Journal of Practical Agriculture & Fisheries Research (현장농수산연구지)

Korea National University of Agriculture and Fisheries (국립한국농수산대학교 교육개발센터)
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Development of Antibacterial Using Several Wild Flowers

야생화를 이용한 살균제 개발

  • Jung, J.H. (BiBi FLORA Natural Research and Development) ;
  • Park, N.B. (Department of Floriculture, Korea National College of Agriculture and Fisheries)
  • 정재한 (비비플로라 천연물연구소) ;
  • 박노복 (국립한국농수산대학 화훼학과)
  • Received : 2019.03.29
  • Accepted : 2019.05.26
  • Published : 2019.06.28
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Abstract

We have been experimenting for industrialize four kinds of wildflowers(Smilax china, Saururus chinensis, Houttuynia cordata and Zizania caduciflora) which are used for Korean medicinal plants for a long time as Korean resources plants. Smilax china, Saururus chinensis, Houttuynia cordata was used for roots and Zizania caduciflora used for leaves. Extraction was carried out in 50% ethyl alcohol, which was concentrated by freezing the material and then injected at a low concentration of 100ppm. The results of the study on the antibacterial potential activity and antifungal potential activity using the four wildflowers strains were as follows. 1. The results of the sterilization and antibacterial potential activity and antifungal potential activity were as follows. Houttuynia cordata showed good inhibitory effect for Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. The inhibitory power of Zizania caduciflora was proved to some extent. However, other cultivars showed little antibacterial and antifungal activity. 2. The antifungal potential activity against Candida albicans, Aspergillus niger, Aspergillus fumigatus, and Cryptococcus neoformans was small but some inhibition against Candida albicans was confirmed. There was no inhibition of other fungi potential at all.

우리나라 자원식물로 오랫동안 한약재로 이용하고 있는 청미래덩굴, 약모밀, 삼백초, 줄풀 총 4종의 야생화를 산업화하기 위해 실험을 하였다. 청미래덩굴, 약모밀, 삼백초는 뿌리를 사용하였고 줄풀은 잎을 사용하였다. 추출은 50% ethyl alcohol에서 하였고, 이를 농축하여 재료를 냉동보관 한 후 100 ppm의 저농도를 투입했다. 이상의 방법으로 4종의 야생화를 이용한 항균력과 항진균력을 살펴본 결과는 다음과 같다. 1. 청미래덩굴, 삼백초, 약모밀, 줄풀을 알코올로 추출 후 분말화하여 살균과 진균 test를 한 결과 Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa에 대한 억제력은 약모밀에서 가장 높게 나타났고 줄풀은 억제력은 어느 정도 입증되었다. 2. 진균 종류인 Candida albicans, Aspergillus niger, Aspergillus fumigatus, Cryptococcus neoformans에 대한 항진균 실험에서는 Candida albicans에 대한 억제력은 미미하였지만 일부 확인하였으며 다른 진균에 대한 억제력은 전혀 나타나지 않았다.

Keywords

References

  1. 권설안. (1999). 속 삼백초와 어성초에 대하여. 대한한약 2 : 18-20
  2. 박종희, 이정규. (2000). 상용약용식물도감. 도서출판 신일상사, 서울. 202-203
  3. 정연옥, 박노복, 곽준수, 정숙진. (2010). 야생화 백과사전(봄편). 푸른행복. 718-721
  4. Ahn, S. M., T. H. Choi, I. S. Kwun, and H. Y. Sohn. (2011). Antifungal Activity of Methylene Chloride Fraction of Pimpinella brachycarpa Against Aspergillus niger, Korean J. Microbiol. Biotechnol. 39(2) : 168-74
  5. Bernstein EF, Chen YQ, Tamai K, Shepley KJ, Resnik KS, Zhang H, Tuan R, Mauviel A, Uitto J. (1994). Enhanced elastin and fibrillin gene expression in chronically photodamaged skin. J Invest Dermatol 103 : 1802-186
  6. Blanken R, Van Vilsteren MJT, Tupker RA, Coenraads PJ. (1989) Effect of min eral oil and linoleic-acid-containing emulsions on the skin vapour loss of sodium-laurylsulphate-induced irritant skin reactions. Contact Dermatitis 20(2) : 93-97
  7. Bown D. (1995). Royal Horticultural Society Encyclopedia of Herbs and Their Uses, p. 424, Dorling Kindersley, London, UK.
  8. Elias PM. (1983). Epidermal lipids, barrier function, and desquamation. J Invest Dermatol 80 : 44-49
  9. Elias PM, Menon GK. (1991). Structural and lipid biochemical correlates of the epidermal permeability barrier. Adv Lipid Res 24 : 1-26
  10. Eum J. S. (2012). Activity of Nature Plants Extracts against Pseudomonas aeruginosa ATCC 27853, 한국정보통신학회논문지 16(8) : 1799-1804
  11. Gilchrest BA. (1989). Skin aging and photoaging: an overview. J Am Acad Dermatol 21 : 610-613
  12. Jeong H. R., J. H. Kwak, J. H. Kim, G. N. Choi, C. H. Jeong, and H. J. Heo. (2010). Antioxidant and neuronal cell protective effects of an extract of Houttuynia cordata Thunb (a culinary herb), Korean J. Food Preserv., 17(5) : 720-726
  13. Kim G. S., D. H. Kim, J. J. Lim, J. J. Lee, D. Y. Han, W. M. Lee, W. C. Jung, W. G. Min, C. G. Won, M. H. Rhee, H. J. Lee, and S. Kim. (2008), Biological and antibacterial activities of the natural herb Houttuynia cordata water extract against the Intracellular bacterial pathogen Salmonella within the RAW 264.7 macrophage, Biol. Pharm. Bull, 31(11) : 2012-2017
  14. Kim J., H. S. Ryu, J. H. Shin, and H. S. Kim. (2005). In vitro and ex vivo supplementation of Houttuynia cordata extract and im-munomodulating effect in mice, J. Korean Soc. Food Sci. Nutr., 34 : 167-175
  15. Kim K. Y., D. O. Chung, and H. J. Chung. (1997). Chemical composition and antimicrobial activities of Houttuynia cordata Thumb., Korean J. Food Sci. Technol., 29(3) : 400-406
  16. Kwak J. W, C. H. Kwon. (1988). Pharmacological studies on Saururus chinensis baill. Bull KH Pharma Sci 16 : 137-154
  17. Lee J. H., Y. M. Lee and Y. M. Han. (2011). Antifungal Activity of Glycycoumarin to Candida albicans, Yakhak Hoeji, 55(3) : 234-239
  18. Lee, E. S., B. I. Seo. (2001). Portulaca oleracea L. 추출물에 의한 Escherichia coli KCTC 2441의 생육억재, Herbal formula science, 9(1) : 367-374
  19. Lee J. T., Y. S Jeong, B. J. An. (2002). Physiological activity of Salicornia herbacea and its application for cosmetic materials. Kor. J Herbology 17 : 51-60
  20. Song J. H., M. J. Kim, H. D. Kwon, and I. H. Park, (2003). Antimicrobial activity of fractional extracts from Houttuynia cordata root, J. Korean Soc. Food Sci. Nutr., 32 : 1053-1058
  21. Scheuplein R. J., Blank IH. (1971). Permeability of the skin. Physiol Rev 51(4) : 702-747
  22. Yun M. E., Y. S. Lee, Y. J. Lee, Y. M. Park, and S. N. Park. (2018). Antimicrobial, Antioxidant and Cellular Protective Effects of Houttuynia cordata Extract and Fraction. Appl. Chem. Eng., 29(4) : 452-460