• BMB Reports
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• 제40권3호
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• pp.444-447
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• 2007

Polymerase chain reaction (PCR) is a powerful technique in molecular biology and is widely used in various fields. By amplifying DNA fragments, PCR has facilitated gene cloning procedures, as well as molecular genotyping. However, the extraction of DNA from samples often acts as a limiting step of these reactions. In particular, the extraction of PCR-compatible genomic DNA from higher plants requires complicated processes and tedious work because plant cells have rigid cell walls and contain various endogenous PCR inhibitors, including polyphenolic compounds. We recently developed a novel solution, referred to as AnyDirect, which can amplify target DNA fragments directly from whole blood without the need for DNA extraction. Here, we developed a simple lysis system that could produce an appropriate template for direct PCR with AnyDirect PCR buffer, making possible the direct amplification of DNA fragments from plant leaves. Thus, our experimental procedure provides a simple, convenient, non-hazardous, inexpensive, and rapid process for the amplification of DNA from plant tissue.

• Journal of Microbiology and Biotechnology
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• 제17권10호
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• pp.1616-1621
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• 2007

We have established a SYBR Green-based realtime PCR method using AnyDirect solution, which enhances PCR from whole blood, for direct amplification of the virA gene of Shigella flexneri and the invA gene of Salmonella typhimurium from human feces without prior DNA purification. When we compared the efficiency of conventional or realtime PCR amplification of the virA and invA genes from the supernatant of boiled feces supplemented with S. flexneri and S. typhimurium in the presence or absence of AnyDirect solution, amplification products were detected only in reactions to which AnyDirect solution had been added. The detection limit of real-time PCR was $1{\times}10^4\;CFU/g$ feces for S. flexneri and $2{\times}10^4\;CFU/g$ feces for S. typhimurium; this sensitivity level was comparable to other studies. Our real-time PCR assay with AnyDirect solution is simple, rapid, sensitive, and specific, and allows simultaneous detection of S. flexneri and S. typhimurium directly from fecal samples without prior DNA purification.

• 태양에너지
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• 제18권4호
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• pp.33-38
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• 1998

This paper reports the experimental results concerning the thermal performance of a freeze-protected direct solar water heating system. The direct system tested has been manufactured to prevent any breakage during harsh wintry conditions by specially designed riser and downcomer headers capable of relieving extra pressure due to freezing of water. The direct system tested has shown 10% improvement in its efficiency over that of a indirect type system using antifreeze as the heat transfer medium. It is felt that any direct system of its kind could galvanize the whole solar industry if further improvements are made to ensure its durability.

• 한국의류학회지
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• 제33권1호
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• pp.139-148
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• 2009

In this study, we tried to verify the effectiveness of 3D scans of clothed participants from 20 to 24 year old men in fit analysis process. Carrying out fit analysis, we used 3D scan data and direct fit evaluation with the basic garments made as semi-fitted bodice pattern for men. The result of fit evaluation through 3D scan data showed the expert rated fitting items more positive than direct fit evaluation. Even though, generally there was a no significant difference in the response between 3D and direct fit analysis. Only there was a significant difference in the response to the specific place such as the center front line, neckline, shoulder seam, and etc. There was a no significant difference between the result of 3D scan and direct fit evaluation in the subjects' group. Also the result of assessment of 3D targeting consumers showed very positive and interested in using 3D scans for fit analysis. The ability to rotate the 3D scans for a variety of views proved to be a very effective process to analyze fit. Moreover, digital data is easily accessed at any locations and any time. Fit analysis using 3D scans could be great tool for not only fit evaluation in research but also better fitting in apparel industry.

• 기본간호학회지
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• 제8권3호
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• pp.366-378
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• 2001

Objective: The purpose of this study was to identify differences in measurement methods for blood pressure (Direct measurement, HP automatic indirect measurement, and SE 7000 Korean made indirect measurement), and to evaluate the clinical trial of the Korean made automatic indirect blood pressure measurement. Material & Methods: From June, 1999 to February, 2000, fifty five consecutive patients were randomized into hypertension group (n=20), normotension group (n=20), and hypotension group (n= 15). Measuring blood pressure by indirect methods (HP NIBP M 1008B and SE 7000 NIBP Korean made) was done simultaneously in the same arm with arterial line for direct blood pressure measurement (HP M1006A). The procedures were repeated ten times at intervals of 2 minutes. Statistical analysis was Performed using SPSS (version 8.0 for windows) software package. Values were expressed as means and standard deviation and means were compared using t-test. Statistical significance was considered present with a p value less than 0.05. Results: In the hypertension group and noromotension group, the disparity between HP direct measurement and indirect SE 7000 NIBP did not show any differences compared to the disparity between HP direct measurement and indirect HP NIBP. In the hypotension group, the disparity in the diastolic pressure between HP direct measurement and indirect SE 7000 NIBP was significantly different compared to the disparity between HP direct measurement and indirect HP NIBP (p<0.001), however, disparities in systolic pressure did not showed any differences. Conclusion: Direct blood pressure measurement (HP M1006A) can be replaced with indirect blood pressure measurements (HP NIBP M 1008B & SE 7000 NIBP) in normotension and hypertension patients. Korean made indirect measurement was found to be more accurate compared to HP indirect measurement in hypotension Patients, but more study is needed.

• 대한전기학회논문지:전기기기및에너지변환시스템부문B
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• 제48권10호
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• pp.555-561
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• 1999

This paper proposes a new compensation algorithm for stator resistance that is crucial for improving the direct vector control performance of an induction motor. This algorithm is based on the flux estimator that is derived from the stator voltage equation. Since a flux estimator is dependent on the stator resistance, a flux error originates from the variation of the stator resistance. This parameter mismatch in the estimator thereafter affects the flux and torque response. Accordingly, a new compensator has been designed to offset this degradation in the responses. The proposed compensator is very simple to implement and does not require any modifications to the motor model or any special interruptions of the controller. The value of the stator resistance is attained in real time through measuring the terminal voltage and current. The effectiveness of the proposed scheme has been confirmed through both simulation and experimentation.

• 복식
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• 제32권
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• pp.57-68
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• 1997

The purpose of this study was to understand the effects of religion on Amish costumes. For this study I twice visited Amish village in Lancaster Pennsylvania U.S,A in July 1993 and August 1996. The methods of study directed direct observation and literature re-search. The results of this study were as follows: The Amish was livid practically by strict princi- ples of simplicity diligence piety and mutual cooperation. The Amish behavioral code of laws known as ordnung dictated appropriate dress agricultual methods and a routine for daily life. The Amish costumes originated with the 17th century Puritans traditional apparel the European farmer and the clothing style of 19th century Americal,. Amish costumes was fastened with hooks and eyes instead of decorative buttons a diret influence of Puritanism does and did non con-tain ornamental outer pockets. This distinctively simple costumes served as a boundary outsiders to and as a direct ex-pression of their faith. An Amish doll had neither facial features nor fingers and toes as described in scripture "You do not become corrupt and make for yourselves an idol an image of any shape whether formed like a man or a women or like any animal on earth or any bird that flies in the air or like any creature that moves along the ground or any fish in the waters below" In this way Amish costumes was affected by their religion and faith.

• 한국태양에너지학회 논문집
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• 제26권3호
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• pp.53-62
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• 2006

Since the direct normal radiation and clear day are a main factor for designing any solar thermal and photovoltaic concentrating system, it is necessary to evaluate its characteristics all over the country. The work presented here are the investigation of direct normal radiation and clear day in Korea. The data utilized in the investigation consist of direct normal radiation collected for 14 years('90. 12${\sim}$2004. 12) and clear-day collected for 23 years($1982{\sim}2004$) at measuring stations across the country. The analysis shows that the annual-average daily direct normal radiation is 5.4 kWh/m2 and the annual-average total clear-day is 92.7 days in Korea. We also constructed the contour map of direct normal radiation and clear-day in Korea by interpolating actually measured data across the country.

• 한국농공학회지
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• 제36권1호
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• pp.54-62
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• 1994

The expanding technique of the Stochastic Finite Element Method(SFEM) is proposed in this paper for adapting direct integration methods in stochastical dynamic analysis of structures. Grafting the direct integration methods and the SFEM together, one can deal with nonlinear structures and nonstationary process problems without any restriction. The stochastical central diffrence and stochastic Houbolt methods are introduced to show the expanding technique, and their adaptabilities are discussed. Results computed by the proposed method (the Stochastic Finite Element Method in Dynamics: SFEMD) for two degree-of-free- dom system are compared with those obtained by Monte Carlo Simulation.

• 대한기계학회논문집A
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• 제21권11호
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• pp.1819-1826
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• 1997

Direct-drive robots are suitable to the position and force control with high accuracy, but it is difficult to design a controller because of the system's nonlinearity and link-interactions. This paper is concerned with the study of the force control of direct-drive robots. The proposed algorithm consists of feedback controllers and a neural network. After the completion of learning, the output of feedback controller is nearly equal to zero, and the neural network controller plays an important role in the control system. Therefore, the optimum retuning of parameters of feedback controllers is unnecessary. In other words, the proposed algorithm does not require any knowledge of the controlled system in advance. The effectiveness of the proposed algorithm is demonstrated by the experiment on the force control of the parallelogram link-type direct-drive robot.