• KIPS Transactions on Software and Data Engineering
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• v.5 no.1
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• pp.35-42
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• 2016

The core of the machine vision that is frequently used at the pre/post-production stages is a marker alignment technology. In this paper, a method to detect the angle and position of a product at high speed by use of a unique pattern present in the marker stamped on the product, and calibrate them is proposed. In the proposed method, to determine the angle and position of a marker, the candidates of the marker are extracted by using a variation of the integral histogram, and then clustering is applied to reduce the candidates. The experimental results revealed about 5s 719ms improvement in processing time and better precision in detecting the rotation angle of a product.

• Applied Microscopy
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• v.43 no.1
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• pp.1-8
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• 2013

We developed an improved method for tilt series alignment with fiducial markers in electron tomography. Based on previous works regarding alignment, we adapted the Levenberg-Marquardt method to solve the nonlinear least squares problem by incorporating a new formula for the alignment model. We also suggested a new method to estimate the initial value for inversion with higher accuracy. The proposed approach was applied to geopolymers. A better alignment of the tilt series was achieved than that by IMOD S/W. The initial value estimation provided both stability and a good rate of convergence since the new method uses all marker positions, including those partly covering the tilt images.

• Current Optics and Photonics
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• v.8 no.1
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• pp.72-79
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• 2024

Recent flat-panel displays have become increasingly complicated to facilitate multiple display functions. In particular, the form of multilayered architectures for next-generation displays makes precise three-dimensional alignment of multiple panels a challenge. In this paper, a diffractive optical alignment marker is proposed to address the problem of three-dimensional alignment of distant dual panels beyond the depth-of-focus of a vision camera. The diffractive marker is effective to analyze the positional correlation of distant dual panels. The possibility of diffractive alignment in multilayer display fabrication is testified with numerical simulation and a proof-of-concept experiment.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.274.1-274.1
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• 2013

We report the circumferential alignment of human aortic smooth muscle cells (HASMCs) in an orthogonally micropatterned circular microfluidic channel to form an in vivo-like smooth muscle cell layer. To realize a biomimetic smooth muscle cell layer which is aligned perpendicular to the axis of blood vessel, we first fabricated a half-circular polydimethylsiloxane (PDMS) microchannel by soft lithography using a convex PDMS mold. The orthogonally micro wrinkle patterns were generated inside the half-circular microchannel by stretching-releasing operation under UV irradiation. Upon UV treatment with uniaxial 40 % stretch of a PDMS substrate and releasing process, the microwrinkle patterns perpendicular to the axial direction of the circular microchannel were generated, which could guide the circumferential alignment of HASMCs successfully during cultivation. The analysis of orientation angle, shape index, and contractile protein marker expression indicates that the cultured HASMCs revealed the in vivo-like cell phenotype. Finally, we produced circular microchannels by bonding two half-circular microchannels, and cultured the HASMCs circumferentially with high alignment and viability for 5 days. These results are the first demonstration for constructing an in vivo-like 3D smooth muscle cell layer in the circular microfluidic channel which can provide novel bioassay platforms for in-depth study of HASMC biology and vascular function.

• Korean Journal of Applied Biomechanics
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• v.22 no.1
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• pp.123-129
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• 2012

Simple camera-based system for evaluation of lower limb alignment as a part of an automated cycling fitting system was developed and verified in this study. Developed imaging system can evaluate lower limb alignment quantitatively during pedaling using a general camcorder and single marker attached on the knee. Threshold-based marker detection algorithm was proposed in this study. Experiment was carried out to compare the trajectory data from marker detection algorithm of the developed imaging system with the trajectory data from 3-D motion capture system. Results showed that average error between trajectories was 2.33 mm (0.92 %) in the vertical direction and 0.62 mm (1.86 %) in the medio-lateral direction. There existed significant correlation between two measured values (r=0.9996 in the vertical direction and r=0.9975 in the medio-lateral direction). It can be concluded that developed imaging system be applied to evaluate lower limb alignment which is an important factor for dynamic bicycle fitting.

• Proceedings of the IEEK Conference
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• 2007.07a
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• pp.207-208
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• 2007

In this paper, the tilted image sequence, which is obtained the transmission electron microscopy (TEM) for a 3D reconstruction, is aligned based on the fiducial marker method. A direct correlation method is also conducted between adjacent tilted images for the performance comparison. Using real TEM tilted images, we can successfully perform the alignment.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.7 no.2
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• pp.100-109
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• 2008

Traffic accidents at night occur more than any other time because of improper road light facility and delineators. Therefore, cost-effective criteria of installing delineators are needed instead of expensive road light facility, especially, on rural road including light volume of traffic. This paper presents the criteria of installing 'Chevron Alignment Sign' considering driver's visual behavior characteristics and 'Raised Pavement Marker' considering critical encroachment angie of both straight section and curve one in order to reduce both the number of accidents on curve sections and the number of road encroachment accidents, respectively. The characteristics of visual behaviors can be expressed by visual angle involving curve radius and intersection angle. The estimated installing angles are $1^{\circ}{\sim}2.5^{\circ}$ by radii, which is based on changes in sensitivity across visual field by exogenous attention. Also, the raised pavement marker is installed every 2m, 3m, and 4m considering critical encroachment angles by radii.

• The Journal of Korea Robotics Society
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• v.15 no.4
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• pp.350-356
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• 2020

Workers have been replaced by mobile manipulators for factory automation in recent years. One of the typical tasks for automation is that a mobile manipulator moves to a target location and picks and places an object on the worktable. However, due to the pose estimation error of the mobile platform, the robot cannot reach the exact target position, which prevents the manipulator from being able to accurately pick and place the object on the worktable. In this study, we developed an automatic alignment system using a low-cost camera mounted on the end-effector of a collaborative robot. Camera calibration and pose estimation methods were also proposed for the automatic alignment system. This algorithm uses a markerboard composed of markers to calibrate the camera and then precisely estimate the camera pose. Experimental results demonstrate that the mobile manipulator can perform successful pick and place tasks on various conditions.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.10
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• pp.1395-1397
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• 2002

The tyrosinase gene was selected as a candidate for uncovering genetic mechanism causing 'black ear' coat color in rabbits. A PCR-SSCP detection method was established for the $881^A{\rightarrow}881^G$ mutation located in the central region of the tyrosinase gene between the CuA and CuB binding region signatures, and this was confirmed by sequencing and alignment. Fully consistent associations between the SNP and 'black ear' coat color were observed by analysis in a "black ear" pedigree and on 61 unrelated individuals. This SNP can serve as a molecular marker for use in "back ear" wool rabbit breeding.

• The Korean Journal of Mycology
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• v.39 no.1
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• pp.22-30
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• 2011

Weonhyeong is one of important commercial strains. It has good characteristics of bundle formation, grey colored pilei and high productivity. We previously reported grouping of 70 strains of Pleurotus ostreatus in which one group contained 35 strains including Weonhyeong. Four strains in that group showed same profiles implicating no variety distinction for mushroom cultivation. Now we developed a specific marker for identification of Weonhyeong. Sequence Characterized Amplified Region (SCAR) marker was developed from the RAPD amplicon. SCAR marker 'S-OPO5' produced only one band specific to 2183, 2240, 2595 and 2725 strains showing similar banding patterns to Weonhyeong in RAPD-PCR results. The sequence of 'S-OPO5' marker was unknown when compared with the data in the Genbank using BLASTN. BLASTX results indicated that the marker showed significant alignment with the protein sequences in Tricholoma bakamatsutake reverse transcriptase. The results indicate that this new SCAR marker ('S-OPO5') will be valuable to distinguish the Weonhyeong similar strains from Pleurotus spp.