• 한국독성학회:학술대회논문집
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• 한국독성학회 2001년도 International Symposium on Dietary and Medicinal Antimutgens and Anticarcinogens
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• pp.133-134
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• 2001

Abnormal regulation of the inducible form of cyclooxygenase (COX-2) has been often observed in various types of cancerous and transformed cells. Recently, targeted inhibition of COX-2 is recognized as one of the promising strategies for the prevention or treatment of cancer as well as inflammation, As part of a program to evaluate the cancer chemopreventive potential of anti-inflammatory phytochemicals, we initially determined the COX-2 inhibitory activity of some naturally occurring diarylheptanoids structurally related to curcumin.(omitted)

• 대한전기학회논문지
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• 제43권3호
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• pp.447-457
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• 1994

This paper presents a compensation method for discrete-time control systems with saturation nonlinearities to cope with the reset windup phenomena. The proposed ARW (Anti-Reset Windup) method is motivated by the concept of the equilibrium point. The design parameter of the ARW scheme is explicitly derived by minimizing a reasonable performance index. The resulting dynamics of the compensated controller exhibits the reduced model form of the unsaturated system which can be obtained by the singular perturbational model reduction method. An example is given to illustrate the effectiveness of the proposed method.

• 한국영양학회:학술대회논문집
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• 한국영양학회 2001년도 춘계연합학술대회 초록
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• pp.310.2-310
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• 2001

• Archives of Pharmacal Research
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• 제6권1호
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• pp.75-77
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• 1983

Two phenolic acids were isolated from the hydrolyzed butanol fraction of Aralia continenetalis K. in crystalline form. They were identified by chemical tests and by analysis of spectra of UV, IR and NMR as ferulic acid and caffeic acid.

• 대한화학회지
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• 제59권3호
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• pp.209-214
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• 2015

Acetic acid와 SO₂의 상호작용에 의해 생성되는 안정한 복합체를 알아보기 위해 MP2, B3LYP 방법으로 6-311++G(d,p), aug-cc-pVDZ, aug-cc-pVTZ basis set을 사용하여 계산하였다. Syn-acetic acid와 SO₂는 세 종류의 안정한 두 자리 복합체를 형성하였으며 anti-acetic acid도 SO₂와 세 복합체를 보였으나 이들 중 둘은 한자리 복합체로 syn-acetic acid에 비해 안정성이 떨어진다. 가장 안정한 복합체는 SO₂의 S와 syn-acetic acid의 C=O가 상호작용하여 S⋯O=C 결합과 SO₂의 O와 acetic acid의 O-H가 상호작용하여 O⋯H-O 결합을 갖는 두 자리 복합체, C1, C3이다. 이중 C1이 가장 큰 결합에너지 −7.38 kcal/mol을 갖는다. 이 복합체의 안정성은 S⋯O와 O⋯H 거리가 van der Waals 반지름의 합보다 훨씬 짧다는 것으로도 알 수 있다. Vibrational frequencies의 변화에서도 이 원자들의 상호작용을 볼 수 있었다.

• 대한핵의학회지
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• 제36권2호
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• pp.133-139
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• 2002

목적: 섬광 근접측정법은 항원 항체 반응 후 결합 분획과 유리분획을 분리하는 과정이 필요없다. 이러한 원리를 검체 내 hCG와 항 ${\alpha}$ hCG 항체간의 항원 항체 반응에 적용하고자 한다. 대상 및 방법: 항 ${\alpha}$ hCG 항체를 biotin과 결합시켜 SPA bead에 부착된 streptavidin과 부착 가능하게 만들었다. 이 측정법은 항 ${\alpha}$ hCG 항체가 부착된 SPA beads에 대해 혈청내 hCG와 표지항원인 $[^{125}I]hCG$간의 경쟁 반응을 기본 원리로 이용하였다. Biotin 표지 항 ${\alpha}$ hCG 항체를 $[^{125}I]hCG\;100{\mu}{\ell}$와 표준용액이나 환자 혈청 $200{\mu}{\ell}$이 들어있는 실온에서 20분 방치하였다. 그리고 streptavidin이 붙은 SPA beads $20{\mu}{\ell}$를 바이알에 넣고 10분 더 방치한다. 환자 혈청의 수치를 표준 응답곡선을 통해 계산하였다. 결과: SPA측정법에 사용되는 방사성 핵종의 방사능 양에 따라 반응용액 속에서 SPA bead와 자유 방사성 핵종에 의한 배후 방사능이 측정값에 영향이 없음을 확인하였다. SPA 방법을 응용한 측정에서 적합한 표준 응답곡선을 얻었고, 실제 환자혈청에서의 hCG 농도를 결정할 수 있었다. 결론: 이 실험을 통해 SPA 방법을 이용한 측정법이 임상진단에 유용하게 사용될 수 있을 것으로 사료된다.

• 수산해양기술연구
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• 제18권1호
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• pp.35-38
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• 1982

이상(以上)과 같은 고제(考祭)을 통(通)하여 다음과 같은 결론(結論)을 얻을수 있었다. 1. 각진동차수별(各振動次數別)로 상부구조물(上部構造物)의 길이 변화(變化)에 대한 고유진동수(固有振動數)의 변화(變化)경향을 알수 있다. 2. 같은 구조물(構造物)이던 홀쭉할수록 고유진동수(固有振動數)가 커지고, 커지는 경향은 고차(高次)일수록 터 커진다. 3. 본고(本槁)에서는 두가지 선형(船型)에만 대하여 전달(傳達) matrix 해석법(解析法)으르 검토 고찰 하였으나 그 계산용량(計算容量)이바른 해석법(解析法)보다 훨씬 적음을 감안할때, 본방법(本方法)에 의하여 여러 선형(船型)에 대한 진동자료(振動資料)를 계통적(系統的)으로 계산(計算)하여 두면 방진(防振)자료에 큰 도움이 될것으로 기대된다.

• 대한핵의학회지
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• 제18권1호
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• pp.19-24
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• 1984

Clinical measurement of thyroid autoantibodies in sera of some thyroid disorders have been widely applied since about twenty years ago. We investigated the incidence and titers of both anti microsomal and antithyroglobulin antibodies in forty eight cases with controls and one hundred and thirty three patients with some form of thyroid disorders. The results were as follows; 1) In controls, antimicrosomal antibodies were positive in 2% but anti thyroglobulin antibodies were all negative. 2) In a series of one hundred and thirty three patients with thyroid disease, anti microsomal antibodies were positive in 44% but antithyroglobulin antibodies were positive in only 15%. 3) The rate disclosing the positive results of antimicrosomal antibodies were 71 % in Hashmoto's disease, 60% in Graves' disease, and 38% in primary hypothroidism, respectively. On the other hand, the positive results of antithyroglobulin antibodies showed 21 % in Graves' disease, 19% in primary hypothyroidism, and 18% in Hashmoto's disease, respectively. Though there were relatively high rate of both antimicrosomal and anti thyroglobulin antibodies in patients with nodular goiter, they were only seven cases in our series. 4) The rate with the extremely high titers of antimicrosomal and antithyroglobulin antibodies$(>1:160^2)$ was 83% and 67% in Hashmoto's disease, 50% and 67% in primary hypothyroidism, and 41% and 18% in Graves' disease. Accordingly, the thyroid autoantibodies were commonly found higher positive rate in patients with Hashmoto's disease, primary hypothyroidism, and Graves' disease. Among these disorders, the extremely high positive rate of the thyroid autoantibodies was found in patients with Hashmoto's disease.

• Journal of Microbiology and Biotechnology
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• 제11권6호
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• pp.1093-1098
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• 2001

The gene coding for a Lepidoptera-specific insecticidal crystalline (or control) protein (ICP), recognized as cryIAc, from Bacillus thuringiensis subsp. kurstaki HD-73, was cloned into the vector pBluscript ll SK-, and then transformed in Escherichia coli $DH5{\alpha}$. The clone was named EBtIAc and the chimeric phagemid, as pEBtIAc. Hyperexpression of CryIAc protoxin was observed in the extract of the culture of E. coli harboring pEBtIAc. Crystalline protoxin was purified by differential solubility. It was dissolved in alkaline pH, and exposed to trypsin to be activated. The molecular weights of the pro- and activated toxins on SDS-PAGE were estimated to be ca. 130 kDa and 60 kDa, respectively. The toxicity was tested by force-feeding larvae of gypsi moth (Lymantria diapar) with trypsinized protoxin. Using the batch of biologically active form of the toxin as an immunogen, anti-CryIAc antiserum was raised in a New Zealand white rabbit. Immunoglobulin G was fractionated from the seam by Protein-A sepharose affinity chromatography. Immunoreactivity of the antibody was examined by dot and Westerns blottings. It has been found that the anti- CryIAc antibody recognized the purified toxin at a level below a nanogram in terms of quantity. Using the antibody some of Bt-corns were able to be differentiated from tons of corn kernels which were imported from America as forage crops.

• The Korean Journal of Physiology and Pharmacology
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• 제16권5호
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• pp.361-365
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• 2012

Anti-tumor activity of the proteins from Gecko (GP) on cervical cancer cells, and its signaling mechanisms were assessed by viable cell counting, propidium iodide (PI) staining, and Western blot analysis. GP induced the cell death of HeLa cells in a dose-dependent manner while it did not affect the viability of normal cells. Western blot analysis showed that GP decreased the activation of Akt, and co-administration of GP and Akt inhibitors synergistically exerted anti-tumor activities on HeLa cells, suggesting the involvement of PI3-kinase/Akt pathway in GP-induced cell death of the cancer cells. Indeed, the cytotoxic effect of GP against HeLa cells was inhibited by overexpression of constituvely active form of Akt in HeLa cells. The candidates of the functional proteins in GP were analyzed by Mass-spectrum. Taken together, our results suggest that GP elicits anti-tumor activity against HeLa cells by inhibition of PI3-kinase/Akt pathway.