Journal of the Korea Institute of Military Science and Technology (한국군사과학기술학회지)

The Korea Institute of Military Science and Technology (한국군사과학기술학회)
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Strain-specific Detection of Bacillus Anthracis using Multiple-locus Variable-number Tandem Repeat Analysis

Multiple-locus Variable-number Tandem Repeat 분석을 사용한 Bacillus Anthracis 균주간 특이성 규명

  • Received : 2011.01.15
  • Accepted : 2011.03.25
  • Published : 2011.04.05
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Abstract

Bacillus anthracis(Ba) is a Gram-positive spore-forming bacterium that causes the disease anthrax. The feature of Ba is the presence of two large virulence plasmids, pXO1 and pXO2. Molecular genotyping of Ba has been difficult to the lack of polymorphic DNA marker. Ba isolated from Korea has been genotyped using various nucleotide analysis methods, such as 16s rDNA sequencing and multiple-locus variable-number tandem repeat (MLVA) analysis. We identified genotypes that represent a genetic lineage in the B1 cluster. This study emphasized the need to perform molecular genotyping when attempting to verify a strain-specific Ba.

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References

  1. Mock M., Fouet A., Anthrax, Annu Rev Microbiol, 55 : 647-671., 2001. https://doi.org/10.1146/annurev.micro.55.1.647
  2. Turnbull P. C., Lindeque P. M., Le Roux J., Bennett A. M., Parks S. R., Airborne Movement of Anthrax Spores from Carcass Sites in the Etosha National Park, Namibia, J Appl Microbiol, 84(4) : 667-676, 1998. https://doi.org/10.1046/j.1365-2672.1998.00394.x
  3. Daffonchio D., Borin S., Frova G., Gallo R., Mori E., Fani R., Sorlini C., A Randomly Amplified Polymorphic DNA Marker Specific for the Bacillus Cereus Group is Diagnostic for Bacillus Anthracis, Appl Environ Microbiol, 65(3) : 1298-1303, 1999.
  4. Patra G., Sylvestre P., Ramisse V., Therasse J., Guesdon J. L., Isolation of a Specific Chromosomic DNA Sequence of Bacillus Anthracis and its Possible Use in Diagnosis, FEMS Immunol Med Microbiol, 15(4) : 223-231, 1996. https://doi.org/10.1111/j.1574-695X.1996.tb00088.x
  5. Patra G., Vaissaire J., Weber-Levy M., Le Doujet C., Mock M., Molecular Characterization of Bacillus Strains Involved in Outbreaks of Anthrax in France in 1997, J Clin Microbiol, 36(11) : 3412-3414, 1998.
  6. Gierczynski R., Jakubczak A., Jagielski M., Extended Multiple-locus Variable-number Tandem-repeat Analysis of Bacillus Anthracis Strains Isolated in Poland, Pol J Microbiol, 58(1) : 3-7, 2009.
  7. Keim P., Kalif A., Schupp J., Hill K., Travis S. E., Richmond K., Adair D. M., Hugh-Jones M., Kuske C. R., Jackson P., Molecular Evolution and Diversity in Bacillus Anthracis as Detected by Amplified Fragment Length Polymorphism Markers, J Bacteriol, 179(3) : 818-824, 1997. https://doi.org/10.1128/jb.179.3.818-824.1997
  8. Price L. B., Hugh-Jones M., Jackson P. J., Keim P., Genetic Diversity in the Protective Antigen Gene of Bacillus Anthracis, J Bacteriol, 181(8) : 2358-2362, 1999.
  9. Andersen G. L., Simchock J. M., Wilson K. H., Identification of a Region of Genetic Variability Among Bacillus Anthracis Strains and Related Species, J Bacteriol, 178(2) : 377-384, 1996. https://doi.org/10.1128/jb.178.2.377-384.1996
  10. Henderson I., Yu D., Turnbull P. C., Differentiation of Bacillus Anthracis and other 'Bacillus Cereus Group' Bacteria using IS231-derived Sequences, FEMS Microbiol Lett, 128(2) : 113-118, 1995. https://doi.org/10.1111/j.1574-6968.1995.tb07509.x
  11. Jackson P. J., Walthers E. A., Kalif A. S., Richmond K. L., Adair D. M., Hill K. K., Kuske C. R., Andersen G. L., Wilson K. H., Hugh-Jones M. et al, Characterization of the Variable-number Tandem Repeats in vrrA from Different Bacillus Anthracis Isolates, Appl Environ Microbiol, 63(4): 1400-1405, 1997.
  12. Van Ert M. N., Easterday W. R., Huynh L. Y., Okinaka R. T., Hugh-Jones M. E., Ravel J., Zanecki S. R., Pearson T., Simonson T. S., U'Ren J. M. et al, Global Genetic Population Structure of Bacillus Anthracis, PLoS One, 2(5) : e461, 2007. https://doi.org/10.1371/journal.pone.0000461
  13. Fasanella A., Losito S., Adone R., Ciuchini F., Trotta T., Altamura S. A., Chiocco D., Ippolito G., PCR Assay to Detect Bacillus Anthracis Spores in Heat-treated Specimens, J Clin Microbiol, 41(2) : 896-899, 2003. https://doi.org/10.1128/JCM.41.2.896-899.2003
  14. Keim P., Price L. B., Klevytska A. M., Smith K. L., Schupp J. M., Okinaka R., Jackson P. J., Hugh- Jones M. E., Multiple-locus Variable-number Tandem Repeat Analysis Reveals Genetic Relationships within Bacillus Anthracis, J Bacteriol, 182(10) : 2928-2936, 2000. https://doi.org/10.1128/JB.182.10.2928-2936.2000
  15. Kim T. H., Seo G. M., Jung K. H., Kim S. J., Kim J. C., Oh K. G., Koo B. S., Chai Y. G., Generation of a Specific Marker to Discriminate Gacillus Anthracis from Other Bacteria of the Bacillus Cereus Group, J Microbiol Biotechnol, 17(5) : 806-811, 2007.
  16. Marston C. K., Gee J. E., Popovic T., Hoffmaster A. R., Molecular Approaches to Identify and Differentiate Bacillus Anthracis from Phenotypically Similar Bacillus Species Isolates, BMC Microbiol, 6 : 22, 2006. https://doi.org/10.1186/1471-2180-6-22
  17. Tsygankova O. I., Eremenko E. I., Riazanova A. G., Tsygankova E. A., Multiplex Amplification Test System for the Identification and Differentiation of Bacillus Anthracis, Zh Mikrobiol Epidemiol Immunobiol, (3) : 69-74, 2005.
  18. Keim P., Van Ert M. N., Pearson T., Vogler A. J., Huynh L. Y., Wagner D. M., Anthrax Molecular Epidemiology and Forensics : Using the Appropriate Marker for Different Evolutionary Scales, Infect Genet Evol, 4(3) : 205-213, 2004. https://doi.org/10.1016/j.meegid.2004.02.005
  19. Miettinen M. K., Siitonen A., Heiskanen P., Haajanen H., Bjorkroth K. J., Korkeala H. J., Molecular Epidemiology of an Outbreak of Febrile Gastroenteritis Caused by Listeria Monocytogenes in Cold-smoked Rainbow Trout, J Clin Microbiol, 37(7) : 2358-2360, 1999.