• Title/Summary/Keyword: Agricultural R and D

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The Artificial Cultivation of Pleurotus eryngii (I) -Investigation of Mycelial Growth Conditions- (Pleurotus eryngii(큰느타리버섯) 균의 인공재배(I) -균사배양 조건에 관하여-)

  • Kim, Han-Kyoung;Cheong, Jong-Chun;Chang, Hyun-You;Kim, Gwang-Po;Cha, Dong-Yeul;Moon, Byung-Ju
    • The Korean Journal of Mycology
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    • v.25 no.4 s.83
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    • pp.305-310
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    • 1997
  • A study was conducted to obtain optimal mycelial growth conditions of Pleurotus eryngii. Mycelial growth was best on medium Lilly, temperature $25{\sim}30^{\circ}C$, and pH 6.0. Optimal carbon sources were $3{\sim}4%$ glucose and 5% dextrin. Casamino acid 0.12% in medium was good for mycelial growth as a nitrogen source.

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Combustion Qualities of Waste Tire Oil as Substituting Light Oil for Greenhouse Heating (시설농업난방 경유대체유로서 폐타이어오일의 연소 특성)

  • 김영중;유영선;강금춘;이건중;윤진하
    • Journal of Biosystems Engineering
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    • v.25 no.6
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    • pp.481-488
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    • 2000
  • This study was initiated in order to find alternative fuel substituting for light oil the most common fuel for heating greenhouse. The tire oil used in this research was produced by pyrolysis process, one of the final products besides steel string and carbon black in which waste tires as a form of chopped pieces broken by shredding machine are heated up to 200~30$0^{\circ}C$ with maximum restraining of oxygen supply. In order to justify light oil equivalent qualities in tire oil combustion characteristics were defined in the way of comparing kinetic viscosities in the wide range of temperature flame sizes and exhaust gas components in the various combustion conditions. We found that kinetic viscosity of tire oil was lower than light oil by 1 to 2 cSt in the temperature range showing better flowing mobility in the fuel line of the burner and no significant difference in flame size between the two oils in the all combustion treatments. However much more NO and SO$_2$ were detected from the exhaust gases of tire oil than light oil combustions. In fact tire oil contains more nitrogen and total sulfur, by 25 times and 40 times respectively than light oil according to the composition analysis. Tolerable limit for SO$_2$discharge amount defined by the national air pollution standards is under 540ppm so tire oil combustion satisfies the requirement though. It is desirable if sulfur and nitrogen filtering process shall be added in the tire oil production line. Except the exhaust gas components all greenhouse heating qualities of tire oil including hot air temperature are very identical to those of light oil.

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First Report of Root Rot Caused by Plectosphaerella cucumerina on Cabbage in China

  • Li, Pan-Liang;Chai, A-Li;Shi, Yan-Xia;Xie, Xue-Wen;Li, Bao-Ju
    • Mycobiology
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    • v.45 no.2
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    • pp.110-113
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    • 2017
  • Severe root rot was observed in fields of cabbages (Brassica oleracea L.) in 2015 in China. Cardinal symptoms of this disease included root rot and wilting leaves. A fungus was isolated from diseased tissues consistently. Based on the morphological features and molecular analysis of the ITS-5.8S rDNA and D1/D2 domain of the 28S rRNA gene, it was identified as Plectosphaerella cucumerina. This is the first report of P. cucumerina causing cabbage root rot in China and the world.

Detection of Novel Genetic Variations of the MG1R * 3 Allele in Pig(Sus scrofa) (돼지 Melanocortin Receptor 1(MC1R) 대립유전자 3의 신규 유전변이 탐색)

  • Cho, I.C.;Jeong, Y.H.;Jung, J.K.;Seong, P.N.;Oh, W.Y.;Ko, M.S.;Kim, B.W.;Lee, J.G.;Jeon, J.T.
    • Journal of Animal Science and Technology
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    • v.46 no.1
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    • pp.1-6
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    • 2004
  • This study was conducted to investigate novel genetic variations of MCIR^*3 allele. In general, white spotting or white belt on a black backgroud in pigs is determined by the E$^p$ allele at the MCIR/Extention locus. E$^p$ shares a frameshift mutation with the E$^{D2}$ allele for dominant black color. An oligonucleotide primer set was designed to amplify complete coding sequence of the porcine MCIR gene. The MCIR coding sequences obtained from five breeds those were Landrace(white). Yorkshire(white), Hampshire(belt), Berkshire(spot) and Jeju native black pigs(black), were used for this study. A multiple sequence alignment of the MCIR coding region using Clustal W was performed. The total length of the MCIR coding sequence ranged from 963 to 966 base pairs(bp) among the selected breeds. The sequence analysis of the complete coding region of MCIR was revealed that Hampshire and Jeju native black pig have 3 cytosines deletion and Birkshire has 2 cytosines deletion at codon 23(nt68) in Extention loci. Besides the finding, there were three different missense mutations and a frameshift mutation in the MCIR coding region.

Analysis of Policy Priorities for Training Agro-healing Experts Using the AHP Method (치유농업 전문가 양성을 위한 정책 우선순위 분석)

  • Hong, Ji-Young;Lee, Byung-Oh
    • Journal of Agricultural Extension & Community Development
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    • v.23 no.4
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    • pp.419-429
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    • 2016
  • This study focuses on the policy priorities for training agro-healing experts. Training agro-healing experts is an important task that should boost local community in rural areas. In order to make an efficient decision making, the study analyses priority of relevant policies using the AHP(Analytic Hierarchy Process) method. According to the results, R&D comes out first. Human Resource Development(HRD), financial support, and the construction of legal system comes after R&D. In R&D, qualifications and guidelines for participants comes out the most significant issue. In the aspect of HRD, it is very important to develop and utilize regional personnel such as unemployed youth and retirees from related fields. As for financial support, funding for educational facilities (i.e. classrooms and farmland for practice) is needed. In case of legal system, it is desirable to introduce the recruiting experts system and qualification system certified by government.

Effects of Biocontrol Agents on Weed Control, Abundance of Aquatic Animals and Insects, and Yield in Paddy Rice Fields (생물자원의 논 잡초 생물학적 방제 효과와 그에 따른 수서동물과 곤충 종수 및 벼 수량 변화)

  • Hyo Jung Choi;Sang Young Seo;Seon U Choi;Chang Kyu Lee;Mi Jeong Uhm;Ju Hee Kim;Min Sil An;Woo Jung Choi
    • Korean Journal of Environmental Agriculture
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    • v.42 no.1
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    • pp.63-70
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    • 2023
  • Golden apple snails (Pomacea canaliculat) (GAS) are widely used for weed control in rice cultivation. However, concerns on the ecological risk of invasive GAS species are increasing. This study aimed to evaluate the overall impacts of GAS on weed control, abundance of aquatic animals and insects, and rice yield, in comparison with that from other biocontrol agents such as loach (Misgurnus mizolepis) and catfish (Silurus asotus), which are alternatives for biological weed control in rice paddy cultivation. Field experiments included five treatments; control, herbicide, GAS, loach, and catfish. During the rice growth, weed appearance and biological abundance were monitored, and at harvest, the rice yield was determined. Weed control efficiency was the highest for GAS treatment (100%), followed by that for herbicide (95.8%), loach (57.5%), and catfish treatments (31.7%). Insect abundance was considerably decreased in GAS treatment due to heavy weed removal, which affects the habitat of aquatic animals and insects. The amount of rice yield (unit: kg 10 a-1) was in the order of GAS (798.9) > loach (708.1) = herbicide (700.7) > catfish (629.4) > control (496.0). Therefore, considering the weed control efficiency, biological abudance, and rice yield, loach could be a potential alternative for biological weed control in organic rice farming. However, the activity of the biocontrol agents are susceptible to environmental conditions; therefore, further studies under different conditions are essential to confirm these findings.

Isolation and Structure Identification of Antibacterial Substances from the Rhizome of Zingiber mioga Roscoe (양하의 근경에서 항균성 물질 분리 및 구조동정)

  • Kim, Seong-Cheol;Song, Eun-Young;Kim, Kong-Ho;Kwon, Hyeog-Mo;Kang, Sang-Heon;Park, Ki-Hun;Jung, Yong-Hwan;Jang, Ki-Chang
    • Applied Biological Chemistry
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    • v.46 no.3
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    • pp.246-250
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    • 2003
  • In order to isolate antibacterial substances from the rhizome of Zingiber mioga Roscoe, the ethanol extracts was fractionated according to the activity against Bacillus subtilis, B. cereus and Staphylococcus aureus. Three antibacterial substances were isolated and purified by column chromatography and recrystallization. Compounds I and III showed activity against all the tested bacterias and compound II exhibited the activity against B. subtilis and B. cereus S. aureus. Compound I was examined antimicrobial activity against B. subtilis, B. cereus and S. aureus by optical density using Bioscreen C. Compound I showed strong growth inhibition at 10 ppm on B. subtilis and B. cereus for 72 hrs, and at 25 ppm on S. aureus. On the basis of spectrometric studies including $1^H-NMR$, ${13}^C-NMR$, DEPT, IH-lH COSY, HMQC, HMBC and IR, compounds I, II and III were identified as $(E)-8{\beta}(17)-epoxylabd-12-ene-15,16-dial\;(C_{20}H_{30}O_3,\;MW=318)$, galanolactone $(C_{20}H_{30}O_3\;MW=318)$ and galanal A $(C_{20}H_{30}O_3,\;MW=318)$, respectively. These results are the first reports on the isolation of $(E)-8{\beta}(17)-epoxylabd-12-ene-15,16-dial, galanolactone and galanal A from the rhizome of Zingiber mioga.

Peroxisome Proliferator-activated Receptor${\gamma}$ Is Involved in Weaning to Estrus of Primiparous Sows by Regulating the Expression of Hormone Genes in Hypothalamus-pituitary-ovary Axis

  • Kong, L.J.;Wang, A.G.;Fu, J.L.;Lai, CH.H.;Wang, X.F.;Lin, H.CH.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.3
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    • pp.340-350
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    • 2007
  • The objective of this study was to determine whether peroxisome proliferator-activated receptor ${\gamma}$(PPAR${\gamma}$ is involved in the regulation of weaning to estrus of primiparous sows. Twelve sows composed of 6 groups of 2 full-sibs in a similar age (325.2 d), body weight (BW; 152.4 kg) and backfat thickness (BFT; 27.0 mm) at start of lactation, were allocated to accept 31 MJ (restricted group, R-group) or 53 MJ (control group, C-group) DE/d treatment, respectively. The experimental results indicated that the low energy intake resulted in excessive losses of BW and BFT during lactation in R-group sows, which may be related to decrease of serum 15-deoxy-${\Delta}^{12,14}$-prostaglandin $J_2$ (15d-$PGJ_2$), a ligand of PPAR${\gamma}$ The obvious peak and the frequency of LH, FSH and estradiol ($E_2$) were only observed in C-group sows. Except for $E_2$ at d 1 and 2, serum FSH, LH and $E_2$ concentrations in R-group were lower than those in C-group sows after weaning. However, the serum progesterone ($P_4$) level in R-group sows was always more than that in C-group. The expression abundances of PPAR${\gamma}$and GnRH receptor (GnRH-R) in pituitary, FSH receptor (FSH-R), LH receptor (LH-R), estrogen receptor (ES-R) and aromatase in ovary of anestrous sows were lower than those of estrous sows. Neither the BFT nor the BW was associated with the mRNA abundance of PPAR${\gamma}$in hypothalamus during lactation. Expressions of PPAR${\gamma}$in pituitary and ovary were affected evidently by the BFT changes and only by the loss of BW of sows during and after lactation. Furthermore, PPAR${\gamma}$mRNA level in ovary was significantly related to the expression abundances of GnRH-R, FSH-R, ES-R and aromatase, and GnRH-R was obviously associated with PPAR${\gamma}$expression in pituitary. However, PPAR${\gamma}$expression in hypothalamus likely has no effects on these genes expression and no obvious difference for all sows. Not serum $E_2$ or $P_4$ alone but the ratios of $E_2$ to $P_4$ and 15d-$PGJ_2$ to $P_4$, and serum FSH and LH were evidently related to PPAR${\gamma}$expression in pituitary and ovary. It is concluded that PPAR${\gamma}$is associated with body conditions, reproduction hormones and their receptor expression, which affected the functions of pituitary and ovary and ultimately the estrus after weaning of primiparous sows.