• BMB Reports
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• v.40 no.1
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• pp.44-52
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• 2007

Kinesins, as a kind of microtubule-based motor proteins, have a conserved microtubule-binding site in their motor domain. Here we report that two homologous kinesins in Arabidopsis thaliana, KatB and KatC, contain a second microtubule-binding site in their tail domains. The prokaryotic-expressed N-terminal tail domain of the KatC heavy chain can bind to microtubules in an ATP-insensitive manner. To identify the precise region responsible for the binding, a serious of truncated KatC cDNAs encoding KatC N-terminal regions in different lengths, KatC1-128, KatC1-86, KatC1-73 and KatC1-63, fused to Histidine-tags, were expressed in E. coli and affinity-purified. Microtubule cosedimentation assays show that the site at amino acid residues 74-86 in KatC is important for microtubule-binding. By similarity, we obtained three different lengths of KatB N-terminal regions, KatB1-384, KatB1-77, and KatB1-63, and analyzed their microtubule-binding ability. Cosedimentation assays indicate that the KatB tail domain can also bind to microtubules at the same site as and in a similar manner to KatC. Fluorescence microscopic observations show that the microtubule-binding site at the tail domain of KatB or KatC can induce microtubules bundling only when the stalk domain is present. Through pull-down assays, we show that KatB1-385 and KatC1-394 are able to interact specifically with themselves and with each other in vitro. These findings are significant for identifying a previously uncharacterized microtubule-binding site in the two kinesin proteins, KatB and KatC, and the functional relations between them.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.1A
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• pp.25-33
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• 2003

We derive the outage probabilities for the Erlang capacity and link margin based on handoffs in the reverse link of a CDMA system of 14.4 kbps/1.9 GHz band, calculate them with the same parameters, and then with the maximum realizable Erlang capacity analyze newly the cell coverage in different areas by the COST-231 model If it decreases from 5 % to 2 % in case of $E_b/N_0$=5 dB under a soft handoff, the result shows a very reliable link with additional increment of 0.88 dB in the margin and a high quality of service within the coverage decrease of 0.12 km, 0.25 km, and 1 km in a city, suburban, and open areas respectively on the decrease of 2 Erlang in the capacity. When the $E_b/N_0$ decreases from 7 dB to 5 dB within the outage probability of 5 %, it shows the increase of 9 Erlang and of 0.2 km in a city on the same margin of 2.8 dB. Then, comparing it with a single cell, it shows the decrease of more than 10 Erlang in the capacity, but the enlargement of 0.17 km, 0.3 km, and 1 km in them on the margin decrease of 1.31 dB, also, with a hard handoff, the soft handoff gain of the increase of 10 Erlang and of 0.22 km, 0.5 km, and 2 km on the decrease of 2.16 dB.

• Journal of Korean Institute of Industrial Engineers
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• v.28 no.1
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• pp.36-43
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• 2002

We analyze bulk service $M/G^{b}/1$ queues using the arrival time approach of Chae et al. (2001). As a result, the decomposition property of the M/G/1 queue with generalized vacations is extended to the $M/G^{b}/1$ queue in which the batch size is exactly a constant b. We also demonstrate that the arrival time approach is useful for relating the time-average queue length PGF to that of the departure time, both for the $M/G^{b}/1$queue in which the batch size is as big as possible but up to the maximum of constant b. The case that the batch size is a random variable is also briefly mentioned.

• Bulletin of the Korean Mathematical Society
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• v.54 no.1
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• pp.343-358
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• 2017

Let {$X_i$} be a sequence of stationary ${\alpha}-mixing$ random variables with probability density function f(x). The recursive kernel estimators of f(x) are defined by $$\hat{f}_n(x)={\frac{1}{n\sqrt{b_n}}{\sum_{j=1}^{n}}b_j{^{-\frac{1}{2}}K(\frac{x-X_j}{b_j})\;and\;{\tilde{f}}_n(x)={\frac{1}{n}}{\sum_{j=1}^{n}}{\frac{1}{b_j}}K(\frac{x-X_j}{b_j})$$, where 0 < $b_n{\rightarrow}0$ is bandwith and K is some kernel function. Under appropriate conditions, we establish the Berry-Esseen bounds for these estimators of f(x), which show the convergence rates of asymptotic normality of the estimators.

• Proceedings of the Korea Electromagnetic Engineering Society Conference
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• 2000.11a
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• pp.146-149
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• 2000

SiGe HUT뜰 이용하여 Cellular 주파수 대역(824-849 MHz)에서 MMIC 상향 주파수 혼합기와 가변이득 증폭기를 설계하였다. 동작 전압은 2.7 V 이며, 이중평형 구조의 상향 주파수 혼합기는 12 dB의 변환이득, -0.6 dBm의 1dB 이득압축 출력전력, 30 dB 이상의 LO-RF 단자 격리도 특성, 1.25의 LO-VSWR. 1.34의 RF-VSWR을 가지며, 상호컨덕턴스형 가변이득 증폭기는 35 dB의 최대 선형이득, 13 dBm의 1dB 이득압축 출력전력, 42dB의 가변이득, 23dB의 3차 상호변조 교점 출력전력(OIP$_3$), 1.27의 입력 VSWR, 1.1의 출력 VSWR 특성을 보인다.

• Journal of Microbiology and Biotechnology
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• v.12 no.4
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• pp.679-682
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• 2002

The alkaloids, piperlongumine, piperine, pipernonaline, and piperoctadecalidine, isolated from Piper longum L., were found to inhibit the biosynthesis of aflatoxin $B_1$ (AF$B_1$) in Aspergillus flavus WRRC 3-90-42-12. Piperlongumine was the most active among the compounds tested, with a 96% inhibition of AF$B_1$biosynthesis at 0.2% (w/v) supplement in a potato dextrose agar (PDA) medium. The three other piperidine alkaloids, pipeline, pipernonaline, and piperoctadecalidine, also inhibited the biosynthesis of AF$B_1$. Of these three alkaloids, piperoctadecalidine exhibited a potent inhibitory activity with a 100% inhibition of AF$B_1$ production at 0.7% (w/v) supplement in a PDA medium. Therefore, piperlongumine and piperoctadecalidine could be used as antiaflatoxigenic agents in agricultural industries. To determine the antiaflatoxigenic mode of action of piperlongumine, further studies are needed.

• Journal of Surface Science and Engineering
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• v.27 no.4
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• pp.215-222
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• 1994

The role dispersoids has been studied in a number of researches as a key point for the high strength application of dispersion strengthened aluminum alloy. The mechanical alloying(MA) process with high mechanical properties of dispersion strengthened MA Al-8Ti-1B alloys were invested in order to evaluate their stress corrosion cracking(SCC) application. SCC properties of the mechanically alloyed Al-8Ti-1B were studied using slow strain rate test(SSRT). In this study Al-8Ti-1B alloy were more susceptible to SCC in solutions of pH=2.01 and 13.2 than pH=6.81 solution. In this study Al-8Ti-1B alloys by MA had more SCC resistance than Al-8Ti alloys or Al 7075-T73 alloys. So Al-8Ti-1B alloys by MA had more resistance in SSRT SCC susceptinility test than any other above alloying metals.

• Journal of the Korean Mathematical Society
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• v.42 no.1
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• pp.111-127
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• 2005

Let B and S be the unit ball and the unit sphere in $\mathbb{C}^n$, respectively. Let ${\sigma}$ be the normalized Lebesgue measure on S. Define the Privalov spaces $N^P(B)\;(1\;<\;p\;<\;{\infty})$ by $$N^P(B)\;=\;\{\;f\;{\in}\;H(B) : \sup_{0 where H(B) is the space of all holomorphic functions in B. Let ${\varphi}$ be a holomorphic self-map of B. Let ${\mu}$ denote the pull-back measure ${\sigma}o({\varphi}^{\ast})^{-1}$. In this paper, we prove that the composition operator $C_{\varphi}$ is metrically bounded on $N^P$(B) if and only if ${\mu}(S(\zeta,\delta)){\le}C{\delta}^n$ for some constant C and $C_{\varphi}$ is metrically compact on $N^P(B)$ if and only if ${\mu}(S(\zeta,\delta))=o({\delta}^n)$ as ${\delta}\;{\downarrow}\;0$ uniformly in ${\zeta}\;\in\;S. Our results are an analogous results for Mac Cluer's Carleson-measure criterion for the boundedness or compactness of $C_{\varphi}$ on the Hardy spaces $H^P(B)$.

• Phonetics and Speech Sciences
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• v.7 no.1
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• pp.21-26
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• 2015

The aim of this study is to synthesize pathological breathy voice and to make a cepstral peak prominence (CPP) table following breathiness ranks by cepstral analysis to supplement reliability of the perceptual auditory judgment task. KlattGrid synthesizer included in Praat was used. Synthesis parameters consist of two groups, i.e., constants and variables. Constant parameters are pitch, amplitude, flutter, open phase, oral formant and bandwidth. Variable parameters are breathiness (BR), aspiration amplitude (AH), and spectral tilt (TL). Five hundred sixty samples of synthetic breathy vowel /a/ for male were created. Three raters participated in ranking of the breathiness. 217 were proved to be inadequate samples from perceptual judgment and cepstral analysis. Finally, 343 samples were selected. These CPP values and other related parameters from cepstral analysis are classified under four breathiness ranks (B0~B3). The mean and standard deviation of CPP is $16.10{\pm}1.15$ dB(B0), $13.68{\pm}1.34$ dB(B1), $10.97{\pm}1.41$ dB(B2), and $3.03{\pm}4.07$ dB(B3). The value of CPP decreases toward the severe group of breathiness because there is a lot of noise and a small quantity of harmonics.

• Transactions of the Korean Society of Pressure Vessels and Piping
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• v.18 no.1
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• pp.1-10
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• 2022

Currently, there is no technical standard and regulation for seismic analysis of non-nuclear safety piping. Accordingly, ASME Sec.III ND, a standards applied to safety class 3 piping, is applied. However, the technical standard applied for other than seismic analysis is ASME B31, which leads to controversy. In this study, the feasibility of applying ASME B31E was confirmed by reviewing rulescomparing technical standards, and evaluating piping allowable stress margins. The evaluation revealed that applying ASME B31.1 as a technical standard is too conservative compared to ASME Sec.III ND. On the other hand, ASME B31E (issued at the request of the industry) clearly presents the technical standards for seismic analysis of ASME B31 piping, and shows a similar level of conservatism compared to ASME Sec.III ND. It is expected to reduce the controversy over technical standards for seismic analysis of non-nuclear safety piping by applying ASME B31E.