International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Bombyx mori β-tubulin Promoter for High-level Expression of Heterologous Genes

  • Park, Kwanho (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Goo, Tae-Won (Department of Biochemistry, School of Medicine, Dongguk University)
  • Received : 2019.07.31
  • Accepted : 2019.09.09
  • Published : 2019.09.28
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Abstract

We previously isolated 9 clones that show stronger signal compared to Bombyx mori cytoplasmic actin gene (BmA3) by using a dot blot hybridization. In this study, we focused on one clone among these clones which has high amino acid similarity with ${\beta}$-tubulin gene of B. mori. This clone was ubiquitously expressed in all tissues and developmental stage of B. mori. As result of promoter assay using dual luciferase assay system, we found the highest transcription activity region (-750/-1) in the 5'-flanking region of ${\beta}$-tubulin gene, which has about 47 fold more intensive promoter activity than BmA3 promoter. Moreover, the ${\beta}$-tubulin promoter was normally regulated in Bm5, Sf9, and S2 cells. Therefore, we suggest that ${\beta}$-tubulin promoter may be used more powerful and effectively for transgene expression in various insects containing B. mori as a universal promoter.

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References

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