Studies on the Investigation of Microbial Pathogens of Pine Needle Gall Midge, Thecodiplosis japonensis Uchida et Inoue I. Pathogenecity of Silkworm Muscardine to Pine Needle Gall Midge

솔잎 혹파리의 병원미생물에 관한 조사연구 I. 특히 가잠경화병과 관련하여

The study has been carried out to investigate a possibility to control the pine needle gall midge, Thecodiplosis japonensis Uchida et Inoue, by microbial pathogens as one of the microbial control measures. The samples were collected at Kocheon-Ri in the suburbs of Suweon city where were heavily damaged by this pest. Microorganisms were isolated from the both of diseased larvae and baiting method of soil microbes. In addition to, several species of the silkworm mucardine diseases were isolated for their infectivity of these fungi to the larvae of pine needle gall midge. Six species of fungi, Aureobasidium pullulans, Ascochyta sp, Verticillium psalliotae, Streptomyces sp., and two species of Aspergillus were isolated from the galls and soils, five species of muscardine diseases, Isaria farinosa, Spicaria pracina, Oospora destructor, Aspergillus flavus and A. oryzae were also identified from the silkworm corpse collected in the silkworm rearing farmers. Total of eleven species of fungi identified from the both of the larval of pine needle gall midge and silkworm larvae were tested for their pathogenecity to the larvae of pine needle gall midge. All of eleven species of fungi identified showed a considerable infectivity to the larvae. Twenty nine different kinds of bacteria were isolated from the both of diseased larvae and infested soils through the direct planting for the larvae and streaking for the corpse. The infectivity test is in progress. However two kinds of bacteria were recognized to be pathogenic to the larvae tested.

본시험은 솔잎혹파리의 미생물학적 방제대책의 일관으로서 미생물의 분리동정과 병원성을 연구 조사하고자 행하여졌다. 1. 솔잎혹파리의 유충에서 분리동정 된 균은 Verticillium psalliotae, Aureobdsidium pullulansa, Streptomyces sp., Aspergillus sp.(2종), Ascochyt, sp.의 6종이었으며 6종 전부가 병원성이 있었다. 2. 추기 양잠농가로부터 Spicaria pracina, Isaria farinosa, Oospora destructor, Aspergillus oryzae, A. flavus의 5종의 누에 경화병균을 분리 동정하여, 솔잎혹파리유충에 대한 병원성을 검정한 결과, 5종 전부가 강력한 병원성을 나타내었다. 3. 솔잎혹파리 유충에서는 29종의 colony가 나타났으며 그 중에서 Bacillus 계통이 11, coccus 계통이 9개로 구분되었으며, 현재 병원성검정은 진행 중에 있으나 No.4와 No.9-1균주는 병원성이 인정되었다.