• BMB Reports
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• 제39권6호
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• pp.782-787
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• 2006

A new protein was cloned and identified as the sixth subunit of Choristoneura fumiferana origin recognition complex (CfORC6). The newly identified 43 kDa protein CfORC6 is much bigger than DmORC6 (25.7 kDa) and HsORC6 (28.1 kDa), though it's 23.85% identical to DmORC6 and 23.81% identical to HsORC6. Although the molecular weight of CfORC6 is close to ScORc6 (50 kDa), CfORC6 is only 14.03% identical to ScORC6. By alignment, it was found that the N-terminal of CfORC6 has about 30% identities with other ORC6s, but about 100aa of C-terminal of CfORC6 has no identity with other ORC6s. Like ScORC6, CfORC6 has many potential phosphorylation sites, (S/T)PXK. Like DmORC6, CfORC6 has leucine-rich region in the relevant site. Northern Blot showed that CfORC6 mRNA is about 2,000nt. Southern Blot confirmed that there is one copy of CfORC6 gene in spruce budworm genome. Western blot showed that infection of Cf124T cells with CfMNPV didn't affect the expression levels of CfORC6, at least up to 26 hr post infection.

• 근관절건강학회지
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• 제27권3호
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• pp.247-254
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• 2020

Purpose: This was a descriptive observational study examining the reasons for, frequency of, and factors affecting work interruption among intensive care unit (ICU) nurses. Methods: Convenience sampling was used to recruit 46 regular nurses who had been working in an ICU at D tertiary hospital in B metropolitan city for at least six months. To measure the degree of work interruption, this study revised a tool used by Brixey et al. (2007) for analyzing the type of work interruption. Results: A total of 1,787 work interruptions occurred during 368 hours of observation, i.e., at an average of 4.85 times per hour. Communication-related factors caused work interruption most frequently, followed by environmental, work-related, and personal factors. As for the work-related characteristics of nurses, participants experienced work interruption more frequently while working on weekdays, when the medical staff were stationed, than on weekends. Conclusion: ICU nurses experienced work interruption frequently. With the health care system expected to become more complicated in the future, efforts should be made to reduce unnecessary work interruptions to improve the operation efficiency of ICUs.

• Asian-Australasian Journal of Animal Sciences
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• 제18권6호
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• pp.787-794
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• 2005

The aim of this study was 1) to confirm the practical efficiency of a routine milk P4 monitoring system for postpartum reproductive management of a dairy herd, and 2) to evaluate the relationship between the blood metabolic profiles, milk quality and body weight of individual cows in the farm records, which may reflect the postpartum nutritional condition, and the time of postpartum resumption of ovarian activity of dairy cows. A total of 116 Holstein cows was used in the present study. First, during the period of Experiment 1, postpartum reproductive management based on weekly measured milk P4 concentration from individual cows was conducted. Compared with the reproductive records of the past two years without P4 monitoring, although the day from calving to first AI did not change, both the number of AI until pregnant (with P4; 1.9 times vs. without P4; 2.9 times) and the days open (with P4; 95.1 days vs. without P4; 135.8 days and 133.8 days) were significantly decreased. In Experiment 2, the measurement of blood constituents such as albumin, blood urea nitrogen, packed cell volume, ammonia, glucose, total cholesterol, non-esterified, AST and $\gamma$-GTP was performed on the blood samples taken once approximately 14 days postpartum, to monitor both health and nutritional conditions. The milk constituent parameters, such as milk protein (MP), milk fat (MF), SNF and lactose, collected from the monthly progeny test of individual cows, were used to monitor the postpartum nutritional status. Furthermore, the data obtained from the routine measurements of body weight were used to calculate the rate of peripartum body weight loss. The resumption day of the postpartum estrous cycle was assumed from the milk P4 profiles of individual cows. There was no clear relationship between each parameter from blood examination and those from resumption time. However, the cows had low values of MP, and SNF, which significantly affected the resumption of the postpartum estrous cycle. Similarly, a higher rate of body weight loss indicated a significant delay (more than 1 month) in the resumption of the postpartum estrous cycle, compared with the groups that had a medium or lower rate of body weight loss. The results of the present study demonstrated that the implementation of routine milk P4 monitoring-based postpartum reproductive management, together with milk quality parameters and routine BW data available in field conditions may be utilized as a practical approach for increasing the postpartum reproductive efficiency of a high yielding dairy herd.

• 과학교육연구지
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• 제41권2호
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• pp.179-194
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• 2017

이 연구의 목적은 CARS(Changes in Attitude of Relevance to Science)문항에 대한 신뢰도 및 타당도를 확인하여 우리나라 학생들에게의 적용여부를 판단하고, 성별 및 학교급별 차이를 비교한 후 라쉬 모델을 활용하여 두 개의 동형의 과학적 관련성 인식 검사도구를 구성하는 것이다. 이 연구를 위하여 59문항의 CARS 과학적 관련성 인식 검사문항을 번역하여 787명의 중 고등 (중학생 300명, 고등학생 431명의 답변이 분석됨) 학생들에게 투입하였다. CARS 문항의 우리나라 학생들에게의 적합도를 판단하고 많은 문항수의 제한점을 극복하기 위하여 라쉬 모델의 문항연결 방법을 활용하였다. 연구 결과를 분석하여 25문항으로 구성된 CARS-A와 CARS-B형의 두 과학적 관련성 인식 검사도구를 구성하였다. 두 동형검사도구의 라쉬 점수 상관관계는 0.78이며, 라쉬 모델 분석수치는 거의 일치하였다. 이 연구를 통해 생성된 두 형의 과학 관련성 인식 검사도구는 학생들의 과학의 일상관련 태도를 확인하거나, 특정 수업과정 후 또는 학년별 변화를 확인하는데 유용하게 활용될 수 있을 것이다. 이 연구를 통해 과학교육에서 학생들이 과학의 연관성 인식이 가지는 의미를 확인하고 도구개발 및 활용에 대하여 논의하고자 한다.

• 농촌의학ㆍ지역보건
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• 제45권2호
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• pp.89-99
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• 2020

이 연구는 연령대를 성인, 전기 노인, 후기 노인으로 구분 후 신체활동과 인지된 운동장애요인과의 관련성을 보고자 하였다. 연구 대상자는 두 군데 면 지역에 거주하고 있는 주민들 1500명을 대상으로 실시하였으며 걷기실천율의 정의는 주 5회 이상, 한번 할 때 10분 이상, 하루에 30분 이상 하는 것으로 하였고, 인지된 장애요인은 시간부족, 사회적 지지 부족, 기력 부족, 의지력 부족, 부상에 대한 공포 등 5개의 영역으로 조사하였다. 연령대를 세 개 군(~64세 이하, 65-74세, 75세 이상)으로 구분했을 때 의지력 부족이 모든 연령대에서 유의하였고, 64세 이하의 경우는 시간부족이 관련이 있었으며, 65세 이상은 부상에 대한 공포가 관련 있었다. 결론적으로 의지력 부족과 시간부족에 대한 해결을 위해서 마을 내에서 일정한 시간대에 함께 걷기운동을 실시하여 이를 해결하도록 하고, 노인들에게는 낙상예방을 위하여 걷기운동과 같은 유산소운동을 권장할 때 근력운동을 병행해 실시하여 부상에 대한 공포를 줄여야 할 것이다.

• 한국환경복원기술학회지
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• 제13권3호
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• pp.94-102
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• 2010

Carbon Neutral is one of the magnifying pan-governmental tasks which aim for stabilizing pan-global ecosystem. The purpose of this study is to estimates carbon neutral degree in cities by using capacity of carbon storage, which is carbon sinks, on forest and to develop an indicator that could be utilized in establishing policy related to climate change respond. When the carbon emitted from city has been absorbed or stored, the value of the indicator aiming to achieve carbon neutral could be drawn. The result and implication are as follows. First, while the annual amount of carbon emission in Gyeonggi S city was 18,787 thousand (tons), which was the highest, that of Gyeongbuk U city indicated the lowest with approximately 112 thousand (tons). Second, Gyeongbuk U city represented the largest capacity of annual carbon storage with about 16,748 thousand (tons), whereas the smallest figure was shown in Gyeonggi B city with 151 thousand (tons). Third, as result of the estimation of carbon neutral degree, the value of the indicator in Gyeonggi B city was 3% referring the lowest point, while that of other cities demonstrated over 100%. Forth, it has the possibility to suggest the fixed quantity when the plan for achieving carbon neutral of city is being processed. In the near future, after the completion of the construction projects of greenhouse gas inventory in all local governments, by utilizing the relative dates, the value of the indicator can be calculated and a more general conclusion could be drawn. Moreover, as expanding case studies to all domestic cities, generalness is in need.

• Journal of Microbiology and Biotechnology
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• 제30권3호
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• pp.417-426
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• 2020

Bacillus amyloliquefaciens is an important plant disease-preventing and growth-promoting microorganism. B. amyloliquefaciens WS-8 can stimulate plant growth and has strong antifungal properties. In this study, we sequenced the complete genome of B. amyloliquefaciens WS-8 by Pacific Biosciences RSII (PacBio) Single Molecule Real-Time (SMRT) sequencing. The genome consists of one chromosome (3,929,787 bp) and no additional plasmids. The main bacteriostatic substances were determined by genome, transcriptome, and mass spectrometry data. We thereby laid a theoretical foundation for the utilization of the strain. By genomic analysis, we identified 19 putative biosynthetic gene clusters for secondary metabolites, most of which are potentially involved in the biosynthesis of numerous bioactive metabolites, including difficidin, fengycin, and surfactin. Furthermore, a potential class II lanthipeptide biosynthetic gene cluster and genes that are involved in auxin biosynthesis were found. Through the analysis of transcriptome data, we found that the key bacteriostatic genes, as predicted in the genome, exhibited different levels of mRNA expression. Through metabolite isolation, purification, and exposure experiments, we found that a variety of metabolites of WS-8 exert an inhibitory effect on the necrotrophic fungus Botrytis cinerea, which causes gray mold; by mass spectrometry, we found that the main substances are mainly iturins and fengycins. Therefore, this strain has the potential to be utilized as an antifungal agent in agriculture.

• 한국전자파학회논문지
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• 제28권10호
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• pp.779-787
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• 2017

본 논문에서는 전원 스위칭 방법을 사용한 소형 무인기용 RF 송수신기에 대해 다루었다. 소형 무인기에 적용하기 위해서는 크기 및 무게 등의 물리적 고려 사항과 저전력 특성이 매우 중요한데, 본 논문에서는 전력 소모를 줄이기 위한 방법으로 전원 스위칭 방법을 제안하였다. 제안된 방법을 사용하여 소형 무인기에 적용이 가능한 RF 송수신기를 제작하여 성능을 확인하였다. 측정 결과, 출력은 +25 dBm, 잡음 지수는 4.56 dB, 수신 감도는 -100 dBm의 성능이 측정되었다. 소모 전력 측정 결과, 전원 스위칭 방법을 적용하면 약 25 % 정도의 전력 소모를 줄일 수 있었다. 제작된 RF 송수신기의 크기는 $100{\times}60{\times}5.7mm^3$이며, 무게는 38 g이다.

• 대한수학회지
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• 제36권4호
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• pp.787-811
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• 1999

Let M be the maximal ideal space of the Banach algebra $H^{\infty}$ of bounded analytic functions on the open unit disc $\triangle$. For a positive singular measure ${\mu}\;on\;{\partial\triangle},\;let\;{L_{+}}^1(\mu)$ be the set of measures v with $0\;{\leq}\;{\nu}\;{\ll}\;{\mu}\;and\;{{\psi}_{\nu}}$ the associated singular inner functions. Let $R(\mu)\;and\;R_0(\mu)$ be the union sets of $\{$\mid$\psiv$\mid$\;<\;1\}\;and\;\{$\mid${\psi}_{\nu}$\mid$\;<\;0\}\;in\;M\;{\setminus}\;{\triangle},\;{\nu}\;\in\;{L_{+}}^1(\mu)$, respectively. It is proved that if $S(\mu)\;=\;{\partial\triangle}$, where $S(\mu)$ is the closed support set of $\mu$, then $R(\mu)\;=\;R0(\mu)\;=\;M{\setminus}({\triangle}\;{\cup}\;M(L^{\infty}(\partial\triangle)))$ is generated by $H^{\infty}\;and\;\overline{\psi_{\nu}},\;{\nu}\;{\in}\;{L_1}^{+}(\mu)$. It is proved that %d{\theta}(S(\mu))\;=\;0$ if and only if there exists as Blaschke product b with zeros $\{Zn\}_n$ such that $R(\mu)\;{\subset}\;{$\mid$b$\mid$\;<\;1}\;and\;S(\mu)$ coincides with the set of cluster points of $\{Zn\}_n$. While, we proved that $\mu$ is a sum of finitely many point measure such that $R(\mu)\;{\subset}\;\{$\mid${\psi}_{\lambda}$\mid$\;<\;1}\;and\;S(\lambda)\;=\;S(\mu)$. Also it is studied conditions on \mu for which $R(\mu)\;=\;R0(\mu)$.

• 한국전기전자재료학회논문지
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• 제15권9호
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• pp.783-787
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• 2002

In recent year, B $i_{4-}$x L $a_{x}$ $Ti_3$ $O_{12(BLT)}$ is one of promising substitute materials for the ferroelectric random access memory(FRAM) applications. But the systematic composition is still insufficient, so this experiment was carried out in ceramic ambient sintering process which has the very excellent ferroelectric property. Samples were prepared by a bulk and the purpose which was estimated with a suitability of thin films applications. The density of B $i_{3.75}$ L $a_{0.25}$ $Ti_3$ $O_{12}$ was high and the XRD pattern showed that the intensity of main peak (117) was increased at the argon ambient sintering. Controlling the quantity of oxygen, crystallization showed a thin, long plate like type, and we obtained the excellent dielectric and polarization properties at the argon atmosphere sintering. Also this sintering process was effective at the bulk sample. Argon ambient sintered sample produced higher permittivity of 154, the remanent polarization(2Pr) of 6.8 uC/$\textrm{cm}^2$ compared with that sintered in air and oxygen ambient. And this sintering process showed a possibility which could be applied to thin films process..