• Archives of Pharmacal Research
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• 제16권4호
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• pp.347-348
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• 1993

• 대한핵의학회지
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• 제29권1호
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• pp.98-104
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• 1995

필자는 서울대학교 의과대학 병리학 교실에서 만든 항 백혈병 항체(항 CALLA 항체, 항 JL-1 항체)에 대해 $^{99m}Tc$과 $^{125}I$의 표지 방법 및 면역학적 성상을 연구하여 임상 응용의 토대를 만들고자 하였다. $^{99m}Tc$은 pretargeting transchelation법으로 $^{125}I$는 lodogen법으로 표지하였다. 각 항체에 대해 결합 반응검사, Scatchard 분석, 변조 현상 검사를 시행하였다. $^{125}I$는 두 항체 모두에서 90%전후의 높은 표지율을 보인 반면, $^{99m}Tc$ 경우 70%이하의 표지율을 보여 개선점이 요구되었다. 결합반응검사에서도 $^{99m}Tc$을 표지한 항체는 30%정도의 낮은 면역 반응성을 보였다. Scatchard 분석 결과 결합상수 모두가 $10^9$으로 좋은 친화력을 보였고, 세포당 항체 결합 수는 $10^4$으로 비교적 높은 농도로 나타났다. 변조 현상은 모든 경우에서 나타나지 않았다. 항 CALLA 항체, 항 JL-1 항체는 향후 림프종과 백혈병의 진단 및 치료에 도움이 될 수 있으리라 기대된다.

• Archives of Pharmacal Research
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• 제17권6호
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• pp.452-457
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• 1994

The water soluble long-chain crosslinker, sulfo-succinimidyl-6-[3'-(2-pyridyldithio)-propion-amido]hexanoate (S-LC-SPDP) was used to prepare ricin A chain (RAT) immunotoxins constructed with whole igG and Fab fragments of the anti-common acute lymphoblastic leukemiz antigen (CALLA)monoclonal antibody. In this study, a) S-LC-SPDP modification efficiencies immunoreactivity and cytotoxicity of immunotoxins constructed were examined. IgG-RTA and Fab-RTA immunotoxins were prepared with 67.3% and 57.0% conjugation yields, respectively. These long spacer intemolecular linked immunotoxins were selectively immunoreactive and to antigen K562 cells. Both IgG-RAT and Fab-RAT immunotoxins were 210-and 45-fold more active than intavt RAT in vitro, respectively.

• Archives of Pharmacal Research
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• 제22권5호
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• pp.459-463
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• 1999

The effects of the hindered and non-hindered water soluble long-chain disulfide bonds on the stability and cytotoxicity of the ricin A chain (RTA) immunotoxin were examined. The RTA immunotoxins were prepared with the Fab fragments of anti-common acute lymphoblastic leukemia antigen (CALLA) monoclonal antibody (Fab-RTA) using sulfosuccinimidyl-6-[(-methyl-(-2-pyridyldithio)toluamido]toluamido]hexanoate (S-LC-SMPT) and sulfosuccinimidyl-6-[3-(2-pyridyldithio-propionamido]hexanoate (S-LC-SPDP). The prepared Fab-RTA immunotoxins were evaluated for their conjugation yield, immunoreactivity, thermal and disulfide bond stability and cytotoxicity. The conjugation yield of the Fab-RTA immunotoxin from the water soluble long chain crosslinking agents, S-LC-SMPT and S-LC-SPDP, were comparable. Both Fab-RTA immunotoxins exhibited a similar immunoreactivity and thermal stability in aqueous solution. However, S-LC-SMPT -mediated Fab-RTA, sterically hindered, showed an enhanced disulfide bond stability in vitro over S-LC-SPDP mediated one. In the cytotoxicity against antigenic cell Daudi, the S-LC-SMPT -mediated RTA immunotoxin maintained a comparable cytotoxicity, compared with S-LC-SPDP mediated Fab-RTA immunotoxin.