduce the deficit of trade balance of payment, boost the economic growth and protect its weak industries. This paper investigates whether there is a connection between the EU's macroeconomic activity and pressures for protection to Korean products under antidumping measures with using the current data that come from the WTO, World Bank for 2004 to 2012. The result suggests that pressures for protection under Antidumping measure against exporting of Korean products to EU have increased during periods of macroeconomic weakness of low GDP growth, larger deficit of trade balance, however, has not significantly affected during periods of high unemployment.

• International Commerce and Information Review
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• v.14 no.1
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• pp.3-22
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• 2012

China has used the Antidumping policy as a trade protection tool against Japanese products due to reduce the deficit of trade balance of payment, boost the economic growth and protect its weak industries. This paper investigates whether there is a connection between the China's macroeconomic activity and pressures for protection to Japanese products under antidumping measures with using the current data that come from the WTO, World Bank for 1997 to 2010. The result suggests that pressures for protection under Antidumping measure against exporting of Japanese products to China have increased during periods of macroeconomic weakness like high unemployment, larger deficit of trade balance and decreased during periods of macroeconomic strength.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.6
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• pp.70-80
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• 2007

This study was surveyed from January to september, 2007 in order to evaluate the function of coastal wetland as a ecological axis in korea peninsula. Assessment was done by RAM (Rapid Assessment Method). RAM is consisted of total 8 contents and divided into high, moderate, low. The preservation grade of RAM is divided into 4 grades; absolute preservation, preservation, improvement and improvement or restoration. Throughout survey on total 14 wetlands of marine, estuary wetland and back marsh which are distributed in west coast in chung-nam province, their function was assessed. As result, total all the 14 wetlands were judged as preservation grade by assessment of 8 functional contents. The function of wetlands assessed as preservation grade showed high in water quality protection and improvement. Also, showed high in vegetation diversity, wildlife habitat and aesthetic recreation. Meanwhile, showed low in ground water recharge, Shoreline/Stream Bank Protection, Flood/Stormwater storage and Flood flow alteration. Of wetlands evaluated as preservation grade, Dae-ho, Sinduri, Bu-Nam lake, Sowhang dune and keum river estuary were assessed as absolute preservative area owing to habitation of international protection species and endangered species. These wetlands are essential to be managed continuously as a area having high ecological value. Farther, this wetlands will be done as a axis of ecological network related to land ecosystem.