• Applied Chemistry for Engineering
• /
• v.35 no.4
• /
• pp.335-340
• /
• 2024

In this paper, we synthesized organic and inorganic hybrid materials to introduce antibody functionality to UIO-66 and incorporated them into a surface plasmon resonance (SPR) assay to enhance the sensitivity of detecting small molecules such as oxytocin. A biological marker peptide called oxytocin may help in the diagnosis of heart failure, Alzheimer's disease, and cancer. To detect oxytocin at concentrations as low as a few femtomole (fM), we developed a surface sandwich assay utilizing a pair of oxytocin-specific antibodies for enhancing selectivity and one of metal organic frameworks [e.g., UiO-66-(COOH)₂] possessing high porosity and surface-area as a signal amplifier. Initially, real-time SPR assays were used to confirm that each selected oxytocin-specific antibody binds strongly to oxytocin and to different binding sites on oxytocin. One of these antibodies (e.g., anti-OXT[OTI5G4]) was immobilized on the surface of a thin gold chip. Upon sequential injecting of oxytocin and the other antibody (e.g., anti-OXT[4G11]) conjugated to UiO-66-(COOH)₂ onto the surface to form the surface sandwich complex of anti-OXT[OTI5G4]/oxytocin/UiO-66-(COOH)₂-anti-OXT[4G11]), SPR changes, which varied with oxytocin concentration, were then measured in real time. The results demonstrated that sensitivity was amplified by over a million-fold compared to assays without UiO-66-(COOH)₂, enabling oxytocin detection down to approximately 10 fM.

• Journal of applied mathematics & informatics
• /
• v.32 no.1_2
• /
• pp.267-284
• /
• 2014

The multiresolution analysis for Legendre multiwavelets are given, anti-derivatives of Legendre multiwavelets are used for the numerical solution of differential equations, a special form of multilevel augmentation method algorithm is proposed to solve the disrete linear system efficiently, convergence rate of the Galerkin methods is given and numerical examples are presented.

• Korean Institute of Interior Design Journal
• /
• no.2
• /
• pp.40-45
• /
• 1994

This thesis is to research the change in attitude regarding the architectura ornament. One of the key dogmas within Modern Movement of art and design was anti-ornamentalism. The modernist had revolted against ornament and believed that the greatness of the modern age might lay in its inability to produce a new form of ornament. Architectural ornament has been passed through an process of evolution in a decadent stage. In this context, the purpose of this study is to identify historical meaning of architectural ornament in the age of transition toward Modernism.

• Proceedings of the Microbiological Society of Korea Conference
• /
• 2007.05a
• /
• pp.94-94
• /
• 2007

• Proceedings of the Korean Society of Toxicology Conference
• /
• 2001.10a
• /
• pp.133-134
• /
• 2001

Abnormal regulation of the inducible form of cyclooxygenase (COX-2) has been often observed in various types of cancerous and transformed cells. Recently, targeted inhibition of COX-2 is recognized as one of the promising strategies for the prevention or treatment of cancer as well as inflammation, As part of a program to evaluate the cancer chemopreventive potential of anti-inflammatory phytochemicals, we initially determined the COX-2 inhibitory activity of some naturally occurring diarylheptanoids structurally related to curcumin.(omitted)

• The Transactions of the Korean Institute of Electrical Engineers
• /
• v.43 no.3
• /
• pp.447-457
• /
• 1994

This paper presents a compensation method for discrete-time control systems with saturation nonlinearities to cope with the reset windup phenomena. The proposed ARW (Anti-Reset Windup) method is motivated by the concept of the equilibrium point. The design parameter of the ARW scheme is explicitly derived by minimizing a reasonable performance index. The resulting dynamics of the compensated controller exhibits the reduced model form of the unsaturated system which can be obtained by the singular perturbational model reduction method. An example is given to illustrate the effectiveness of the proposed method.

• Proceedings of the Korean Nutrition Society Conference
• /
• 2001.05a
• /
• pp.310.2-310
• /
• 2001

• Archives of Pharmacal Research
• /
• v.6 no.1
• /
• pp.75-77
• /
• 1983

Two phenolic acids were isolated from the hydrolyzed butanol fraction of Aralia continenetalis K. in crystalline form. They were identified by chemical tests and by analysis of spectra of UV, IR and NMR as ferulic acid and caffeic acid.

• Journal of the Korean Chemical Society
• /
• v.59 no.3
• /
• pp.209-214
• /
• 2015

The formation of complexes between SO₂ and acetic acid was studied theoretically. The ab initio and DFT calculations were performed with MP2 and B3LYP methods using 6-311++G(d,p), aug-cc-pVDZ and aug-cc-pVTZ basis sets. Six stable complexes were identified, and three stable bidentate complexes, C1, C2 and C3, were formed between SO₂ and syn-acetic acid, which is more stable form of acetic acid. Anti-acetic acid also form three complexes, C4, C5 and C6, with SO₂. C4 is bidentate and C5, C6 are monodentate complexes, which are less stable. The most stable complex, C1 has S⋯O=C and O⋯H-O interactions, and the S⋯O and O⋯H distances are less than the sum of van der Waals radii. The vibrational frequencies of complexes were calculated and were compared with those of monomers. The frequency shifts after formation of complex were found, and the overall pattern of frequency shifts relative to monomers is similar among the six complexes.

• The Korean Journal of Nuclear Medicine
• /
• v.36 no.2
• /
• pp.133-139
• /
• 2002

Purpose: Scintillation Proximity Assay (SPA) does not require the physical separation of receptor bound form from free form. SPA was applied to the study of interaction of human chorionic gonadotropin (hCG) and $anti-{\alpha}$ hCG in serum. Materials and methods: $Anti-{\alpha}$ hCG was biotinylated for the binding to streptavidin. The assay was based on the simple competitive binding method between $[^{125}I]hCG$ and the hCG in sample serum, with $anti-{\alpha}$ hCG-coated beads. Aliquots of biotinylated $anti-{\alpha}$ hCG were dispensed into scintillation vials containing $100{\mu}{\ell}\;[^125}I]hCG\;and\;200{\mu}{\ell}$ of either a standard concentration of hCG for preparation of standard curve or unknown sample, and incubated for 20 min. at room temperature. Then $20{\mu}{\ell}$ streptavidin-coated beads were added to vials, and finally incubated for 10 min at room temperature. Values for unknown samples were then calculated from the standard curve. Results: Optimal background counts were certificated using varied radioactivity of radionuclides. Appropriate standard curve was obtained from SPA method successively, and the concentration of hCG from unknown serum was determined by standard curve. The result from SPA assay was similar to that of RIA. Conclusion: This observation confirms that SPA method could be useful for clinical diagnosis.