• Bulletin of the Korean Chemical Society
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• v.35 no.10
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• pp.3015-3020
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• 2014

The behavior of benzo[a]pyrene-7,8-dione (BPQ) in aqueous solution and its interaction with native DNA was investigated using conventional absorption and linear dichroism (LD) spectroscopy. The appearance of a broad absorption maximum at long wavelengths and its proportional relationship to solvent polarizability suggested that BPQ adopts a aggregated state for all solutions examined. Disappearance of this absorption band at higher temperatures in aqueous solution also supported BPQ aggregation. When associated with DNA absorption spectral properties were essentially the same as that in aqueous solution. However, two isosbestic wavelengths were found in the concentration-dependent absorption spectrum of the BPQ-DNA complex, suggesting the presence of at least two or more DNA-bound BPQ species. Both species produced $LD^r$ spectra whose magnitude in BPQ absorption region is larger or comparable to that in the DNA absorption region, suggesting that the molecular BPQ plane is near perpendicular relative to the local DNA helical axis. Therefore, BPQ molecules are aligned along the DNA stem in both DNA-aggregated BPQ species.

• Bulletin of the Korean Chemical Society
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• v.29 no.2
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• pp.352-356
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• 2008

An analytical method has been developed for measuring chlorostyrenes in fish tissue sample. Extraction of chlorostyrenes from fish tissue was carried out by ultrasonication using acetone/n-hexane (5:2, v/v) mixture. Most of the lipids in the extract were eliminated by freezing-lipid filtration, prior to solid-phase extraction (SPE) cleanup. During freezing-lipid filtration, about 90% of the lipids extracted from the fish samples were easily removed without any significant losses of chlorostyrenes. For purification, SPE using Florisil was used for the rapid and effective cleanup. Quantification was performed using gas chromatography-mass spectrometry in the selected ion monitoring mode. Spiking experiments were carried out to determine the recovery, precision, and limits of detection (LODs) of the method. The overall recovery was above 80% in the spiked fish tissue sample at 10 and 100 ng/g levels, respectively. The detection limits for chlorostyrenes were ranged from 0.05 to 0.1 ng/g. This developed method is demonstrated to give efficient recoveries and LODs for detecting chlorostyrenes spiked into fish tissue with high lipid content.

• Korean Journal of Microbiology
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• v.27 no.1
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• pp.35-42
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• 1989

The mode of transglycosylation reaction observed during the action of low-molecular-weigh $\beta$-D-glucosidase ($\beta$-D-glucoside glucohydrolase, EC3.2.1.21) purified from Trichoderma koningii ATCC 26113 was investigated using $^{1}H$-NMR spectroscopy. The enzyme was purified by the series of procedures including ammonium sulfate precipitation, and fractionations by column chromatographies on Bio-Gel P-150, DEAE-Sephadex A-50, and SP-Sephadex C-50. The final purification was performed by the band eluation after preparative polyacrylamide gel electrophoresis. The enzyme showed its molecular size of 78,000 through the analysis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and its isoelectric point of 5.80 through the analysis of analytical isoelectric focusing. The H-1 proton resonances were analyzed. After the reaction of the enzyme with cellobiose, the reaction products were separated by high performance liquid chromatography using refractive index detector. H-1 resonances of the products were consisted with those of gentiobiose [$\beta$-D-glucopyranosyl--(1,6)-D-glucopyranose], and cellotriose [$\beta$-D glucopyranosyl-(1,4)-$\beta$-D-glucopyranosyl]-(1,4)-D-glucopyranose] with minor resonances of sophorose [$\beta$-D-glucopyranosyl-(1,2)-D-glucopyranose], respectively.

• Magazine of the Korean Society of Agricultural Engineers
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• v.38 no.3
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• pp.70-81
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• 1996

Solutions of geotechnical engineering problems require predictions of deformation and stresses during various stages of loading. Powerful numerical methods are available to make such predictions even for complicated problems. To get accurate results, realistic stress-strain relationships of soils are dependent on a number of factors such as soil type, density, stress level and stress path. Attempts are continuously being made to develope analytical models for soils incorporating all such factors. Isotropic compression-expansion test and a series of drained conventional triaxial tests with several stress path for Baekma river sand were performed to investigate stress-strain and volume change characteristics of Lade's single work hardening model dependant on the stress path. In order to predicted of stress-strain and volumetric strain behavior were determined the values of parameters for the mode by the computer program based on the regression analysis. Predicted stress-strain behavior of triaxial compression tests and optional stress path tests for increasing confining pressure with parameters obtained conventional triaxial compression tests agreed with several test results but the prediction results for decreasing confining pressure reduced triaxial compression tests make a little difference with test results.

• Bulletin of the Korean Chemical Society
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• v.32 no.3
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• pp.977-980
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• 2011

A new analytical method for measurement of 3,4-dihydroxyphenylalanine (DOPA) in human urine was developed. DOPA from an aqueous solution was converted into an ethoxycarbonyl (EOC) derivative. A tertbutyldimethylsilyl (TBDMS) reaction under anhydrous conditions was then attempted for analysis by gas chromatography-mass spectrometry in selected ion monitoring mode. A new mass spectral data on DOPA as a tri-EOC/mono-TBDMS derivative was built. This method showed good linearity (r ${\geq}$ 0.999), precision (% relative standard deviation = 3.1-9.2), and accuracy (% relative error = -7.2-8.8), with a detection limit of 0.05 ng/mL. This selective and accurate method of DOPA analysis will be useful for biochemical monitoring of various neurological disorders including Parkinson's disease in biological fluids.

• Computational Structural Engineering
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• v.2 no.2
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• pp.62-72
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• 1989

This study presents rational methods for probability-based estimates of basic design wind speeds in Korea and proposes a risk-based nation-wide map of design wind speeds. The paper examines the fittings of the extreme Type I mode to largest yearly non-typhoon wind data from long-term records, and to largest monthly non-typhoon wind data from short-term records. For the estimation of the extreme typhoon wins speed distribution, an indirect analytical method based on a Monte-Carlo simulation is applied to typhoon-prone regions. The basic desig wind speeds for typhoon and non-typhoon winds at the sites of concern are made to be obtained from the mixed model given as a product of the two distributions. The results of this study show that the proposed models and methods provide a practicable tool for the development of the risk-based basic design wind speed and the design wind map from short-term station records currently available in Korea.

• Structural Engineering and Mechanics
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• v.56 no.6
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• pp.939-957
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• 2015

In this paper, a new approach based on the continuum model is proposed to estimate the main cable tension force of suspension bridges from measured natural frequencies. This approach considered the vertical vibration of a main cable hinged at both towers and supported by an elastic girder and hangers along its entire length. The equation reflected the relationship between vibration frequency and horizontal tension force of a main cable was derived. To avoid to generate the additional cable tension force by sag-extensibility, the analytical solution of characteristic equation for anti-symmetrical vibration mode of the main cable was calculated. Then, the estimation of main cable tension force was carried out by anti-symmetric characteristic frequency vector. The errors of estimation due to characteristic frequency deviations were investigated through numerical analysis of the main cable of Taizhou Bridge. A field experiment was conducted to verify the proposed approach. Through measuring and analyzing the responses of a main cable of Taizhou Bridge under ambient excitation, the horizontal tension force of the main cable was identified from the first three odd frequencies. It is shown that the estimated results agree well with the designed values. The proposed approach can be used to conduct the long-term health monitoring of suspension bridges.

• Smart Structures and Systems
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• v.6 no.7
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• pp.767-792
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• 2010

In this paper, the optimal design of vibration control system for smart structures has been investigated semi-analytically via the optimization of geometric parameters like the placements and sizes of piezoelectric sensors and actuators (S/As) bonded on the structures. The criterion based on the maximization of energy dissipation was adopted for the optimization of the control system. Based on the sensing and actuating equations, the total energy stored in the system which is used as the objective function was analytically derived with design variables explicitly presented. Two cases of single and combined vibration modes were addressed for a simply supported beam and a simply supported cylindrical shell. For single vibration mode, the optimal distributions of the piezoelectric S/As could be obtained analytically. However, the Sequential Quadratic Programming (SQP) method has to be employed to solve those which violated the prescribed constraints and to solve the case of combined vibration modes. The results of three examples, which include a simply supported beam, a simply supported cylindrical shell and a simply supported plate, showed good agreement with those obtained by the Genetic Algorithm (GA) method. Moreover, in comparison with the GA method, the proposed method is more effective in obtaining better optimization results and is much more efficient in terms of computation time.

• Steel and Composite Structures
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• v.28 no.2
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• pp.167-177
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• 2018

In order to investigate mechanical properties in the core area of Square Hollow Section(SHS) column connection with external stiffening ring, four specimens were tested under the static tension load. The failure modes, load-displacement curves and strain distribution were analyzed to study the mechanical properties and the load transfer mechanism of the core area of connections. The connections behave good ductility and load-bearing capacity under the static tension load. Parametric analysis was also conducted, in which the thickness of steel tube, extended width and thickness of the stiffening ring were considered as the parameters to investigate the effects on mechanical properties of the connections. Based on the experimental results, an analytical method for the bearing capacity of connection with external stiffening ring under the static tension load was proposed. The theoretical results and the experimental results are in good agreement, which indicates that the theoretical calculation method of the bearing capacity is advisable.

• Steel and Composite Structures
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• v.34 no.1
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• pp.53-73
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• 2020

A novel all-steel miniature bar-type structural fuse (MBSF) with cold formed bolted connections is developed in this study, which consists of a central energy dissipation core cut from a smooth round bar, an external confining tube and nuts. Three types of cross sections for the central energy dissipation core, i.e., triple-cut, double-cut and single-cut cross sections, were studied. Totally 18 specimens were axially tested under either symmetric or asymmetric cyclic loading histories, where the parameters such as cut cross sectional area ratio, length of the yielding portion and cross sectional type were investigated. Numerical simulation of 2 representative specimens were also conducted. An analytical model to evaluate the bending failure at the elastic portion was proposed, and a design method to avoid this failure mode was also presented. The experimental results show that the proposed MBSFs exhibit satisfactory hysteretic performance under both the two cyclic loading histories. Average strain values of 8% and 4% are found to be respectively suitable for designing the new MBSFs as the ultimate strain under the symmetric and asymmetric cyclic loadings.