• 제목/요약/키워드: Artificial propagation

검색결과 534건 처리시간 0.028초

Growth of Runner Plants Grown in a Plant Factory as Affected by Light Intensity and Container Volume

  • Park, Seon Woo;Kwack, Yurina;Chun, Changhoo
    • Horticultural Science & Technology
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    • 제35권4호
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    • pp.439-445
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    • 2017
  • Transplant production in a plant factory with artificial lighting provides several benefits; (1) rapid and uniform transplant production, (2) high production rate per unit area, and (3) production of disease free transplants production. To improve the growth of runner plants when strawberry transplants are produced in a plant factory, we conducted two experiments to investigate (1) the effect of different light intensity for stock and runner plants on the growth of runner plants, and (2) the effect of different container volume for runner plants on their growth. When the stock and runner plants were grown under nine different light conditions composed of three different light intensities (100, 200, and $400{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPF) for each stock and runner plants, increasing the light intensity for stock plants promoted the growth of runner plants, however, the growth of runner plants was not enhanced by increasing the light intensity for runner plants under same light intensity condition for stock plants. We also cultivated runner plants using plug trays with four different container volumes (21, 34, 73, and 150 mL) for 20 days after placing the stock plants, and found that using plug trays with lager container volume did not enhance the growth of runner plants. These results indicate that providing optimal condition for stock plants, rather than the runner plants, is more important for increasing the growth of the runner plants and that the efficiency of strawberry transplant production in a plant factory can be improved by decreasing light intensity or container volume for runner plants.

A Novel Hyperspectral Microscopic Imaging System for Evaluating Fresh Degree of Pork

  • Xu, Yi;Chen, Quansheng;Liu, Yan;Sun, Xin;Huang, Qiping;Ouyang, Qin;Zhao, Jiewen
    • Food Science of Animal Resources
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    • 제38권2호
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    • pp.362-375
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    • 2018
  • This study proposed a rapid microscopic examination method for pork freshness evaluation by using the self-assembled hyperspectral microscopic imaging (HMI) system with the help of feature extraction algorithm and pattern recognition methods. Pork samples were stored for different days ranging from 0 to 5 days and the freshness of samples was divided into three levels which were determined by total volatile basic nitrogen (TVB-N) content. Meanwhile, hyperspectral microscopic images of samples were acquired by HMI system and processed by the following steps for the further analysis. Firstly, characteristic hyperspectral microscopic images were extracted by using principal component analysis (PCA) and then texture features were selected based on the gray level co-occurrence matrix (GLCM). Next, features data were reduced dimensionality by fisher discriminant analysis (FDA) for further building classification model. Finally, compared with linear discriminant analysis (LDA) model and support vector machine (SVM) model, good back propagation artificial neural network (BP-ANN) model obtained the best freshness classification with a 100 % accuracy rating based on the extracted data. The results confirm that the fabricated HMI system combined with multivariate algorithms has ability to evaluate the fresh degree of pork accurately in the microscopic level, which plays an important role in animal food quality control.

Micropropagation of Aronia (Aronia melaocarpa Elliot, black chokeberry) and its 5 varieties (아로니아(Aronia melanocarpa Elliot) 5개 품종의 기내번식)

  • Kwak, Myoung-Chul;Choi, Chung-Ho;Choi, Yong-Eui;Moon, Heung-Kyu
    • Journal of Plant Biotechnology
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    • 제42권4호
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    • pp.380-387
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    • 2015
  • Aronia (Aronia melanocarpa, Black chokeberry) is an important cash crop in domestic agriculture. We investigated the effects of plant growth regulators on shoot proliferation and rooting using in vitro tissue culture. The most effective shoot multiplication was observed on WPM (woody plant medium) supplemented with 1.0 mg/L zeatin ($8.3{\pm}1.0$ shoots/explant), while the highest rooting rate was obtained from half-strength WPM with 3.0 mg/L IBA (8.8 roots/explant). The rooted plantlets all survived in the artificial soil mixture (with a mixture of peat moss : perlite : vermiculite, 1:1:1, v/v/v) and grew up relatively uniform, ranging from 14 to 16 leaves, 8 to 10 cm in stem height, and 2.3 to 2.8 mm in stem diameter. While experimenting with 5 different varieties of Aronia, we found out that each variety had different characteristics of shoot proliferation and rooting. The total numbers of proliferated shoots per variety is as follows: $17.4{\pm}0.8$ for Nero, 14 to 15 for Purple and Mackenzie, and 10 for both Viking and Odamamachiko. Rooting rates were also various depending on the variety: 88% of Odamamachiko, 80% of Viking and Purple, and 76% of Nero and 60% of Mackenzie shoots rooted. The survival rate of the rooted plantlets was from 92% to 100%, varying by type. Further growth appeared to be better in auxin-treated plantlets, compared to untreated ones. Our results showed the possibility of establishing an effective in vitro micropropagation system for Aronia melanocarpa.

Calibrating Stereoscopic 3D Position Measurement Systems Using Artificial Neural Nets (3차원 위치측정을 위한 스테레오 카메라 시스템의 인공 신경망을 이용한 보정)

  • Do, Yong-Tae;Lee, Dae-Sik;Yoo, Seog-Hwan
    • Journal of Sensor Science and Technology
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    • 제7권6호
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    • pp.418-425
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    • 1998
  • Stereo cameras are the most widely used sensing systems for automated machines including robots to interact with their three-dimensional(3D) working environments. The position of a target point in the 3D world coordinates can be measured by the use of stereo cameras and the camera calibration is an important preliminary step for the task. Existing camera calibration techniques can be classified into two large categories - linear and nonlinear techniques. While linear techniques are simple but somewhat inaccurate, the nonlinear ones require a modeling process to compensate for the lens distortion and a rather complicated procedure to solve the nonlinear equations. In this paper, a method employing a neural network for the calibration problem is described for tackling the problems arisen when existing techniques are applied and the results are reported. Particularly, it is shown experimentally that by utilizing the function approximation capability of multi-layer neural networks trained by the back-propagation(BP) algorithm to learn the error pattern of a linear technique, the measurement accuracy can be simply and efficiently increased.

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Front Classification using Back Propagation Algorithm (오류 역전파 알고리즘을 이용한 영문자의 폰트 분류 방법에 관한 연구)

  • Jung Minchul
    • Journal of Intelligence and Information Systems
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    • 제10권2호
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    • pp.65-77
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    • 2004
  • This paper presents a priori and the local font classification method. The font classification uses ascenders, descenders, and serifs extracted from a word image. The gradient features of those sub-images are extracted, and used as an input to a neural network classifier to produce font classification results. The font classification determines 2 font styles (upright or slant), 3 font groups (serif sans-serif or typewriter), and 7-font names (Postscript fonts such as Avant Garde, Helvetica, Bookman, New Century Schoolbook, Palatine, Times, and Courier). The proposed a priori and local font classification method allows an OCR system consisting of various font-specific character segmentation tools and various mono-font character recognizers. Experiments have shown font classification accuracies reach high performance levels of about 95.4 percent even with severely touching characters. The technique developed for tile selected 7 fonts in this paper can be applied to any other fonts.

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Neuro-Net Based Automatic Sorting And Grading of A Mushroom (Lentinus Edodes L)

  • Hwang, H.;Lee, C.H.;Han, J.H.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 한국농업기계학회 1993년도 Proceedings of International Conference for Agricultural Machinery and Process Engineering
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    • pp.1243-1253
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    • 1993
  • Visual features of a mushroom(Lentinus Edodes L) are critical in sorting and grading as most agricultural products are. Because of its complex and various visual features, grading and sorting of mushrooms have been done manually by the human expert. Though actions involved in human grading looks simple, a decision making undereath the simple action comes form the results of the complex neural processing of the visual image. And processing details involved in the visual recognition of the human brain has not been fully investigated yet. Recently, however, an artificial neural network has drawn a great attention because of its functional capability as a partial substitute of the human brain. Since most agricultural products are not uniquely defined in its physical properties and do not have a well defined job structure, a research of the neuro-net based human like information processing toward the agricultural product and processing are widely open and promising. In this pape , neuro-net based grading and sorting system was developed for a mushroom . A computer vision system was utilized for extracting and quantifying the qualitative visual features of sampled mushrooms. The extracted visual features and their corresponding grades were used as input/output pairs for training the neural network and the trained results of the network were presented . The computer vision system used is composed of the IBM PC compatible 386DX, ITEX PFG frame grabber, B/W CCD camera , VGA color graphic monitor , and image output RGB monitor.

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Turbidity Modeling for a Negative Buoyant Density Flow in a Reservoir with Consideration of Multiple Particle Sizes (입자크기 분포를 고려한 부력침강 저수지 밀도류의 탁도 모델링)

  • Chung, Se Woong;Lee, Heung Soo;Jung, Yong Rak
    • Journal of Korean Society on Water Environment
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    • 제24권3호
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    • pp.365-377
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    • 2008
  • Large artificial dam reservoirs and associated downstream ecosystems are under increased pressure from long-term negative impacts of turbid flood runoff. Despite various emerging issues of reservoir turbidity flow, turbidity modeling studies have been rare due to lack of experimental data that can support scientific interpretation. Modeling suspended sediment (SS) dynamics, and therefore turbidity ($C_T$), requires provision of constitutive relationships ($SS-C_T$) and accounting for deposition of different SS size fractions/types distribution in order to display this complicated dynamic behavior. This study explored the performance of a coupled two-dimensional (2D) hydrodynamic and particle dynamics model that simulates the fate and transport of a turbid density flow in a negatively buoyant density flow regime. Multiple groups of suspended sediment (SS), classified by the particle size and their site-specific $SS-C_T$ relationships, were used for the conversion between field measurements ($C_T$) and model state variables (SS). The 2D model showed, in overall, good performance in reproducing the reservoir thermal structure, flood propagation dynamics and the magnitude and distribution of turbidity in the stratified reservoir. Some significant errors were noticed in the transitional zone due to the inherent lateral averaging assumption of the 2D hydrodynamic model, and in the lacustrine zone possibly due to long-term decay of particulate organic matters induced during flood runoffs.

In vitro shoot initiation of Artocarpus heterophyllus Lam. (Jak Fruit) Effect of the explant type and the season of explant collection

  • Kahk, Kasturiarachchi;Wtpsk, Senarath;Lee, Kui-Jae
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 한국자원식물학회 2003년도 제10차 국제학술회의 및 추계정기 학술발표회
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    • pp.2-3
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    • 2003
  • A method for rapid propagation of mature Jack fruit was developed. Four types of explants (mature embryos, apical meristems of young seedlings, apices from mature plants and nodal segments) were used. It has been found 88% of young apical meristems produced shoots in Campbell and Durzan (CD) medium compared to 60% in Murashige and Skoog (MS) medium. Only 1/3 of them produced multiple shoots. Shoot initiation from nodal segments was very rare. Mature apices produced callus. Although removal of the sheathing cover around mature buds enhanced the shoot initiation but success rate was low in growth regulator free medium. Embryos respond to the CD medium but not to the MS medium. Embryos from seeds soaked in water for 24 hours produced shoots after 8 weeks of incubation and the success rate was 70% while embryos from dry seeds only produced roots. There was no significant effect of cold storage (refrigeration) for 7 days on shoot initiation from mature embryos (65%) but the ability for shoot induction declines with storage time (55% after 21 days of cold storage). Mature axillary buds were established in Modified Campbell and Durzan (CD) medium supplemented with 0.5mg/1 and IBA. There was a significant difference in the growth performance of shoots according to the period of the year in which explants were collected. Highest (60%) was observed in November-January period. It was only 30% when the explants were collected in February-April or May-July and decreased to 20% in August-October. The shoots produced in November-January showed a higher vigor than those produced in other months. Since Jak fruit show seasonal changes in fruit bearing and shedding of leaves, it can be suggested that the difference in growth performances of tissues cultured in artificial culture media would have been affected by endogenous rhythms.

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Characteristics of Multi-embryo Egg Capsule and Larvae of Mottled Skate Raja pulchra from Korea (한국산 참홍어(Raja pulchra)의 다배성 난각 특징과 자어의 형태)

  • Jo, Hyun-Su;Kang, Eon-Jong;Cho, Yeong-Rok;Seo, Hyung-Chul;Im, Yang-Jae;Hwang, Hak-Jin
    • Korean Journal of Ichthyology
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    • 제22권4호
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    • pp.217-224
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    • 2010
  • An investigation was carried out to obtain basic information needed to develop methods for artificial propagation and conservation of the mottled skate Raja pulchra, an important food resource in western Korea that has declined from overfishing. In this paper we provide evidence of multiple spawning and describe properties of the ovary, and morphology of the egg capsule and the fully-formed offspring. The vitellogenic follicles in the ovary was $179.8{\pm}57.1$ (54~247) and can be classified into five size groups, which the last group of ova are considered as the moving to the capsule gland where fertilization and encapsulation of ova take place. The morphology of the egg capsule of R. pulchra is unique among the species of the family Rajidae and showed multi-embryo characteristics, having two to six yolks in each capsule. The adaptive morphological changes of larvae developing inside the egg capsule are described based on specimens extracted from the capsule.

Effects of Manchurian Trout Gonadotropins on Sexual Maturation in Female Rainbow Trout

  • Park, Woo-Dong;Ko, Hye-Yeon;Kim, Dae-Jung;Lee, Cheul-Ho;Sohn, Young-Chang
    • Journal of Aquaculture
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    • 제20권3호
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    • pp.154-159
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    • 2007
  • Manchurian trout (Brachymystax lenok) is an endangered fish species in East Asia including the Korean peninsula. To establish a method for artificial propagation and to improve our understanding of the reproduction in the species, we have produced recombinant gonadotropins, follicle-stimulating hormone (r-mtFSH) and luteinizing hormone (r-mtLH), which may play central roles in reproductive activities. In the present study, the biological activities of the recombinant hormones were analyzed by gonadosomatic index (GSI), ovarian follicle diameter, and sex steroid levels in mature rainbow trout (Oncorhynchus mykiss). In the 6th day post-injection, FSH-injected fish were slightly decreased in the GSI value, although there were no significant differences among those of control, r-mtFSH, and r-mtLH treatments. Injection of the r-mtFSH increased follicle diameters significantly as compared with those of control- and r-mtLH-injected fish. The plasma steroid levels showed wide differences in the groups at 1, 3, or 6th day post-injection. Despite the variable steroid levels, three individuals receiving either r-mtFSH or r-mtLH showed a great increase in a maturation-inducing steroid, $17{\alpha},20{beta}$-dihydroxy-4-pregnen-3-one, at 3 and 6 days. Taken together, these results suggest that biological efficacies of the recombinant FSH and LH should be further studied in the Manchurian trout.