• Nutrition Research and Practice
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• v.18 no.1
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• pp.46-61
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• 2024

BACKGROUND/OBJECTIVES: An increasing life expectancy in society has burdened healthcare systems substantially because of the rising prevalence of age-related metabolic diseases. This study compared the effects of animal protein hydrolysate (APH) and casein on metabolic diseases using aged mice. MATERIALS/METHODS: Eight-week-old and 50-week-old C57BL/6J mice were used as the non-aged (YC group) and aged controls (NC group), respectively. The aged mice were divided randomly into 3 groups (NC, low-APH [LP], and high-APH [HP] and fed each experimental diet for 12 weeks. In the LP and HP groups, casein in the AIN-93G diet was substituted with 16 kcal% and 24 kcal% APH, respectively. The mice were sacrificed when they were 63-week-old, and plasma and hepatic lipid, white adipose tissue weight, hepatic glucose, lipid, and antioxidant enzyme activities, immunohistochemistry staining, and mRNA expression related to the glucose metabolism on liver and muscle were analyzed. RESULTS: Supplementation of APH in aging mice resulted in a significant decrease in visceral fat (epididymal, perirenal, retroperitoneal, and mesenteric fat) compared to the negative control (NC) group. The intraperitoneal glucose tolerance test and area under the curve analysis revealed insulin resistance in the NC group, which was alleviated by APH supplementation. APH supplementation reduced hepatic gluconeogenesis and increased glucose utilization in the liver and muscle. Furthermore, APH supplementation improved hepatic steatosis by reducing the hepatic fatty acid and phosphatidate phosphatase activity while increasing the hepatic carnitine palmitoyltransferase activity. Furthermore, in the APH supplementation groups, the red blood cell (RBC) thiobarbituric acid reactive substances and hepatic H₂O₂ levels decreased, and the RBC glutathione, hepatic catalase, and glutathione peroxidase activities increased. CONCLUSIONS: APH supplementation reduced visceral fat accumulation and alleviated obesity-related metabolic diseases, including insulin resistance and hepatic steatosis, in aged mice. Therefore, high-quality animal protein APH that reduces the molecular weight and enhances the protein digestibility-corrected amino acid score has potential as a dietary supplement for healthy aging.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.1
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• pp.39-44
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• 2002

To obtain the repression of off-odor and the improvement of food quality in b1ack bean chungkugjang (BBC), some baste components of BBC added with kiwi (BBCK) or radish (BBCR) and fermented at 42$^{\circ}C$ for 3 days were investigated. Although contents of free amino acids in BBC were lower than those of soybean chung-kugjang (SC), they increased by adding kiwi and radish homogenate to black bean, indication that two materials were effective to the enzymatic digestibility of soy protein during fermentation. Among organic acids, citric acid was the most abundant, followed by acetic acid and lactic acid. Fatty acid composition was high in the order of linoleic acid (44.28~54.24%), oleic acid (18.18~22.10%) and palmitic acid(9.93~15.51%). There was no significant difference in compositions of organic acids and fatty acids of chungkugjangs. Majar volatile compounds of BBC were 2.5-dimethyl parazine and trimethyl pyrazine. Contents of alkyl pyrazines that of contribute the characteristic aroma and flayer of BBCK and BBCR decreased as compared with those of SC, respectively. Uracil and UMP were major nucleic acid-related compounds in all four types chungkugjangs. Contents of the other nucleic acid-related compounds showed a similar trend in all chungkugjangs. In sensory evaluation, kiwi and radish were effective to repression of off-odor from chungkugjang. Sweet taste of stew of black bean chungkugjang was strong as compared with that of soybean chungkugjang, indicating that palatability of BBCK or BBCR was good.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.5
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• pp.545-555
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• 2014

To develop Korean-style cut noodles with enhanced protein and calcium levels, we manipulated the ratio of dried anchovy Engraulis japonicus soup base residue powder to wheat flour, using a response surface methodology based on trained panel trials to determine the optimum ratio. Texture analysis and nutritional evaluation were also performed on cut noodles containing dried anchovy soup base residue (CNAR). Higher umami taste and springiness, and lower fishy flavor were strongly correlated with overall acceptability. The optimal CNAR formulation consisted of wheat flour (96.02 g), anchovy residue powder (2.67 g), and water (50.64 mL). CNAR had lower gumminess and adhesiveness (P<0.001), but higher springiness, cohesiveness (P<0.001), and chewiness (P<0.05), than the control (original wheat flour cut noodles). The addition of anchovy soup base residue elevated protein, lipid, and ash levels relative to the control. Color values decreased with increasing residue powder content. In vitro protein and starch digestibility of CNAR were lower than in the control (P<0.001). CNAR yielded significantly higher total free amino acid content than the control (P<0.01), leading to CNAR's improved palatability. Dried anchovy soup base residue can be used in wheat flour cut noodles to improve nutrition, sensory acceptability, and profitability.

• Korean Journal of Plant Resources
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• v.22 no.3
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• pp.203-208
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• 2009

Lunasin is a unique 43-amino acid peptide which has shown a chemopreventive in mammalian cells and in a skin cancer mouse model. In search for new sources of lunasin and the role of cereals in cancer prevention, we report here the properties of lunasin purified from millet. Stability of millet lunasin was measured by in vitro digestibility assay using pepsin and pancreatin. Inhibition of HAT (histone acetyltransferase) and nuclear localization in mammalian cells were used to measure lunasin bioactivity as the cancer chemopreventive agent. Lunasin present in millet crude protein was stable to pepsin and pancreatin in in vitro digestion and inhibited the activities of HATs. When added exogenously, lunasin purified from millet internalized in the nuclei of mouse fibroblast cells. On the base of this result, we conclude that lunasin in millet is bioactive and consumption of millet may play an important role on cancer prevention in millet-consuming populations.

• Korean Journal of Poultry Science
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• v.10 no.1
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• pp.30-34
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• 1983

An experiment was carried out to evaluate the feeding value of hydrolyzed leather meal (HLM) replacing fish meal A total of 240 day-old female chicks of Maniker strain was divided into 20 groups of 12 birds ecah. Each groups was assigned to one of the five dietary treatments (0. 1. 2, 4 and 6% HLM). The feeding trial was conducted for six weeks. Results obtained are summarized as follows: 1. The HLM used in this experiment contained 74.41% crude protein. 17.0% crude ash and 2.0% chromium. Pepsin digestibility of the protein was measured to be 85%. Amino acid contents were also determined 2. The inclusion of HLM up to 4% in broiler rations did not depress the growth rate, feed consumption and feed efficiency of experimental birds. However, these performance parameters were significantly (p＜0.05) depressed when fed 6% HLM. These results indicate that HLM in place of fish meal can be used up to 4% in tile broiler rations.

• Food Science and Industry
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• v.55 no.2
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• pp.176-187
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• 2022

Because of human population growth, longer lifespans, and climate change, there is growing concern around world to produce enough food and feed. Insects are regarded as an alternative with high potential because the production of insects demands limited amounts of water and land, and they can add value to low-value by-products. Insects have high levels of crude protein, lipids and minerals. The relative amount of protein can vary substantially, with crude protein content ranging from 23% to 76%, depending on insect species. Their amino acid composition is good and protein digestibility is high. Insect to be a significant sustainable source as a replacement of ingredients such as soya or fishmeal in the feeds of terrestrial livestock or fish. This review provides an overview of nutritional value of insect in animal feed and challenges required to develop a sustainable, safe, and affordable insect farming industry.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.5
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• pp.733-741
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• 2007

The effects of intra-duodenal infusion of methionine (Met), lysine (Lys) and leucine (Leu) on dry matter intake (DMI), the concentrations of insulin-like growth factor I (IGF-I), growth hormone (GH) and insulin in plasma, and liver IGF-I mRNA level were investigated in two experiments for Liuyang Black growing wether goats. In Experiment 1, three goats ($10.0{\pm}0.1$ kg) were fitted with ruminal, proximal duodenal and terminal ileal fistulaes to determine the infusion amounts of Met, Lys and Leu at the duodenum according to essential amino acid flows into the duodenum and their apparent digestibility. The infusion amounts were 0.77 g/d, 0.91 g/d and 0.58 g/d respectively. In Experiment 2, 4 groups of goats (($10.0{\pm}0.2$ kg) for each group, were cannulated at the duodenum, and were infused with a mixture of Met, Lys and Leu (Control), or mixtures with 21% Met, Lys or Leu replaced with glutamate respectively on a nitrogenous basis. The replacement of 21% Met, Lys or Leu with glutamate did not affect intakes of maize stover, concentrate or both (p>0.05) when compared with the control. The replacement of 21% Met or Lys significantly (p<0.05) reduced plasma GH, insulin and IGF-I concentrations and liver IGF-I mRNA level. The replacement of 21% Leu with glutamate reduced (p<0.05) plasma IGF-I concentration only, but not plasma insulin and GH, as well as liver IGF-I mRNA level (p>0.05). The close relationships between supplying Met and Lys in the lumen of the duodenum and plasma IGF-I, GH and insulin concentrations, as well as liver IGF-I mRNA level in this study indicate that the effects of the limiting amino acids on nutrition of animals are likely intermediated via their effects on these hormones, and these hormone profiles could be used as intermediate markers for the limiting order of amino acids.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.9
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• pp.1159-1165
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• 2010

Glucose is the main energy source for mammalian cells and its absorption is co-mediated by two different families of glucose transporters, sodium/glucose co-transporters (SGLTs) and facilitative glucose transporters (GLUTs). Here, we report the cloning and tissue distribution of porcine GLUT2. The GLUT2 was cloned by RACE and its cDNA was 2,051 bp long (GenBank accession no. EF140874). An AAATAA consensus sequence at nucleotide positions 1936-1941 was located upstream of the poly $(A)^+$ tail. Open reading frame analysis suggested that porcine GLUT2 contained 524 amino acids, with molecular weight of 57 kDa. The amino acid sequence of porcine GLUT2 was 87% and 79.4% identical with human and mouse GLUT2, respectively. GLUT2 mRNA was detected at highest level in porcine liver, at moderate levels in the small intestine and kidney, and at low levels in the brain, lung, muscle and heart. In the small intestine, the highest level was in the jejunum. In conclusion, the mRNA expression of GLUT2 was not only differentially regulated by age, but also differentially distributed along the small intestine of piglets, which may be related to availability of different intestinal luminal substrate concentrations resulting from different food sources and digestibility.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.2
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• pp.305-327
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• 1999

In the paper insight is given in the legislation policy to restrain environmental pollution by pig husbandry, focused on The Netherlands (Mineral Accounting System). Besides, nutritional measures are presented to reduce environmental pollution by lowering excretion of N and P, emphasizing (multi) phase feeding, the use of low protein, synthetic amino acids supplemented diets, phytase and its effect on phosphorus and calcium digestibility, its interaction with phytic acid and proteins, and the environmental impact of the use of phytase in pig diets. Also, nutritional means are indicated to reduce ammonia volatilization from pig operations. It is concluded that nutrition management can substantially contribute to reduction of N and P excretion by pigs, mainly by lowering dietary protein levels, (multi) phase feeding and the use of microbial phytase, and that the use of phytase on a large scale in The Netherlands has a tremendous environmental impact. In 20 years the excretion of P in growing-finishing pigs has more than halved. Ammonia emission from manure of pigs can be reduced substantially by lowering dietary protein content, but also by including additional non-starch polysaccharides in the diet. A very promising method to reduce ammonia emission is to manipulate dietary cation-anion difference, e.g. by adding acidifying salts to the diet, which will lower pH of urine substantially. Further research is desirable. This also applies to determining dietary factors influencing the odour release from manure. Finally, some speculation on the future of pig farming from an environmental viewpoint is presented.

• Korean Journal of Food Science and Technology
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• v.12 no.2
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• pp.109-114
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• 1980

Protein efficiency ratio (PER), net protein utilization (NPU), nitrogen digestibility (ND), organ weights, and amino acid composition were investigated for rapeseed protein isolates (RPI) prepared by alkaline countercurrent extraction and isoelectric precipitation of defatted rapeseed meal (DRM). The PER values for 3 kinds of RPI obtained at pI of 6.7, 5.6 and 5.0 were 2.8, 3.1 and 2.9, and for DRM 2.5 while the NPU values for these RPI appeared to be 68, 73 and 71 %, and for DRM 56 %, respectively. Mean ND (85%), food intake (87), and weight gain (37.6) for RPI were significantly different from those of DRM (71 %, 77, and 28, respectively). There was no signicant difference in the PER among three RPI and casein (3.0), nor in the NPU among those proteins and casein (74%). These data attributed to the favorable amino acid patterns of proteins isolated which contained balanced essential amino acids in proportions which meet the requirements of human adult(FAO/WHO, 1973).