• Journal of Microbiology and Biotechnology
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• v.23 no.2
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• pp.167-176
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• 2013

A novel bioactive molecule produced by Bacillus thuringiensis subsp. kurstaki Bn1 (Bt-Bn1), isolated from a common pest of hazelnut, Balaninus nucum L. (Coleoptera: Curculionidae), was determined, purified, and characterized in this study. The Bt-Bn1 strain was investigated for antibacterial activity with an agar spot assay and well diffusion assay against B. cereus, B. weinhenstephenensis, L. monocytogenes, P. savastanoi, P. syringae, P. lemoignei, and many other B. thuringiensis strains. The production of bioactive molecule was determined at the early logarithmic phase in the growth cycle of strain Bt-Bn1 and its production continued until the beginning of the stationary phase. The mode of action of this molecule displayed bacteriocidal or bacteriolytic effect depending on the concentration. The bioactive molecule was purified 78-fold from the bacteria supernatant with ammonium sulfate precipitation, dialysis, ultrafiltration, gel filtration chromatography, and HPLC, respectively. The molecular mass of this molecule was estimated via SDS-PAGE and confirmed by the ESI-TOFMS as 3,139 Da. The bioactive molecule was also determined to be a heat-stable, pH-stable (range 6-8), and proteinase K sensitive antibacterial peptide, similar to bacteriocins. Based on all characteristics determined in this study, the purified bacteriocin was named as thuricin Bn1 because of the similarities to the previously identified thuricin-like bacteriocin produced by the various B. thuringiensis strains. Plasmid elution studies showed that gene responsible for the production of thuricin Bn1 is located on the chromosome of Bt-Bn1. Therefore, it is a novel bacteriocin and the first recorded one produced by an insect originated bacterium. It has potential usage for the control of many different pathogenic and spoilage bacteria in the food industry, agriculture, and various other areas.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.17 no.4
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• pp.104-111
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• 2008

The objective of this study is to evaluate micro end-milling characteristics and tool wear behavior of AlN-hBN composites. First, AlN based composites with hBN contents in the range of 10 to 20vol% were prepared by hot-pressing. Vickers hardness and flexural strength of the prepared composite specimens were measured and compared according to the vol% of hBN variations. Then, cutting force variations were measured and analyzed using a tool dynamometer during the micro end-milling experiments; and machined surface shapes and roughness were investigated using a 3D non-contact type surface profiler. After micro end-milling, worn tools were investigated using a tool microscope and SEM images. From the experimental results, it can be observed that the cutting forces decreased, and surface qualities were improved with increasing hBN contents. At low content of hBN, tool chipping was observed; and tool wear rate decreased with increasing hBN contents. The results of this study insist that proper machining conditions, including tool wear behavior investigation, should be determined for the micro end-milling of AlN-hBN composites for its further application.

• Tribology and Lubricants
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• v.37 no.4
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• pp.125-128
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• 2021

A means of enhancing the performance of triboelectric nanogenerators (TENGs) is increasing the differences in work functions between contacting materials. Hexagonal boron nitride (h-BN) exhibits excellent mechanical properties and high chemical stability as well as a high work function. As a result, engineers in the field of energy harvesting have envisioned using h-BN in the electrification layer in TENGs. For the industrial application of h-BN in TENGs, large-scale production is necessary, and h-BN is generally exfoliated and dispersed in various solvents. In this study, we evaluate the performance of a TENG with h-BN nanoflakes in the polyimide (PI) layer. To synthesize a PI composite containing h-BN nanoflakes, h-BN powders are exfoliated and dispersed in poly(amic acid) (PAA), which is the precursor of PI. Then, h-BN dispersion is spin-coated onto the PI film and cured for 2 h under 300℃. This composite material can then be used for the electrification layer in TENGs. Below the electrification layer, an aluminum foil is placed and used as an electrode. When the contact and separation processes with polyethylene terephthalate are repeated, the fabricated TENG shows a maximum power density of 190.8 W/m². This study shows that h-BN is a promising material for enhancing the performance of the electrification layer in TENGs.

• The Korean Journal of Ceramics
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• v.1 no.3
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• pp.155-159
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• 1995

Borosilicate, $B_2O_3$ and BN derived from liquid precursors have been tested as shielding materials for the long period of oxidation resistance of C/BN composites at $650^{\circ}C$. Borosilicate coating displayed excellent oxidation resistance and low moisture absorbance, while $B_2O_3$ and BN were less effective in elevating the oxidation resistance. The enhancement of the oxidation resistance was explained as self-healing effect by viscous flow of the borosilicate glass over Tg, resulting in the reduction of the exposed carbon fibers in a BN matrix.

• Journal of the Korean Society for Heat Treatment
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• v.5 no.3
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• pp.157-164
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• 1992

Barkhausen noise (BN) is created by the abrupt changes in the magnetization of materials under applied AC magnetizing field. These changes are known to be sensitive to residual and applied stresses. In this study, BN theory was reviewed and it was examined how BN intensity was affected by simultaneous stress, hardness and microstructural changes. Also, magnetoelastic effect was used to evaluate residual stresses through carrying out the cantilever beam test. An increase in BN intensity was observed when applied and residual stress changes from compression into tension. Microstructural softening by tempering also increased the amount of BN. Therefore, the quantitative evaluation of residual stress and microstructural changes will be possible, provided BN method is more studied about various materials through comparing with different stress measuring techniques.

• Proceedings of the KWS Conference
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• 2007.11a
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• pp.248-250
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• 2007

이 연구는 cBN과 활성 Ag계 금속필러메탈을 이용하여 접합계면에서의 금속성분과 화합물, 탄화물의 거동을 분석하는데 있다. 진공 브레이징은 $900{\sim}1000^{\circ}C$에서 $25^{\circ}C$ 간격으로, 유지 시간은 각각 5, 10, 15, 20분으로 실시하였다. Ag-Cu-Ti을 사용하여 브레이징된 cBN은 $950{\sim}1000$도, 유지시간 10분 사이에서 각각 건전한 계면과 표면을 얻을 수 있었으며, 계면에서 Ti-rich상과 화합물이 확인되었다. 이상의 결과로 부터 화합물의 생성과 건전한 접합공정은 브레이징 온도와 시간이 좌우하며, N과 B, Ti의 함유량이 cBN의 브레이징 접합 특성의 중요변수로 생각되어진다. cBN과 Ag계 브레이징 필러의 계면에서의 미세조직 및 화학반응의 메커니즘은 DTA, SEM, EPMA, XRD를 이용하여 분석하였다.

• Journal of the Korean Ceramic Society
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• v.26 no.2
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• pp.179-186
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• 1989

The preparation of the fine BN powders by ammonia-nitridation of ammonia-decaborane derivate was attempted at temperature between 300 to 150$0^{\circ}C$. The formation mechanism of BN was examined and the resulant BN powder was characterized by means of IR, XRD, SEM and PSA method. In the nitridation below 80$0^{\circ}C$ bonding materials were identified with mainly BH and NH but above 80$0^{\circ}C$ with BN by IR spectra and X-ray patterns. Crystalite size, lattice constant and particle size distribution of hexagonal BN prepared at 1, 50$0^{\circ}C$ were La=470$\AA$, Lc=180$\AA$, a=2.5062$\pm$1$\AA$, c=6.8285$\pm$2$\AA$ and 2.0-5.4${\mu}{\textrm}{m}$, respectively.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.1
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• pp.89-93
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• 2012

c-BN films were deposited on SKH-51 steels by electron assisted hot filament CVD method and microstructure development was studied processing parameters such as bias voltage, temperature, etching and phase transformation at boundary layer between BN compound and steel to develop a high performance wear resistance tools. A negative bias voltage higher than 200V at substrate temperature of $800^{\circ}C$ and gas pressure of 20 torr in B2H6-NH3-H2 gas system was one of optimum conditions to produce c-BN films on the SKH-51 steels. Thin layer of hexagonal boron nitride phase was observed at the interface between c-BN layer and substrate.

• Journal of the Korean Society for Heat Treatment
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• v.21 no.4
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• pp.199-204
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• 2008

The destructive method is reliable and widely used for evaluating the properties of material but it is time-consuming and difficult to prepare specimens from in-service industrial facilities. In the present research, Barkhausen Noise (BN) has been used to evaluate changes of mechanical properties due to heat treatment condition. The BN voltage (rms voltage) was measured with grain size. The rms voltage of BN increased with the heat treatment temperature ($870{\sim}1000^{\circ}C$) because the grain size increased with the temperature. The rms voltage of BN decreased with various heat treatment processes, such as quenched, tempered and PWHT. The BN can be used for the nondestructive evaluation of the forged reactor vessels. and moreover, it may be effectively used in the field application.

• Journal of Ceramic Processing Research
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• v.17 no.7
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• pp.763-767
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• 2016

The structural characterization of cubic boron nitride (c-BN) thin films was performed using a B4C target in a radio-frequency magnetron sputtering system. The deposition processing conditions, including the substrate bias voltage, substrate temperature, and base pressure were varied. Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy were used to analyze the crystal structures and chemical binding energy of the films. For the BN film deposited at room temperature, c-BN was formed in the substrate bias voltage range of -400 V to -600 V. Less c-BN fraction was observed as the deposition temperature increased, and more c-BN fraction was observed as the base pressure increased.