• 제목/요약/키워드: Baiting

검색결과 23건 처리시간 0.026초

맥주와 담배 혼합액을 이용한 민달팽이 방제 (Control of Slug by Using Beer and Cigarette Mixture)

  • 윤종철;박종호;심창기;류경열;지형진
    • 한국응용곤충학회지
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    • 제46권2호
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    • pp.325-330
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    • 2007
  • 다양한 엽채류에 발생하며 특히 상추에 피해가 큰 민달팽이는 여러 가지 식품자재 중 맥주와 오이즙액에 가장 효과적으로 유인되었다. 하지만, 이들 재료는 민달팽이를 유인할 뿐 살충효과는 전혀 없었다. 민달팽이 유인재료에 살충효과를 나타내는 물질들을 첨가하였을 때 맥주와 담배가루 혼합액의 민달팽이 유인 포살효과가 가장 높았다. 맥주 50ml에 담배 1개비 가루를 혼합하여 작은 플라스틱 용기에 담고 지표면에 매몰한 결과 하룻밤 사이에 평균 25 마리의 민달팽이가 유인 포살되는 것으로 나타났다. 하지만, 민달팽이 유인효과가 높았던 오이즙액에 담배가루를 혼합한 처리구에서는 평균 4.3마리만 유살되었다. 맥주와 담배 혼합액을 민달팽이가 심하게 발생된 상추 재배하우스에 3일간 연속으로 처리한 결과 68.4%의 방제효과를 나타내었으며, 상추 육묘상에서는 58.3%의 방제효과를 보였다. 본 연구에서 개발된 트랩은 매일 작업을 해야 하는 번거로움이 있으나 민달팽이의 피해가 큰 친환경 유기농 채소 생산농가의 육묘장이나 본포장 등 제한된 면적에서는 활용가치가 높을 것으로 기대된다.

목조건축물의 흰개미 방제에 대한 국외 현황조사(I) - 미국의 사례를 중심으로 - (Study of the present situation on the termite control of wooden structures(I) - Focused on the case of US)

  • 정소영
    • 보존과학연구
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    • 통권32호
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    • pp.123-136
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    • 2011
  • 우리나라에는 지중흰개미의 일종인 큐슈흰개미 1종이 분포하고 있으며, 흰개미 피해가 발견되었을 경우 훈증처리, 토양처리, 방충방부처리, 흰개미 군체제거시스템 등 4가지 방법을 적용하여 방제하고 있다. 그러나 점차적으로 흰개미에 의한 피해가 증가하고 있으므로 흰개미 피해가 심각한 미국, 일본 등 다른 나라의 피해 현황 및 방제 방법에 대해서도 조사할 필요가 있다. 본 고에서는 1950년경부터 흰개미 피해가 발생한 미국을 중심으로 흰개미 분포 현황 및 방제 체계에 대해 살펴보고자 한다. 미국에는 지중흰개미, 건재흰개미, 습재흰개미 등 다양한 종류의 흰개미가 분포하고 있어, 우리나라에 비해 흰개미에 의한 피해가 더 심하게 발생한다. 따라서 흰개미 피해를 조사하고 적절한 방제방법을 선택하는 것이 중요한데, 그 과정은 흰개미 피해 조사, 화학적 방제처리(토양 약제 처리, 흰개미 군체제거시스템), 건축물 유지보수, 주기적인 모니터링의 순서로 이루어진다. 그리고 분포하는 흰개미의 종류가 다양하기 때문에 흰개미 피해로부터 방제작업을 진행할 때도 흰개미의 특성을 고려하여 각각 다르게 적용된다. 지중흰개미에 의한 피해가 발견되었을 경우에는 건축물에 대한 기피성 또는 비기피성 살충제를 이용하여 처리하거나 흰개미 군체제거시스템을 이용하여 처리한다. 반면, 건재흰개미에 의한 피해가 발생한 경우에는 훈증처리,열처리 등을 통해 방제하고 있다.

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An Oligonucleotide Microarray Bait for Isolation of Target Gene Fragments

  • Shi, Rong;Ma, Wen-li;Liu, Cui-Hua;Song, Yan-Bin;Mao, Xiang-Ming;Zheng, Wen-Ling
    • BMB Reports
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    • 제37권2호
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    • pp.148-152
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    • 2004
  • A new molecular-baiting method was studied by retrieving targeted gene fragments from an oligonucleotide microarray bait after hybridization. To make the microarray bait, 70-mer oligonucleotides that were designed to specifically represent the SSA1 gene of Saccharomyces cerevisiae were printed on the slide. Samples of the Saccharomyces cerevisiae mRNA were extracted and labeled by the RD-PCR (Restriction Display PCR) method using the Cy5-labelled universal primer, then applied for hybridization. The sample fragments that hybridized to the microarray were stripped, and the eluted cDNAs were retrieved and cloned into the pMD 18-T vector for transformation, plasmid preparation, and sequencing. BLAST searching of the GenBank database identified the retrieved fragments as being identical to the SSA1 gene (from 2057-2541bp). A new method is being established that can retrieve the sample fragments using an oligo-microarray-bait.

Ingestion Toxicity of Fipronil on Reticulitermes speratus kyushuensis (Isoptera: Rhinotermitidae) and Its Applicability as A Termite Bait

  • Kim, Si Hyun;Chung, Yong Jae
    • Journal of the Korean Wood Science and Technology
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    • 제45권2호
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    • pp.159-167
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    • 2017
  • Historical wooden buildings in Korea are being damaged by Reticulitermes speratus kyushuensis Morimoto, a type of subterranean termite, and the scale of this damage is increasing gradually because of global warming. This study evaluated the ingestion toxicity of the phenylpyrazole insecticide fipronil to R. s. kyushuensis and its applicability as termite bait with the aim of controlling termite colonies more efficiently. An ingestion toxicity assessment was conducted and the $LT_{50}$ was determined to be 4.43 day at concentrations of 10 ppm, indicating a slow-acting effect; therefore, 10 ppm was selected as an appropriate dose. A field applicability assessment was conducted in which the number of foraging workers and the rate at which the termites fed decreased 2 weeks after baiting, and termite colony activity was no longer apparent after 4 weeks demonstrating the efficacy of fipronil at eliminating colonies. Taken together, these results, indicate that low-dose fipronil eliminated R. s. kyushuensis colonies faster than insect growth regulators; therefore, it is expected to be useful when trying to conserve historical wooden buildings.

Malaria transmission potential by Anopheles sinensis in the Republic of Korea

  • Lee, Hee-Il;Lee, Jong-Soo;Shin, E-Hyun;Lee, Won-Ja;Kim, Yoon-Young;Lee, Kyung-Ro
    • Parasites, Hosts and Diseases
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    • 제39권2호
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    • pp.185-192
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    • 2001
  • To evaluate the factors that determine the transmission level of vivax malaria using vectorial capacity, entomological surveys were conducted from .lune to August, 2000. From 6 nights of human-bait collection in Paju, the human biting rate (ma) was counted as 87.5 bites/man/night. The parity of Anopheles sinensis from human baiting collections fluctuated from 41% to 71% (average 48.8%) of which the rate gradually increased as time passed on: 35.2% in Jun. ; 55.0% in July; 66.2% in Aug. From this proportion of parous, we could estimate the probability of daily survival rate of An. sinensis to be 0.79 assumed with 3 days gonotrophic cycle and the expectancy of infective life through 11 days could be defined as 0.073. Blood meal analysis was performed using ELISA to determine the blood meal source. Only 0.8% of blood meals were from human hosts. We could conclude that An. sinensis is highly zoophilic (cow 61.8%) Malaria is highly unstable (stability index < 0.5) in this area. From these data, vectorial capacity VC) was determined to be 0.081. In spite of a high human biting rate (ma), malaria transmission potential is very low due to a low human blood index. Therefore, we could conclude that malaria transmission by An. sinensis is resulted by high population density, not by high transmission potential. For this reason, we need more effort to decrease vector population and vector-human contact to eradicate malaria in Korea.

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전주근교의 토양에서 분리된 호각화질성 진균의 오염에 관한 조사 (The Isolation of Keratinophilic Fungi from Soils in Chon-ju Area)

  • 허인;이주묵;윤창모
    • 한국임상수의학회지
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    • 제7권2호
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    • pp.489-495
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    • 1990
  • The purpose of this study was to investigate the contamination rates of Keratinophilic fungi in soils In Chonju. From september to November 1986, 98 soil samples were collected at the school and children's play ground, dog barn, pig barn, cow barn, horse barn and fowl barn( total 33 sites ). The samples were collected at the different depths(0~2cm, 30cm, 50cm ) in each of the sites, respectively. Each sample was cultured at 25$^{\circ}C$ according to Modified hair baiting method by using horse mane hair as a bait. The results obtained were summerized as follows ; 1. Eighty one of the 98 soil samples were found to be positive for Keratinophilic fungi. 2. In the examination of keratinophilic fungi in 98 soil samples, the organisms isolated weie as follows; Microsporum gypseum 20, Trichophyton ajelloi 15, Chrysosporium tropicum 40, C. Keratinophilum 24, C. tuberculatum 6 and Chrysosporium spp. like organism 4 strains. 3. The positive rates of keratinophilic fungi in each of the depths were 87.9%(29/33) in surface layer, 90.9%(30/33) in middle layer and 68.8%(22/32) in deep layer. 4. The positive rates of Keratinophilic fungi in each of the sites were 100%(3/3) in horse barn 91.7% (l1/12) in dog barn, 88.9%(16/18) in pig barn, 86.7%(13/15) in cow barn, 76.2%(16/21) in fowl barn and 75.9%(22/29) in school and children's play ground. 5. The isolation rates of M. gypseum, Pathogenic fungi for human and animals, were as follows ; 58.3% in dog barn(surface layers 100%, middle layers 50%, deep layers 25%) and 16.7% in pig barn(surface 33.3%, middle 16.7%, deep 0%). In the cow barn, the isolation rates were 13.3%(surface 40.0%, middle 0%, deep 0%) and 10% in horse barn(surface l00%, middle 100%, depp 100%). In the fowl barn, the isolation rates was 19.1%(surface 28.8%, middle 14.3%, deep 14.3%) and 3.5% in school and children's play ground(surface 0%, middle 0%, deep 11.1%).

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솔잎 혹파리의 병원미생물에 관한 조사연구 I. 특히 가잠경화병과 관련하여 (Studies on the Investigation of Microbial Pathogens of Pine Needle Gall Midge, Thecodiplosis japonensis Uchida et Inoue I. Pathogenecity of Silkworm Muscardine to Pine Needle Gall Midge)

  • 강석권;조용섭;박호용;고성철
    • 한국잠사곤충학회지
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    • 제24권1호
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    • pp.32-38
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    • 1982
  • 본시험은 솔잎혹파리의 미생물학적 방제대책의 일관으로서 미생물의 분리동정과 병원성을 연구 조사하고자 행하여졌다. 1. 솔잎혹파리의 유충에서 분리동정 된 균은 Verticillium psalliotae, Aureobdsidium pullulansa, Streptomyces sp., Aspergillus sp.(2종), Ascochyt, sp.의 6종이었으며 6종 전부가 병원성이 있었다. 2. 추기 양잠농가로부터 Spicaria pracina, Isaria farinosa, Oospora destructor, Aspergillus oryzae, A. flavus의 5종의 누에 경화병균을 분리 동정하여, 솔잎혹파리유충에 대한 병원성을 검정한 결과, 5종 전부가 강력한 병원성을 나타내었다. 3. 솔잎혹파리 유충에서는 29종의 colony가 나타났으며 그 중에서 Bacillus 계통이 11, coccus 계통이 9개로 구분되었으며, 현재 병원성검정은 진행 중에 있으나 No.4와 No.9-1균주는 병원성이 인정되었다.

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Detection of vivax malaria sporozoites naturally infected in Anopheline mosquitoes from endemic areas of northern parts of Gyeonggi-do (Province) in Korea

  • Lee, Hyeong-Woo;Shin, E-Hyun;Cho, Shin-Hyeong;Lee, Hee-Il;Kim, Chung-Lim;Lee, Wook-Gyo;Moon, Sung-Ung;Lee, Jong-Soo;Lee, Won-Ja;Kim, Tong-Soo
    • Parasites, Hosts and Diseases
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    • 제40권2호
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    • pp.75-81
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    • 2002
  • We investigated population densities of mosquitoes infected with sporozoites in three highly epidemic areas of Josan-ri and Jangpa-ri (Paju City) and Dongjung-ri (Yeoncheon County) in Korea. Anopheline mosquitoes were collected front both indoors and outdoors by human baiting collection method during the period of the first week of June to the second week of September 1999. Total 13,296 female mosquitoes were collected and 8,650 (65.1%) were Anophelines. Thirty seven percent (3,199) of the Anopheline mosquitoes were captured outdoors and 63.9% (5,531) indoors. Employing a sandwich enzyme-linked immunosorbent assay (ELISA), we analyzed a total of 7,820 Anopheline mosquitoes and found that 7 Anopheline mosquitoes were infected with sporozoites. The positive rate in Josan-ri was 0.14% (5/3,500) and 0.15% (2/1,370) in Jangpa-ri. The total positive rate in all three surveyed areas was 0.09% (7/7,820). The mosquitoes infected with the sporozoites were detected on June $28^{th}$ (n=2), July $5^{th}$ (n=1), July $19^{th}$ (n=1), August $9^{th}$ (n=1), September $6^{th}$ (n=1), and the last one on September $13^{th}$ (n=1). They were all classified as Anopheles sinensis, which showed positive reaction in ELISA test. Therefore it might be concluded that Anopheles sinensis plays an important role in re-emerging malaria transmission in Korea.

Isolation and Identification of Burkholderia pyrrocinia CH-67 to Control Tomato Leaf Mold and Damping-off on Crisphead Lettuce and Tomato

  • Lee, Kwang-Youll;Kong, Hyun-Gi;Choi, Ki-Hyuck;Lee, Seon-Woo;Moon, Byung-Ju
    • The Plant Pathology Journal
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    • 제27권1호
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    • pp.59-67
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    • 2011
  • A bacterial strain CH-67 which exhibits antagonism towards several plant pathogenic fungi such as Botrytis cinerea, Fulvia fulva, Rhizoctonia solani, Sclerotinia sclerotiorum, Colletotrichum sp. and Phytophthora sp. was isolated from forest soil by a chitin-baiting method. This strain was identified as Burkholderia cepacia complex (Bcc) and belonging to genomovar IX (Burkholderia pyrrocinia) by colony morphology, biochemical traits and molecular method like 16S rRNA and recA gene analysis. This strain was used to develop a bio-fungicide for the control of tomato leaf mold caused by Fulvia fulva. Various formulations of B. pyrrocinia CH-67 were prepared using fermentation cultures of the bacterium in rice oil medium. The result of pot experiments led to selection of the wettable powder formulation CH67-C containing modified starch as the best formulation for the control of tomato leaf mold. CH67-C, at 100-fold dilution, showed a control value of 85% against tomato leaf mold. Its disease control efficacy was not significantly different from that of the chemical fungicide triflumidazole. B. pyrrocinia CH-67 was also effective in controlling damping-off caused by Rhizoctonia solani PY-1 in crisphead lettuce and tomato plants. CH67-C formulation was recognized as a cell-free formulation since B. pyrrocinia CH-67 was all lethal during formulation process. This study provides an effective biocontrol formulation of biofungicide using B. pyrrocinia CH-67 to control tomato leaf mold and damping-off crisphead lettuce and tomato.

풀무치에 대하여 살충활성을 보유한 곤충병원성 진균의 생리활성 평가 (Assessment of Physiological Activity of Entomopathogenic Fungi with Insecticidal Activity Against Locusts)

  • 이미롱;김종철;이세진;김시현;이석주;박소은;이왕휴;김재수
    • 한국응용곤충학회지
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    • 제56권3호
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    • pp.301-308
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    • 2017
  • 풀무치 (Locusta migratoria) (Orthoptrea: Acridiade)는 전 세계적으로 작물 생산에 심각한 문제를 야기하는 돌발 해충이다. 그러나 우리나라의 경우 풀무치를 방제하기 위한 방제제 및 적용에 대한 연구는 미흡한 실정이다. 본 연구에서는 풀무치에 병원성을 갖는 풀무치병원성 진균 라이브러리를 구축하였으며, 풀무치 방제에 이용 가능한 생물학적 방제제로서의 가능성을 평가 하였다. 먼저 갈색거저리 유충-baiting 시스템을 이용하여 다양한 지역에서 채집된 토양에서 곤충병원성 진균을 발굴 하였다. 풀무치 병원성 검정을 진행하기 위하여 국립 농업 과학원에서 풀무치를 분양 받았으며, 고체 배양된 곤충병원성 진균을 곤충 사육 상자에 처리하여 (2 g/box), 풀무치 약충 (3-4령충)에 대한 곤충병원성 진균의 병원성을 평가 하였다. 그 결과 곤충병원성 진균 처리 3-7일차에 풀무치의 머리, 복부, 다리 표면에서 진균이 증식하는 mycosis를 확인 할 수 있었다. 특히, Metarhizium anisopliae, M. lepidiotae, Clonostachys rogersoniana에서 높은 병원성이 나타나는 것이 확인 되었다. 확보된 34개의 풀무치병원성균주의 특성을 파악하기 위하여 열안정성 및 포자생산성을 확인 한 결과, Paecilomyces, Purpureocillium 균주가 다른 균주에 비해 열에 대한 높은 안정성안 나타나는 것을 확인 하였으며, 대부분의 균주에서 $1{\times}10^8conidia/gram$ 이상의 포자수를 생산 하는 것을 확인 하였다. 또한 온실 조건에서 비교적 병원성이 높았던 M. anisopliae 고체 배양된 균주를 토양에 처리하여 병원성을 확인한 결과, 85.7%의 높은 방제효과를 확인 할 수 있었다. 본 실험을 통하여 풀무치가 이동하면서 토양에 정착된 곤충병원성 진균에 접촉되어 치사 될 수 있을 것으로 판단되며, 효과적인 풀무치 방제가 가능 할 것이라고 판단된다.