• Title/Summary/Keyword: Assembly sequence

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Study on Analysis Method for Welding Deformation of curved Block - Development of Analysis and Application of real Block (곡 블록 용접변형 해석법에 관한 연구 - 해석법 정립 및 실 블록 적용)

  • Lee Myeong-Su;Jang Gyeong-Bok;Yang Jin-Hyeok;Gang Seong-Su
    • Proceedings of the KWS Conference
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    • 2006.05a
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    • pp.77-79
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    • 2006
  • To achieve high productivity of assembly hull blocks, it is important to predict welding deformations accurately and to apply these data to the production planning. In the deformation analysis of hull block, simplified methods (elastic analysis) such as inherent method, equivalent loading method and local & global approach are usually used instead of thermal-elastic-plastic analysis because of calculating time and cost. To be much more practical, these simplified methods should consider gravity effect of plate and contact condition between the plate and the positioning jig. In this research, using finite element method, practical predicting method for the welding deformation of the curved hull blocks with considering welding sequence, gravity effect and contact condition is proposed.

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Identification of a Cellular Protein Interacting with Murine Retrovirus Gag Polyproteins

  • Choi, Wonja
    • Journal of Microbiology
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    • v.34 no.4
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    • pp.311-315
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    • 1996
  • The retroviral Gag polyprotein directs the assembly of virion particles and plays an important role in some events after entry into a host cell. The Gag polyprotein of a virus mixture is responsible for inducing murine acquired immunodeficiency syndrome (MAIDS) when injected into susceptible strains of mice. In order to identify the host cellular proteins which interact with the MAIDS virus Gag proteins and possibly mediate the function of the Gag proteins, mouse T-cell leukemic cDNA expression library was screened using the yeast GAL4 two hybrid system. Of 11 individual positive clones, the clone Y1 was selected for the study of protein-protein interaction. Its DNA sequence revealed that it was an exact match to the murine SH3 domain-containing protein SH3P8. It is expressed as 2.4 kbp transcripts in testis at higher levels and in various tissues tested at lower levels. Glutathione S-transferase-Y1 fusion protein binds tightly to $Pr60^{def-gag}$ as well as $Pr65^{eco-gag}$.

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Study on Analysis Method for Welding Deformation of curved Block - Specimen test and Verification (곡 블록 용접변형 해석법에 관한 연구 - 시험편 테스트 및 검증)

  • Lee, Myeong-Su;Jang, Gyeong-Bok;Park, Jung-Gu;Yang, Jin-Hyeok;Gang, Seong-Su
    • Proceedings of the KWS Conference
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    • 2005.11a
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    • pp.17-19
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    • 2005
  • To achieve high productivity of assembly hull blocks, it is important to predict welding deformations accurately and to apply these data to the production planning. In the deformation analysis of hull block, simplified methods (elastic analysis) such as inherent method, equivalent loading method and local & global approach are usually used instead of thermal-elastic-plastic analysis because of calculating time and cost. To be much more practical, these simplified methods should consider gravity effect of plate and contact condition between the plate and the positioning jig. In this research, using finite element method, practical predicting method for the welding deformation of the curved hull blocks with considering welding sequence, gravity effect and contact condition is proposed.

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A Study on the Body Welding Operation Scheduling Considering the Assembly Line's Input Sequence in Construction Equipment Manufacturing (건설기계 조립 라인 투입 순서를 고려한 제관 공정 생산 스케줄링에 관한 연구)

  • Kim, Ki-Dong;Choi, Ho-Sik
    • Journal of Industrial Technology
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    • v.27 no.A
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    • pp.69-76
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    • 2007
  • The body of an excavator, one sort of the construction equipment, consists of mainframe part, track frame part, boom part and arm part. The all parts are manufactured in the body welding operation. The scheduling in the body welding operation of a construction equipment manufacturing is to take all the various constraints into consideration. The offset time, due date, daily capacity of operations, daily jig's capacity, precedence relation, outsourcing, alternative resource and all of the shop floor environment should be considered. An APS(Advanced Planning & Scheduling) system is a proper and efficient system in such circumstance. In this paper, we present an APS system, the optimal scheduling system for the construction equipment manufacturing specifically for the body welding operation, using ILOG Solver/Scheduler. ILOG Solver/Scheduler is a general purposed commercial software which supports to find a feasible or optimal solution using object oriented technique and constraints satisfaction programming, given constraints and objectives.

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SFannotation: A Simple and Fast Protein Function Annotation System

  • Yu, Dong Su;Kim, Byung Kwon
    • Genomics & Informatics
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    • v.12 no.2
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    • pp.76-78
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    • 2014
  • Owing to the generation of vast amounts of sequencing data by using cost-effective, high-throughput sequencing technologies with improved computational approaches, many putative proteins have been discovered after assembly and structural annotation. Putative proteins are typically annotated using a functional annotation system that uses extant databases, but the expansive size of these databases often causes a bottleneck for rapid functional annotation. We developed SFannotation, a simple and fast functional annotation system that rapidly annotates putative proteins against four extant databases, Swiss-Prot, TIGRFAMs, Pfam, and the non-redundant sequence database, by using a best-hit approach with BLASTP and HMMSEARCH.

Prediction of Welding Deformation of Ship Hull Blocks

  • C. D. Jang;Lee, C. H.
    • Journal of Ship and Ocean Technology
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    • v.7 no.4
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    • pp.41-49
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    • 2003
  • Welding deformation reduces the accuracy of ship hull blocks and decreases productivity due to the need for correction work. Preparing an error-minimizing guide at the design stage will lead to higher quality as well as higher productivity. Therefore, developing a precise method to predict the weld deformation is an essential part of it. This paper proposes an efficient method for predicting the weld deformation of complicated structures based on the inherent strain theory combined with the finite element method. A simulation of a stiffened panel confirmed the applicability of this method to simple ship hull blocks.

Development of Analysis Platform for Plant Sequence Assembly (식물 염기 서열 분석 플랫폼의 개발)

  • Choi, Hoon;Um, Jungho;Yoon, Hwa-mook;Choi, Yun-Soo;Lee, Minho;Lee, Won-Goo;Song, Sa-Kwang;Jung, Hanmin
    • Proceedings of the Korea Information Processing Society Conference
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    • 2012.11a
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    • pp.1158-1161
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    • 2012
  • 식물 염기 서열 분석은 동물에 비해 유전체가 길고 데이터 양이 많아 특별한 전략을 요구하고 있다. 본 논문은 FCLA 전략을 지원하기 위한 데이터 분석 플랫폼의 개발에 대해 서술한다.

Complete genome sequence of Bacillus coagulans CACC834 isolated from canine

  • Kim, Jung-Ae;Kim, Dae-Hyuk;Kim, Yangseon
    • Journal of Animal Science and Technology
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    • v.63 no.6
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    • pp.1464-1467
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    • 2021
  • Bacillus coagulans CACC 834 was isolated from canine feces, and its potential probiotic properties were characterized by functional genome analysis. Whole-genome sequencing of B. coagulans CACC 834 was performed using the PacBio RSII platforms. The complete genome assembly consisted of one circular chromosome (3.1 Mb) with guanine (G) + cytosine (C) content of 47.1%. Annotation revealed 3,181 protein-coding sequences (CDSs), 30 rRNAs, and 83 tRNAs. Gene associated 11% of the genes were involved in replication, recombination, and repair. We also annotated various stress-related, acid resistance, bile salt resistance and adhesion-related domains in this strain, which likely provide support in exerting probiotic action by survival under gastrointestinal tract. These results add to our comprehensive understanding of B. coagulans and suggest potential mammal-related industrial applications.

Draft Genome Sequence of Aureobasidium pullulans Strain MHAU2101, a Biological Control Agent against Fire Blight from Korea

  • Lin He;Huan Luo;Mi-Hyun Lee;Jun Myoung Yu
    • Microbiology and Biotechnology Letters
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    • v.51 no.4
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    • pp.538-541
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    • 2023
  • In this study, we present the draft genome of Aureobasidium pullulans strain MHAU2101, which is the first strain to effectively control fire blight caused by Erwinia amylovora in Korea. The genome of strain MHAU2101 was composed of 28,669,322 base pairs, with a C+G content of 50.4%. The assembly comprised 17 contigs and had 99.22% completeness. The results of this study will be a valuable resource for future research on the biocontrol mechanism of A. pullulans strain MHAU2101.

Structural Insights and Mechanistic Understanding of Iron-Molybdenum Cofactor Biosynthesis by NifB in Nitrogenase Assembly Process

  • Wonchull Kang
    • Molecules and Cells
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    • v.46 no.12
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    • pp.736-742
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    • 2023
  • NifB, a radical S-adenosylmethionine (SAM) enzyme, is pivotal in the biosynthesis of the iron-molybdenum cofactor (FeMo-co), commonly referred to as the M-cluster. This cofactor, located within the active site of nitrogenase, is essential for the conversion of dinitrogen (N2) to NH3. Recognized as the most intricate metallocluster in nature, FeMo-co biosynthesis involves multiple proteins and a sequence of steps. Of particular significance, NifB directs the fusion of two [Fe4S4] clusters to assemble the 8Fe core, while also incorporating an interstitial carbide. Although NifB has been extensively studied, its molecular mechanisms remain elusive. In this review, we explore recent structural analyses of NifB and provide a comprehensive overview of the established catalytic mechanisms. We propose prospective directions for future research, emphasizing the relevance to biochemistry, agriculture, and environmental science. The goal of this review is to lay a solid foundation for future endeavors aimed at elucidating the atomic details of FeMo-co biosynthesis.