• Title/Summary/Keyword: B 계통

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Variation of Vitexin and Isovitexin Contents in Mungbean (Vigna radiata (L.) Wilczek) Germplasms (녹두 유전자원의 Vitexin 및 Isovitexin 함량 변이)

  • Kim, Dong-Kwan;Son, Dong-Mo;Chon, Sang-Uk;Lee, Kyung-Dong;Rim, Yo-Sup
    • Korean Journal of Plant Resources
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    • v.22 no.2
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    • pp.128-135
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    • 2009
  • In this study, the selected 789 lines having agronomic values out of over 2,500 mungbean (Vigna radiata (L.) Wilczek) germplasms, examined the 27 characters including hypocotyl color and leaf size of 401 lines in 2005 and of 388 lines in 2006, and analyzed the contents of vitexin and isovitexin in these mungbean germplasms. The average contents of vitexin and isovitexin in mungbean of the 401 lines used in 2005 were 8.71($1.1{\sim}13.4$) and 9.54($0.9{\sim}15.9$) mg/g, respectively, and the correlation coefficiency ($R^2$) of the contents of vitexin and isovitexin was 0.958. Nine lines including VC3890B were selected to be the ones containing high vitexin and isovitexin. The average contents of vitexin and isovitexin in mungbean of the 388 lines used in 2006 were 10.17($2.0{\sim}15.9$) and 10.64($0.2{\sim}17.6$) mg/g, respectively, and the correlation coefficiency ($R^2$) of the contents of vitexin and isovitexin was 0.958. Six lines including VC4096-2B-4-B-2-B were selected to be the ones containing high vitexin and isovitexin. The contents of vitexin and isovitexin were higher in the mungbean with larger leaves and longer ripening period out of the main characters.

P Element-Mediated Transformation with the rosy Gene in Drosophila melanogaster (D. melanogaster에 있어서 P Element를 이용한 rosy 유전자의 형질전환)

  • Kim, Wook;Kidwell, Margaret G.
    • The Korean Journal of Zoology
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    • v.38 no.3
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    • pp.340-347
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    • 1995
  • We have used two kinds of P element constructs, Pc[(ry+)B] and p[(ry+)$\Delta$SX9], for genetic transformation by microinjection of D. melanogaster. Pc[(ry+)B] construct carrying the rosy gene within an autonomous P element was injected into a true M strain caring the ry506. mutation. The source of transposase for microinjection and transformation was provided by a P element helper plasmid designated p-$\Delta$2-3hs$\pi$, which was co-injected with nonautonomous P[(ry+)$\Delta$SX9] construct into same ry506 M strains. A dechorination method was adopted and 35 independent transformed lines were obtained froin 1143 G0 Injected (35/1143). About 20% of the injected embryos eclosed as adults. Among G0 eclosed flies, approximately 40% exhibited eye color that was similar to wild-type (ry+), but about 60% of fertile G0 transformed lines appeared to have no G1 transformants. Therefore it is unlikely that G0 expression requires integration of the rosy transposon into chromosomes. Pc[(ry+)B] and P[(ry+)$\Delta$SX9] constructs were found to be nearly same in the frequency of element-mediated transformation. On the basis of these results, nonautonomous P elements constructs could he used as same effective vectors in P element-mediated transformation for introducing and fixing genes in insect populations.

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Immunocytochemical Studies on the Pancreatic Endocrine Cells of Phylogenetically different Vertebrates I. Mammalia and Aves (계통발생에 따른 각종 척추동물의 췌장 내분비세포에 관한 면역세포화학적 연구 1. 포유강 및 조강에 관하여)

  • 김남중;권혁방최월봉
    • The Korean Journal of Zoology
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    • v.34 no.3
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    • pp.352-367
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    • 1991
  • 척추동물 11목 21종의 췌장에서 insulin(B)세포, glucagon(A)세포, somatostatin( D)세포 및 pancreatic polypeptide(PP)세포 등을 면역세포화학적 방법으로 동정하여 이들의 출현율, 분포양상 및 형띨 등을 계통별로 비교하였다. 포유강에서는 전형적인 췌도를 형성하였고, 조강에서는 대 ·소췌도로 구분되었다. 내분비세포의 크기는 계통간의 차이가 있어서 B세포와 PP세포는 포유강의 것이 조강의 것에 비해 윤으나, A세포와 D세포는 그 반대이었다. 세포의 모양은 B세포의 경우 두 강에서 대체로 원형 및 난원형이었고, A세포와 D세포는 원형, 난원형 및 방추형이었다. PP세포는 방추형 및 난원형이 대다수 이었으며 간혹 원형 또는 다각형 등의 모습도 나타났다. 세포들의 출현율은 두 강에서 모두 B세포가가장 많았고, A, D및 PP세포 순으로 낮아졌다. B세포는포유강이 조강보다높았고, A, D및 PP세포는조강이 포유강보다 많았다. B세포는 조강의 경우 대췌도 주변부와 소췌도 중앙부에 분포하였고, 포유강에서는 중앙부에 균등하게 분포하였다. A와 0세포는 포유강에서 주로 췌도 주면부에 분포하나, 조강에서는 췌도중앙부에 위치하였다. PP세포는 두 강에서 대부분 췌도 주변부에 위치하였다. 일반적으로 두강에서 모두 외분비 선포조직에서도 내분비세포들이 출현하였으며 일부 종에서는 췌관상피에서도 내분비세포들이 드물게 나타났다.

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Phenotypic and Transcriptomic Analysis of Nicotiana benthamiana Expressing Cucumber mosaic virus 2b gene (오이모자이크바이러스 2b 유전자 발현 담배의 형태 및 전사체 분석)

  • Sohn, Seong-Han;Kim, Yoon-Hee;Ahn, Yul-Kyun;Kim, Do-Sun;Won, So-Yoon;Kim, Jung-Sun;Choi, Hong-Soo
    • Research in Plant Disease
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    • v.21 no.3
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    • pp.186-192
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    • 2015
  • Cucumber mosaic virus possesses 2b gene known as a suppressor of post-transcriptional gene silencing (PTGS). To investigate its function and effect in plant, transgenic Nicotiana benethamiana expressing 2b gene was developed and analyzed in phenotypic characteristics and differential gene expression (DEG) comparing with wild-type. Eight lines of transgenic plants ($T_0$) were obtained with difficulty and showed severe deformed phenotypes in leaves, flowers, petioles and etc. Moreover, transgenic plants were hardly able to set seeds, but small amounts of seeds were barely produced in some of transgene-hemizygous plants. DEG analysis showed that transgenic plant ectopically accumulated diverse RNA transcripts at higher levels than wild-type probably due to the disturbance in RNA metabolism, especially of RNA decay, caused by 2b-mediated inhibition of PTGS. These ectopic accumulations of RNAs disrupt protein and RNA homeostasis and then subsequently lead to abnormal phenotypes of transgenic plants.

Selection of Heat-tolerant Potato Clones by In Vitro Tuberization from True Potato Seeds (감자 眞正種子의 器內小塊莖 形成에 의한 耐暑性 系統 選拔)

  • 김현준;김영화;유승렬;김병현;김정간
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.2
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    • pp.77-81
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    • 1997
  • For the early selection of heat-tolerant clones, the true potato seeds of 750 clones were obtained by several cross combinations : $CIP\;575015\;{\times}\;katahdin,\;CIP\;575015\;{\times}\;B6603-6,\;84\;I\;35-4\;{\times}\;katahdin,\;CIP\;575015\;{\times}\;NookSack,\;and\;CIP575015\;{\times}\;Superior.$ The ratios of in vitro tuberization at 3$0^{\circ}C$ were decreased by 43% in all cross combinations compared with at 2$0^{\circ}C$. In particular, tuberization rate of $CIP575015\;{\times}\;katahdin$ cross at 3$0^{\circ}C$ was only 21%. On the other hand, the rate of tuberization of $CIP575015\;{\times}B6603-6$ cross was 58 % at 3$0^{\circ}C$, so this cross combination was thought to be good heat-tolerant clone. To screen the heat-tolerant clones in lower land during high temperature period, microtubers were cultivated in Kangnung, and the characters of tubers and productivity were examined. Among screened clones, one clone at 2$0^{\circ}C$ and three clones at 3$0^{\circ}C$ were shown to be heat-tolerant. The yield of 89ML75-8 was 88% more than that of Superior, and dry weight rate of 89 ML64-11 was 18.8%. Therefore, 89ML64-11 clone was considered as a good cultivar for processing.

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A Novel UPFC for Power Transmission System without Series Injection Transformers (송전계통에 적용 가능한 직렬주입변압기가 없는 새로운 UPFC)

  • Han, B.M.;Baek, S.T.;Lee, K.B.
    • Proceedings of the KIEE Conference
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    • 2002.11b
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    • pp.32-34
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    • 2002
  • 본 논문에서는 직렬주업변압기가 없는 새로운 UPFC의 시뮬레이션모형을 개발하고 그 특성을 분석한 내용에 대해 기술하고 있다 송전계통에 적용 가능한 UPFC의 새로운 토폴로지를 제안하고 상세모델을 통하여 계통의 전력조류제어 및 과도상태시의 동특성을 시뮬레이션 한다. 전력시스템의 과도상태 및 제어에 대한 시간영역 시뮬레이션 프로그램인 EMTDC (Electro-Magnetic Transients in DC system)를 사용하여 계통의 전력조류제어인 유효전력과 무효전력의 제어를 수행하고 병렬측의 모선단 입력전압의 크기를 제어한다. 시뮬레이션 결과를 통하여 UPFC 상세모델의 제어성능을 검증한다.

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A Study on development of DFR TimeSync Simulator using IRIG-B signal of GPS (GPS의 IRIG-B 신호를 이용한 DFR TimeSync 시뮬레이터 개발에 관한 연구)

  • Kwak, Sung-Hwan;Kwon, Dae-Yun;Kim, Seog-Joo
    • Proceedings of the KIEE Conference
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    • 2011.07a
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    • pp.737-738
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    • 2011
  • 전력 계통현상 분석장치(DFR)에서 시각동기에 대한 중요성이 부각됨에 따라 장치의 시각동기가 중요하게 대두되고 있다. 본 연구내용에서 보여주고자 하는 DFR TimeSync Simulator는 GPS 수신기로부터 IRIG-B 신호와 1PPS 신호를 수신하여 계통현상 분석장치의 사고발생 이벤트를 위한 절대시각을 제공하는 장치이다. 이 절대시각은 계통현상 분석장치의 정밀성 및 품질을 검증하는데 적용한다. 그리고 개발된 Simulator가 정확한 1PPS 신호를 산출해 내는지를 검증하기 위해 GPS 수신기의 1PPS 신호와 Simulator에서 출력되는 1PPS 신호의 파형을 비교 실험하여 정확도 및 신뢰성을 확인하였다.

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Analysis of fuel cell system connected with Grid (배전계통에서 연료전지시스템의 연계성능 연구)

  • Choi, Jung-Muk;Han, D.H.;Lee, Y.J.;Jung, B.H.;Choe, Gyu-Ha
    • Proceedings of the KIPE Conference
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    • 2010.07a
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    • pp.389-390
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    • 2010
  • 본 논문은 연료전지용 PCS를 계통에 연계하는 과정을 보다 심도있게 진행하기위하여 배전계통을 모의 시뮬레이터로 구현하였다. 제작된 시뮬레이터를 바탕으로 각배전반의 전력품질과 다수 PCS와 계통과의 전력분배를 실험한다.

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Studies on the Performance of Korean Native Chickens II. A Comparison of Performance of Various Korean Native Chickens (한국재래닭의 능력에 관한 연구 II. 한국재래닭의 계통별 능력 비교)

  • 김상호;이상진;강보석;최철환;장병귀;오봉국
    • Korean Journal of Poultry Science
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    • v.25 no.4
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    • pp.177-183
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    • 1998
  • A feeding trial was conducted to investigate the performance of various Korean Native Chickens (KNC) for 64 weeks. Eight hundreds and ten KNC one-day birds were arranged to three strains, Dark Brown(D), Light Brown(L) and Black(B). Each strain had three replicates of 90 birds a replicate. Birds fed same diet with Korean standard feeding. Data were obtained growing performance to 20 week of age and laying performance from twenty one to sixty four week. During the growing stage from hatch to 20 week of age, there were not significant difference to three strains on viability, body weight and feed intake. L strain tended to be earlier 50% egg production day than that of other strains, but was not significantly different. Egg production of L strain was the highest of strains. It showed significantly higher egg production compared to that of D strain(P<0.05), but was not statistically different from that of B strain. Laying peak period was between 28 to 32 week of age in all strains, Egg weight was heavier in D strain(P<0.05). Feed intake was the same as 105g, and feed conversion improved in L strain(P<0.05). There were not difference on interior egg and eggshell quality by strains although eggshell thickness improved slightly in D strain. Fertility of D strain was the highest compared to others(P<0.05) regardless laying stages. Hatchability was not influenced by strains at 39 weeks old, but was significantly higher in B strain at 62 week of age(P< 0.05). The results of this experiment indicated that KNC 3 strains were not effect on egg production, feed intake and feed conversion ratio.

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Molecular Phylogeny of the Family Strigidae (Aves) Based on Mitochondrial Cytochrome b Gene (Mitochondrial Cytochrome b 유전자에 의한 올빼미과 (Family Strigidae)의 분자계통)

  • 류시현;박희천
    • Animal Systematics, Evolution and Diversity
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    • v.19 no.2
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    • pp.297-304
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    • 2003
  • Phylogenetic analysis of 31 species representing 12 genera in the family Strigidae (Aves: Strigiformes) including 5 species (Bubo bubo, Otus sunia, O. semitorques, Ninox scutulato, Strix aluco) collected from Korea has been undertaken using nucleotide sequences of the mitochondrial cytochrome b gene. Maximum likelihood analysis was performed and pairwise genetic distances were calculated with Kimura's two-parameter and p-distance. Among well-aligned 959 bp used for this study, 459 sites were variable and 398 sites were informative for the phylogenetic analysis. The family Strigidae was divided into three subgroups, Clade I (Aegolius), Clade II (Athene, Micrathene, Glaucidium and Surnia) and Clade III (Bubo, Nycteo, Pulsatrix, Strix, Otus, Ptilopsis, and Ninox). Also, two separated subgroups in the genus Otus were confirmed by the geographical distribution.