• Title/Summary/Keyword: Antioxdative activity

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Antioxidative and Nitrite-scavenging Effects of Solvent Extracts from Gyrophora esculenta (석이버섯 용매 추출물의 항산화 및 아질산염 소거작용)

  • 정은재
    • The Korean Journal of Food And Nutrition
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    • v.11 no.4
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    • pp.426-430
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    • 1998
  • This study was conducted to investigate electron donating ability, antioxdative activity and nitrite-scavenging effect of solvent extracts from Gyrophora esculenta. The phenolic compounds of diethylether, butanol, petroleum ether and hexane extracts were 194.20$\mu\textrm{g}$/ml, 98.80$\mu\textrm{g}$/ml, 1.60$\mu\textrm{g}$/ml and 0.20$\mu\textrm{g}$/ml, respectively. Electron donating abilities of diethyleter and butanol extracts from Gyrophora esculaenta were 80.79% and 72.81%, respectively. Absorption of DPPH by diethyleter and butanol extracts sharply increaed at initial stage. Antioxidative activities of fiethylether and butanol extracts by peroxide values are rather high as compared to those of control, but low s compared to those of BHA. Nitrite-scavenging abilities of diethylether, butanol and petroleum ether from Gyrophora esculenta were 96.07%, 77.39% and 15.07%, respectively.

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Antimicrobial and Antioxidative Effects of Alismatis Rhizoma Extract (택사 추출물의 항균 및 항산화 효과)

  • 서권일;조영숙;박정로;이성태;박채규
    • Journal of Life Science
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    • v.10 no.5
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    • pp.524-528
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    • 2000
  • Antimicrobial and antioxidative effects of Alismatis Rhizoma which has been used as a galenic for diuretic and antiphlogistic were investigated. In a paper disc test, water extract of Alismatis Rhizoma showed antimicrobial activities against almost all of the microbial species tested, while their activities of methanol and ethanol extract was low than the activity of water extract, and the antimicrobial activity of water extract was most prominent against Vibrio parahaemolyticus. Antioxidative activity against linoleic acid was not observed in methanol extract. In contrast to methanol and ethnol and ethanol extract, water extract showed a antioxidative activity against linoleic acid. However, the antioxidative of water extract was lower than that of 0.1% BHT.

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Antioxdative Effect of the Essential Oil form the Rhizomes of Acorus gramineus (석창포 정유성분의 항산화활성)

  • 구병수;이동웅
    • Journal of Life Science
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    • v.11 no.6
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    • pp.503-508
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    • 2001
  • The essential oil of Acorus gramineus(Aracease), which has been used as an antioconvulsant in Korean folk medicine, was evaluated for its effects on antioxidative system in vitro and in vivo. This mixture of terpenes showed inhibitory effects on xanthine oxidase activity with 13.3% at 10 $\mu\textrm{g}$/ml and on aldehyde oxidase activity with 5.0% at 1$\mu\textrm{g}$/ml. Lipid perosidation was inhibited by 49.4% at 1.0 mg/ml of the essential oil in vitro and by 16.7% after 7 days inhablation of an oil as compared to PTZ-treated control group. DPPH radical scavenging activity of this essential oil was ralatively werak.

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The Quality Characteristics and Antioxidative Activity of Sulgidduk Supplemented with Ramie Leaf Powder (모시풀잎 분말 첨가 설기떡의 품질특성 및 항산화 활성)

  • Park, Seong-Soon;Kim, Sun-Im;Sim, Ki-Hyeon
    • Korean journal of food and cookery science
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    • v.27 no.6
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    • pp.763-772
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    • 2011
  • This study evaluated the antioxidative activity and quality characteristics of Sulgidduk (traditional rice cakes) prepared with ramie leaf powder, in order to measure the antioxidative activity of ramie leaves, which are a potentially useful antioxidant food. Antioxidant activities were measured by total phenolic contents, the scavenging activities of DPPH radicals and ABTS+ radicals, and the reducing power. The total phenolic content of freeze dried ramie leaves was estimated at $58.70{\pm}11.42mg$ GAE/g. The $IC_{50}$ values for DPPH radicals and the ABTS+ radical scavenging activity of the ramie leaves were $23.76{\pm}1.41ug/mL$ and $174.82{\pm}6.4ug/mL$, respectively. The leaves also showed a high reducing power of $1.1057{\pm}0.03$ (O.D). To increase the utilizability of ramie leaf, we made Sulgidduk with added ramie leaf powder. Re-grading the quality characteristics of the altered Sulgidduk, the 3% and 5% addition levels most satisfied texture profile analysis and sensory evaluation. The antioxidative effect of the Sulgidduk significantly increased as the addition level increased, compared to the original Sulgidduk. In term of DPPH radical scavenging activity, the 5% or more addition level Sulgidduk showed an antioxdative effect increase of more than 50%. At the 3% or higher addition level, there was higher antioxidant activity than in the positive control group, which included vitamin C and BHT additions. We thus concluded that Sulgidduk with added ramie leaf power can be manufactured as a food that can satisfy sensory preferences as well as boosting antioxidant activity, at both the 3% and 5% addition level.

Antioxidative Materials in Domestic Meju and Doenjang 2. Separation of Lipophilic Brown Pigment and Their Antioxdative Activity (재래식 메주 및 된장중의 항산화성 물질에 관한 연구 2. 지용성 갈변물질의 분리와 항산화력)

  • Kim, Mi-Hye;Im, Sang-Sun;Kim, Sung-Hee;Kim, Gyeong-Eup;Lee, Jong-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.2
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    • pp.251-260
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    • 1994
  • Lipophilic brown pigments produced during the fermentation of domestic Meju and Doenjang were fractionated by column and thin -layer chromatography (TLC). Each of the fractions was tested for the antioxidant activity and then characterized by spectroscopic analysis. The lipophilic brown pigments were separated into chloroform -soluble and methanol-soluble parts in which Meju resulted the higher content of chloroform-soluble part than that of methanol-soluble part ; however, Doenjang exhibited the opposite result to that of Meju. More strong antioxidant activity was found in the methanol-soluble part than the chloroform-soluble part. Four and five fractions were separated from chloroform-soluble and methanol-soluble parts respectively. by silicagel TLC. The fraction that exhibited the high antioxidant activity showed a strong absorption at 260nm caused by amino compounds in UV spectrum The other fractions which did not have antioxidant activity absorbed at 240nm by carbonic acid and it ester,. IR spectrum of each fraction commonly showed absorption at 3400cm-1 , 2800cm-1 , 1700cm-1, 1600cm-1, 1400cm-1 , 1300cm-1 and 1100cm-1. Especially , the fraction which had a strong antioxidant activity showed absorption at 2800cm-1, 1400cm-1, 1600cm-1 suggesting that the fraction contain Schiff's base and primary amine structure.

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Studies on Biological Activity of Wood Extractives (XI) - Compounds from Heartwood of Taxus cuspidata and Their Antioxdative Activities - (수목추출물의 생리활성에 관한 연구(XI) - 주목(Taxus cuspidata) 심재 추출성분 및 항산화활성 -)

  • Lee, Hak-Ju;Lee, Sung-Suk;Choi, Don-Ha;Kwon, Yeong-Han
    • Journal of the Korean Wood Science and Technology
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    • v.31 no.1
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    • pp.32-40
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    • 2003
  • Antimicrobial and antioxidative activities of heartwood extractives of domestic species were investigated to develop a natural fungicide or preservative. Four lignan derivatives and one taxane were isolated from heartwood of Taxus cuspidata which has been selected due to its high antioxidative activity among the tested species. The chemical structures were identified as : taxusin, isolariciresinol (4, 4', 9, 9'-tetrahydroxy-3', 5-dimethoxy-2, 7'-cyclolignan), lariciresinol (4, 4', 9-trihydroxy-3, 3'-dimethoxy-7, 9'-epoxylignan), taxiresinol (3, 4, 4', 9-tetrahydroxy-3'-methoxy-7, 9'-epoxylignan) and isotaxiresinol (3', 4, 4', 9, 9'-pentahydroxy- 5-methoxy-2, 7'-cyclolignan) on the basis of spectroscopic data and their chemical correlations. According to the results of free radical scavenging activity, isolariciresinol, lariciresinol and isotaxiresinol showed higher radical scavenging activity than those of 𝛼-tocopherol and butylated hydroxytoluene (BHT), the strongest natural and synthetic antioxidants. However, taxusin did not show any free radical scavenging activity. In this regard, it could inferred that high antioxidative activity of extractives of T. cuspidata was derived from isolariciresinol, lariciresinol and isotaxiresinol.

Anitoxidative components of pueraria root (칡뿌리의 항산화 성분)

  • Oh, Man Jin;Lee, Ka Soon;Son, Hwa Young;Kim, Seung Yeol
    • Korean Journal of Agricultural Science
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    • v.17 no.1
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    • pp.52-64
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    • 1990
  • As a search for natural antioxidants, antioxdative fractions in Pueraria roots were extracted, identified using column chromatography, thin layer chromatography or high performance liquid chromatography. The components which have most effective antioxidative activities were further identified by IR and GC-MS. Separated antioxidative components were then added to four different oils to examine their antioxidative activities. Yield of extract obtained from pueraria root powder by solvent extraction using four step solvent systems was 2.54%. Antioxidative activity of the extracts was as effective as that of 100 ppm ${\delta}$-tocopherol addition, when 0.1% of the extracts was added to linoleic acid. The strongest antioxidative component of methanol extract of pueraria root was identified as puerarin. Aunioxidative activity of puerarin on lard was more effective than ${\alpha}$-tocopherol, but less effective than ${\delta}$-tocopherol. When the puerarin was added to edible oil and heat treated at $145^{\circ}C$, the acid value was lowest in lard and was highest in soybean oil. Antioxidative activity in terms of carbonyl value, thiobarbituric acid value and anisidine value was most high in palm oil and least in soybean and cottonseed oil.

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Total Phenol Contents and DPPH Radical Scavenging Activity of Entomopathogenic Fungi (곤충병원성진균의 총 페놀 함량 및 DPPH 라디칼 소거능)

  • Lee, Ki-Man;Nam, Sung-Hee;Song, Ha-Suk;Yeo, Joo-Hong;Lee, Kwang-Gill;Bae, Yoon-Hwan
    • Korean journal of applied entomology
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    • v.48 no.3
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    • pp.377-383
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    • 2009
  • This study was conducted to investigate the total phenol contents, antioxidative activities and antibacterial activities of twenty species of entomopathogenic fungi. The total phenol content was highest in Aspergillus flavus ($553.0{\pm}52.15{\mu}g/g$) and A. parasiticus ($529.9{\pm}60.10{\mu}g/g$). On the other hand those in other strains were within the range of $26.6{\sim}121.9{\mu}g/g$. The antioxdative activity was shown in the most of strains and the highest DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging activity was observed in A. flavus ($90.9{\pm}2.90%$) and A. parasiticus ($77.9{\pm}4.13%$). This result indicated that the antioxidative activities were very correlated with the total phenol contents. The antibacterial activitiy was found in the every tested pathogenic bacteria. Especially, the antibacterial activity was strongest against Listeria monocytogenes and Escherchia coli.

Anti-obesity Effects of Mulberry Leaf and Yacon Extract in Obese Rats

  • Lim, Yong;Oh, Ji Hye;Park, Un Kyu;Huh, Man Kyu;Hwang, Seock-Yeon
    • Biomedical Science Letters
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    • v.26 no.2
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    • pp.75-84
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    • 2020
  • We evaluated the effect of anti-obesitic activity of MYE (mulberry leaf + Yacon tuber) extracted from Morus alba as muberry leaf and Smallanthus sonchifolia as yacon. 1%, 3%, or 5% of MYE were treated to Sprague-Dawley rats exposed to a high-fat diet. MYE treated rats were suppressed weight during four weeks, and they lost weight significantly after six weeks. Common blood chemistry panels related to liver function revealed significant improvement in the MYE-treated groups. The expression of leptin as indicators for obesity was decreased in perirenal fat. Such results indicate that MYE could be a promising candidate for the improvement of obesity. In addition, MYE effected on deceased glucose metabolism, reducing the activities of glucose-6-phosphate dehydrogenase (G-6-PDH) and glucokinase related to glycogen synthesis. The fatty liver was observed in high-fat diet-treated rats, resulting from increased number of adipose cells and Ito cells. However, this pathologic change was significantly improved by administration of MYE. MYE have significant effects on antioxdative function and glycometabolism against high fat diet. Thereby, it seems that MYE prevent fatty liver by high-fat diet. Thus it is suggested that MYE would be worth being developed as an biofunctional food to prevent undesirable effects caused by obesity.

Characteristics of Barley Polyphenol Extract (BPE) Separated from Pearling By-products (보리 도정부산물로부터 분리한 폴리페놀 추출물의 특성)

  • Seog, Ho-Moon;Seo, Mi-Sook;Kim, Sung-Ran;Park, Yong-Kon;Lee, Young-Tack
    • Korean Journal of Food Science and Technology
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    • v.34 no.5
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    • pp.775-779
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    • 2002
  • Barley brans, divided into fractions I $(pearlers\;1{\sim}4)$, II $(pearlers\;5{\sim}20)$, and III $(pearlers \;21{\sim}24)$, germ, and broken kernels were collected as pearling by-products produced by an industrial process consisting of 24 consecutive barley pearlers. The pearling by-products were extracted with 75% ethanol, and polyphenol extracts were separated using Sepabeads SP-850 resin. Total polyphenol content was the highest (43.68%) in the polyphenol extract separated from fraction I. Polyphenol compounds analyzed by HPLC were tentatively identified as proanthocyanins and prodelphinidin polymers, which were known to have antioxidative and antimutagenic activities. Antioxidative abilities were estimated to be in the following order: fraction I>germ>fraction II>fraction III. At 0.01% polyphenol extract, angiotensin I-converting enzyme inhibitory activity was the highest in the germ fraction (40.04%). Nitrite-scavenging activity was over 70% in all fractions. Superoxide dismutase-like activity was in the range of $64{\sim}78%$ in fractions I and II.