Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
권호 표지
DOI QR코드

DOI QR Code

Elizabethkingia miricola BM10, a New Symbiotic Bacterium Isolated from the Hindgut of the Termite Reticulitermes speratus KMT001

  • LEE, Dongmin (Department of Forest Products and Biotechnology, College of Science and Technology, Kookmin University) ;
  • KIM, Young-Kyoon (Department of Forest Products and Biotechnology, College of Science and Technology, Kookmin University) ;
  • KIM, Yeong-Suk (Department of Forest Products and Biotechnology, College of Science and Technology, Kookmin University) ;
  • KIM, Tae-Jong (Department of Forest Products and Biotechnology, College of Science and Technology, Kookmin University)
  • Received : 2019.03.01
  • Accepted : 2019.10.18
  • Published : 2019.11.25
Download PDF
⟨ Previous Next ⟩

Abstract

Elizabethkingia miricola BM10, a symbiotic bacterium, has been isolated from the hindgut of Reticulitermes speratus KMT001, a termite which occurs on Bukhan Mountain in Seoul, Korea. This strain demonstrated a symbiotic characteristic, in that it lacked endo-${\beta}$-1,4-glucanase activity, in a previous study. The major fatty acids of E. miricola BM10 were iso-$C_{15:0}$, iso-$C_{17:0}$ 3-OH, and summed feature 3 (iso-$C_{16:1}{\omega}7c/C_{16:1}{\omega}6c$). The content of iso-$C_{17:0}$ 3-OH was higher, while those of ECL 13.566, iso-$C_{17:11}{\omega}9c$, and summed feature 4 were lower than the other three type-strains of the Elizabethkingia genus. The 16S rRNA phylogenetic analysis confirmed that E. miricola BM10 is a new species. The whole genome of E. miricola BM10 was sequenced. The average nucleotide identity of strain BM10 as evaluated by pairwise comparison with E. anophelis R26, E. meningoseptica ATCC 13253, and E. miricola GTC 862 was shown to be 91.5%, 81.2%, and 94.29%, respectively. Based on our study results, E. miricola BM10 appears to represent a new strain of the genus Elizabethkingia.

Keywords

References

  1. Bernardet, J.-F., Hugo, C., Bruun, B. 2006. The genera Chryseobacterium and Elizabethkingia, pp. 638-676. In Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K.-H., Stackebrandt, E. (ed.) The Prokaryotes. Springer. New York, USA.
  2. Brune, A., Ohkuma, M. 2011. Role of the termite gut microbiota in symbiotic digestion, pp. 439-475. In Bignell, D.E., Roisin, Y., Lo, N. (ed.) Biology of termites: a modern synthesis. Springer. New York, USA.
  3. Ceyhan, M., Celik, M. 2011. Elizabethkingia meningosepticum (Chryseobacterium meningosepticum) infections in children. International Journal of Pediatrics 2011: Article ID 215237.
  4. Cho, M.-J., Kim, Y.-H., Shin, K., Kim, Y.-K., Kim, Y.-S., Kim, T.-J. 2010. Symbiotic adaptation of bacteria in the gut of Reticulitermes speratus: Low endo-${\beta}$-1,4-glucanase activity. Biochemical and Biophysical Research Communications 395(3): 432-435. https://doi.org/10.1016/j.bbrc.2010.04.048
  5. Cho, M., Shin, K., Kim, Y.-K., Kim, Y.-S., Kim, T.-J. 2010. Phylogenetic analysis of Reticulitermes speratus using the mitochondrial cytochrome C oxidase subunit I gene. Journal of the Korean Wood Science and Technology 38(2): 135-139. https://doi.org/10.5658/WOOD.2010.38.2.135
  6. Doijad, S., Ghosh, H., Glaeser, S., Kampfer, P., Chakraborty, T. 2016. Taxonomic reassessment of the genus Elizabethkingia using whole-genome sequencing: Elizabethkingia endophytica Kampfer et al. 2015 is a later subjective synonym of Elizabethkingia anophelis Kampfer et al. 2011. International Journal of Systematic and Evolutionary Microbiology 66(11): 4555-4559. https://doi.org/10.1099/ijsem.0.001390
  7. Kampfer, P., Matthews, H., Glaeser, S.P., Martin, K., Lodders, N., Faye, I. 2011. Elizabethkingia anophelis sp. nov., isolated from the midgut of the mosquito Anopheles gambiae. International Journal of Systematic and Evolutionary Microbiology 61(11): 2670-2675. https://doi.org/10.1099/ijs.0.026393-0
  8. Kampfer, P., Busse, H.-J., McInroy, J.A., Glaeser, S.P. 2015. Elizabethkingia endophytica sp. nov., isolated from Zea mays and emended description of Elizabethkingia anophelis Kampfer et al. 2011. International Journal of Systematic and Evolutionary Microbiology 65(7): 2187-2193. https://doi.org/10.1099/ijs.0.000236
  9. Kim, K.K., Kim, M.K., Lim, J.H., Park, H.Y., Lee, S.-T. 2005. Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov. International Journal of Systematic and Evolutionary Microbiology 55(3): 1287-1293. https://doi.org/10.1099/ijs.0.63541-0
  10. Kim, S.H., Chung, Y.J. 2017. Ingestion toxicity of fipronil on Reticulitermes speratus kyushuensis (Isoptera: Rhinotermitidae) and its applicability as a termite bait. Journal of the Korean Wood Science and Technology 45(2): 159-167. https://doi.org/10.5658/WOOD.2017.45.2.159
  11. King, E.O. 1959. Studies on a group of previously unclassified bacteria associated with meningitis in infants. American Journal of Clinical Pathology 31(3): 241-247. https://doi.org/10.1093/ajcp/31.3.241
  12. Kukutla, P., Lindberg, B.G., Pei, D., Rayl, M., Yu, W., Steritz, M., Faye, I., Xu, J. 2013. Draft genome sequences of Elizabethkingia anophelis strains $R26^T$ and Ag1 from the mid gut of the malaria mosquito Anopheles gambiae. Genome Announcements 1(6): e01030-01013.
  13. Lee, A.-H., Jang, J.-H., Hwang, W.-J., Kim, N.-H. 2017. Decay and termite resistance of yellow-hearted pine (Pinus densiflora for. erecta Uyeki). Journal of the Korean Wood Science and Technology 45(1): 12-19. https://doi.org/10.5658/WOOD.2017.45.1.12
  14. Lee, D., Kim, Y.-K., Kim, Y.-S., Kim, T.-J. 2015. Complete genome sequence of Elizabethkingia sp. BM10, a symbiotic bacterium of the wood-feeding termite Reticulitermes speratus KMT1. Genome Announcements 3(5): e01181-01115.
  15. Lee, D., Kim, Y.-S., Kim, Y.-K., Kim, T.-J. 2018. Symbiotic bacterial flora changes in response to low temperature in Reticulitermes speratus KMT001. Journal of the Korean Wood Science and Technology 46(6): 713-725. https://doi.org/10.5658/WOOD.2018.46.6.713
  16. Li, Y., Kawamura, Y., Fujiwara, N., Naka, T., Liu, H., Huang, X., Kobayashi, K., Ezaki, T. 2003. Chryseobacterium miricola sp. nov., a novel species isolated from condensation water of space station Mir. Systematic and Applied Microbiology 26(4): 523-528. https://doi.org/10.1078/072320203770865828
  17. Matyi, S.A., Hoyt, P.R., Hosoyama, A., Yamazoe, A., Fujita, N., Gustafson, J.E. 2013. Draft genome sequences of Elizabethkingia meningoseptica. Genome Announcements 1(4): e00444-00413.
  18. Miller, L.T. 1982. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. Journal of Clinical Microbiology 16(3): 584-586. https://doi.org/10.1128/JCM.16.3.584-586.1982
  19. Rodriguez-R, L.M., Konstantinidis, K.T. 2016. The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes. PeerJ Preprints 4: e1900v1.
  20. Wonhoon, L., Choi, D.-S., Ji, J.-Y., Kim, N., Han, J.M., Park, S.-H., Lee, S., Seo, M.S., Hwang, W.-J., Forschler, B., Takematsu, Y., Lee, Y.-H. 2015. A new record of Reticulitermes kanmonensis Takematsu, 1999 (Isoptera: Rhinotermitidae) from Korea. Journal of Asia-Pacific Entomology 18(3): 351-359. https://doi.org/10.1016/j.aspen.2015.04.006