Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Random Amplified Polymorphic DNA-PCR Analysis for Identification of Bacillus anthracis

탄저균의 Random Amplified Polymorphic DNA-PCR 분석

  • 김성주 (한양대학교 생화학 및 분자생물학과) ;
  • 박경현 (한양대학교 생화학 및 분자생물학과) ;
  • 김형태 (한양대학교 생화학 및 분자생물학과) ;
  • 조기승 (한양대학교 생화학 및 분자생물학과) ;
  • 김기천 (한양대학교 생화학 및 분자생물학과) ;
  • 최영길 (한양대학교 생물학과) ;
  • 박승환 (생명공학연구원) ;
  • 이남택 (육군사관학교 화학과) ;
  • 채영규 (한양대학교 생화학 및 분자생물학과)
  • Published : 2001.03.01
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Abstract

Molecular typing of Bacillus anthracis has been extremely difficult due to the lack of polymorphic DNA markers. Aiming to develop a DNA marker specific for Bacillus anthracis and to be able to discriminate this species from Bacillus genus, we applied the random amplified polymorphic DNA (RAPD)-PCR. We have identified B. anthracis from various Bacillus species. The analysis performed by RAPD clearly demonstrated substantial genetic variations among Bacillus species. This type of analysis is an easy, quick and highly discriminatory technique that may help in diagnosis of anthrax.

탄저균의 분자적 다양성 분석은 다양한 DNA표지의 부족으로 쉬운 일이 아니어서, 본 연구에서는 random amplified polymorphic DNA (RAPD)-PCR을 이용하여 Bacillus 속으로부터 탄저균을 구별할 수 있는 새로운 DNA 표지를 개발하고자 하였다. RAPD-PCR을 이용한 분석은 다양한 Bacillus 종으로부터 탄저균을 동정할 수 있었으며, 아울러 Bacillus 종 사이에서 확실한 유전적인 변이를 확인할 수 있었다. 이러한 분석은 간단, 신속하고, 그리고 정확하게 탄저균을 진단하는데 활용할 수 있다고 본다.

Keywords

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