• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.482-482
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• 2005

• Journal of Animal Science and Technology
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• 제53권5호
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• pp.409-418
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• 2011

복합생균제의 급여가 육계의 생산성, 도체특성, 분중 $NH_3$ 가스 생성량 및 장내 병원성 미생물 억제효과에 미치는 영향을 조사하기 위하여 토종 닭 육계 병아리 120수를 이용하여 9주간 시험을 실시하였다. 실험은 5처리 3반복의 완전임의 배치법으로 설계 하였으며, 처리 당 24수씩 배치하였다. 처리내용은 C (대조구로서 기초사료만 급여), T1 (기초사료에 항생제 첨가), T2 (기초사료에 생균제제 bio-Max$^{(R)}$ 첨가), T3 (기초사료에 생균제제 bio-${\alpha}$-Gold$^{(R)}$ 첨가), T4 (기초사료에 bio-MAX$^{(R)}$와 면역증강제인 ${\beta}$-glucan, 유기산의 추가 첨가)로 구분하였으며, 병아리들은 2주간의 적응기간을 거친 후 2~6주령은 육계전기사료, 6~9주령은 육계후기사료를 급여 하였다. 총 증체량은 항생제가 첨가된 T1에서 가장 높았으며(p<0.05), 대조구와 생균제 급여구 간에는 차이가 없었다. 사료 섭취량은 2~9주령에서는 T1과 T2에서 유의적으로 증가 하였다(p<0.05). 결과적으로 사료요구율은 대조구와 항생제 첨가구 그리고 T4에서 다른 처리구보다 낮게 나타났다(p<0.05). 처리구 별 생체중과 도체율은 T3와 T4에서 다른 처리구보다 높게 나타났으며, 가식 내장 무게는 간과 심장에서 T4가 유의하게 높게 나타났다(p<0.05). 계분 중의 $NH_3$ 가스 발생량은 6일에 최고치에 달했으며 9일 이후 감소하였다. 복합 생균제 제품급여 시 암모니아 가스 발생량에 대한 영향은 항생제 첨가 급여구에 비하여 낮게 나타났다. 생균제 첨가 급여 시 맹장 내 salmonella 수는 T4에서 현저히 감소하였고, 회장 내에서는 T2와 T4에서 감소하였다. 맹장 내 E. coli 수는 T4에서 현저히 감소하였고, 회장 내에서는 T1, T2와 T4에서 유의적으로 감소하였다(p<0.05). 복합 생균제 제품은 장내 병원성 미생물 수를 감소시켰으며, 특히 ${\alpha}$-Max$^{(R)}$ 제품에 면역증강제가 첨가된 복합 생균제의 효과가 다른 처리구보다 높았다. 따라서 복합생균제에 면역증강제의 혼합급여형태는 장내 병원성 미생물 억제측면에서 항생제를 대체할 수 있는 가능성이 높을 것으로 사료된다.

• 농업생명과학연구
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• 제45권1호
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• pp.67-77
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• 2011

본 연구는 돼지의 육성기 또는 비육기에 Bio 이온수 급여에 따른 성장, 혈액분석 및 육질 특성 평가를 위하여 실시하였다. 시험구는 Bio 이온수 무 급여구 (대조구), 육성기 급여구 및 비육기 급여구로 3시험구를 두었으며, 각 시험구 당 33두의 3원교잡종 $(Landrace{\times}Yorkshire{\times}Duroc)$ 돼지를 배치하였으며, 총 99두를 이용하여 사양시험을 수행하였다. Bio 이온수 급여는 육성돈과 비육돈의 성장과 사료효율에 영향을 미치지 않았지만 (P>0.05), 비육기 급여구에서 일당증체량과 A등급 출현율이 높게 나타났다. 대조구에 비해 Bio 이온수를 급여한 처리구에서 혈액성상 분석 결과 적혈구와 백혈구의 수치가 증가하였다 (p<0.05). 일반성분, 육색, pH, 육즙감량, 가열감량 및 전단력에서 유의적인 차이는 나타나지 않았다. 지방산 분석 결과 육성기 급여구에서 포화지방산/불포화지방산 비율이 낮게 나타났고, 불포화지방산의 함량 비율이 가장 높게 나타났다. 또한 가열육 관능검사에서 향과 전체적인 기호도에서 높은 점수를 획득하였다.

• Journal of Ecology and Environment
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• 제41권3호
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• pp.99-106
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• 2017

Background: Plants over-expressing Arabidopsis ABF3 (abscisic acid-responsive element-binding factor 3) have enhanced tolerance to various environmental stresses, especially drought. Using terminal restriction fragment length polymorphism (T-RFLP) analysis, we compared the rhizosphere-associated structures of microbial communities for transgenic potato containing this gene and conventional "Jopoong" plants. Results: During a 2-year field experiment, fungal richness, evenness, and diversity varied by year, increasing in 2010 when a moderate water deficit occurred. By contrast, the bacterial richness decreased in 2010 while evenness and diversity were similar in both years. No significant difference was observed in any indices for either sampling time or plant line. Although the composition of the microbial communities (defined as T-RF profiles) changed according to year and sampling time, differences were not significant between the transgenic and control plants. Conclusions: The results in this study suggest that the insertion of ABF3 into potato has no detectable (by current T-RFLP technique) effects on rhizosphere communities, and that any possible influences, if any, can be masked by seasonal or yearly variations.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2004년도 The 3rd Annual Conference for The Korean Society for Bioinformatics Association of Asian Societies for Bioinformatics 2004 Symposium
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• pp.77-85
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• 2004

A significant portion (about 8% in human genome) of mammalian mRNA sequences contains AU(Adenine and Uracil) rich elements or AREs at their 3' untranslated regions (UTR). These mRNA sequences are usually stable. ARE motifs are assorted into three classes. The importance of AREs in biology is that they make certain mRNA unstable. We analyzed the occurrences of AREs and Alu, and propose a possible mechanism on how human mRNA could acquire and keep A REs at its 3' UTR originated from Alu repeats. Interspersed in the human genome, Alu repeats occupy 5% of the 3' UTR of mRNA sequences. Alu has poly-adenine (poly-A) regions at the end that lead to poly -thymine (poly-T) regions at the end of its complementary Alu. It has been discovered that AREs are present at the poly -T regions. In the all ARE's classes, 27-40% of ARE repeats were found in the poly -T region of Alu with mismatch allowed within 10% of ARE's length from the 3' UTRs of the NCBI's reference m RNA sequence database. We report that Alu, which has been reported as a junk DNA element, is a source of AREs. We found that one third of AREs were derived from the poly -T regions of the complementary Alu.

• 한국농공학회논문집
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• 제57권3호
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• pp.121-127
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• 2015

The objective of this study is to suggest the water quality criteria of total nitrogen in order to efficiently manage the nutrient pollution in the stream. For this, correlations between water quality parameters were examined using the water quality data collected from the water quality monitoring network in the four rivers between 2003 and 2012. T-N showed positive correlations with T-P (0.636), COD (0.577), BOD (0.574), TOC (0.440), and SS (0.367). The statistical analysis including percentile analysis for the T-N and T-P concentrations was utilized to develop the water quality criteria of T-N. The feasibility of the suggested water quality criteria was evaluated by calculating the achievement rate to water quality target at the representative points in mid-watershed, then the draft water quality standard of T-N was suggested. The suggested water quality standard of T-N in the stream may be used to efficiently control the nutrient pollution in the public water body.

• Journal of Species Research
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• 제7권2호
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• pp.151-160
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• 2018

Seven bacterial strains, 15J4M-1, 15J13-8, 16MFM10, 15J1-8, SR1-5-4, 15J13-6, and 15J8-11 assigned to the phylum Actinobacteria, Bacteroidetes, and Firmicutes were isolated from soil samples collected from Jeju, Korea. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strains 15J4M-1, 15J13-8, 16MFM10, 15J1-8, SR1-5-4, 15J13-6, and 15J8-11 were most closely related to Bacillus selenatarsenatis $SF-1^T$ (with 99.4% similarity), Brevibacterium luteolum $CF87^T$ (99.5%), Carnobacterium iners CCUG $62000^T$ (99.6%), Exiguobacterium profundum $10C^T$ (99.3%), Larkinella insperata LMG $22510^T$ (99.3%), Pseudokineococcus lusitanus CECT $7306^T$ (99.4%), and Spirosoma endophyticum $EX36^T$ (99.3%), respectively. This is the first report of these seven species in Korea.

• 한국발생생물학회지:발생과생식
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• 제15권1호
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• pp.53-59
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• 2011

Sex steroid hormones are key molecules to prepare the decidual response and their levels are important in this process. Imbalances of the levels of steroid hormones are cause of implantation failure and other diseases including physical weakness. Androgen replacement therapy or selective androgen receptor modulator are used to overcome various diseases but long-term use may cause of side effects. In previous report, it is suggested that the steroid hormonal complexes derived from pig enhance the proliferation of satellite cell. Therefore, to evaluate the possible usage of steroid hormonal complex derived from pig testis (tS-C), the effects of tS-C on uterine response were studied using the model of artificial decidua. tS-C did not disturb the rhythmical estrus cycle. Artificial-induced decidual response was normally induced in tS-C administered mice. The histological characters of the decidua of tS-C administered mice were not different from the vehicle. The expression patterns of molecular markers of decidua were not different between vehicle and tS-C group. Collectively these results suggested that tS-C does not disturb the uterine responsibility to the embryo. In addition, our results suggested that tS-C can be applied to overcome the various problems such as loss of muscle mass and anemia.

• 한국물환경학회지
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• 제21권5호
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• pp.458-463
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• 2005

Considering the characteristics of a filtration bio-reactor equipped with a mesh filter module which can effectively maintain high concentration of biomass and enhanced solid-liquid separation performance, the hybrid process of filtration bio-reactor combined with coagulation was investigated to get improved filtration characteristics as well as water quality in this work. Two bio-reactors (Run-1 & Run-2) were operated under the following conditions: working volume of 25 L, continuous loading of a synthetic wastewater (BOD: 200 mg/L, T-N: 50 mg/L, T-P: 5 mg/L), where an appropriate amount of alum ($Al_2(SO_4)_3{\cdot}18H_2O$) was added once a day into the reactor (Run-2). In the system without using a alum (Run-1), the clogging of mesh filter module was observed two times through 85 days of whole operation. Meanwhile, the filter module did not clog even at higher MLSS concentration (6,000~12,000 mg/L) and the stable filtration (0.7 mid) was continued in the case of using a alum. Due to the stable formation of cake layers, BOD and SS were shown below 6 and 3 mg/L, respectively. T-P and pH of the effluent were changed because of the intermittent addition of the alum. In the case of Al/P=2.5, the average T-P removal efficiency per day was 85.2% and the average T-P concentration of the effluent was 0.3 mg/L. However, the removal efficiency of phosphate was influenced by pH in the reactor.

• 수산해양기술연구
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• 제57권2호
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• pp.117-126
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• 2021

Diet composition and trophic level of Trachurus japonicus were studied using 417 specimens collected by trawls, set nets and purse seine fisheries from March 2019 to February 2020 in the South Sea of Korea. The size of T. Japonicus ranged from 7.0 to 49.8 cm in total length. T. japonicus were a carnivore that fed mainly on euphausiids and pisces. In eddition, T. japonicus fed on small quantities of cephalopods, crabs etc. T. japonicus showed ontogenetic changes in feeding habits. The proportion of euphausiids decreased inversely proportional to body size whereas the consumption of pisces gradually increased. As body size of T. japonicus increased the mean number of prey per stomach and the mean weight of prey per stomach tended to increase, but the mean number of prey per stomach was not significantly different. As a result of the feeding strategy analysis, T. japonicus were specialized feeders with pisces and euphausiids as their dominant prey. The trophic level ranged between 3.57 ± 0.54 and 3.91 ± 0.65, and increased asymptotically with size of specimens. The average trophic level of the T. japonicus was 3.79 ± 0.61.