Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Draft Genome Sequence of Aureobasidium pullulans Strain MHAU2101, a Biological Control Agent against Fire Blight from Korea

  • Lin He (Department of Applied Biology, Chungnam National University) ;
  • Huan Luo (Department of Applied Biology, Chungnam National University) ;
  • Mi-Hyun Lee (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Jun Myoung Yu (Department of Applied Biology, Chungnam National University)
  • Received : 2023.11.07
  • Accepted : 2023.11.28
  • Published : 2023.12.28
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Abstract

In this study, we present the draft genome of Aureobasidium pullulans strain MHAU2101, which is the first strain to effectively control fire blight caused by Erwinia amylovora in Korea. The genome of strain MHAU2101 was composed of 28,669,322 base pairs, with a C+G content of 50.4%. The assembly comprised 17 contigs and had 99.22% completeness. The results of this study will be a valuable resource for future research on the biocontrol mechanism of A. pullulans strain MHAU2101.

Keywords

Acknowledgement

This work was supported by a grant from the Agenda program (PJ015594) of the Rural Development Administration, Republic of Korea.

References

  1. Urzi C, De Leo F, Passo CL, Criseo G. 1999. Intra-specific diversity of Aureobasidium pullulans strains isolated from rocks and other habitats assessed by physiological methods and by random amplified polymorphic DNA (RAPD). J. Microbiol. Methods 36: 95-105.
  2. Chi Z, Wang F, Chi Z, Yue L, Liu G, Zhang T. 2009. Bioproducts from Aureobasidium pullulans, a biotechnologically important yeast. Appl. Microbiol. Biotechnol. 82: 793-804.
  3. Ravella SR, Quinones TS, Retter A, Heiermann M, Amon T, Hobbs PJ. 2010. Extracellular polysaccharide (EPS) production by a novel strain of yeast-like fungus Aureobasidium pullulans. Carbohydr. Polym. 82: 728-732.
  4. Zhang D, Spadaro D, Garibaldi A, Gullino ML. 2010. Efficacy of the antagonist Aureobasidium pullulans PL5 against postharvest pathogens of peach, apple and plum and its modes of action. Biol. Control 54: 172-180.
  5. Kunz S, Zeng Q, Johnson KB. 2023. History, efficacy, orchard ecology, and mode of action of Aureobasidium pullulans, the microbial agent in Blossom Protect, for suppression of fire blight of pome fruit. J. Plant. Pathol. https://doi.org/10.1007/s42161-023-01448-4.
  6. Oh H, Choi H-W, Lee YH, Oh S-K. 2022. Inhibitory effects of Aureobasidium pullulans MHAU2101 isolated from domestic pear blossom against fire blight. Korean J. Mycol. 50: 332.
  7. Walker BJ, Abeel T, Shea T, Priest M, Abouelliel A, Sakthikumar S, et al. 2014. Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS One 9: e112963.
  8. Simao FA, Waterhouse RM, Ioannidis P, Kriventseva EV, Zdobnov EM. 2015. BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs. Bioinformatics 31: 3210-3212.
  9. Bruna T, Hoff KJ, Lomsadze A, Stanke M, Borodovsky M. 2021. BRAKER2: automatic eukaryotic genome annotation with Gene-Mark-EP+ and AUGUSTUS supported by a protein database. NAR. Genom. Bioinform. 3: lqaa108.
  10. Vaser R, Sovic I, Nagarajan N, Sikic M. 2017. Fast and accurate de novo genome assembly from long uncorrected reads. Genome Res. 27: 737-746.