• Title/Summary/Keyword: BLB

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Low Temperature Growth of Silicon Oxide Thin Film by In-direct Contacting Process with Photocatalytic TiO2 Layer on Fused Silica (광촉매 TiO2 층의 비접촉식 공정을 통한 저온 실리콘 산화박막 성장)

  • Ko, Cheon Kwang;Lee, Won Gyu
    • Applied Chemistry for Engineering
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    • v.19 no.2
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    • pp.236-241
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    • 2008
  • The possibility of silicon oxidation through the aerial-diffusion of active oxygen species has been evaluated. The species originate from the surface of $TiO_2$ exposed by UV. Among process parameters such as UV intensity, substrate temperature and chamber pressure with oxygen, UV intensity was a major parameter to the influence on the oxide growth rate. When 1 kW high pressure Hg lamp was used as a UV source, the growth rate of silicon oxide was 8 times as faster as that of a 60 W BLB lamp. However, as the chamber pressure increased, the growth rate was declined due to the suppression of aerial diffusion of active oxygen species. According to the results, it could be confirmed that the aerial-diffusion of active oxygen species from UV-irradiated photocatalytic surface can be applied to a new method for preparing an ultra-thin silicon oxide at the range of relatively low temperature.

Rice blast susceptible mutants of Taebaegbyeo and genes differentially expressed in he wild type rice.

  • Lee, C. H.;C. U. Han;K. S. Jang;Park, Y. H.;H. K. Lim;Kim, J.C.;Park, G. J.;J.S. Cha;Park, J. E.
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.67.2-68
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    • 2003
  • A rice cultivar, Taebaegbyeo, is highly resistant to rice blast and moderately resistant to bacterial leaf blight (BLB) caused by Magnaporthe grisea and Xanthomonas oryzae pv. oryzae, respectively. To study the rice disease resistance mechanism, we generated rice deletion M3 mutants by gamma-ray irradiation. Blast and BLB responses of 16,000 M3 mutants were screened by inoculating mixtures of 4 races (KJ-201, H-1113a, KI-313, KI-409) of M. grisea and 3 Korean races of X. oryzae pv. oryzae. We selected so far 21 M3 mutants of Taebaegbyeo showing high susceptibility to the diseases. One of the mutants, KCT-6417, was susceptible to KI-1113a race of M. grisea, suggesting the deletion of a race-specific blast resistance gene in the mutant. To isolate rice genes involved in blast resistance and defense response, we take a PCR-based suppression subtractive hybridization approach using cDNAs of blast-inoculated wild type and the KCT-6417 as a tester and a driver, respectively. Genes specifically expressed in the wild type will be presented. The selected genes would give us a clue to understand mechanism for the race specific resistance and defense responses against M. grisea H-1113a in Taebaegbyeo.

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Decolorization of Rhodamine B using Rotating Disk Photocatalytic Reactor (회전원판 광촉매 반응기(Rotating Disk Photocatalytic Reactor)를 이용한 Rhodamine B의 색 제거)

  • Park, Young Seek
    • Journal of Korean Society on Water Environment
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    • v.21 no.1
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    • pp.46-51
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    • 2005
  • The photocatalytic oxidation of Rhodamine B (RhB) was studied using immobilized $TiO_2$ and rotating disk photocatalytic reactor. Immobilized $TiO_2$ onto the surface of the aluminum plate was employed as the photocatalyst and two 20 W germicidal lamps and two 20 W UV-BLB lamps were used as the light source and the reactor volume was 1.0 L. The effects of parameters such as the number of rotating disk, rpm of rotating disk, the number of coating, $H_2O_2$ and photo-fenton amounts, and the concentrations of anions and cations ($NO_3{^-}$, $SO_4{^{2-}}$, $Cl^-$, $Ca^{2+}$, $Zn^{2+}$, $Na^+$) were examined.

Characteristics of Residual Ozone Decomposition with Commercial Ozone Decomposition Catalyst (ODC) and Photo catalyst (상업용 오존촉매와 광촉매를 이용한 오존제거특성)

  • Byeon, Jeong-Hoon;Park, Jae-Hong;Hwang, Jung-Ho
    • Proceedings of the KSME Conference
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    • 2004.04a
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    • pp.1255-1260
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    • 2004
  • Decomposition of ozone at room temperature was investigated comparatively with commercial monolithic ozone decomposition catalyst (ODC, $MnO_2$) and monolithic photo catalyst ($TiO_2$). The effects of residence time, UV (ultraviolet) light dependence and ozone concentration on the conversion was presented. UV ray was irradiated using BLB (black light blue) lamp ($315{\sim}400$ nm), supplied with a constant intensity in the reactor. The concentration of ozone in the square-shape reactor can be controlled by combining the DBD (dielectric barrier discharge) reactor with an AC high voltage supply system. The catalytic performance, in presence of UV irradiation did not show significant changes for $MnO_2$ catalyst. $TiO_2$ catalyst was the different case, which showed higher decomposition activity in presence of UV irradiation. Deactivation of catalyst detected by real-time ozone monitor for 120 hours with a constant inlet ozone concentration.

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A Rapid and Simple Detection Assay for Rice Bacterial Leaf Blight by Recombinase Polymerase Amplification (벼 흰잎마름병의 신속하고 간편한 진단을 위한 Recombinase Polymerase Amplification 등온증폭법)

  • Kim, Shinhwa;Lee, Bong Choon;Kim, Hyun Ju;Choi, Soo Yeon;Seo, Su Jwa;Kim, Sang-Min
    • Research in Plant Disease
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    • v.26 no.4
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    • pp.195-201
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    • 2020
  • Rice bacterial leaf blight (BLB) by Xanthomonas oryzae pv. oryzae (Xoo) is considered to be one of the major rice diseases steadily occurring around the rice-producing countries. In this study, we developed a recombinase polymerase amplification (RPA) assay for the rapid, convenient and specific diagnosis of Xoo by targeting Xoo-specific transposase A gene. As the target gene can be amplified in 10 min without DNA extraction process and special equipment for temperature control, RPA for BLB can be useful and practical component for on-site diagnosis.

DNA Microarray and Gene Ontology Enrichment Analysis Reveals That a Mutation in opsX Affects Virulence and Chemotaxis in Xanthomonas oryzae pv. oryzae

  • Kim, Hong-Il;Park, Young-Jin
    • The Plant Pathology Journal
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    • v.32 no.3
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    • pp.190-200
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    • 2016
  • Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight (BLB) in rice (Oryza sativa L.). In this study, we investigated the effect of a mutation in opsX (XOO1056), which encodes a saccharide biosynthesis regulatory protein, on the virulence and bacterial chemotaxis of Xoo. We performed DNA micro-array analysis, which showed that 63 of 2,678 genes, including genes related to bacterial motility (flagellar and chemotaxis proteins) were significantly downregulated ($<\;-2\;log_2$ fold changes) by the mutation in opsX. Indeed, motility assays showed that the mutant strain was nonmotile on semisolid agar swarm plates. In addition, a mutant strain (opsX::Tn5) showed decreased virulence against the susceptible rice cultivar, IR24. Quantitative real-time RT-PCR reaction was performed to confirm the expression levels of these genes, including those related to flagella and chemotaxis, in the opsX mutant. Our findings revealed that mutation of opsX affects both virulence and bacterial motility. These results will help to improve our understanding of Xoo and provide insight into Xoo-rice interactions.

Oomycetes RXLR Effectors Function as Both Activator and Suppressor of Plant Immunity

  • Oh, Sang-Keun;Kamoun, Sophien;Choi, Doil
    • The Plant Pathology Journal
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    • v.26 no.3
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    • pp.209-215
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    • 2010
  • Plant pathogenic oomycetes, such as Phytophthora spp., are the causal agent of the most devastating plant diseases. During infection, these pathogens accomplish parasitic colonization of plants by modulating host defenses through an array of disease effector proteins. These effectors are classified in two classes based on their target sites in the host plant. Apoplastic effectors are secreted into the plant extracellular space, and cytoplasmic effectors are translocated inside the plant cell, through the haustoria that enter inside living host cell. Recent characterization of some oomycete Avr genes showed that they encode effector protein with general modular structure including N-terminal conserved RXLR-DEER motif. More detailed evidences suggest that these AVR effectors are secreted by the pathogenic oomycetes and then translocated into the host plant cell during infection. Recent findings indicated that one of the P. infestans effector, Avrblb2, specifically induces hypersensitive response (HR) in the presence of Solanum bulbocastanum late blight resistance genes Rpi-blb2. On the other hand, another secreted RXLR protein PexRD8 originated from P. infestans suppressed the HCD triggered by the elicitin INF1. In this review, we described recent progress in characterized RXLR effectors in Phytophthora spp. and their dual functions as modulators of host plant immunity.

Resistance of Varieties Bred by Crossing with Asominori to Bacterial Leaf Blight (벼 아소미노리 교배조합 품종의 벼흰잎마름병 저항성)

  • 심재성
    • Korean Journal of Plant Resources
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    • v.10 no.2
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    • pp.128-134
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    • 1997
  • This study was conducted to test on the resistance of varieties bred by crossing with Asominori to bacterial leaf blight. Nakdongbyeo and Dongjinbyeo which were susceptible to HB 9011, 8 resistant varieties including Ilmibyeo derived from Asominori and Asominori, Hwangok, 13 varieties including Chukoku 45 which were resistance to HB 9011, HB 9022 and HB 9033 were used to screen their res ponce depending on the various screening methods such as the true resistance, the secondary infection resistance and the disease common field test methods, and the results are as follows: Among 13 varieties tested, 11 varieties including llmibyeo showed tme resistance to HB 9011. Less than 1.0cm of disease lesion were developed on these varieties. Disease lesion was not developed on most of the Asominori lines including Daechongbyeo against IIB9011 and 1lmibyeo was also resistance to HB 9011, on this variety disease lesion area rate was 1.2%, and 7 varieties including Hwajinbyeo showed field resistance to HB 9022. Disease lesion area rate were 19.6% on Nakdongbyeo. 15.6% on Dongjinbyeo, from 3.0% to 2.4% on Asominori lines, and 0.5% on the Asominori when screened at disease common area. Disease was not developed on Keumnambyeo. Significant correlation coefficiences were found between the results from the test methods of the true resistance, the field resistance ancl the field resistance at disease common field tests on Asominori lines, but in some cases, even the varieties on which disease lesions developed, showed field resistance to HB 9022 and HB 9033.

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Susceptibility of Tongil type Rice Cultivar Milyang 30 previously Resistant to Xanthomonas campestris pv. oryzae ('밀양 30호'의 흰빛잎마름병 (백엽고병)이병화)

  • Choi Yong-Chul;Yun Myung-Soo;Sohn Jae-Kyun
    • Korean journal of applied entomology
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    • v.23 no.1 s.58
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    • pp.1-6
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    • 1984
  • This study was conducted to investigate the incidence of bacterial leaf blight (BLB) in Milyang 30 which was previously considered to be resistant to Xanthomonas campestris pv. oryzae Seeds of Milyang 30 were collected from Suweon, Milyang and Haenam. When rice plants from different source were inoculated at the maximum tillering stage and flag leaf stage, reactions of Milyang 30 were consistant. The susceptibility of Milyang 30 was found to be due to infection with tome isolates in the pathotype II of X. campestris pv. oryzae.

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Performance of RC moment frames with fixed and hinged supports under near-fault ground motions

  • Mohammadi, Mohammad Hossain;Massumi, Ali;Meshkat-Dini, Afshin
    • Earthquakes and Structures
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    • v.13 no.1
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    • pp.89-101
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    • 2017
  • The focus of this paper is the study on the seismic performance of RC buildings with two different connections at the base level under near-fault earthquakes. It is well-known that the impulsive nature of the near-fault ground motions causes severe damages to framed buildings especially at base connections. In the scope of this study, two types of 3-dimensional RC Moment Frames with Fixed Support (MFFS) and Hinged Support (MFHS) containing 5 and 10 stories are assessed under an ensemble of 11 strong ground motions by implementing nonlinear response history analysis. The most vulnerable locations of MFFS, are the connections of corner columns to foundation especially under strong earthquakes. On the other hand, using beams at the base level as well as hinged base connections in MFHS buildings, prevents damages of corner columns and achieves more ductile behavior. Results denote that the MFHS including Base Level Beams (BLB) significantly shows better behavior compared with MFFS, particularly under pulse-type records. Additionally, the first story beams and also interior components undergo more actions. Role of the BLBs are similar to fuses decreasing the flexural moments of the corner columns. The BLBs can be constructed as replaceable members which provide the reparability of structures.