Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Detection of Novel Genetic Variations of the MG1R * 3 Allele in Pig(Sus scrofa)

돼지 Melanocortin Receptor 1(MC1R) 대립유전자 3의 신규 유전변이 탐색

  • Cho, I.C. (National Jeju Agricultural Experiment Station, R.D.A) ;
  • Jeong, Y.H. (National Jeju Agricultural Experiment Station, R.D.A) ;
  • Jung, J.K. (National Jeju Agricultural Experiment Station, R.D.A) ;
  • Seong, P.N. (National Jeju Agricultural Experiment Station, R.D.A) ;
  • Oh, W.Y. (National Jeju Agricultural Experiment Station, R.D.A) ;
  • Ko, M.S. (National Jeju Agricultural Experiment Station, R.D.A) ;
  • Kim, B.W. (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, J.G. (Division of Applied Life Science, Gyeongsang National University) ;
  • Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University)
  • 조인철 (농업진흥청 제주농업시험장) ;
  • 정용환 (농업진흥청 제주농업시험장) ;
  • 정진관 (농업진흥청 제주농업시험장) ;
  • 성필남 (농업진흥청 제주농업시험장) ;
  • 오운용 (농업진흥청 제주농업시험장) ;
  • 고문석 (농업진흥청 제주농업시험장) ;
  • 김병우 (경상대학교 응용생명과학부) ;
  • 이정규 (경상대학교 응용생명과학부) ;
  • 전진태 (경상대학교 응용생명과학부)
  • Published : 2004.02.29
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Abstract

This study was conducted to investigate novel genetic variations of MCIR^*3 allele. In general, white spotting or white belt on a black backgroud in pigs is determined by the E$^p$ allele at the MCIR/Extention locus. E$^p$ shares a frameshift mutation with the E$^{D2}$ allele for dominant black color. An oligonucleotide primer set was designed to amplify complete coding sequence of the porcine MCIR gene. The MCIR coding sequences obtained from five breeds those were Landrace(white). Yorkshire(white), Hampshire(belt), Berkshire(spot) and Jeju native black pigs(black), were used for this study. A multiple sequence alignment of the MCIR coding region using Clustal W was performed. The total length of the MCIR coding sequence ranged from 963 to 966 base pairs(bp) among the selected breeds. The sequence analysis of the complete coding region of MCIR was revealed that Hampshire and Jeju native black pig have 3 cytosines deletion and Birkshire has 2 cytosines deletion at codon 23(nt68) in Extention loci. Besides the finding, there were three different missense mutations and a frameshift mutation in the MCIR coding region.

본 연구는 MCIR$^*$3 allele의 돼지에 있어서 유전적 변이를 관찰하기 위하여 수행하였다. 일반적으로 흑모색 바탕에 백색반점이나 백색띠를 갖고 있는 돼지의 MCIR 유전자의 유전자형은 E$^{D2}$로 나타낸다. 우성 백색계통의 E$^P$ 유전자형은 우성 흑모색 계통의 E$^{D2}$ 유전자와 frameshift mutation 관계가 있다. 돼지 MCIR 전체 번역지역을 증폭하기 위하여 oligonucleotide primer률 제작하여 PCR을 수행 하였다. 그 결과 길이가 963${\sim}$966 base pairs인 돼지 MCIR 유전자의 전체번역지역을 포함하는 산물을 얻었다. 이들 번역부위의 염기서열 결정하고 이들을 Clusta1 W 프로그램을 이용하여 정렬한 결과 23번 코돈{nt68)에서 Hampshire와 제주 재래혹돈은 염기 시토신(cytosine)이 3 개 그리고 Birl‘shire의 경우 염기 시토신(cytosine)이 2개 결실되어 있었다. 그 외에 3개의 missense mutations과 하나의 frameshift mutation이 발견되었다.

Keywords

References

  1. Evert, R. E., Routhuizen, J. and va Oost, B A. 2000. Identification of a premature stop codon in the melanocyte-stimulating hormone receptor gene(MCIR) in labrador and golden retrievers with yellow coat color. Animal Genetics 31:194-199.
  2. Giuffra, E., Kijas, J. M. H., Amarger, V., Carlberg, O., J.-T. Jeon. and Andersson, L. 2000. The origin of the domestic pig: Independent domestication and subsequent introgression. Genetics 154:1785-1791.
  3. Jackson. I. J. 1993. Color-coded switches. Nature 362:587-588.
  4. Kijas, J. M. H.. Wales. R., Tornten, A., Chardon, P., Moller M. and Andersson. L. 1998. Melanocortin receptor I(MCIR) mutation and coat color in pigs. Genetics 150: 1177-1185.
  5. Kijas, J. M. H., Moller. M., Plastow, G. and Andersson. L. 2001. A frarneshift mutation in MCIR and a high frequency of somatic reversions cause black spotting in pigs. Genetics 158:779-785.
  6. Klungland, H., Vage. D. I., Gomez. R. L., Adalsteinsson. S. and Lien, S. 1995. The role of melanocyte-stimulating hormone(MSH) receptor in bovine coat color determination. Mamm. Genome 6:636-639.
  7. Marklund. L., Johansson, M., Sandberg, K. and Andersson, L. 1996. A missense mutation in the gene for melanocyte-stimulating hormone receptor (MCIR) is associated with the chestnut coat color in horses. Mamm. Genome 7:895-899
  8. Newton, J. M., Wilkie, A. L., He, L., Jordan. S. A. and Metallinos, D. L. 2000. Melanocortin I receptor variation in the domestic dog. Mamm. Genome 11:24-30 https://doi.org/10.1007/s003350010005
  9. Robbins. L. S., Nadeau, J. H., Johnson. K. R., Kelly, M. A. and Roselli Rehfuss, L. 1993. Pigmentation phenotypes of variant Extension locus alleles result from point mutations that alter MSH receptor function. Cell 72:827-834.
  10. Takeuchi, S. H., Suzuki, H., Yabuuchi, M. and Takahashi, S. 1996. A possible involvement of melanocortin I-receptor in regulating feather color pigmentation in the chiken. Biochim. Biophys. Acta. 1308:164-168.
  11. Thompson. J. D.. Higgins. D. G. and Gibson, T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting position specific gap penalties and weight matrix choice. Nucl. Acids Res. 22:4673-4680. https://doi.org/10.1093/nar/22.22.4673
  12. Vage, D. 1.. Lu, D., Klungland. H.. Lien. S. and Adalsteinsson. S. 1997. A non-epistatic interaction of Agouti and Extension in the fox, Vulpes vulpes. Nat. Genet. 15:311-315.
  13. Vage, D. I., Klungland, H. and Lu, D. 1999. Molecular and pharmacological characterization of dominant black coat color in sheep. Mamm. Genome 10:39-43.
  14. Valverde, P., Healy, E., Jackson, I.. Rees, J. L. and Thody, A. J. 1995. Variants of the melanocytestimulating hormone receptor gene are associated with red hair and fair skin in humans. Nat. Genet. 11:328-330. https://doi.org/10.1038/ng1195-328
  15. 김태헌, 윤두학, 노환국, 최봉환, 연성흠, 김남순, 이화진, 정일정, 한재용. 2001. 재래돼지 Melanocortin Receptor 1(MCIR)의 변이에 관한 연구. 한국동물자원과학회지 43(3):293-302
  16. 조인철, 이정규, 정진관, 양보석, 강승률, 김병우, 2002. 랜드레이스, 대요크셔, 듀록 및 제주 흑돈의 Melanocortin 1 Receptor(MCIR) 유전자의 유전자형 분석. 한국동물자원과학회지 44(2):207-212.

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