• Title/Summary/Keyword: Bacteriology

Search Result 191, Processing Time 0.025 seconds

Suppression of Bacterial Wilt with Fuorescent Pseudomonads, TS3-7 strain (Fluorescent siderophore 생산균주, TS3-7에 의한 풋마름병 발병 억제)

  • Kim, Ji-Tae;Cho, Hong-Bum;Kim, Shin-Duk
    • Applied Biological Chemistry
    • /
    • v.48 no.3
    • /
    • pp.296-300
    • /
    • 2005
  • Among the root colonizing and plant growth promoting bacteria isolated from the bacterial wilt suppressive soil, five strains were detected to produce siderophores by CAS agar assay. The most effective isolate, TS3-7 strain induced significant suppression of bacterial wilt disease in tomato and pepper plants. Seed treatment followed by soil drench application with this strain resulted in over 80% reduction of bacterial wilt disease compared with the control. Significant disease suppression by TS3-7 strain was related to the production of siderophore. Besides iron competition, induction of resistance of the host plant with siderophore was suggested to be another mode of action that suppress bacterial wilt, based on the lack of direct antibiosis against pathogen in vitro. According to Bergey's Manual of Systemic Bacteriology and 16S rDNA sequence data, TS3-7 stain was identified as Pseudomonas sp. TS3-7.

Isolation and Screening of Pepsin Inhibitor-Producing Actinomycetes (Pepsin 저해물질을 생산하는 방선균의 분리 및 검색)

  • 박석규;성낙계;노종수
    • Microbiology and Biotechnology Letters
    • /
    • v.17 no.2
    • /
    • pp.115-120
    • /
    • 1989
  • For the purpose of obtaining microorganisms which produced an extracellular pepsin inhibitor, screening test was carried out. One strain of Actinomycetes (GF 155-2) isolated from soil samples showed a high inhibitory activity against porcine pepsin. The morphological, physiological and cultural characteristics of the strain GE 155-2 on various culture media were studied according to ISP methods and Bergey's manual of determinative bacteriology (8th ed.). This Actinomycetes GE 155-2 was found to be similar to the genus Microtetraspora.

  • PDF

Clinical Study of Empyema Thoracis (I) (농흉의 임상적 고찰 (제 1보))

  • 유회성
    • Journal of Chest Surgery
    • /
    • v.4 no.2
    • /
    • pp.95-100
    • /
    • 1971
  • The incidence of empyema has been drastically reduced with the advent of antimicrobial drugs,however,there is still complicated and difficult problems of management of empyema remaining. During the period of December, 1958 to December, 1962, 90 patients nf empyema thoracis were managed in the Department of Thoracic Surgery of the National Medical Center, and this series deals with the incidence,etiologic consideration, bacteriology and management of empyema with its result. 1] Male predominates with the ratio of 3.1: 1, and peak age incidence lies in 3rd decade. 2] Most common etiologic factor is bronchorespiratory infection among which tuberculosis remains highest incidence. 3] 56.7% of patients shows positive result of bacteriologic study and about half of positive culture series shows mixed infection or changing pattern of bacteriological strains during serial examinations. 4] Complete cure is obtained in 84.4% of patients with 5 deaths.

  • PDF

Mineral Water Investigation on 10 Area in Seoul (서울시내(市內) 10개(個) 약수(藥水)의 수질오염(水質汚染)에 관(關)한 연구(硏究))

  • Kim, Hyung-Suk;Koo, Do-Suh;Park, Yang-Won
    • Journal of Preventive Medicine and Public Health
    • /
    • v.10 no.1
    • /
    • pp.59-61
    • /
    • 1977
  • We are calling the erupting ground water as drugwater or mineral water in Korea and thinking those water is good for health from ancient. But most of them were used by mountainer as drinking water and food making water. Authors tried to investigate the evidence of contamination by user on the 10 erupting ground water and gained following results: 1. All of the mineral, waters on 10 area in Seoul were unfitable to drinking water standard. 2. In the view of the bacteriology 80% were contaminated by coliform group. 3. The highest value of the free carbon dioxide contents were 652.96 ppm at Sam Sun Mineral Water.

  • PDF

Microbial Genome Analysis and Application to Clinical Bateriology (미생물의 유전자(Genome) 해석과 임상세균학에 이용)

  • Kim, Sung-Kwang
    • Journal of Yeungnam Medical Science
    • /
    • v.19 no.1
    • /
    • pp.1-10
    • /
    • 2002
  • With the establishment of rapid sequence analysis of 16S rRNA and the recognition of its potential to determine the phylogenetic position of any prokaryotic organism, the role of 16S rRNA similarities in the present species definition in bacteriology need to be clarified. Comparative studies clearly reveal the limitations of the sequence analysis of this conserved gene and gene product in the determination of relationship at the pathogenic strain level for which DNA-DNA reassociation experiments still constitute the superior method. Since today the primary structure of 16S rRNA is easier to determine than hybridization between DNA strands, the strength of the sequence analysis is to recognize the level at which DNA pairing studies need to be performed, which certainly applies to similarities of 97% and higher.

  • PDF

VanB-vanA Incongruent VRE Isolated from Animals and Humans in 1999

  • Shin En-Joo;Hong Hyun-Gin;Ike Yasuyoshi;Lee Kyung-Won;Park Yong-Ho;Lee Dong-Taek;Lee Yeon-Hee
    • Journal of Microbiology
    • /
    • v.44 no.4
    • /
    • pp.453-456
    • /
    • 2006
  • 16 chicken isolates and four clinical isolates of VanB-vanA incongruent vancomycinresistant Enterococcus faecium strains without vanS were isolated in 1999. Pulsed-field gel electrophoresis revealed only a peripheral relationship between the chicken isolates and clinical isolates, but suggested clonal spread in the chicken isolates.

Studies on the Isolation and Identification of Genus Strptomyces (Streptomyces속의 분리 및 동정에 관한 연구)

  • 이민재;하영칠;안정선
    • Korean Journal of Microbiology
    • /
    • v.14 no.1
    • /
    • pp.25-35
    • /
    • 1976
  • A taxonomical sutdy was made on the Streptomyces species isolated from soils in this country, most of which were collected during the period from April, 1974 to July 1975. J.S.P. Methods (1966), I.S.P. Descriptions (1968-1972) and Bergy's Manual of Determinative Bacteriology (1974) were used for most of the experimental methods and identifications. As a result, 24 species were identified as follows ; S. albolongus, S galticri.S. Nashvillensis. S. showdoensis. S.norbonensis. S. flacocirens. S. resistomycificus. S> reshiriensis. S. chromofuscus. S. parvullus. S, chibaensis. S.canus. S. albulus. S. amlachiticus. S.griseoflavus. S. griscoincarnotus. .S. rubiginosus. S. bacillaris. S. setonill. S.intermedius. S. griseinus. S.subrutilus. S.reseosporus.

  • PDF

Characteristics of verotoxin non-producing Escherichia coli O157 and verotoxin-producing E coli isolated from healthy cattle (정상 소 분변에서 분리한 verotoxin을 산생하지 않는 Escherichia coli O157과 verotoxin을 산생하는 E coli의 특성 조사)

  • Jung, Byeong-yeal;Jung, Suk-chan;Park, Hong-je;Cho, Gil-jae;Kim, Bong-hwan
    • Korean Journal of Veterinary Research
    • /
    • v.40 no.3
    • /
    • pp.525-531
    • /
    • 2000
  • Verotoxin non-producing E coli O157 strains have been isolated from cattle feces and compared in particular regard to biochemical properties and genotypes with verotoxin-producing E coli (VTEC). E coli O157 : nonH7 strains had different phenotypes in sorbitol fermentation and ${\beta}-glucuronidase$ activity from E coli O157 : H7. Regardless of verotoxin production ability of E coli O157 : H7, uidA gene was uniquely detected from sorbitol and ${\beta}-glucuronidase$ negative E coli O157 : H7. Forty five fecal samples from 6 dairy farms were obtained and VTEC was detected as 15.6% (7 strains) of the samples. Most VTEC isolates were positive for sorbitol fermentation and ${\beta}-glucuronidase$ activity but negative for eaeA gene. This study suggested that cattle could be a reservior for VTEC. However, absence of eaeA gene in VTEC isolates from most of healthy cattle suggested that they might be less virulent than eaeA-positive E coli against human health.

  • PDF

Distribution of R Factors in Salmonella and Escherichia Coli Isolated from Korean Domestic Animals (우리나라 가축(家畜)에서 분리(分離)한 Salmonella 및 대장균(大腸菌)의 내성인자(耐性因子) R의 분포(分布))

  • Ha, Tai-You;Chung, Sun-Sik;Kang, Byung-Kyu
    • The Journal of the Korean Society for Microbiology
    • /
    • v.6 no.1
    • /
    • pp.21-27
    • /
    • 1971
  • Recent reports confirm that R factors is widespread in Korea among Enterobacteriaceae isolated from humans. However, no reports have been made concerning the incidence of transferable drug resistance in domestic animals in this country. A total of 211 isolates of Escherichia coli, including 94 strains from dogs, 76 strains from pigs, 30 strains from chickens, and 21 strains from cow milk, were examined for drug resistance and distribution of R factors. And, respective two strains of Salmonella E group and Salmonella cholerasuis which were isolated from dogs and pigs, respectively were also examined for the same purposes. Of 211 strains of E. coli isolated, 66.8% were found to be resistant to 8 antibacterial agents such as streptomycin(SM), tetracycline(TC), chloramphenicol(CP), ampicillin sodium(AP), nalidixic acid(NA), gentamicin(GM), and polymyxin B(PX). Among the isolates, 86.2% of the strains from dogs, 70% of the strains from chickens, 43.4% of the strains from pigs, and 28.6% of the strains from milk, respectively, were found to be resistant to the drugs. The following percentage of resistance of E. coli to each individual drugs was encountered: of 94 strains from dogs, AP, 64.9%; SM, 20.2%; NA, 12.8%, CP and PX, 8.5% each; GM, 2.1% each; GM, 2.1%. Among 76 strains from pigs, 42.2% and 2.6% each were resistant to TC, AP and PX, respectively. Among 30 strains from chickens, 43.3% were resistant to SM, TC, AP, respectively, and no strains were resistant to the other drugs. No strains of the isolated from milk were resistant to the drugs, except that 28.6% were resistant to SM and AP, respectively. Of the strains from dogs, multiply resistant strains(56.8%) were more than singly resistant one(43.2%) and sixteen different drug resistant patterns were observed. The most frequently encountered patterns were AP TC AP and SM CP AP NA. Of the isolates from other sources, the most frequently encountered resistant patterns were as follows: TC among the strains from pigs; SM TC AP from chickens; SM AP from milk. Of the resistant strains from dogs, 32% carried R factors and the most common resistance patterns of R factors were AP TC AP and SM TC CP, whereas 35.2% of the resistant strains from pigs carried R factors of which the most common encountered pattern was TC. Of the resistant strains from chickens, 46.9% carried R factors of which the most common patterns were SM TC TC AP and AP, whereas 50% of the resistant strains from milk carried R factors of which the most common pattern was SM. Of 4 strains of Salmonella isolated, no strains were resistant to the drugs, except that only one strain of Salmonella E group isolated from a dog was resistant to AP. The strain did not harbor R factor.

  • PDF