• 미생물학회지
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• 제39권1호
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• pp.40-44
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• 2003

탄저균은 그람양성 아포형성세균으로 탄저를 일으키는 원인균이다. Bacillus cereus그룹에 속하는 22종을 포함하여 Bacillus 속의 29종에서 탄저균을 검증할 수 있는 DNA 마커를 개발하고 이를 이용하여 B. cereus 그룹에서 탄저균만을 구분하였다. 한국산 탄저균 경주로부터 709 bp마커(KHTS)를 확보하였다. KHTS분절로부터 얻어진 internal primer set의 PCR 산물은 B. cereus 그룹의 다른 종으로부터 탄저균만을 구별하였다.

• 미생물학회지
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• 제37권1호
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• pp.56-60
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• 2001

탄저균의 분자적 다양성 분석은 다양한 DNA표지의 부족으로 쉬운 일이 아니어서, 본 연구에서는 random amplified polymorphic DNA (RAPD)-PCR을 이용하여 Bacillus 속으로부터 탄저균을 구별할 수 있는 새로운 DNA 표지를 개발하고자 하였다. RAPD-PCR을 이용한 분석은 다양한 Bacillus 종으로부터 탄저균을 동정할 수 있었으며, 아울러 Bacillus 종 사이에서 확실한 유전적인 변이를 확인할 수 있었다. 이러한 분석은 간단, 신속하고, 그리고 정확하게 탄저균을 진단하는데 활용할 수 있다고 본다.

• 미생물학회지
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• 제44권4호
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• pp.333-338
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• 2008

Bacillus anthracis, B. cereus, B. thuringiensis 를 분류하기 위해 rpoB 유전자 배열을 이용한 컴퓨터 분석 작업을 수행하였다. 17개의 B. anthracis, 9개의 B. cereus, 7개의 B. thuringiensis 를 database에서 구하였다. B. anthracis 는 rpoB 유전자의 in silico 제한효소 절단에 의해, B. cereus, B. thuringiensis 2 group과 구별되었다. 그러나 B. cereus와 B. thuringiensis 는 제한효소 절단에 의해 구분되지는 않고, 염기배열과 Blast 탐색의 도움으로 구분이 가능하였다. 본 연구를 통해 3 종류의 Bacillus 종을 동정할 수 있는 알고리즘이 개발되었다.

• 한국군사과학기술학회지
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• 제7권1호
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• pp.77-81
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• 2004

The etiological agent is Bacillus anthracis, a gram-positive rod-shaped bacterium able to form spores. In order to elucidate the mechanism of infecttion on human macrophage cells, we performed two-dimensional electrophoresis and MALDI-TOF analysis using the infected human macrophage cells with the spores of B. anthracis Sterne of inactivated B. anthracis Sterne. We identified 9 proteins which related to the infection of Bacillus anthracis spores on human macrophage cells at the early stage events. Maybe nine proteins will be bio-markers and vaccine candidates to the Bacillus anthracis spore infection.

• Journal of Microbiology and Biotechnology
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• 제19권11호
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• pp.1475-1481
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• 2009

Anthrax is a zoonotic disease caused by Bacillus anthracis, and well recognized as a potential agent for bioterrorism. B. anthracis can be identified by detecting the virulence factors genes located on two plasmids, pXO1 and pXO2. The aim of the present study was to determine the presence of virulence genes in 27 isolates of B. anthracis isolated from clinical and environmental samples. For this purpose, multiplex PCR and DNA probes were designed to detect protective antigen (pag), edema factor (cya), lethal factor (lef), and capsule (cap) genes. Our results indicated that all the isolates contained all the above virulence genes, suggesting that the isolates were virulent. To the best our knowledge, this is the first study about the determination of virulence marker genes in clinical and environmental isolates of B. anthracis using multiplex PCR and DNA probes in India. We suggest that the above methods can be useful in specific identification of virulent B. anthracis in clinical and environmental samples.

• 대한미생물학회지
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• 제35권1호
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• pp.31-40
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• 2000

Bacillus anthracis, the etiological agent of anthrax has been classified into the Bacillus subgroup I with B. cereus, B. mycoides and B. thuringiensis based on morphological and DNA similarity. DNA studies have further indicated that these species have very AT-rich genomes and high homology, indeed it has been proposed that these four sub-species be recognized as members of the one species. Several methods have been developed to obtain good differentiation between these species. However, none of these methods provides the means for an absolutely correct differntiation. The analysis of fatty acid methyl esters (FAMEs) was employed as a quick, simple and reliable method for the identification of 21 B. anthracis strains and closley related strains. The most significant differences were found between B. anthracis and B. anthracis closely related strains in FAMEs profiles. All tested strains of B. anthracis had a branched fatty acid C17:1 Anteiso A, whereas the fraction of unsaturated fatty acid Iso C17:1 w10c was found in B. anthracis closely related strains. By UPGMA clustering analysis of FAMEs profiles, all of the tested strains were classified into two clusters defined at Euclidian distance value of 24.5. The tested strains of B. anthracis were clustered together including Bacillus sp. Kyungjoo 3. However, the isolates of B. anthracis closely related spp. Rho, S10A, 11R1, CAU9910, CAU9911, CAU9912 and CAU9913 were clustered with the other group. On the basis of these results, isolates of B. anthracis Bongchon, Kyungjoo 1, 2 and Bacillus sp. Kyungjoo 3 were reclassified as a B. anthracis. It is concluded that FAMEs analysis provides a sensitive and reliable method for the identification of B. anthracis from closely related taxa.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.806-811
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• 2007

Bacillus anthracis is a soil pathogen capable of causing anthrax that is closely related to several environmental species, including B. cereus, B. mycoides, and B. thuringiensis. DNA homology studies showed that B. anthracis, B. cereus, B. mycoides, and B. thuringiensis are closely related, with a high sequence homology. To establish a method to specifically detect B. anthracis in situations such as environmental contamination, we initially performed RAPD-PCR with a 10-mer random primer and confirmed the presence of specific PCR bands only in B. anthracis species. One region specific for B. anthracis was cloned and sequenced, and an internal primer set was designed to amplify a 241-bp DNA fragment within the sequenced region. The PCR system involving these specific primer sets has practical applications. Using lyses methods to prepare the samples for PCR, it was possible to quickly amplify the 241-bp DNA segment from samples containing only a few bacteria. Thus, the PCR detection method developed in this study is expected to facilitate the monitoring of environmental B. anthracis contamination.

• Journal of Microbiology and Biotechnology
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• 제23권6호
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• pp.750-758
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• 2013

Anthrax is a bacterial disease caused by the aerobic spore-forming bacterium Bacillus anthracis, which is an important pathogen owing to its ability to be used as a terror agent. B. anthracis spores can escape phagocytosis and initiate the germination process even in antimicrobial conditions, such as oxidative stress. To analyze the oxidative stress response in B. anthracis and thereby learn how to prevent antimicrobial resistance, we performed protein expression profiling of B. anthracis strain HY1 treated with 0.3 mM hydrogen peroxide using a comparative proteomics-based approach. The results showed a total of 60 differentially expressed proteins; among them, 17 showed differences in expression over time. We observed time-dependent changes in the production of metabolic and repair/protection signaling proteins. These results will be useful for uncovering the metabolic pathways and protection mechanisms of the oxidative response in B. anthracis.

• 한국군사과학기술학회지
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• 제14권2호
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• pp.305-312
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• 2011

Bacillus anthracis(Ba) is a Gram-positive spore-forming bacterium that causes the disease anthrax. The feature of Ba is the presence of two large virulence plasmids, pXO1 and pXO2. Molecular genotyping of Ba has been difficult to the lack of polymorphic DNA marker. Ba isolated from Korea has been genotyped using various nucleotide analysis methods, such as 16s rDNA sequencing and multiple-locus variable-number tandem repeat (MLVA) analysis. We identified genotypes that represent a genetic lineage in the B1 cluster. This study emphasized the need to perform molecular genotyping when attempting to verify a strain-specific Ba.

• 미생물학회지
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• 제37권1호
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• pp.49-55
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• 2001

탄저(anthrax)는 그람양성이고 포자형성 세균인 탄저균(Bacillus anthracis)으로부터 발명되어진다. 탄저독소는 세가지 요소로 구성되어 있으며, 방어항원(PA)은 숙주세포 표면에서 탄저 독소단백질 및 이종단백질을 세포질 내로 이동시키는 역할을 한다. 본 연구에서는 PA의 분자적 다양성과 국내에서 탄저균의 진화를 이해하고 확인하기 위해 국내외에서 발견된 탄저균 4 균주와 기존에 보고된 탄저균 26 균주로부터 2,294 bp의 PA유전자(pagA)의 DNA 염기서열을 분식하였다. 탄저균 30 균주으로부터 PA유전자의 염기서열을 비교 분식한 결과, 8개 부위에서 돌연변이를 확인 하였다. 돌연변이가 일어난 부위에 따라서 탄저균을 10종류의 PA 유전자형과 4 종류의 PA 표현형으로 구분하였다. 한국 경주에서 분류된 B. anthracis BAK는 600번째 아미노산 alanine이 valine으로 바뀌어서 B. anthracis ATCC 14185 보다 LF와 PA의 결합 위치를 근접하게 하였다. 탄저균의 Pag의 염기서열을 통한 계통분석학적인 분석 결과는 염색체상에서의 분류와 일치하여 탄저균사이에서 pXO1 플라스미드의 수평적인 이동은 없는 것으로 사료된다.