Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Determination of Omeprazole in Rat Plasma by HPLC with Column Switching

  • Shim, Sang-Ho (College of Pharmacy, Chung-Nam National University) ;
  • Bok, Soo-Jin (College of Pharmacy, Chung-Nam National University) ;
  • Kwon, Kwang-Il (College of Pharmacy, Chung-Nam National University)
  • Published : 1994.12.01
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Abstract

A new high-performance liquid chromatographic method with column switching has been onto a Bondapak phenyl/corsil $(37-50{\;}{\mu}m)$ precolumn and polar plasma components were washed with 0.06 M borate burffer. After valve switching, the concentrated drug were eluted in the back-flush mode and separated on a ${\mu}-Bondapak$ C18 column with acetonitrilke-phosphate buffer as the mobile phae. The method showed excellent precision, accuracy and speed with detection limit of $0.01{\;}{\mug}/ml^{-1}$. Total analysis time per smaple was less than 20 min and the coefficients of variation for intra and inter-assay were less than 5.635. This method has been successfully applied to plasma smaples from eats after oral administration of omeprazole.

Keywords

References

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