• BMB Reports
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• 제40권2호
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• pp.218-225
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• 2007

In Arabidopsis thaliana, the microtubule-associated protein AtMAP65-1 shows various functions on microtubule dynamics and organizations. However, it is still an open question about whether AtMAP65-1 binds to tubulin dimers and how it regulates microtubule dynamics. In present study, the tubulin-binding activity of AtMAP65-1 was investigated. Pull-down and co-sedimentation exp eriments demonstrated that AtMAP65-1 bound to tubulin dimers,at a molar ratio of 1 : 1. Cross-linking experiments showed that AtMAP65-1 bound to tubulin dimers by interacting with $\alpha$-tubulin of the tubulin heterodimer. Interfering the bundling effect of AtMAP65-1 by addition of salt and monitoring the tubulin assembly, the experiment results indicated that AtMAP65-1 promoted tubulin assembly by interacting with tubulin dimers. In addition, five truncated versions of AtMAP65-1, namely AtMAP65-1 $\Delta$N339 (amino acids 340-587); AtMAP65-1 $\Delta$N494 (amino acids 495-587); AtMAP65-1 340-494 (amino acids 340-494); AtMAP65-1 $\Delta$C495 (amino acids 1-494) and AtMAP65-1 $\Delta$C340 (amino acids 1-339), were tested for their binding activities and roles in tubulin polymerization in vitro. Four (AtMAP65-1 $\Delta$N339, $\Delta$N494, AtMAP65-1 340-494 and $\Delta$C495) from the five truncated proteins were able to co-sediment with microtubules, and three (AtMAP65-1 $\Delta$N339, $\Delta$N494 and AtMAP65-1 340-494) of them could bind to tubulin dimers in vitro. Among the three truncated proteins, AtMAP65-1 $\Delta$N339 showed the greatest activity to promote tubulin polymerization, AtMAP65-1 $\Delta$N494 exhibited almost the same activity as the full length protein in promoting tubulin assembly, and AtMAP65-1 340-494 had minor activity to promote tubulin assembly. On the contrast, AtMAP65-1 $\Delta$C495, which bound to microtubules but not to tubulin dimers, did not affect tubulin assembly. Our study suggested that AtMAP65-1 might promote tubulin assembly by binding to tubulin dimers in vivo.

• 한국의학물리학회지:의학물리
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• 제25권2호
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• pp.65-71
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• 2014

A Varian Portal Dosimetry system was compared to an isocentrically mounted MapCHECK 2 diode array for volumetric modulated arc therapy (VMAT) QA. A Varian TrueBeam STx with an aS-1000 digital imaging panel was used to acquire VMAT QA images for 13 plans using four photon energies (6, 8, 10 and 15 MV). The EPID-based QA images were compared to the Portal Dose Image Prediction calculated in the Varian Eclipse treatment planning system (TPS). An isocentrically mounted Sun Nuclear MapCHECK 2 diode array with 5 cm water-equivalent buildup was also used for the VMAT QAs and the measurements were compared to a composite dose plane from the Eclipse TPS. A ${\gamma}$ test was implemented in the Sun Nuclear Patient software with 10% threshold and absolute comparison at 1%/1 mm (dose difference/distance-to-agreement), 2%/2 mm, and 3%/3 mm criteria for both QA methods. The two-tailed paired Student's t-test was employed to analyze the statistical significance at 95% confidence level. The average ${\gamma}$ passing rates were greater than 95% at 3%/3 mm using both methods for all four energies. The differences in the average passing rates between the two methods were within 1.7% and 1.6% of each other when analyzed at 2%/2 mm and 3%/3 mm, respectively. The EPID passing rates were somewhat better than the MapCHECK 2 when analyzed at 1%/1 mm; the difference was lower for 8 MV and 10 MV. However, the differences were not statistically significant for all criteria and energies (p-values >0.05). The EPID-based QA showed large off-axis over-response and dependence of ${\gamma}$ passing rate on energy, while the MapCHECK 2 was susceptible to the MLC tongue-and-groove effect. The two fluence-based QA techniques can be an alternative tool of VMAT QA to each other, if the limitations of each QA method (mechanical sag, detector response, and detector alignment) are carefully considered.

• 한국식품저장유통학회지
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• 제6권1호
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• pp.43-47
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• 1999

To develop a wrapping film, which suppresses the microbial decay through the storage and distribute of greenhouse fresh produce, the antimicrobial packaging films were made and applied to the preservation of grapes(Campbell early). For the purpose the films were made by adding 1% grapefruit seed extract(GFSE) to LDPE film(Control). Graps were separately wrapped with packaging films in the state of closely-adhered packaging as well as modified atmosphere packaging(MAP). The wrapped grapes were stored at 5$^{\circ}C$ for 65 days and then the colony count of contaminated microorganims, decay ration of grapes, the gas component within the packages and chemical qualities were investigated. The antimicrobial film packaging showed the efficient results to suppress microbial growth as compared with control. The total number of containated microorganisms were decreased gradually through all the storage period. In the closely-adhered packaging and MAP the decay ratios of grapes was 31% and 19%, indivisually. After the storage period of 65 days, the interior gas components of MAP were 4.5% of O2 and 17.6% of CO2, which were efficient for the storage of grapes. In addition, no negative effects in sweetness and acidities occurred.

• The Korean Journal of Ecology
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• 제19권5호
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• pp.403-416
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• 1996

Global natural vegetation mapping (GNVM) system was developed for estimating potential forest area of the globe. With input of monthly mean temperature and monthly precipitation observed at weather stations, the system spherically interpolates them into 1°×1°grid points on a blobe, converts them into vegetation types, and produces a potential vegetation map and a potenital vegetation area. The spherical interpolation was based on negative exponential function fed from the constant radius stations with oval weighing method which is latitudinally elongated weighing in temperature and longitudinally elongated weighing in precipitation. The temperature values were corrected for altitude by applying a linear lapse-rate (0.65℃ / 100m) with reference to a built-in digital terrain map of the globe. The vegetation classification was based upon Koppen’s sKDICe. The potential forest area is estimated for 6.96 Gha (46.24％) of the global land area (15.05 Gha).

• 한국임상수의학회지
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• 제21권2호
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• pp.168-171
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• 2004

The effects of alterations of dose of xylaznie (X) and Zoltil$\circledR$ (TZ) on canine anesthesia were examined. Experimental groups were divided into three (Group 1: X 1.1 mg/kg and TZ 10 mg/kg, Group 2: X 1.65 mg/kg and TZ 7.5 mg/kg, Group 3: X 2.2 mg/kg and TZ 5 mg/kg), and each had 5 dogs. A femoral artery was catheterized for measurement of blood pressure, and baseline value was measured. The dogs were sedated with xylazine intramuscularly, then after 10 minutes TZ were injected intravenously. Mean arterial blood pressures (MAP), duration of analgesia, mean arousal time (MAT) and mean walking time (MWT) after TZ injection were measured, and the depth of analgesia and the quality of recovery were scored. The values of MAP were recorded from the time of pre-xylazine injection to arousal. Duration of analgesia and was assessed by tail clamping test, and which were done at 10 minutes intervals after TZ injection. The decreases of MAP from 40 minutes after TZ injection were significant (p<0.05). In group 2, MAP at 20 minutes, and from 40 minutes to arousal were significantly decreased (p<0.05). In group 3, MAP were significantly decreased from 40 minutes. MAT were 62.2$\pm$9.2 minutes in group 1, 60.2$\pm$7.5 minutes in group 2, and 71.0$\pm$6.9 minutes in group 3. MAT in group 3 was significantly increased compared with group 2 (p<0.05), and the differences of MWT among each groups were not significant (p>0.05). The scores of quality of recovery were significantly lowered in group 3 compared with group 1 or group 2, which means the side effects of recovery were less occurred. Thus, it was considered that the combination X 2.2 mg/kg IM and TZ 5 mg/kg IV is more effective to surgical procedures and to prevent long and rough recovery of Zoletil anesthesia.

• 대한공간정보학회지
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• 제16권1호
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• pp.57-65
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• 2008

본 연구에서는 시 군 구 경계지역의 지적도면 전산화 데이터와 현황을 참조할 수 있는 데이터를 이용하여 대상 지역에서의 지적불부합지 실태를 분석하여 봄으로써 향후 행정경계 지역에서의 지적불부합지 정리를 위한 기초자료를 제공하고자 하였다. 조사지역의 지적불부합 실태를 조사한 결과, 행정경계 주변지역의 지적불부합 정도는 X좌표의 RMSE가 ${\pm}3.15m$, Y좌표의 RMSE가 ${\pm}2.85m$로 산출되었고, 행정경계 비주변 지역은 X좌표의 RMSE가 ${\pm}1.33m$, Y좌표의 RMSE가 ${\pm}0.75m$로 산출되어 행정구역 경계지역에서 지적불부합지가 많이 발생되고 있음을 확인 할 수 있었다.

• 대한방사성의약품학회지
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• 제3권2호
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• pp.65-71
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• 2017

To assess the effects of filter and reconstruction of Cu-64 PET data on Siemens scanner, the various reconstruction algorithm with various filters were assessed in terms of spatial resolution, non-uniformity (NU), recovery coefficient (RC), and spillover ratio (SOR). Image reconstruction was performed using filtered backprojection (FBP), 2D ordered subset expectation maximization (OSEM), 3D reprojection algorithm (3DRP), and maximum a posteriori algorithms (MAP). For the FBP reconstruction, ramp, butterworth, hamming, hanning, or parzen filters were used. Attenuation or scatter correction were performed to assess the effect of attenuation and scatter correction. Regarding spatial resolution, highest achievable volumetric resolution was $3.08mm^3$ at the center of FOV when MAP (${\beta}=0.1$) reconstruction method was used. SOR was below 4% for FBP when ramp, Hamming, Hanning, or Shepp-logan filter were used. The lowest NU (highest uniform) after attenuation & scatter correction was 5.39% when FBP (parzen filter) was used. Regarding RC, 0.9 < RC < 1.1 was obtained when OSEM (iteration: 10) was used when attenuation and scatter correction were applied. In this study, image quality of Cu-64 on Siemens Inveon PET was investigated. This data will helpful for the quantification of Cu-64 PET data.

• Journal of Periodontal and Implant Science
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• 제48권5호
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• pp.305-316
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• 2018

Purpose: The aim of the present study was to evaluate the biocompatibility and barrier function of mussel adhesive protein (MAP)-loaded collagen membranes in guided bone regeneration (GBR). Methods: Eight male New Zealand white rabbits were used. Four circular defects (diameter: 8 mm) were created in the calvarium of each animal. The defects were randomly assigned to 1) a negative control group, 2) a cyanoacrylate (CA)-loaded collagen membrane group (the CA group), 3) a MAP-loaded collagen membrane group (the MAP group), and 4) a group that received a polycaprolactone block with MAP-loaded collagen membrane (the MAP-PCL group). Specimens were harvested at 2 weeks (n=4) and 8 weeks (n=4) postoperatively for observational histology and histometric analysis. Results: In the histologic analysis, MAP was completely absorbed without any byproducts. In contrast, some of the CA adhesive remained, showing an inflammatory reaction, at 8 weeks. In the MAP-PCL group, the MAP-loaded collagen membranes served as a barrier membrane despite their fast degradation in GBR. No significant difference was found in the amount of new bone between the MAP-PCL and MAP groups ($1.82{\pm}0.86mm^2$ and $2.60{\pm}0.65mm^2$, respectively). Conclusions: The MAP-loaded collagen membrane functioned efficiently in this rabbit calvarial GBR model, with excellent biocompatibility. Further research is needed to assess clinical applications in defect types that are more challenging for GBR than those used in the current model.

• 대한원격탐사학회지
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• 제26권2호
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• pp.87-98
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• 2010

한반도 전역과 같은 상대적으로 넓은 지역의 정확한 분류를 위해서는 단일 영상 분류 후 영상정합 방식보다는 영상 정합 후 분류방법이 보다 정확하다. 또한 다중시기 정보는 분류에 매우 유용하게 사용될 수 있다. 본 연구에서는 한반도 전역을 대상으로 최적의 Landsat ETM+ 영상정합 방식을 제시하였다. 한반도 전역에 대해 2000년부터 2001년까지 획득된 총 65개의 Landsat ETM+영상을 이용하여 낙엽기, 이앙기, 개엽기 각각 정합 영상을 제작하였다. 이때 보다 정확한 영상정합을 위해 히스토그램 매칭, 중앙영상을 기준으로 한 1차 회귀식적용방법, Landsat 촬영 패스별로 적용한 1차 회귀식 적용방법, 총 세 가지 상대복사보정 방법을 적용하였다. 적용 결과, 패스별 상대복사보정한 결과가 그 보정 효과가 크면서, 높은 분류 정확도를 나타냈다. 또한 시기별 정합영상을 살펴보면, 개엽기의 정합영상이 타시기에 비해 상대적으로 인접한 영상 간 지표물의 변이가 다양하게 나타나는 것을 볼 수 있었다.

• 한국음향학회지
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• 제16권2호
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• pp.58-65
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• 1997

본 논문에서는 시계열 image data를 안정되고 높은 정확도로 분류할 수 있는 자동분류법을 제안하였다. 제안한 방법은 대상 영역에 관한 분류도가 기존재하던 가, 아니면 최소한 시계열 image data 중 어느 한 image data가 분류되어 있다고 하는 전제조건에 그 기초를 두고 있다. 분류도는 training area를 선정하기 위라여 사용하는 기준주제도로 사용되어진다. 제안한 방법은 1)기준주제도를 사용한 training data의 추출, 2)taining data의 균질성에 의거한 변화화소의 검출, 3)검출된 변화화소에 대한 clustering, 4)training data의 재구성, 5)maximum likelihood classifier와 같은 판별법에 의한 분류 등 5개의 단계로 구성된다. 제안한 방법의 성능을 정량적으로 평가하기 위하여 4개의 시계열 Landsat TM image data를 제안한 방법과 숙련된 operator가 필요한 기존의 방법으로 각각 분류하여 비교 검토하였다. 그 결과, 기존의 방법으로는 숙련된 operator가 필요하고, 분류도를 얻기까지 수일이 소요되는 데 반하여, 제안한 방법으로는 숙련된 operator 없이, 신뢰성 있는 분류도를 수 시간 내에 자동으로 얻을 수 있었다.