• Korean Journal of Agricultural Science
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• v.19 no.1
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• pp.1-8
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• 1992

This experiment was conducted to improve the germination when we sowed Chinese milk vetch (Astragalus sinicus L. Local vas. Jinjoo) in the up-land.. The results are summerized as follows 1. There is no difference on the germination of Chinese milk vetch from $15^{\circ}C$ to $35^{\circ}C$. Germination ratio is 7l%, speed is 60% and mean of germinating day. is about 4. But at $10^{\circ}C$ germination ratio and speed are decreased as 65%, 8% but mean of germinating day is increased as 8. 2. Chemicals used for improve of germination $KNO_3$, $H_2SO_4$, HCl, NaOCl, NaOH and GA were increased about 6~8% of germination ratio. 3. Germination in response to soil covering up depth, surface sowing showed 68% of germination and not more than 4cm the seed is germinated 60% but not less than 6cm, germination ratio is not more than 50%. 4. Soil water content needed in the germination is proper about 70~80% of field water capacity and the ratio is 65% but not. more than 40% germination ratio is not more than 31%. 5. Germination in response to seed treatment (1 day sbsorption /1 day dry) germination ratio is not decreased when it treated 2 times but not less than 3 times it decreased seriously and not less that: 5 times it decreased not more than 31%. 6. Germination in response to seed largement, small seed is not good for germination, so seeds harvested after the majority of the seed is ripened.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.1
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• pp.49-54
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• 1999

An experiment was carried out using pots to investigate the effects of Chinese milk vetch on the seedling establishment and growth in rice. Four irrigations with five-day intervals and three different levels of vetch straw were investigated. Significantly higher panicle numbers were obtained in vetch-treated pots. Vetch levels were non-treatment as checks, vetch with top removed, root plus shoot 7.5 ton/ha, and root plus shoot 3.0 ton/ha. The time for complete degradation of vetch straws was reduced from 10 days to 5 days as submerged time was delayed, and was affected by the amount of mulched vetch straws. As the mulched vetch amount increased, the time for a complete degradation was extended from 4 days to 12 days. Grain yield and its components were significantly affected by irrigation time and mulched vetch amount. Effectively controlled, lowered reduction damage from the degrading vetch straw, irrigation date and vetch amount were the most important factors for the improving of seedling establishment in direct-sown rice.

• Proceedings of the Korean Society of Crop Science Conference
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• 2007.11a
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• pp.243.2-243.2
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• 2007

• Korean Journal of Microbiology
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• v.3 no.2
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• pp.22-26
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• 1965

In order to investigate the host range of the mosaic disease of black locust in the Chunchon area, the sap of the mosaic-diseased leaves of black locust itself and the cowpea leaves infected with the above mentioned sap, were inoculated to 53 species of plants belong to 12 families. As to the result, no difference in infection was found as related to the virus sources, and the infection was recognized in 4 species of the family Chenopodiaceae and 8 species of the family Leguminosae. The plants recognized as hosts are as follows: the plants which showed local infection are Chenopodium album, Ch. ambrosioides, Ch. quinoa; the plants which showed systemic infection are Chenopodium amaranticolor, Phaseolus vulgaris, Robinia pseudo-acacia, Vigna sinensis; and Astragalus sinicus, Melilotus indicus, Phaseolus angularis, Pisum sativum and Vicia faba were recognized as carriers. Through investigating its host ranges and symptoms, this mosaic virus of black locust seems not to be regarded as the group of the black locust mosaic virus in southeastern Europe reported by Milinko et al (1961). And, too, it is thought hardly to exist in combination with the cowpea mosaic virus. It appears, therefore, that this mosaic virus was confined to that of black locust.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.4
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• pp.322-328
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• 2006

A series of methanol extracts from leaf and root parts in spring- and winter-barley plants were assayed to determine their allelopathy and antioxidant activity. The methanol extracts applied on filter paper in a Petri-dish significantly inhibited root growth of Chinese milkvetch (Astragalus sinicus L.) seedlings. Leaf extracts at 25 and 50 g $L^{-1}$ inhibited root growth of Chinese milkvetch seedlings more than root extracts. No difference in phytotoxic effects of spring- and winter-barley seedlings extracts on root growth of Chinese milkvetch was observed. Methanol extracts dose-dependently increased DPPH free radical scavenging activity in vitro. DPPH free radical scavenging activity was higher in the methanol extracts from winter-barley seedlings than in those from spring-barley seedlings, and from leaf extracts than from root extracts. The antioxidant potential of the individual fraction from the methanol extracts of spring-barley seedlings was in order of n-butanol>ethyl acetate>water>chloroform>n-hexane fraction. By means of HPLC analysis, spring-barley (200.62 mg $100g^{-1}$) had more amount of total phenol acid than winter-barley (114.08 mg $100g^{-1}$). Especially, ferulic acid was detected in spring-barley extract (183.46 mg $100g^{-1}$) as the greatest amount. These results suggest that early seedlings of barley plants had potent allelopathy and antioxidant activity, and their activities were differently exhibited depending on plant parts and growing condition.

• Journal of Food Hygiene and Safety
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• v.29 no.3
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• pp.181-188
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• 2014

The objective of this study was to analyze the microbiological hazards of Astragalus membranaceus Bunge on the post-harvest processing. Samples from processing equipments (cleaner, water, cart, table, tray and packaging machine), personal hygiene (hand) and harvested crops (before washing, after washing, after sorting, and after drying) were collected from four farms (A, B, C, and D) located in Chungchengbuk-do, Korea. The samples were analyzed for sanitary indication bacteria and pathogenic bacteria. First, total aerobic bacteria and coliform in processing facilities were detected at the levels of 0.93~4.86 and 0.33~2.28 log CFU/$100cm^2$ and/mL respectively. In particular, microbial contamination in hand (5.43~6.11 and 2.52~4.12 log CFU/Hand) showed higher than processing equipments. Among the pathogenic bacteria, Bacillus cereus was detected at the levels of 0.33~2.41 log CFU/$100cm^2$, 1.48~3.27 log CFU/Hand and 0.67~3.65 log CFU/g in equipments, hands, and plants and Staphylococcus aureus were detected in cleaner, table, hand and harvested crops (before washing and after sorting) by qualitative test. Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella spp. were not detected. These results indicated that personal hygiene and processing equipments should be managed to reduce the microbial contamination of A. membranaceus Bunge. Therefore, management system such as good agricultural practices (GAP) criteria is needed for hygienic agricultural products.

• Journal of Plant Biotechnology
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• v.41 no.2
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• pp.100-106
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• 2014

In order to develop an efficient in vitro micropropagation technique for a rare plant species, Astragalus membranaceus Bunge var. alpinus N., shoot proliferation and in vitro or in vivo rootings were conducted and hyperhydrated leaf generated from cultures was histologically observed. During shoot induction, no distinct effect on multiple shoot induction was found between BA and kinetin treatment. BA enhanced the number of internodes, whereas kinetin stimulated shoot elongation. Hyperhydrated leaf composed of bigger cells and retarded palisade parenchyma and showed irregular cell arrangement compared to normal leaf. Especially starch content in hyperhydrated leaf was significantly reduced. The best rooting rate was achieved by B5 medium among three different medium (B5, MS and WPM) and 0.1mg/L IBA treatment induced the highest rooting ratio (80%). No statistical difference was induced by explant types (apical bud or axillary bud) in terms of rooting ratio. In vivo cutting induced rooting rate up to 65% by 0.5% IBA/Talc powder treatment. Although in vivo rooting rate was less efficient compared to in vitro rooting, better survival rate was observed after soil acclimatization. Present study suggested that above micropropagation techniques can be used for rapid multiplication as well as in vitro or in vivo conservation of the species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.3
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• pp.248-253
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• 1990

Germination and seedling growth of chinese milkyetch (Astragalus sinicus L. Line A) seeds were measured at four temperatures (15. 25. 30, and 35$^{\circ}C$), three polyethylene glycol (PEG) 10.000 (MW) solutions (0, 20 and 25 g/100$m\ell$ H$_2$O), and five sulfuric acid treatments (conc, H$_2$SO$_4$: Seeds/weight base: 0, 1:1 10min.. 1:1 20min.. 1:1.5 10min. and 1:1.5 20min, immersion), Adjustments of PEG 10.000 solutions were based on water potentials of 0.0, -0,5 and -0.8 ㎫ at room temperature. Observations were taken at 7 days for each treatment combinations. Germination percent of chinese mikvetch seeds was the highest at 15$^{\circ}C$ and seeding length was the longest at 25$^{\circ}C$. Both germination percent and seedling length were rapidly decreased as PEG concentration increased from 0 to 25g/100$m\ell$ H$_2$O. The highest germination percent was found at PEG 20 and 25 of 15$^{\circ}C$ among those of four temperatures, Sulfuric acid treatments showed little effect on germination percent and seedling length compared to untreated control, However. in the case of PEG 0, greater germination perccent was obtained at the treatment of 1:1.5 for 10 minutes immersion, The result of this study-indicated that germination of chinese milkvetch seeds was affected mostly by moisture stress conditions.

• Korean journal of applied entomology
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• v.51 no.1
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• pp.67-72
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• 2012

This study was conducted to evaluate the scouting methods for larva and adults of the alfalfa weevil, $Hypera$ $postica$ Gyllenhal on Chinese milkvetch, $Astragalus$ $sinicus$ L. in the field. Three sampling methods, shake-bucket, shake-picking, and a sweeping net were evaluated for collecting alfalfa weevil larvae. We found significant differences among scouting methods and date in all survey fields. Sweep-net sampling was less able to detect small larvae than large larvae, which were not detected until early April whereas the shake-bucket, and shake-picking methods efficiently collected larvae from middle March. A Pitfall trap with three different baits - no bait, kidney bean seeds and sprouting kidney beans were compared for collection efficiency of alfalfa weevil adults. Collection efficiencies were significantly different by bait(p<0.018). Traps baited with sprouting kidney beans were the most efficient for collecting the alfalfa weevil. The number of alfalfa weevil caught was not significantly different between kidney bean seed and no bait in the pitfall trap. Therefore, the shake-bucket method for larvae and a pitfall trap baited with sprouting kidney beans for adults are recommended for scouting of the alfalfa weevil in a Chinese milkvetch field.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.6
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• pp.1103-1106
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• 2011

Optimum application level of liquid pig manure for increasing Astragalus sinicus L. (ASASI) and Lolium multiflorum Lam. (LOLMU) yield was investigated. The green manure crop yield were generally high in the order of ALPM 100 ${\geqq}$ APLM 75 ${\geqq}$ APLM 50 ${\geqq}$ APLM 0 in site 1 (ASASI), and LPLM 100 ${\geqq}$ LPLM 75 > LPLM 50 ${\geqq}$ LPLM 0 in site 2 (LOLMU). The optimum application level of liquid pig manure was 100% of nitrogen fertilization level.