• Journal of the Korean Society of Groundwater Environment
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• v.6 no.4
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• pp.194-205
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• 1999

Hydrogeochemical variation and environmental isotope at the some abandoned metal mine (Sanggok, Keumsil, Jangpung and Samdeok) creeks of the Hwanggangri mining district were carried out based upon the physicochemical properties for surface water collected of February in 1998. Hydrogeochemical composition of the all water samples are characterized by the relatively significant enrichment of Ca$^{2}$, alkaline ions, N $O_3$$^{－}$ and Cl$^{－}$ in normal surface water, whereas the surface waters near the mining area are relatively enriched in Ca$^{2+$, Mg$^{2＋}$, heavy metals. HC $O_3$$^{－}$ and S $O_4$$^{2－}$. Surface waters of the mining creek have low pH, high EC and extremely high concentrations of TDS compared with surface water of the non-mining creeks. The range of $\delta$D and $\delta$$^{18}$O values (SMOW) in the waters are shown in －65.0 to－71.2$\textperthousand$ and －9.1 to－10.2$\textperthousand$. The d($\delta$D－$\delta$$^{18}$O) value with those of water samples ranged from 7.3 to 10.9. These $\delta$D and $\delta$$^{18/}$ of the acid mine water are more heavy values than those of surface water. The values have revealed the positive correlation between isotopic compositions and major elements, because those $\delta$D and $\delta$$^{18}$O values increase with increasing TDS. HC $O_3$$^{－}$ , S $O_4$$^{2－}$ and Ca$^{2＋}$ concentration. Using WATEQ4F, saturation index of albite calcite, dolomite and mostly clay minerals in water of the mining area show undersaturated and progressively evolved toward the equilibrium condition due to fresh water mixing, however, surface waters of the non-mining area are nearly saturated and/or supersaturated. Geochemical modeling showed that mostly toxic heavy metals within water in the mining creek may exist largely in the from of metal-sulfate (MS $O_4$$^{2－}$), free metal (M$^{2＋}$/), C $O_3$$^{－}$ and/or OH$^{－}$ complex ions. Based on the geology, water chemistry and environmental istopic data the water compositions from the Sanggok and Keumsil mine creek (consist mainly of Cambro-Ordovician carbonate rocks of the Cho-seon Supergroup) show higher PH, Ca$^{2＋}$, Mg$^{2＋}$ , HC $O_3$$^{－}$ and more heavy $\delta$D and $\delta$$^{18}$O values than those from the Jangpung and Samdeok mine creek (consist of age -unknown metasedimentary rocks of the Ogcheon Supergroup and/or Jurassic grani-toids), but each of these waters represents a similar hydrogeochemical evolution path by the mine water mixing.

• Korean Journal of Soil Science and Fertilizer
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• v.38 no.1
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• pp.15-24
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• 2005

Enterobacter intermedium oxidizes glucose to gluconic acid and sequentially converts gluconic acid to 2-ketogluconic acid (2-KGA) under aerobic condition. Shaking incubation of E. intermedium in a broth medium containing 22.5 g glucose, 8.2 g gluconic acid and 40 g rock phosphate per liter resulted in $1028mg\;L^{-1}$ soluble phosphate. The culture broth was used as phosphate bio-fertilizer (PBF) in this experiment. To evaluate PBF produced by E. intermedium on lettuce growth, five treatments (PBF1/3, PBF2/3, PBF3/3, BP, and MF) were used. In MF and BP treatments, $P_2O_5$ 5.9 kg of mineral fertilizer per 10a was added, while in PBF1/3, PBF2/3, and PBF3/3 treatments, culture broth containing one third, two third, and same amount of soluble $P_2O_5$ 5.9 kg per 10a was applied, respectively. At 20, 35, and 50 days after transplanting of lettuce, plant growth components, biomass, enzyme activities and soil chemical properties were analyzed. Dehydrogenase activity and available phosphate concentration of rhizosphere in phosphate bio fertilizer treatments (PBF1/3, PBF2/3, PBF3/3) were generally higher compared to MF and BP treatments. Soil biomass in PBF3/3 treatment was significantly higher than MF and BP treatments at early growth stage. However, there was no significant difference among all treatments with time. Plant fresh weights in PBF1/3, PBF2/3, and MF treatments were better than those in BP and PBF3/3 treatments. However, in PBF2/3 treatment the highest fresh weight was discovered where alkaline phosphatase activity was generally higher than other treatments at 35 and 50 days. Enhancement of lettuce growth at 35 and 50 days in PBF2/3 treatment was associated with greater phosphate uptake in lettuce tissue. As regarding all results, PBF showed better lettuce growth compared to mineral phosphate fertilizer where PBF2/3 treatment resulted in increase of lettuce fresh weight by 23% and phosphate uptake by 50%.

• Korean Journal of Environmental Biology
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• v.24 no.2 s.62
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• pp.102-111
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• 2006

The increased occurrence of hyperglycemia and oxidative stress in streptozotocin (STZ) induced type I diabetes has been implicated in the etiology and pathology of disease complication. STZ has known to be genotoxic in a variety of assays including tests for microbial mutagenesis and unscheduled DNA synthesis in rat kidney. Diabetes mellitus (DM) is a pathologic condition, resulting in severe metabolic imbalances and non-physiologic changes in many tissues. We examined the effect of gamma radiation and KWNP on preventing the development of insulin dependent diabetes mellitus using streptozotocin-induced Fisher 344 diabetic rats. The hematological values (red blood cell and white blood cell), serum biochemical constituents-alkaline phosphatase (ALP), total cholesterol, triglycerides and insulin-were checked and the organs (testis, spleen and kidney) were weighed. The gonad indices of the STZ treated groups were much lower than the value of the control group. But the gonad indices of the KWNP treated groups were higher than those of the treated groups. The ratio of the weight of kidney to the body weight of the STZ treated groups was higher than that of the control group. The value of the diabetic group treated with KWNP after irradiation (F group) was lower than the other STZ treated groups. The white blood cell and ALP values of the F group were lower than the other STZ groups, as well. The cholesterol and triglyceride values of all the KWNP treated groups were significantly lower than the other groups. A significant increase (about 10 times) of insulin was detected in the F group. The results of hematological assay showed the distinctive damage in the irradiated and STZ treated groups. The quantity of apoptotic cells in seminiferous tubule of testis confirmed a serious damage as assessed in the STZ treated groups. These experimental results have revealed that treatment of the products of KWNP after irradiation has the antidiabetic effect in the STZ-induced diabetic rats. But the F group showed higher recuperative power. These experimental results have revealed that treatment of the gamma irradiation and KWNP have the recovering effect in the STZ-induced diabetic rats.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.3
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• pp.184-190
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• 1984

Sardine, Sardinops melanosticta, has been caught more than fifty thousand metric tons every year in adjacent sea of Korea, but most of them used for uneatable fish meal because of their rapid spoilage. Usually it is known that the main reason of putrefaction of foods is caused by the maicro-organisms included in them. Therefore, this experiment was carried out to identify the micro-organisms isolated from the intestine of fresh sardine and characterize their proteolytic enzymes produced from them. Aerobic cell count ranged from $1.7{\times}10^4\;to\;3.6{\times}10^5/g$, while anaerobic cell count, from $2.9{\times}10^4\;to\;5.5{\times}10^5/g$. Most of the isolated strains were psychrotrophic mesophiles. Among the two hundred and eighty strains isolated from the fresh samples, fifty-six strains ($20.0\%$) were proteolytics, one hundred and seventy-five strains ($62.5\%$) were lipolytics and tenty-nine strains ($10.5\%$) had the ability to produce hydrogn sulfide. The most predominantly isolated microbial groups from the fresh sardine were Moraxella ($31.4\%$) and Pseudomonas sup. ($28.6\%$). Flavobacterium-Cytophaga, Vibrio, Acinetobacter, Micrococcus spp. and Enterobacteriaceae appeared from $7.9\%\;to\;5\%$ out of total tested strains. The average bacterial count in the spoiled samples (stored at about $18^{\circ}C$ for 48 hours) was increased to the level of $2.9{\times}10^8/g$ for aerobes, $1.5{\times}10^8/g$ for anaerobes, then one hundred and ten strains, corresponding to $52\%$, out of two hundred and thirteen strains submitted to the test were proteolytics. The strongest proteolytic bacterium among the two hundred and eighty strains was identified as Pseudomonas 101 which grew best at $25^{\circ}C$. The optimum condition for the activity of the proteolytic enzyme produced by Pseudomonas 101 appeared $35^{\circ}C$ and pH 9.0, but the activity was relatively unchanged between 5.0 and 11.0 of pH and between $30^{\circ}C\;and\;50^{\circ}C$ of temperature.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.31 no.4
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• pp.281-286
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• 2009

Introduction: Enamel matrix derivative (EMD) is a protein which is secreted by Hertwig root sheath and plays a major role in the formation of cementum and attachment of peridontium. Several studies have shown that EMD promoted the proliferation and differentiation of preosteoblasts, osteoblasts and periodontal ligament cells in vitro: however, reports showing the inhibition of osteogenic differentiation by EMD also existed. This study was designed to simultaneously evaluate the effect of EMD on the two cell lines (human mesenchymal stem cells: hMSC, human periodontal ligament derived fibroblasts: hPDLCs) by means of quantitative analysis of some bone related matrices (Alkaline phosphatase : ALP, osteopontin ; OPN, osteocalcin ; OC). Materials and Methods: hMSCs and hPDLCs were expanded and cells in the 4${\sim}$6 passages were adopted to use. hMSc and hPDLCs were cultured during 1,2,7, and 14 days with 0, 50 and 100 ${\mu}g/ml$ of EMD, respectively. ALP activity was assessed by SensoLyte ALP kit and expressed as values of the relative optical density. Among the matrix proteins of the bony tissue, OC and OPN were assessed and quantification of these proteins was evaluated by means of human OC immunoassay kit and human OPN assay kit, respectively. Results: ALP activity maintained without EMD at $1,2^{nd}$ day. The activity increased at $7^{th}$ day but decreased at $14^{th}$ day. EMD increased the activity at $14^{th}$ day in the hPDLCs culture. In the hMSCs, rapid decrease was noted in $7^{th}$ and $14^{th}$ days without regard to EMD concentrations. Regarding the OPN synthesis in hPDLCs, marked decrease of OPN was noted after EMD application. Gradual decrease tendency of OPN was shown over time. In hMSCs, marked decrease of OPN was also noted after EMD application. Overall concentration of OPN was relatively consistent over time than that in hPDLCs. Regarding the OC synthesis, in both of hPDLCs and hMSCs, inhibition of OC formation was noted after EMD application in the early stages but EMD exerted minimal effect at the later stages. Conclusion: In this experimental condition, EMD seemed to play an inhibitory role during the differentiation of hMSCs and hPDLCs in the context of OC and OPN formation. In the periodontium, there are many kinds of cells contributing to the regeneration of oral tissue. EMD enhanced ALP activity in hPDLCs rather than in hMSCs and this may imply that EMD has a positive effect on the differentiation of cementoblasts compared with the effect on hMSCs. The result of our research was consistent with recent studies in which the authors showed the inhibitory effect of EMD in terms of the differentiation of mineral colony forming cells in vitro. This in vitro study may not stand for all the charateristics of EMD; thus, further studies involving many other bone matrices and cellular attachment will be necessary.

• Journal of the Korean Society of Groundwater Environment
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• v.6 no.2
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• pp.59-65
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• 1999

Both the groundwater changes due to different pumping rates and the geochemistry of thermal waters in the Suanbo area are considered in this study. The observation of groundwater level change since 1991 shows that the change is directly correlated with pumping rates of thermal waters and reveals the retardation of ca. 5 weeks after pumping. The hydrogeological aquifer in the area is under reducing condition. The thermal waters are of Na-HCO$_3$ type. and are alkaline (pH=8.5∼8.7) with low TDS values (274∼284 mg/l) and high concentrations of Na (68∼72 mg/l). F (6.4∼8.9 mg/l), and HCO$_3$(136∼146 mg/l). Oxygen and hydrogen isotope ratios of thermal water indicate a meteoric water origin. The activities of Rn-222 and Ra-226 in both thermal water and local groundwater were determined to delineate possible geochemical controls on the Rn-222 and Ra-226. The Rn-222 concentrations are several orders of magnitude greater than the Ra-226 concentrations. The concentrations of Rn-222 range from 190 to 7.490 pCi/1 with an average of 2,522 pCil/l. and those of Ra-226 average 0.32 pCi/1 with the range from 0.25 to 0.42 pCi/1. The concentrations of Rn-222 and Ra-226 are inversely correlated with EC and alkalinity. The pH it positively correlated with Ra-226. The correlation between Rn-222 and Ra-226 is poor. Thermal waters in the study area are produced from highly fractured phyllite. The thermal water qualify. CSAMT (controled-source audiofrequency magnetotelluric) prospecting, and petrological evidences, however, indicate that the heat is possibly transmitted through deep normal faults reaching a deep granite batholith, and the phyllite acts only as a groundwater pathway.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.1
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• pp.76-82
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• 2009

Wheat is the most widely cultivated cereal and an important source of dietary protein worldwide. Wheat allergy, defined as an adverse immunologic reaction to wheat, encompasses a broad spectrum of disorders with different pathomechanisms and clinical manifestation. The Nuruk, a traditional Korean Koji for brewing, was made with wheat flour and fermenting microbes such as bacteria, yeast and mold. The strains grown on Nuruk secrete various enzymes as amylase and protease. By the activation of such enzymes, starch and proteins in Nuruk are hydrolyzed to sugar and amino acid. Therefore, it is supposed to reduce allergic proteins in wheat. To study quality properties and degradation degree of allergenicity in Nuruk by fermentation, we investigated the changes of general ingredients and allergenicity in Nuruk during fermentation. Moisture contents was decreased from 24.2% to 13.6% during fermentation. Crude lipid and protein contents were gradually increased during fermentation. After 15 days of fermentation, reducing sugar and total sugar contents were reached its maximum level, and they were 27.45% and 39.00%, respectively. Acid and neutral protease activity were significantly increased during fermentation, but alkaline protease activity was not detected. ${\alpha}$-amylase activity was gradually increased and showed maximum level about 2,833.00 U/g after 15 days of fermentation. Glucoamylase activity was the highest level about 497.9 U/g after 10 days of fermentation. The increase of these proteolytic and saccharogenic enzyme activities will provide efficient condition for production of rice wine. Also, protein fractions were isolated from Nuruk, and degradation of these proteins during fermentation were confirmed by SDS-PAGE. IgE immunoblotting using patient's sera with wheat allergy was performed to confirm allergenic protein in Nuruk. These results as fermentation of Nuruk will provide a useful tool for developing safer wheat products to prevent wheat allergy.

• Journal of the Mineralogical Society of Korea
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• v.24 no.2
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• pp.73-82
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• 2011

X-ray absorption spectroscopy (XAS) study was conducted using arsenite-sorbed two-line ferrihydrite to investigate the mechanism of surface interactions between two-line ferrihydrite and As(III) (arsenite) which are ubiquitous in nature. The two-line ferrihydrite used was synthesized in the laboratory and the study was undertaken at pHs 4 and 10 to compare the difference in mechanisms of surface interaction between acidic and alkaline environments. The effect of arsenite-adsorbed concentrations on surface complexation was investigated at each pH condition as well. From the results of XAS analyses, the structural parameters of arsenite in the EXAFS revealed that the coordination number and distanceof As-O were 3.1~3.3 and 1.74~1.79 ${\AA}$, respectively, which indicate that the unit structure of arsenite complex formed on the surface of two-line ferrihydrite is $AsO_3$. The dominant structures of As(III)-Fe complex were examined to be bidentate binuclear comer-sharing ($^2C$) and the mixture of bidentate mononuclear edge sharing ($^2E$) and $^2C$ appeared as well. At pH 4, arsenite complex showed different structures on the surface of two-line ferrihydrite, depending on the adsorbed concentrations. At pH 10, on the contrary, the surface structures of arsenite complexes were interpreted to be almost identical, irrespective of the adsorbed concentrations of arsenite. Consequently, this microscopic XAS results support the results of macroscopic adsorption experiments in which the surface interaction between arsenite and two-line ferrihydrite is significantly influenced by pH conditions as well as arsenite concentrations.

• Journal of the Mineralogical Society of Korea
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• v.22 no.3
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• pp.177-189
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• 2009

Iron (oxyhydr)oxides commonly form as secondary minerals of high reactivity and large surface area resulting from alteration and weathering of primary minerals, and they are efficient sorbents for inorganic and organic contaminants. Accordingly, they have a great potential in industrial applications and are also of substantial interest in environmental sciences. Goethite (${\alpha}$-FeOOH) is one of the most ubiquitous and stable forms of iron (oxyhydr)oxides in terrestrial soils, sediments, and ore deposits, as well as a common weathering product in rocks of all types. This study focused on adsorption reaction as a main mechanism in scavenging arsenic using goethite. Goethite was synthesized in the laboratory to get high purity, and a variety of mineralogical and physicochemical features of goethite were measured and related to adsorption characteristics of arsenic. To compare differences in adsorption reactions between arsenic species, in addition, a variety of experiments to acquire adsorption isotherm, adsorption edges, and adsorption kinetics were accomplished. The point of zero charge (PZC) of the laboratory-synthesized goethite was measured to be 7.6, which value seems to be relatively higher, compared to those of other iron (oxyhydr)oxides. Its specific surface area appeared to be $29.2\;m^2/g$ and it is relatively smaller than those of other (oxyhydr)oxides. As a result, it was speculated that goethite shows a smaller adsorption capacity. It is likely that the affinity of goethite is much more larger for As(III) (arsenite) than for As(V) (arsenate), because As(III) was observed to be much more adsorbed on goethite than As(V) in equivalent pH conditions. When the adsorption of each arsenic species onto goethite was characterized in various of pH, the adsorption of As(III) was largest in neutral pH range (7.0~9.0) and decreased in both acidic and alkaline pH conditions. In the case of As(V), the adsorption appeared to be highest in the lowest pH condition, and then decreased with an increase of pH. This peculiarity of arsenic adsorption onto goethite might be caused by macroscopic electrostatic interactions due to variation in chemical speciation of arsenic and surface charge of goethite, and also it is significantly affected by change in pH. Parabolic diffusion model was adequate to effectively evaluate arsenic adsorption on goethite, and the regression results show that the kinetic constant of As(V) is larger than that of As(III).

• Korean Journal of Food Science and Technology
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• v.9 no.3
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• pp.211-219
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• 1977

Dehulled and defatted Korean cottonseed flour was extracted with alkaline solution for 30 minutes and had precipitated the crude portein by adjusting pH $1{\sim}12$. The general composition and the amino acid composition of cottonseed protein were analyzed. Crude protein was purified with sephadex G-100 and G-200, and its component had been identified by disc electrophoresis. Toxic gossypol was removed by n-hexane, acetone and other solvents. The results were as follows. (1) pH 5, pH 7 and pH 4 were the best condition of precipitation of curde protein at single, two step and water extraction, respectively. (2) The cottonseed flour which was dehulled and defatted, contained 61.3% of crude protein. (3) The protein which was isolated from cottonseed flour, contained 20% of glutamic acid, and comparatively high levels of essential amino acids. (4) Dehulled cottonseed flour contained 0.97% of total gossypol and could be romoved 70% of total gossypol by extraction with n-hexane. (5) 10-13 bands of water soluble protein were found in disc electrophoresis, and 10-12 bands in protein were isolated by single and two step procedures. (6) The cottonseed protein could be purified by sephadex G-100 and G-200. (7) 10-20% of gossypol-free cottonseed fluor could be used for animal and human comsumption.