• Korean Journal of Medicinal Crop Science
• /
• v.21 no.6
• /
• pp.444-454
• /
• 2013

The development of random amplified polymorphic DNA (RAPD) and expressed sequence tag-derived simple sequence repeats (EST-SSRs) provided a useful tool for investigating Korean ginseng genetic diversity. In this study, 18 polymorphic markers (7 RAPD and 11 EST-SSR) selected to assess the genetic diversity in 31 ginseng accessions (11 Korean ginseng cultivars and 20 breeding lines). In RAPD analysis, a total of 53 unique polymorphic bands were obtained from ginseng accessions and number of amplicons ranged from 4 to 11 with a mean of 7.5 bands. Pair-wise genetic similarity coefficient (Nei) among all pairs of ginseng accessions varied from 0.01 to 0.32, with a mean of 0.11. On the basis of the resulting data, the 31 ginseng accessions were grouped into six clusters. As a result of EST-SSR analysis, 11 EST-SSR markers detected polymorphisms among the 31 ginseng accessions and revealed 49 alleles with a mean of 4.45 alleles per primer. The polymorphism information content (PIC) value ranged from 0.06 to 0.31, with an average of 0.198. The 31 ginseng accessions were classified into five groups by cluster analysis based on Nei's genetic distances. Consequently, the results of ginseng-specific RAPD and EST-SSR markers may prove useful for the evaluation of genetic diversity and discrimination of Korean ginseng cultivars and breeding lines.

• Journal of Convergence for Information Technology
• /
• v.10 no.11
• /
• pp.168-176
• /
• 2020

Reactive oxygen species (ROS) damage DNA and cause cancer. Therefore, the research is being conducted on the development of antioxidants for the removal of ROS. This study was performed to investigate antioxidant activity and protective effect against oxidative DNA damage using ethyl acetate fractions from the cone of Pinus rigida × taeda (ERT). The antioxidant activity was evaluated using the DPPH, ABTS radical scavenging assay, reducing power assay, and Fe2+ chelating assay. Also, the contents of phenolic compounds and vitamin C related to antioxidant activity were analyzed to confirm phytochemicals. The DNA protective effect against oxidative stress was confirmed by the φX-174 RF I plasmid DNA cleavage assay. As a result, ERT showed DPPH and ABTS radical scavenging activities in a concentration-dependent manner. The results of reducing power and Fe2+ chelating activities were 77.32 ± 2.28% and 64.09 ± 1.01% at 200 ㎍/㎖. Also, ERT showed a DNA protective effect against oxidative stress.

• Journal of Korean Society of Forest Science
• /
• v.94 no.6
• /
• pp.462-467
• /
• 2005

This study aims to classify the biotope types and evaluate its functions for acquiring the basic information which can support the landscape ecological management of the urban forest in the case of the three parks in Daegu metropolitan city and then to analyze vegetation landscape patterns. The biotope classification was mostly divided into 5 to 6 biotope type groups in the high units and 15 to 20 biotope types belong to them and then could know that the more big the park size, the more diverse and large the biotope types and its average area. The biotope grade over the three parks was dominating low rank grade (1 to 3 grade) 72.7% that forest organism is inadequate as live, and high grade (7 to 9 grade) did not appear entirely. Most in Biotope function estimation were appeared less than the middle rank grade and biotope area is bigger, those grade rises and temperature could know that is fallen. Vegetation landscape patterns was very simple because only two kinds of Robinia pseudo-acacia and Quercus acutissima is appeared as the dominated landscape elements in all of the three parks. And also because average area of those elements is generally 1ha or so, there was much problems in consecutiveness of functional role as forest ecosystem component. Conclusively, Ecological landscape management need to improve Biotope diversity and functionality, and it secures ecological minimal area and space linkage.

• KIPS Transactions on Software and Data Engineering
• /
• v.7 no.4
• /
• pp.129-134
• /
• 2018

Recently, the artificial neural network (ANN) model is a promising technique in the prediction, numerical control, robot control and pattern recognition. We predicted the outside temperature of greenhouse using ANN and utilized the model in greenhouse control. The performance of ANN model was evaluated and compared with multiple regression model(MRM) and support vector machine (SVM) model. The 10-fold cross validation was used as the evaluation method. In order to improve the prediction performance, the data reduction was performed by correlation analysis and new factor were extracted from measured data to improve the reliability of training data. The backpropagation algorithm was used for constructing ANN, multiple regression model was constructed by M5 method. And SVM model was constructed by epsilon-SVM method. As the result showed that the RMSE (Root Mean Squared Error) value of ANN, MRM and SVM were 0.9256, 1.8503 and 7.5521 respectively. In addition, by applying the prediction model to greenhouse heating load calculation, it can increase the income by reducing the energy cost in the greenhouse. The heating load of the experimented greenhouse was 3326.4kcal/h and the fuel consumption was estimated to be 453.8L as the total heating time is $10000^{\circ}C/h$. Therefore, data mining technology of ANN can be applied to various agricultural fields such as precise greenhouse control, cultivation techniques, and harvest prediction, thereby contributing to the development of smart agriculture.

• Current Research on Agriculture and Life Sciences
• /
• v.20
• /
• pp.1-8
• /
• 2002

This study was conducted to obtain the information of varietal development using wild soybean through investigation of variation and heredity of major agronomic characters in $F_2$ generation of interspecific cross between Glycine max and G. soja. In segregating populations of two crosses, all characters observed except 100-seed weight showed transgressive segregation. Days to flowering showed normal distribution; mean days to flowering in $F_1$, and $F_2$ was approximately mean of parent. Mean of $F_1$ for 100-seed weight was 6.2g and 5.7g for Eunhakong/KLG10084 and Sohaegnamulkong/KLG10084, respectively, which were somewhat skewed distribution to small seeded parents. Mean seed yield of $F_2$ was about mean of two parents. Degree of vine was 6.4 and 5.7 in $F_1$ for two crosses but it was 6.0 in $F_2$ for Eonbakong/KLG10084, which revealed the degree of vine as partial dominance while 4.6 for Sobaegnamulkong/KLG10084, somewhat different results from the previous cross. Broad-sense heritability($h^2B$) for plant height, days to flowering, pods per plant, seed yield, and degree of vine was comparatively high and narrow-sense heritability($h^2B$) for 100-seed weight which is the moot important character in the development of small seed-size sprout soybean was 52.3% and 65.6% for Eunhakong/KLG10084 and Sohaegnamulkong/KLG10084, respectively, which indicated that selection for the character in early generation was possible.

• Current Research on Agriculture and Life Sciences
• /
• v.5
• /
• pp.112-118
• /
• 1987

This experiment was conducted to obtain the basic information for the effective control of rice blast. Eleven races of Pyricuiaria oryzae were identified, 5 races of KJ group and 6 races of KI group in Kyungpook province. Among them KJ301 was the most dominant one, and they were prevailed in the order of KJ401, KJ201, KJ101, KJ105, KI307 and $KI315^b$. The distribution ratio of KI group race was higher in the middle and northern region than the southern and coastal region. A positive correlation was found between the distribution ratio of KI group races and cultivated area of indica type cultivals. The total ratio of Blasticidin-S resistant isolates in P. oryzae was 31.2%. The distribution ratios of resistant isolates were 43.2% in the southern region, 29.4% middle, 25.0% nothern and 10.5% coastal region, respectively. The distribution ratio of Blasticidin-S resistant Isolates was correlated positively with amounts of the chemical sprayed, but not correlated with the race of P. oryzae.

• Current Research on Agriculture and Life Sciences
• /
• v.2
• /
• pp.29-33
• /
• 1984

From June 28th to July 17th in 1985, this study was performed to obtain the information of the fresh corn forage production through the spraying hydrophonics in summer. The experiments were conducted in vinyl house on Kyungpook University's Experimental Station. The results obtained were as follows: It was suggested that the treatment of soaking for 24 hrs and followed by the pretreatment for 48 hrs was better than other treatments. Seed cost for the production of 1 kg fresh forage was the most inexpensive at the seeding rate of 450 g per $30{\times}60cm$ tray than other seeding rates. Application of 1000 times diluted Hyponex solution (1000X Hyponex) or 300 times diluted Yogen solution (300X Yogen) one time per day resulted in the heavier fresh weight than the other treatments. Treatments of 300X Yogen and 500X Yogen gave the better fresh weight in one application per two days and per three days, respectively. But, this method was not useful in summer because the lowest seed cost for the production 1kg fresh forage was 73Won, and collection of the fresh forage from the mountain range was easy.

• Current Research on Agriculture and Life Sciences
• /
• v.2
• /
• pp.91-97
• /
• 1984

This study was performed to suggest the basic information for the development of covering method for increasing the effect of heat conservation in the unheated vinyl houses. Double and single covered vinyl houses were constructed and the inside temperature variation of the double covered vinyl house was compared to the single covered vinyl house. The results obtained for the vinyl houses were summarized as follows ; 1. With the outside temperature of $-1.0^{\circ}C{\sim}7.5^{\circ}C$ and the solar radiation of maximum $1.02MJ/m^2$, the double covered vinyl house was shown $1.2^{\circ}C$ higher than the single covered vinyl house in the daily mean of inside temperature. 2. The inside temperature of the double covered vinyl house was $2.3^{\circ}C$ higher in the daytime and $1.2^{\circ}C$ higher in the nighttime than that of the single covered vinyl house. 3. The inside temperature of the vinyl house were expressed by the formular (1)-(6) with the outside temperature and by the formular (7)-(10) with the solar radiation. 4. In the effect of heat conservation based on the outside temperature, the double covered vinyl house was 1.2 times in the daytime and 1.76 times in the nighttime in comparison with the single covered vinyl house.

• Current Research on Agriculture and Life Sciences
• /
• v.22
• /
• pp.41-48
• /
• 2004

This study is performed in order to provide the basic information needed for the development of cutting blade for combine. By comparing the developed cutting blade of which were made groove of the surface with the common cutting blade. Friction force and cutting resistance by the cutter bar speed ratio and moisture content of rice stalk were shown as follows: 1. The friction force of the developed cutting blade is shown at the average value $0.12kg{\cdot}m$ as 25% lower than one of the common blade at the average value $0.16kg{\cdot}m$. 2. The cutting resistance of the developed cutting blade is shown as respectively 12%, 15% and 20% lower than one of the common blade at the moisture content of rice stalk of 14.2%, 55.3% and 84.2%. 3. The cutting resistance of cutting blade by two different driving types is shown that the one by double driving type is $0.2kg{\cdot}m$, and the one by single driving type is $0.24kg{\cdot}m$. 4. Optimum cutter bar speed ratio of cutting blade is shown as different according to the moisture content of rice stalk.

• Current Research on Agriculture and Life Sciences
• /
• v.22
• /
• pp.19-27
• /
• 2004

Cultivation area of garlics is 9% of all cultivation area of vegetables. The amount of annual demand is increased in 1,000~1,500ton. Also, the amount of demand per person has a tendency to increase as above 10kg. So, garlics has become important crops in agriculture. The purpose of this study is to acquire the basic information to design the garlic seeder attached power tiller. We used Working Model 3D program to design an automatic aligning device which is the most important part of the garlic seeder. The results are as follows; 1. The optimum depth of garlics seeding was shown as 3cm. 2. The garlic seed has to he discharged from the garlic seeder at intervals of 0.75sec in order to seed at intervals of seeds of 15cm. 3. The optimum design factors of the automatic aligning device were shown as cylinder diameter of 4cm, cylinder gap of 1cm, revolution of 36rpm and inclined angle of cylinder of $8.4^{\circ}$.