• 한국미생물·생명공학회지
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• 제37권4호
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• pp.340-349
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• 2009

메주로부터 항진균 및 항세균 활성을 나타내는 균주를 분리하고 동정하여 B. polyfermenticus CJ9로 명명하였다. B. polyfermenticus CJ9의 생육에 따른 항균 활성을 측정한 결과 항세균 활성은 배양 12시간에 최대 활성을 나타내며 72시간까지 90% 이상 활성을 유지하다 120시간에 활성을 완전히 상실하였다. 항진균 활성은 배양 24시간 이후부터 최대 활성을 나타내었고, 사멸기 이후 활성이 다소 감소되었으나 배양 120시간까지 활성이 유지되었다. B. polyfermenticus CJ9은 식품과 인체에 유해한 곰팡이, 효모, 그람 양성 및 음성 세균에 대한 항진균 활성과 항세균 활성을 동시에 나타내었다. B. polyfermenticus CJ9의 항세균 활성은 $37^{\circ}C$ 에서 24시간 열처리 후에 활성을 상실하였으며, pH 5.0~9.0 구간에서는 안정한 활성을 나타내었으나 pH 3.0~4.0 구간에서 활성이 감소하였다. 항진균 물질은 $121^{\circ}C$에서 15분간 열처리시 활성이 감소되었으나 역가가 완전히 소실되지 않았으며, pH 3.0~9.0 구간에서 안정한 활성을 나타내었다. 항세균 물질과 항진균 물질은 proteinase K, protease, trypsin, $\alpha$-chymotrypsin 등의 단백분해효소 처리로 역가를 상실하거나 일부 감소되어 단백질성 물질임을 추정하였다. B. polyfermenticus CJ9의 항세균 물질과 항진균 물질을 $C_{18}$ Sep-Pak column에 흡착된 분획으로부터 역가를 확인하여 소수성 물질임을 알 수 있었으며, Tricine-SDS-PAGE 및 direct detection 실험을 통하여 분자량을 확인한 결과 항진균 물질은 약 1.4 kDa의 물질임을 확인하였다. 그러나 항세균 활성 물질은 열 불안정성 때문에 동 실험법상에서 그 분자량을 확인할 수 없었다. B. polyfermenticus CJ9이 생산하는 항균 물질은 항세균 및 항진균 활성을 동시에 가지는 단백질성 물질로서 천연 식품보존제 및 정장제재로 활용이 기대되며, 이를 위하여 항세균 물질과 항진균 물질의 정제 및 구조분석 등의 연구가 필요하다.

• Natural Product Sciences
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• 제9권3호
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• pp.154-157
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• 2003

In the present study, the roots of Sesbania grandiflora L. Poiret (Papilionaceae) were successively extracted with petroleum ether (PE), chloroform (CE), methanol (ME) and water (AE) by soxhlet extraction. The extracts were vacuum dried and screened for analgesic, antidiarrhoeal, antibacterial (Staphylococcus epidermidis, Staphylococcus aureus, Micrococcus luteus, Bacillus cereus, and Klebsiella pneumonia) and antifungal (Candida albicans and Aspergillus niger) activity. All the extracts exhibited potent, dose dependant (40 and 80 mg/kg) and significant analgesic and antidiarrhoeal activity in the order of AE>PE>CE>ME and ME>PE>AE>CE respectively. AE at the experimental dose was found to exhibit more potent analgesic activity than standard drug. All the extracts exhibited significant antibacterial $(100\;{\mu}g/ml)$ and antifungal activity $(50\;and\;100\;{\mu}g/ml)$. ME exhibited the most potent antibacterial activity.

• Archives of Pharmacal Research
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• 제29권7호
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• pp.535-540
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• 2006

Several novel 1-[2-(1H-tetrazol-5-yl) ethyl]-1H-benzo[d][1,2,3]triazoles (3a-h) have been synthesized by the condensation of 1-[2-(1H-tetrazol-5-yl)-ethyl]-1H-benzotriazole (2) and appropriate acid chlorides. 1-[2-(1H-tetrazol-5-yl)-ethyl]-1H-benzotriazole (2) was synthesized by reacting 3-(1H-benzo[d][1,2,3]triazol-1-yl)propanenitrile with sodium azide and ammonium chloride in the presence of dimethylformamide. The synthesized compounds were characterized by IR and PMR analysis. The titled compounds were evaluated for their in vitro antibacterial and antifungal activity by the cup plate method and anticonvulsant activity evaluated by the maximal electroshock induced convulsion method in mice. All synthesized compounds exhibited moderate antibacterial activity against Bacillus subtilis and moderate antifungal activity against Candida albicans. Compounds 5-(2-(1 H-benzo[d][1,2,3]triazo-1-yl)ethyl)-1H-tetrazol-1-yl)(4-aminophenyl)methanone 3d and 5-(2-(1 H-benzo[d][1,2,3]triazo-1-yl)ethyl)-1H-tetrazol-1-yl)(2-aminophenyl)methanone 3e elicited excellent anticonvulsant activity.

• 패션비즈니스
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• 제14권1호
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• pp.176-183
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• 2010

The purpose of this study is to examine the antibacterial activity and deodorization of fabrics dyed with pine needles extract. The microbes used for a test were two kinds of bacteria and two kinds of fungi. The test results showed as follows. In the test results of antimicrobial activity, both cotton and silk fabrics showed the antimicrobial rate of over 90% after washing three times. In the test result of antifungal activity, all fabrics didn't show any effective antifungal activity. In the test result of deodorization, the dyed cotton fabric showed the higher rate of deodorization than the non-dyed fabric, and the fabric showed the highest rate of deodorization was the test fabric mordanted by Fe and the rate of deodorization showed 92%. In case of silk fabric, the fabric showed the highest rate of deodorization was the test fabric mordanted by Al, and the rate of deodorization showed 92%.

• 약학회지
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• 제36권2호
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• pp.110-114
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• 1992

In order to evaluate the antimicrobial effect of 2, 3-substituted-1, 4-naphthoquinone derivatives, we newly synthesized several 2-chloro, 2-bromo and 2-hydroxy-1, 4-naphthoquinones and subjected to antibacterial and antifungal activities, in vitro, against Escherichia coli NIHJ, Staphylococcus aureus ATCC6538p, Candida albicans 10231, Aspergillus niger 1231 and Tricophyton mentagrophytes 6085. Among these derivatives 3, 9, 18 and 23 showed the potent antibacterial activities. 18, 23 and 28 have the antifungal activities. However, these compounds have no significant hemolytic activity at concentrations higher than that required for showing the antibacterial and antifungal activities.

• 현장농수산연구지
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• 제21권1호
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• pp.71-80
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• 2019

우리나라 자원식물로 오랫동안 한약재로 이용하고 있는 청미래덩굴, 약모밀, 삼백초, 줄풀 총 4종의 야생화를 산업화하기 위해 실험을 하였다. 청미래덩굴, 약모밀, 삼백초는 뿌리를 사용하였고 줄풀은 잎을 사용하였다. 추출은 50% ethyl alcohol에서 하였고, 이를 농축하여 재료를 냉동보관 한 후 100 ppm의 저농도를 투입했다. 이상의 방법으로 4종의 야생화를 이용한 항균력과 항진균력을 살펴본 결과는 다음과 같다. 1. 청미래덩굴, 삼백초, 약모밀, 줄풀을 알코올로 추출 후 분말화하여 살균과 진균 test를 한 결과 Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa에 대한 억제력은 약모밀에서 가장 높게 나타났고 줄풀은 억제력은 어느 정도 입증되었다. 2. 진균 종류인 Candida albicans, Aspergillus niger, Aspergillus fumigatus, Cryptococcus neoformans에 대한 항진균 실험에서는 Candida albicans에 대한 억제력은 미미하였지만 일부 확인하였으며 다른 진균에 대한 억제력은 전혀 나타나지 않았다.

• 대한화학회지
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• 제58권4호
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• pp.366-376
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• 2014

Synthesis, structural characterization, and biological activity studies of novel pyrido[2,3-d]pyrimidines (10a-h, 11a-h) are described. Cyclization of cynoacetamides (4, 5) with malonitrile (7) and aldehyde (6a-h) via Hantzsch pyridine synthesis afforded cyanopyridones (8a-h, 9a-h), which on cyclization with formic acid under microwave conditions led to the final product. All the reactions are significantly faster and the isolated yields are remarkably higher in microwave conditions compared to the conventionally heated reactions. The compounds were tested in vitro for their antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtillus, Staphylococcus aureus, and Micrococcus luteus and antifungal activity against Trichphyton longifusus, Candida albicans, Microsporum canis, Fusarium solani. Compounds 10b, 10e, 11b and 11e exhibited good antibacterial and antifungal activities compared with standards.

• 셀메드
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• 제2권4호
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• pp.37.1-37.4
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• 2012

In the present study, the volatile oil, chloroform extract and methanol extract of the woods of the plants Cedrus deodara and Pinus roxburghii were screened for their antibacterial and antifungal activities against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Streptococcus pyogenes, Candida albicans, Aspergillus niger, and Aspergillus clavatus using the Agar diffusion method. The susceptibilities of the microorganisms to the extracts were compared with each other and with a selected standard antibiotic. It was observed that the volatile oil and chloroform extracts showed the significant antibacterial activities while the least antibacterial activity was recorded with the methanolic extracts. The higher Minimum Inhibitory Concentration value of the extracts and oils against fungus suggested that the plants may possess less antifungal activity. Phytochemical analysis and thin-layer chromatography profiling revealed the presence of flavonoids and terpenoids in the oil and chloroform extracts, which could explain the antimicrobial activity. The findings suggest that the Cedrus deodara and Pinus roxburghii have antimicrobial properties and they can be used in the treatment of infectious diseases. However, further work is required in order to isolate the active constituents of the plants responsible for the antibacterial activity.

• KCI Concrete Journal
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• 제14권4호
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• pp.171-177
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• 2002

Antifungal agents are used to impart antibacterial or bactericidal properties to commodities and various articles used in industries and can be classified into two broad groups i.e organic and inorganic. Inorganic antifungal agents comprise of Ag, Zn, or Cu, etc. These elements tend to exhibit high level of antifungal activities, non-uniform dispersion in substrates, and have poor properties in expensive and cheap adhesiveness. In this study, the organic antifungal agent was used for the purpose of investigating the antifungal activity of antifungal agent activated-cement mortar (AACM) on the aspergilus niger of various fungus which can be easily discovered in the interiors and exteriors of buildings. In addition, an experiment on the basic physical properties of AACM such as compressive and flexural strength was carried out. The conclusion of this investigation revealed that a dosage increase of antifungal agent exhibits a high inhibitory effect on the aspergilus niger, and although there is a slight decrease in the strength of AACM, the strength of AACM was almost equal to that of inactivated cement mortar.

• Archives of Pharmacal Research
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• 제13권1호
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• pp.14-18
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• 1990

The carbinols, hydrazide, Schiff's bases and acryloyl derivatives of 2-thiobenzethiazole were prepared. The acid and nitrile of 20thiobenzothiazole were also obtained. The derivatives were tested for their antibacterial activities. Some derivatives showed the highest antifungal and activity.