• Title/Summary/Keyword: BM S

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Genetic Diversity of Magra Sheep from India Using Microsatellite Analysis

  • Arora, R.;Bhatia, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.7
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    • pp.938-942
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    • 2006
  • Genetic diversity of Magra - a lustrous carpet wool breed of India, was investigated by means of 25 ovine microsatellite markers proposed by the Food and Agriculture Organization and the International Society for Animal Genetics (FAO-ISAG). All used microsatellites amplified well and exhibited polymorphisms. A wide range of genetic variability was observed as allele number from 3 (BM6506, OarCP20) to 10 (CSSM31), observed heterozygosity from 0.200 (BM6506) to 0.947 (OarHH35), expected heterozygosity from 0.368 (CSSM47) to 0.864 (BM1314) and Polymorphism Information Content (PIC) from 0.347 (CSSM47) to 0.849 (BM1314). This supported the utility of these microsatellite loci in the measurement of genetic diversity indices in Indian sheep too. Various average genetic variability measures viz., allele diversity (5.7), observed heterozygosity (0.597), expected heterozygosity (0.694) and mean PIC (0.648) values showed high genetic variability despite accumulated inbreeding as reflected by the high average inbreeding coefficient ($F_{IS}=0.159$) due to the unequal sex ratio of the breeding animals.

Bombyx mori β-tubulin Promoter for High-level Expression of Heterologous Genes

  • Park, Kwanho;Goo, Tae-Won
    • International Journal of Industrial Entomology and Biomaterials
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    • v.39 no.1
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    • pp.22-28
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    • 2019
  • We previously isolated 9 clones that show stronger signal compared to Bombyx mori cytoplasmic actin gene (BmA3) by using a dot blot hybridization. In this study, we focused on one clone among these clones which has high amino acid similarity with ${\beta}$-tubulin gene of B. mori. This clone was ubiquitously expressed in all tissues and developmental stage of B. mori. As result of promoter assay using dual luciferase assay system, we found the highest transcription activity region (-750/-1) in the 5'-flanking region of ${\beta}$-tubulin gene, which has about 47 fold more intensive promoter activity than BmA3 promoter. Moreover, the ${\beta}$-tubulin promoter was normally regulated in Bm5, Sf9, and S2 cells. Therefore, we suggest that ${\beta}$-tubulin promoter may be used more powerful and effectively for transgene expression in various insects containing B. mori as a universal promoter.

Mechanical properties and microstructure of innovative bio-mortar containing different aggregates

  • Abo-El-Eanein, S.A.;Abdel-Gawwad, H.A.;El-Mesallamy, Amani M.D.;El-Belbasi, Hussein I.;Ayoub, Hebah. G.
    • Geosystem Engineering
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    • v.21 no.5
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    • pp.291-296
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    • 2018
  • The aim of this work is to study the effect of aggregate type on the physico-mechanical properties and microstructure of bio-mortar (BM). Three different aggregates such as sand, dolomite and basalt were used. BM was prepared by mixing aggregates with bacterial cells (Sporosarcina Pasteurii) and one equimolar (1 M) of $urea/CaCl_2.2H_2O$. The results proved that the chemical composition and physical properties of aggregates play an important role in the microbial precipitation rate as well as size, morphology and crystallinity of the precipitated calcite, which strongly reflects on the properties of the prepared BM. The BM containing dolomite gave the highest compressive strength and lowest water absorption.

Medium to Long Range Wireless Video Transmission Scheme in 2.4GHz Band with Beamforming (빔 형성을 적용한 2.4GHz 대역 중장거리 영상 전송 무선 기술)

  • Paik, Junghoon;Kim, Namho;Jee, Minki
    • Journal of Broadcast Engineering
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    • v.23 no.5
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    • pp.693-700
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    • 2018
  • In this paper, we propose a wireless video transmission scheme, providing medium and long range communication in the 2.4GHz band with beamforming. With this scheme, it is shown that the transmission rate of 32Mbps and received signal power of -77dBm is achieved with 4 antennas of 5dBi and 16dBm transmit power at each antenna connection for the distance of 3.6km. The scheme also provides transmission distance of 20km for 10~12Mbps with the 4 omni-directional antennas of 5dBi.

A Design of High Power Amplifier Predistortor using Carrier Complex Power Series Analysis (Carrier Complex Power Series 해석을 통한 대전력 증폭기용 전치 왜곡기 설계)

  • 윤상영;정용채
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.12 no.5
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    • pp.686-693
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    • 2001
  • In this paper, a new carrier complex power series which represents nonlinear transfer function of high power amplifier is derived. Using this transfer function, the nonlinear transfer function of predistortive circuit for linearizing the distortion effect of a HPA(High Power Amplifier) is derived and fabricated. A measured gain and $P_{1dB}$ of the fabricated HPA in IMT-2000 basestation transmitting band are 34.06 dB and 35.4 dBm. The predistortive circuit using inverse carrier complex power series is fabricated and operated with HPA. The predistortive HPA improves C/I(Carrier to Intermodulation) ratio of HPA by 17.01 dB(@Pout=25.43 dBm/tone) with 2-tone at 2.1375 GHz and 2.1425 GHz.

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The Impact of Energy Crisis and Political Instability on Outsourcing: An Analysis of the Textile Industry of Pakistan

  • ARSLAN, Aniqa;QAYYUM, Arslan;AYUBI, Sharique;KHAN, Sohail Ahmed;ASAD ULLAH, Muhammad
    • The Journal of Asian Finance, Economics and Business
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    • v.9 no.3
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    • pp.235-243
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    • 2022
  • To help the industry, outsourcing was found to be the most efficient method. An extensive literature analysis was done to assess the macroeconomic factors associated with outsourcing to supplement the anxious parties' decision-making process with evidence-based comprehensive tools. As a theoretical framework for evaluating these issues, transaction cost economies and resource-based perspective theories are investigated. Outsourcing is proven to be a result of energy crises and political instability. The advantages of outsourcing assist major industries in the economy. To discover the key drivers behind outsourcing, we used the vector autoregressive (VAR model) and step-wise regression techniques for the period 1992 to 2016. This research adds to the literature in that it not only explains the energy issue but also discusses the dilemma of political instability in the country in the context of outsourcing. The findings indicate that labor cost and export tendency have a positive impact on outsourcing strategy, which confirms the study's third and fourth hypotheses. Customs tax, inflation, and the unemployment rate, on the other hand, have a negative impact on textile outsourcing in Pakistan, according to the study's fifth, sixth, and seventh hypotheses.

A $0.13-{\mu}m$ CMOS RF Front-End Transmitter for LTE-Advanced Systems (LTE-Advanced 표준을 지원하는 $0.13-{\mu}m$ CMOS RF Front-End 송신기 설계)

  • Kim, Jong-Myeong;Lee, Kyoung-Wook;Park, Min-Kyung;Choi, Yun-Ho;Jung, Jae-Ho;Kim, Chang-Wan
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2011.10a
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    • pp.402-403
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    • 2011
  • This paper has proposed a $0.13-{\mu}m$ CMOS RF Front-end transmitter for LTE-Advanced systems. The proposed RF Front-end supports a band 7 (from 2500 MHz to 2570 MHz) in E-UTRA of 3GPP. It can provide a maximum output power level of +10 dBm but it's a normal output power level is +0 dBm considering a low PAPR. The post-layout simulation results show that the quadrature up-conversion mixer and a driver amplifier consumes 14 mA and 28 mA from a 1.2 V supply voltage respectively, while providing a output power level of 0 dBm at the input power level of -13 dBm.

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Elizabethkingia miricola BM10, a New Symbiotic Bacterium Isolated from the Hindgut of the Termite Reticulitermes speratus KMT001

  • LEE, Dongmin;KIM, Young-Kyoon;KIM, Yeong-Suk;KIM, Tae-Jong
    • Journal of the Korean Wood Science and Technology
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    • v.47 no.6
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    • pp.692-699
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    • 2019
  • Elizabethkingia miricola BM10, a symbiotic bacterium, has been isolated from the hindgut of Reticulitermes speratus KMT001, a termite which occurs on Bukhan Mountain in Seoul, Korea. This strain demonstrated a symbiotic characteristic, in that it lacked endo-${\beta}$-1,4-glucanase activity, in a previous study. The major fatty acids of E. miricola BM10 were iso-$C_{15:0}$, iso-$C_{17:0}$ 3-OH, and summed feature 3 (iso-$C_{16:1}{\omega}7c/C_{16:1}{\omega}6c$). The content of iso-$C_{17:0}$ 3-OH was higher, while those of ECL 13.566, iso-$C_{17:11}{\omega}9c$, and summed feature 4 were lower than the other three type-strains of the Elizabethkingia genus. The 16S rRNA phylogenetic analysis confirmed that E. miricola BM10 is a new species. The whole genome of E. miricola BM10 was sequenced. The average nucleotide identity of strain BM10 as evaluated by pairwise comparison with E. anophelis R26, E. meningoseptica ATCC 13253, and E. miricola GTC 862 was shown to be 91.5%, 81.2%, and 94.29%, respectively. Based on our study results, E. miricola BM10 appears to represent a new strain of the genus Elizabethkingia.

Propagation of Bombyx mori Nucleopolyhedrovirus in Nonpermissive Insect Cell Lines

  • Woo, Soo-Dong;Roh, Jong-Yul;Choi, Jae-Young;Jin, Byung-Rae
    • Journal of Microbiology
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    • v.45 no.2
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    • pp.133-138
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    • 2007
  • This study addresses the susceptibility of Spodoptera frugiperda (Sf9 and Sf21), Trichoplusia ni (Hi5), and S. exigua (Se301) cells to the Bombyx mori nucleopolyhedrovirus (BmNPV). Although these cells have classically been considered nonpermissive to BmNPV, the cytopathic effect, an increase in viral yield, and viral DNA synthesis by BmNPV were observed in Sf9, Sf21, and Hi5 cells, but not in Se301 cells. Very late gene expression by BmNPV in these cell lines was also detected via ${\beta}-galactosidase$ expression under the control of the polyhedrin promoter. Sf9 cells were most susceptible to BmNPV in all respects, followed by Sf21 and Hi5 cells in decreasing order, while the Se301 cells evidenced no distinct viral replication. This particular difference in viral susceptibility in each of the cell lines can be utilized for our understanding of the mechanisms underlying the host specificity of NPVs.

Effect of Bitter Melon on Plasma Blood Glucose and Cholesterol Levels in Streptozotocin Induced Diabetic Rats (당뇨 유발 흰쥐에서 여주열매 첨가 시 혈당과 콜레스테롤 수준에 미치는 영향)

  • Kim, Myung-Wha
    • Journal of the East Asian Society of Dietary Life
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    • v.23 no.6
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    • pp.704-712
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    • 2013
  • This study was designed to examine the effects of bitter melon (BM) on the plasma blood glucose and cholesterol levels in diabetic rats. Diabetes mellitus was induced in male Sprague-Dawley rats through an injection of streptozotocin (STZ) dissolved in a citrate buffer into the tail vein at a dose of 45 mg/kg of body weight. Sprague-Dawley rats were then fed for four weeks, with the experimental groups receiving a modified diet containing 5% or 10% powder derived from BM. The experimental groups were divided into 4 groups, consisting of the normal control group, STZ-control group and diabetic fed with BM 5% & 10% treated groups. The rats' body weight, blood glucose and cholesterol values were measured along with the hematocrit (Hct) values and aminotransferase activities. Body weight losses were observed in the diabetic groups, whereas the control rats gained weight. There were significant differences in kidney weight between the control group and the diabetic groups. The Hct levels of the diabetic BM-treated group were significantly higher than the STZ-control group. Aspartate aminotransferase activity was lower in the non-diabetic group compared to the diabetic experimental groups. Further, the blood glucose was significantly decreased in the 5% & 10% BM of the diabetic group. There were no significant difference in cholesterol levels among the diabetic groups. These results indicate that the supplementation of bitter melon may have a favorable influence on reducing the blood glucose level in STZ-induced diabetic rats.