• Journal of Animal Science and Technology
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• v.48 no.3
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• pp.375-382
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• 2006

This study was conducted to investigate the effects of dietary herbal plant mixture on growth performance, blood immunological parameters, fecal VFA and NH3-N concentrations in growing pigs. The dietary treatments were 1) NC (negative control; antibiotics-free diet), 2) PC (positive control; NC diet added 0.16% antibiotic), 3) NCK0.2 (NC diet added 0.2% herbal plant mixture (koppuul??)) and 4) PCK0.1 (PC diet added 0.1% herbal plant mixture (koppuul??)). Eighty crossbred (Landrace×Yorkshire×Duroc) pigs (16.35±0.05kg average initial body weight) were used for 42 days. The pigs were assigned to the treatments according to body weight and each treatment had 5 replicates of 4 pigs per pen in a randomized complete block design. During the whole experimental period, PCK0.1 and PC treatments had significantly higher ADG than NC treatment (P<0.05). Gain/feed of pigs fed PC diet was higher than that of pigs fed NC diet (P<0.05). For blood immunological parameters investigations, white blood cells (WBC) counts and IgG level increased in the pigs fed PC, NCK0.2 and PCK0.1 diets compared to pigs fed NC diet. In addition, higher (P<0.05) serum lymphocyte concentration was found in PCK0.1 treatment compared to NC treatment. NH3-N concentration in pigs fed NCK0.2% diet was lower than that in pigs fed PC and NC diets. There were no significant differences in DM and N digestibilities among the treatments. In conclusion, the results suggested that the dietary additions of herbal plant mixture or antibiotics increase ADG, the concentrations of WBC counts and IgG, lymphocyte, while decrease NH3-N concentration in feces.

• Journal of Animal Science and Technology
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• v.50 no.4
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• pp.485-498
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• 2008

This study was conducted to evaluate effects of dietary rare earth on growth performance, blood immune- related cell population, meat quality and fecal odor emitting gases in finishing pigs. The total of sixty four (Landrace×Yorkshire×Duroc) pigs(65.42±1.16kg in average initial body weight) were used for feeding trial during 10 weeks of experimental period. Dietary treatments included 1) NC(antibiotic free diet), 2) PC (NC diet+6 weeks 44ppm of tylosin/ 4 weeks 22ppm of tylosin) 3) RE1 (NC diet + 100ppm of RE), 4) RE2 (NC diet+200ppm of RE). There were four dietary treatments with four replicate pens per treatment and four pigs per pen. During the overall periods, there were no significant differences in ADG(Average daily gain), ADFI (Average daily feed intake) and gain/feed ratio among treatments(P>0.05). Dry matter and nitrogen digestibility were higher in RE2 treatment group than other groups(P<0.05). Also, energy digestibility was higher in RE2 treatment group than PC and RE1 treatment groups(P<0.05). At the 6th week WBC(white blood cell) was significantly increased(P<0.05) in RE1 treatment group than NC and RE2 treatment groups. L* value of M. logissimus dorsi muscle color was significantly increased(P<0.05) in rare earth supplemented groups compared to NC treatment group(P<0.05). However, a* value was lower in RE1 treatment group than PC treatment group (P<0.05). In fatty acid composition of Intramuscular fat, total MUFA was significantly higher in RE2 treatment group than other groups(P<0.05). Also, total UFA was significantly increased in RE2 treatment group compared with NC and PC treatment groups(P<0.05). In fatty acid composition of back fats, total SFA of rare earth supplemented groups were lower than in PC treatment group(P<0.05). UFA:SFA ratio was significantly higher in rare earth supplemented groups than PC treatment group(P<0.05). In fecal odor emission, NH3 was significantly decreased(P<0.05) in rare earth supplemented groups compared to NC and PC treatment groups. In conclusion, the results of the experiment was affected by rare earth supplementation on digestibilities, meat quality, fatty acid and fecal odor emission gases in finishing pigs.

• Journal of Microbiology and Biotechnology
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• v.21 no.1
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• pp.28-36
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• 2011

Diblock copolymers composed of poly(${\varepsilon}$-caprolactone) (PCL) and poly(N,N-dimethylamino-2-ethyl methacrylate) (PDMAEMA), or methoxy polyethylene glycol(PEG), were synthesized via a combination of ring-opening polymerization and atom-transfer radical polymerization in order to prepare polymeric nanoparticles as an antifungal drug carrier. Amphotericin B (AmB), a natural antibiotic, was incorporated into the polymeric nanoparticles. The physical properties of AmB-incorporated polymeric nanoparticles with PCL-b-PDMAEMA and PCL-b-PEG were studied in relation to morphology and particle size. In the aggregation state study, AmB-incorporated PCL-b- PDMAEMA nanoparticles exhibited a monomeric state pattern of free AmB, whereas AmB-incorporated PCL-b- PEG nanoparticles displayed an aggregated pattern. In in vitro hemolysis tests with human red blood cells, AmBincorporated PCL-b-PDMAEMA nanoparticles were seen to be 10 times less cytotoxic than free AmB (5 ${\mu}g$/ml). In addition, an improved antifungal activity of AmBincorporated polymeric nanoparticles was observed through antifungal activity tests using Candida albicans, whereas polymeric nanoparticles themselves were seen not to affect activity. Finally, in vitro AmB release studies were conducted, proving the potential of AmB-incorporated PCL-b-PDMAEMA nanoparticles as a new formulation candidate for AmB.

• Journal of the Korean Data and Information Science Society
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• v.26 no.3
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• pp.669-675
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• 2015

This study was carried out to elucidate the effect of different rearing system on cortisol level, stress hormone, and fatty acid composition in the edible muscle tissues. These steers were reared in two different systems including antibiotic-free (ARS) and conservative system (CRS). In the M-Longissimus tissue, cortisol level was significantly lower in ARS than CRS, (p=0.0176). But, the levels of total saturated and unsaturated-fatty acids does not differ in ARS as CRS (p >0.05). However, the total saturated fatty acid levels tended to be greater in CRS and the total unsaturated fatty acid levels tended to be greater in ARS. However, the level of n-6 unsaturated fatty acid was higher in ARS than CRS (p=0.004). Especially, levels of linoleic acid (LA) and ${\gamma}$-linolenic acid (GLA) were significantly higher in ARS (p <0.01). Cortisol level and the n-6 fatty acid content in muscle tissue were negatively correlated (at p=0.00140.) In conclusion, ARS may produce beef with higher quality which contains lower cortisol and greater n-6 fatty acids, such as ALA and GLA.

• Journal of Veterinary Clinics
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• v.32 no.2
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• pp.191-195
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• 2015

A 7-year-old castrated male Korean Shorthair cat was referred with lethargy and anorexia. Laboratory examination revealed moderate degenerative changes of peripheral neutrophils on blood smear examination and decreased levels of free and total thyroxine ($T_4$) as well as bacterial growth on blood culture. Molecular analyses of the 16S ribosomal RNA gene and heat shock protein 60 gene confirmed the bacterium as Enterobacter cloacae. A minimal inhibitory concentration test showed multidrug resistance of the bacterium against 16 antibiotics. Polymerase chain reaction (PCR) and subsequent sequencing specifically for $bla_{TEM}$, $bla_{SHV}$, $bla_{CTX-M}$, and plasmid-mediated ampC genes revealed positive results to $bla_{TEM-1}$, $bla_{CTX-M-15}$, and plasmid-mediated $bla_{ACT-1}$ genes, indicating that the isolated bacterium contains plasmids containing genes encoding extended-spectrum beta-lactamase and plasmid-mediated ampC beta-lactamase. After 1 month of treatment with antibiotics and levothyroxine, the cat's condition improved; both the thyroid function test and the blood culture showed no abnormalities. This is the first report of community-acquired multidrug-resistant E. cloacae-induced euthyroid sick syndrome in a cat. By the prompt diagnostic procedures and properly selected antibiotic therapy, the cat was recovered from the multidrug-resistant bacterium-induced septicaemia.

• Journal of Microbiology and Biotechnology
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• v.34 no.7
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• pp.1484-1490
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• 2024

The gut microbiota is a key factor significantly impacting host health by influencing metabolism and immune function. Its composition can be altered by genetic factors, as well as environmental factors such as the host's surroundings, diet, and antibiotic usage. This study aims to examine how the characteristics of the gut microbiota in pigs, used as source animals for xenotransplantation, vary depending on their rearing environment. We compared the diversity and composition of gut microbiota in fecal samples from pigs raised in specific pathogen-free (SPF) and conventional (non-SPF) facilities. The 16S RNA metagenome sequencing results revealed that pigs raised in non-SPF facilities exhibited greater gut microbiota diversity compared to those in SPF facilities. Genera such as Streptococcus and Ruminococcus were more abundant in SPF pigs compared to non-SPF pigs, while Blautia, Bacteroides, and Roseburia were only observed in SPF pigs. Conversely, Prevotella was exclusively present in non-SPF pigs. It was predicted that SPF pigs would show higher levels of processes related to carbohydrate and nucleotide metabolism, and environmental information processing. On the other hand, energy and lipid metabolism, as well as processes associated with genetic information, cell communication, and diseases, were predicted to be more active in the gut microbiota of non-SPF pigs. This study provides insights into how the presence or absence of microorganisms, including pathogens, in pig-rearing facilities affects the composition and function of the pigs' gut microbiota. Furthermore, this serves as a reference for tracing whether xenotransplantation source pigs were maintained in a pathogen-controlled environment.

• Journal of Life Science
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• v.22 no.9
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• pp.1152-1158
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• 2012

Development of transgenic plant increasing crop yield or disease resistance is good way to solve the world food shortage. However, the persistence of marker genes in crops leads to serious public concerns about the safety of transgenic crops. In the present paper, we developed marker-free transgenic rice inserted high molecular-weight glutenin subunit (HMW-GS) gene ($D{\times}5$) from the Korean wheat cultivar 'Jokyeong' using Agrobacterium-mediated co-transformation method. Two expression cassettes comprised of separate DNA fragments containing only the $D{\times}5$ and hygromycin resistance (HPTII) genes were introduced separately into Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring $D{\times}5$ or HPTII was infected into rice calli at a 3: 1 ratio of EHA105 with $D{\times}5$ gene and EHA105 with HPTII gene expressing cassette. Then, among 66 hygromycin-resistant transformants, we obtained two transgenic lines inserted with both the $D{\times}5$ and HPTII genes into the rice genome. We reconfirmed integration of the $D{\times}5$ and HPTII genes into the rice genome by Southern blot analysis. Wheat $D{\times}5$ transcripts in $T_1$ rice seeds were examined with semi-quantitative RT-PCR. Finally, the marker-free plants containing only the $D{\times}5$ gene were successfully screened at the $T_1$ generation. These results show that a co-infection system with two expression cassettes could be an efficient strategy to generate marker-free transgenic rice plants.

• Korean journal of applied entomology
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• v.18 no.1 s.38
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• pp.55-61
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• 1979

Since rice high yielding practices with blast resistant varieties induced severe and earlier infestation of sheath blight recently, it is strongly suggested to spray at least 10 days earlier than the present recommendation and more than 2 sprays from early July to early August seems to be essential for the efficient control of sheath blight in the future. Among organo-arsenic fungicides, MAFe (Neo Asozin) is known as the most effective and economic as well as with the advantages of free phytotoxicity and safe residure on rice, and applications of the mixed formulation with other fungicide and insecticide is suitable for the one-hit control of rice diseases and insect pests in connection with cooperative mass control system. Present level of arsenic $(As_2O_3)$ residue in .ice is summarized as 0.2ppm to 0.65 ppm in highest average, and there are more possibilities of extensive MAFe fungicide uses for the efficient and economic control of this disease. During the past few years, the acreage of sheath blight control has been extended several times while the increase of the fungicides used for sheath blight showing about 10 times in quantity and mort than 15 times in amount, and also the fungicide cost estimated for the control is differ from 1 to 12 billion 1on by the use of MAFe or antibiotic fungicide in a year.

• The Korean Journal of Food And Nutrition
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• v.16 no.3
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• pp.187-191
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• 2003

It was performed to find out difference of the nutritional contents between chungjung chicken meats grown chemicals free and general chicken meats. The chungjung chicken was eat and given feeds of good quality mixed living microbial agent, power of ginseng leaves and by-product of extract liquefied sea foods from fishes producted clean area of the Western Sea, including plentiful calciums, amino acids, docosahexanoic acid(DHA) etc. Addition of they improved chungjung chicken yields and meat quality compared to general chicken given feeds mixed antibiotic etc. Also, chungjung chickens eaten miced type develop resistance to in disease. There were significant trend in crude proteins, calciums, crude lipids contents, and unsaturated fatty acids between the chungjung chicken meats and the general chicken meats. The crude proteins and calciums contents were higher in the chungjung chicken meats than the general chicken meats, but the crude lipids contents were higher in the general chicken meats than that. Also, contents of the n6 fatty acids and n3 fatty acids in chungjung meats were higher than that. Especially, contents of DHA in the chungjung chicken meats were detected higher than the general chicken meats. Therefore, the results from this experiments will provide a information for the nutritional knowledge of the 'white meats', especially the clean chicken meats.

• Korean Chemical Engineering Research
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• v.48 no.6
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• pp.690-694
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• 2010

In this study, herbal wood vinegar including Bambusoideae, Cinnamomi Cortex, Zingiberis Rhizoma was tested to see possibility for cosmetic or skin related medicine. Anti-oxidation effect of herbal wood vinegar was tested by DPPH free radical scavenging activity, and showed 97% inhibition rate at $50{\mu}g/ml$. Anti-bacterial effect was tested by disc diffusion method, and it indicated strong anti-bacterial activity against normal skin flora Staphylococcus aureus. Whitening effect was measured by tyrosinase inhibition assay, and it was lower compared with vitamin C. Stability test was done by MTT assay, and cell toxicity was relatively high. Stability was also checked, and there was not significant change in color, aroma, appearance and pH during storage. Anti-atopic dermatitis test was done by hairless mouse and herbal wood vinegar recovered damaged skin to almost normal condition after 9 days of application. IgE concentration in herbal wood vinegar treated mouse was also reduced 30% compared with control. From the study, herbal wood vinegar showed good anti-oxidation, anti-bacterial and anti-atopic dermatitis effect, and had promising application in cosmetic or skin related medicine.