• The Plant Pathology Journal
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• 제39권2호
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• pp.207-219
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• 2023

Soft rot is a widespread, catastrophic disease caused by Pectobacterium carotovorum subsp. carotovorum (Pcc) that severely damages the production of Amorphophallus spp. This study evaluated the rhizosphere bacterial and fungal communities in Pcc-infected and uninfected plants of two species of Amorphophallus, A. muelleri and A. konjac. Principal component analysis showed that the samples formed different clusters according to the Pcc infection status, indicating that Pcc infection can cause a large number of changes in the bacterial and fungal communities in the Amorphophallus spp. rhizosphere soil. However, the response mechanisms of A. muelleri and A. konjac are different. There was little difference in the overall microbial species composition among the four treatments, but the relative abundances of core microbiome members were significantly different. The relative abundances of Actinobacteria, Chloroflexi, Acidobacteria, Firmicutes, Bacillus, and Lysobacter were lower in infected A. konjac plants than in healthy plants; in contrast, those of infected A. muelleri plants were higher than those in healthy plants. For fungi, the relative abundances of Ascomycota and Fusarium in the rhizosphere of infected A. konjac plants were significantly higher than those of healthy plants, but those of infected A. muelleri plants were lower than those of healthy plants. The relative abundance of beneficial Penicillium fungi was lower in infected A. konjac plants than in healthy plants, and that of infected A. muelleri plants was higher than that of healthy plants. These findings can provide theoretical references for further functional research and utilization of Amorphophallus spp. rhizosphere microbial communities in the future.

• Journal of Microbiology and Biotechnology
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• 제24권8호
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• pp.1088-1095
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• 2014

A systematic investigation was performed on the bacterial, Bacillus, fungal, and yeast communities of the three types of Daqu (mechanically prepared, manually prepared, and mixed prepared) used in Baiyunbian Company by reconditioning PCR-denaturing gradient gel electrophoresis (PCR-DGGE). The DGGE results showed that the microbes in the three types of Daqu were mainly thermotolerant and thermophilic microbes, and the most dominant bacterial species were Bacillus and Virgibacillus, followed by Lactobacillus and Trichococcus. Furthermore, the dominant fungi were found to be molds, such as Rasamsonia, Penicillium, Aspergillus, and Monascus, and the dominant yeasts were Saccharomyces cerevisiae, Saccharomycopsis fibuligera, Pichia anomala, and Debaryomyces hansenii. In general, the three types of Daqu showed slight differences in microbial communities, and the Shannon indexes (H') of the manually prepared and mechanically prepared Daqu were similar. The results suggest that mechanically prepared Daqu can replace manually prepared Daqu in liquor production, and this research provides useful information for liquor production and process improvement.

• Journal of Microbiology and Biotechnology
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• 제33권8호
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• pp.1013-1022
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• 2023

Arbuscular mycorrhizal fungi (AMF) are widespread soil endophytic fungi, forming mutualistic relationships with the vast majority of land plants. Biochar (BC) has been reported to improve soil fertility and promote plant growth. However, limited studies are available concerning the combined effects of AMF and BC on soil community structure and plant growth. In this work, a pot experiment was designed to investigate the effects of AMF and BC on the rhizosphere microbial community of Allium fistulosum L. Using Illumina high-throughput sequencing, we showed that inoculation of AMF and BC had a significant impact on soil microbial community composition, diversity, and versatility. Increases were observed in both plant growth (the plant height by 8.6%, shoot fresh weight by 12.1%) and root morphological traits (average diameter by 20.5%). The phylogenetic tree also showed differences in the fungal community composition in A. fistulosum. In addition, Linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed that 16 biomarkers were detected in the control (CK) and AMF treatment, while only 3 were detected in the AMF + BC treatment. Molecular ecological network analysis showed that the AMF + BC treatment group had a more complex network of fungal communities, as evidenced by higher average connectivity. The functional composition spectrum showed significant differences in the functional distribution of soil microbial communities among different fungal genera. The structural equation model (SEM) confirmed that AMF could improve the microbial multifunctionality by regulating the rhizosphere fungal diversity and soil properties. Our findings provide new information on the effects of AMF and biochar on plants and soil microbial communities.

• 농촌계획
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• 제25권3호
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• pp.103-114
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• 2019

The purpose of this study is to investigate the value perception changes of early adolescents, who are from 5th grades to 7th grades regarding to agriculture and rural communities through debate education. Since current education policy to educate agricultural value is limited in solving stereotypes and acquiring abundant relevant knowledge about agricultural values, this study tried to test the effect of debate education for the change of value perception of early adolescents about agriculture and rural communities. Through the two weeks of a debate education course, the differences of perception changes between the experimental group and control group were analyzed. As a result, education on agriculture and rural communities through debate has a great influence on the change of value perception and thus, it could be used as a supplementary educational method for literacy education and field trip learning. Lastly, limitations and implications for the study is discussed.

• 가정과삶의질연구
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• 제23권6호
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• pp.145-153
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• 2005

The purpose of this study was to analyze the impacts of consumers' demographic characteristics on their involvement in retired communities, under the assumption that consumers' involvement in retired communities would be a preceding factor of the consumers' preferences for retired communities. One of the major findings of this study was that there were statistically significant differences in the involvement in retired communities by several demographic variables such as age, gender, level of education, income, total family asset, consumer values, and pursuit of happiness. Second, such involvement was strongly correlated with consumer preferences. Third, the community management styles, such as sales and rental conditions, significantly influenced consumers' level of preference. The results from this study could provide useful information not only for understanding the residents of retired communities, buffer enhancing the welfare of residents of retired communities.

• Mycobiology
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• 제47권2호
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• pp.180-190
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• 2019

In this study, eight-month-old ectomycorrhizae of Tuber borchii with Corylus avellana were synthesized to explore the influence of T. borchii colonization on the soil properties and the microbial communities associated with C. avellana during the early symbiotic stage. The results showed that the bacterial richness and diversity in the ectomycorrhizae were significantly higher than those in the control roots, whereas the fungal diversity was not changed in response to T. borchii colonization. Tuber was the dominant taxon (82.97%) in ectomycorrhizae. Some pathogenic fungi, including Ilyonectria and Podospora, and other competitive mycorrhizal fungi, such as Hymenochaete, had significantly lower abundance in the T. borchii inoculation treatment. It was found that the ectomycorrhizae of C. avellana contained some more abundant bacterial genera (e.g., Rhizobium, Pedomicrobium, Ilumatobacter, Streptomyces, and Geobacillus) and fungal genera (e.g., Trechispora and Humicola) than the control roots. The properties of rhizosphere soils were also changed by T. borchii colonization, like available nitrogen, available phosphorus and exchangeable magnesium, which indicated a feedback effect of mycorrhizal synthesis on soil properties. Overall, this work highlighted the interactions between the symbionts and the microbes present in the host, which shed light on our understanding of the ecological functions of T. borchii and facilitate its commercial cultivation.

• 한국환경농학회지
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• 제28권4호
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• pp.365-370
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• 2009

The aim of this study was to investigate the vegetation types of Minarimot, in Jeju Islands. The vegetation types were classified by the Z-M school method and cluster analysis. The vegetation in Minarimot was classified into 6 communities and 2 subcommunities: Persicaria thunbergii-Isachne globosa community (vegetation type: A), Scirpus tribangulatus-Eleocharis manillata var. cyclocarpa community (B) (Aneilema keisak subcommunity (B-1) and Caldesia parnassifolia-Potamogeton distinctus subcommunity (B-2)), Eleocharis kuroguwai community (C), Phragmites communis community (D), Scirpus tabernaemontani community(E) and Typha orientalis community (F). These communities were grouped into three main categories according to cluster analysis. The community (A) established at the edge of the wetland which has the driest condition was distinguished as Group I, while the community (B) emerged in the submerged zone was distinguished as Group III. The Group II was designated as the communities (C, D, E, F) between Group I and III, whose communities were occasionally submerged. The result of principal coordinate analysis (PCoA) appeared that the different vegetation established along the wetland were depending on water environment such as water depth and the period submerged.

• 농촌지도와개발
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• 제9권1호
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• pp.87-100
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• 2002

The purpose of this study were to compare the community attachment between rural and urban communities, and suggest directions for community development plans of rural and urban communities in Korea. The data for this study were collected from two communities(n=285), one from Gochang representing rural community, located in Jeonbuk province(n=142), and the other from Suwon city representing urban community located in Gyunggi province(n=143), utilizing questionnaire. The major finding of this study were as follows; 1) Community attachment was significantly higher in community than in urban community, and the linear development model was more appropriate to explain the results of the study. 2) Community attachment appeared to be significantly related to social bonds, community participation, social trust, community economical activities, and social and cultural environment. 3)In general, determinants such as number of acquaintances, price of cultural heritages of residence, trust for local government, good traffic environment, job satisfaction, etc. explained 55.5% in rural and urban communities.

• 한국토양비료학회지
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• 제49권6호
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• pp.783-788
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• 2016

Agricultural management of paddy soil depends on the effects of soil microbial activities. The present study evaluated the soil microbial community of 25 paddy soils in Gyeongnam Province by fatty acid methyl ester (FAME). The average of microbial communities in paddy soils were 32.2% of total bacteria, 16.7% of Gram-negative bacteria, 12.9% of Gram-positive bacteria, 2.0% of actinomycetes, 14.9% of fungi, and 1.3% of arbuscular mycorrhizal fungi. The communities of total bacteria (34.9%) and Gram-negative bacteria (19.4%) in soils with $30{\sim}35g\;kg^{-1}$ of organic matter were significantly larger than those in soils with other organic matter levels. However, soils with $20{\sim}30g\;kg^{-1}$ of organic matter had significantly low ratio of cy17:0 to $16:1{\omega}7c$ and cy19:0 to $18:1{\omega}7c$ as compared with soils with $30{\sim}35g\;kg^{-1}$ of organic matter, indicating microbial stress decreased (p < 0.05). In principal component analyses of soil microbial communities, Gram-negative bacteria should be considered as a potential responsible factor for the obvious microbial community differentiation that was observed between the two different organic matter levels in paddy fields. Thus, soils containing $20{\sim}30g\;kg^{-1}$ of organic matter were responsible for strong effect on microbial biomass and stress in paddy fields.

• 한국환경과학회지
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• 제25권10호
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• pp.1407-1415
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• 2016

In this study, we investigated the relationship between noise reduction and the community structure of nine groups of typical plant communities as well as the reduction in noise at different frequencies. The semantic differential method was adopted to explore the perception of noise reduction. The results indicated that there was a significantly positive correlation between noise reduction and coverage, a significantly negative correlation between noise reduction and bifurcate height, and a negative correlation between noise reduction and bare rate. However, there was no significant correlation between noise reduction and height, diameter at breast height, or crown width. The reduction of middle-frequency noise was better than that of low- and high-frequency noise. The indicators "quiet" and "calm" showed that plant communities could reduce the noise perceived by humans. However, overly dense woodland caused nervousness, fear, depression, and other negative effects. Relatively open environments and those with large forest gaps obtained the highest evaluation.