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The Pharmaceutical Society of Korea (대한약학회)
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Ricin A Immunotoxins of IgG and Fab of Anti-CALLA Monoclonal Antibody: Effect of Water Soluble Long-chain SPDP on Conjugate Yield, Immunoselectivity and Cytotoxicity

  • Published : 1994.12.01
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Abstract

The water soluble long-chain crosslinker, sulfo-succinimidyl-6-[3'-(2-pyridyldithio)-propion-amido]hexanoate (S-LC-SPDP) was used to prepare ricin A chain (RAT) immunotoxins constructed with whole igG and Fab fragments of the anti-common acute lymphoblastic leukemiz antigen (CALLA)monoclonal antibody. In this study, a) S-LC-SPDP modification efficiencies immunoreactivity and cytotoxicity of immunotoxins constructed were examined. IgG-RTA and Fab-RTA immunotoxins were prepared with 67.3% and 57.0% conjugation yields, respectively. These long spacer intemolecular linked immunotoxins were selectively immunoreactive and to antigen K562 cells. Both IgG-RAT and Fab-RAT immunotoxins were 210-and 45-fold more active than intavt RAT in vitro, respectively.

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References

  1. J. Clin. Immunol. v.12 Targeted kill: From umbrellas to monoclonal antibodies Byers,V.S.;Baldwin,R.W.
  2. Biochem J. v.173 Protein thiolation and reversible protein-protein conjugation: N-succinimidyl 3-(2-pyridyldithiol)propionate, a new hterobifuncational reagent Carlsson,J.;Drevin,H.;Axen,R.
  3. Arch. Pharm. Res. v.16 Cytotoxicity of anti-CALLA monoclonal antibody conjugates to methtotrexate Chun,C.J.;Lee,K.C.
  4. J. Immunol. v.141 Comparison of the cytotoxic potency of T101 Fab, F(ab')₂and whole lgG immunotoxins Derocq,J.M.;Casellans,P.;Laurent,G.;Ravel,S.;Vidal,H.;Jansen,F.
  5. Proc. Natl. Acad. Sc. USA v.80 Enhancement of toxicity of anti-trasfemin receptor antibody-Psedomonas exotoxin conjugates by adenovirus Fitzgerald,D.J.P.;Trowbride,I.S.;Pastan,I.;Willingham,M.C.
  6. Cancer Chemo. Pharmacol. v.24 Comparison of tetrazolium colorimetric and [³H]-uri-dine assays for in vitro chemosensitivity testing Ford,C.H.J.;Richardson,V.J.;Tsaltas,G.
  7. J. Immunol. v.136 The effect of antibody valency and lysosomotropic amines on the synergy between ricin A chain-and rich B chain-containing immunotoxins Fulton,R.J.;Uhr,J.W.;Vitetta,E.S.
  8. Inter. J. Pharm. v.59 Monoclonal antibody-based targeting of methdtrexateloaded microspheres Lee,K,C.;Lee,Y.J.;Kim,W.B.;Cha,C.Y.
  9. Seminars in Cell Biology Ribosome inactivating proteins of plants Lord,J.M.;Hartley,M.R.;Roberts,L.M.;Lord,J.M(ed.)
  10. J. Immounol. v.140 A flexible peptide spacer increase the efficacy of holoricin anti-T cell immunotoxins Marsh,J.W.;Neville,D.M.Jr.
  11. Biochemistry v.12 Pihl, Different biological properties of the two constituent peptide chains of ricin Olsnes,S.
  12. Eur. J. Biochem. v.104 Increased toxicity of Diphtheria toxin for human lymphoblastoid cells following covalent linkage to anti-(human lymphocyte) globulin or its F(ad')₂fragment Ross,W.C.J.;Thorpe,P.E.;Cumber,A.J.;Edwards,D.C.;Hinson,C.A.;Davies,J.S.
  13. J. Immunol. Methods. v.76 One-step purification of mouse monoclonal antibodies from ascites fluid by Hydroxylapatite Chromatography Stanker,L.H.;Vanderlaan,M.;Juarez-Salinas,H.
  14. J. Biol. Chem. v.255 Gelonin, a new inhibitor of protein synthesis, nontoxic to intact cells, isolation, chracterization, and preparation of cytotoxic complexes with concanavalin A Stripe,F.;Olsnes,S.;Pihl,A.
  15. Clin. Exp. Immunol. v.43 Abrogation of the non-specific toxicity of abrin conjugated to anti-lymphocyte globulin Thorpe,P.E.;Cumber,A.J.;Wiliams,N.;Edwards,D.C.;Ross,W.C.J.;Davis,A.J.S.
  16. Leukemia and Lymphoma v.9 Effect of the intermolecular toxin-monoclonal antibody linkage on th in vivo stability, immunogenicity and anti-leukemic activity of B43 (Anti-CD19)pokeweed antiviral protein immunotoxin Uckun,F.M.;Myers,D.E.;Irvin,J.D.;Kuebelbek,V.M.;Finnegan,D.;Chelstrom,L.M.;Houston,L.L.
  17. J. Biol. Chem. v.257 Kinetics of protein synthesis inhibition by ricin anti-Thy 1.1 monoclonal antibody hybrids Youle,R.J.;Neville,D.M.Jr.