• Title/Summary/Keyword: Advanced Construction Technology

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Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing

  • Lee, Minho;Choi, Shin-Jung;Han, Sangjo;Nam, Miyoung;Kim, Dongsup;Kim, Dong-Uk;Hoe, Kwang-Lae
    • Genomics & Informatics
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    • v.16 no.2
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    • pp.22-29
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    • 2018
  • Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out of 8,708 barcodes (4,354 strains) covering 88.5% of all 4,919 open reading frames, 7,734 barcodes (88.8%) were validated as high-fidelity to be inserted at the correct positions by Sanger sequencing. Sequence examination of the 7,734 high-fidelity barcodes revealed that 1,039 barcodes (13.4%) deviated from the original design. In total, 1,284 mutations (mutation rate of 16.6%) exist within the 1,039 mutated barcodes, which is comparable to budding yeast (18%). When the type of mutation was considered, substitutions accounted for 845 mutations (10.9%), deletions accounted for 319 mutations (4.1%), and insertions accounted for 121 mutations (1.6%). Peculiarly, the frequency of substitutions (67.6%) was unexpectedly higher than in budding yeast (~28%) and well above the predicted error of Sanger sequencing (~2%), which might have arisen during the solid-phase oligonucleotide synthesis and PCR amplification of the barcodes during strain construction. When the mutation rate was analyzed by position within 20-mer barcodes using the 1,284 mutations from the 7,734 sequenced barcodes, there was no significant difference between up-tags and down-tags at a given position. The mutation frequency at a given position was similar at most positions, ranging from 0.4% (32/7,734) to 1.1% (82/7,734), except at position 1, which was highest (3.1%), as in budding yeast. Together, well-defined barcode sequences, combined with the next-generation sequencing platform, promise to make the fission yeast gene deletion library a powerful tool for understanding gene function.

Key Risks and Success Factors on the China's Public-Private Partnerships Water Project (중국 수처리 민관협력사업 사례분석을 통한 시사점 도출: 위험 및 성공 요인 도출)

  • Choi, Jae-Ho;Lee, Seung-Ho
    • Korean Journal of Construction Engineering and Management
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    • v.11 no.3
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    • pp.134-144
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    • 2010
  • In China, the enhancement of water services has become the most crucial issue confronted with the rapid urbanization and industrialization process. A huge financial gap to meet the demand for water infrastructure and need for adopting advanced operation technology precipitated the rapid growth of PPP over the last 10 years. Diverse schemes of PPP such as TOT, Divestiture, and Management Contract and Lease have been practiced. Local governments and private investors/operator have adjusted their objectives and strategies to avoid potential pitfalls behind BOT projects in China. However, current academic research outcomes do not properly reflect important issues of BOT projects or related case studies in China. This limitation has brought in the lack of assessment of important risks and success factors required for the improvement of the body of risk management. In this regard, this study uses the market analysis method to identify major schemes of PPP water projects and conducts case studies on five PPP projects to identify key risk and success factors in association with each different scheme. It is expected that the risk and success factors identified from the cases will be used as reference to Korean companies which plan to enter the Chinese water market.

A Study on Preservation of Disaster from Earthquake for Kori Nuclear Power Plant -In terms of Ubiquitous Administrative Spatial Informatization System and Smart Ecological City- (고리원전과 지진재난방재 연구 -스마트 생태도시와 유비쿼터스 행정공간정보화 구축측면에서-)

  • Lee, Sang-Yun;Yoon, Hong-Joo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.12 no.2
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    • pp.243-254
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    • 2017
  • Recently, discussions about the guarantee of smart ecological environment have been started in S. Korea. These discussions are becoming more and more popular in the aspect of ubiquitous administrative spatial informatization in utilization using big data as a new paradigm due to the rapid change of information and communication technology, such as the start of smart society and the ubiquitous era. In addition, there is a growing interest in discussing environmental and disaster preservation in terms of ubiquitous smart city construction in smart society. In thisstudy, by applying 'scenario planning' as a foresight method, we have developed a desirable future vision for ubiquitous administrative spatial informatization in terms of preservation of disaster of Kori nuclear power plant like earthquake. In order to establish a high level of city disaster prevention level in S. Korea in 2030 when the big data and big data System will be further intensified in the future, it is necessary to develop advanced ICT city disaster prevention system with big data administrative spatial informatization in terms ofsmart ecological city construction.

Development of Flood Rapid Defense System(FRDS) suitable for Southeast Asian Disaster (동남아시아 재난에 적합한 도심형 홍수임시차수시스템 개발)

  • Jung, In-Su;Oh, Eun-Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.11
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    • pp.8-17
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    • 2018
  • A large urban region in Bangkok, Thailand is often inundated due to shallow water floods along the paved roads that have poor drainage facilities, and that can cause urban flooding. Existing methods, including using sand bags are not effective to prevent flooding in urban areas where the amount of sand is not sufficient. Thus, it is necessary to install artificial flood defense structures. However flooding and overflow defense equipment, which was developed in some advanced nations in Europe and in the USA, is highly expensive and complex construction methods are needed, therefore they are not suitable to be used in Southeast Asia. Thus, it is necessary to develop a flood rapid defense system(FRDS), which is inexpensive and simple to build, but is also highly functional. Thus, this study developed an FRDS that can be applied to Southeast Asia through the careful study of FRDS overviews, an analysis on the development trends in Korea and overseas, and the proposal of development needs and directions of the region. For the system developed, Korean Standards(KS) performance evaluations on leakage ratio deformation tests and impact resistance tests were conducted at the Outdoor Demonstration Test Center(Seosan) in the Korea Conformity Laboratories(KCL) and the system satisfied the standards of KS F 2639(leakage and deformation test) and KS F 2236(impact resistance test). The present study results can not only be applied to urban floods in Southeast Asian nations to cope with flood-related disasters, but also be utilized in flood prone regions and for major facilities in Korea. They can also induce scientific and pro-active responses from major local governments and facility management organizations in relation to urban floods.

Rheological Properties of Cement Paste Mixed with Aqueously Dispersed Single-Walled Carbon Nanotubes (Single-Walled 탄소나노튜브 수용액 혼입 시멘트 페이스트의 유변학적 특성)

  • Kim, Ji-Hyun;Chung, Chul-Woo
    • Journal of the Korea Institute of Building Construction
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    • v.19 no.2
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    • pp.113-121
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    • 2019
  • Single walled carbon nanotube (SWCNT) has been used as a material for reinforcing various advanced materials because it has superior mechanical properties. However, pure SWCNT that does not have any functional group has a hydrophobic character, and exists as bundles due to the strong Van der Waals attraction between each SWCNT. Due to these reasons, it is very difficult to disperse SWCNTs in the water. In this work, in order to use SWCNT for production of cementitious composites, SWCNT was first dispersed in water to make an aqueous solution. Sodium deoxycholate (DOC) and Sodium dodecyl sulfate (SDS) were chosen as surfactants, and the dosage of DOC and SDS were 2wt% and 1wt%, respectively. Sonication and ultracentrifugation were applied to separate each SWCNT and impurities. Using such processed SWCNT solutions, cement paste was prepared and its shear stress vs. strain rate relationship was studied. The yield stress and plastic viscosity of cement paste were obtained using Bingham model. According to the results in this work, cement pastes made with DOC and SDS showed similar rheological behavior to that of air entrained cement paste. While cement paste made with DOC 2 wt.% SWCNT solution showed similar rheological behavior to that of plain cement paste, cement paste made with SDS 1 wt.% SWCNT solution showed different rheological behavior showing much less yield stress than plain cement paste.

Development of Pozzolanic material from clay

  • Alaskar, Abdulaziz;Shah, S.N.R.;Keerio, Manthar Ali;Phulpoto, Javed Ali;Baharom, Shahrizan;Assilzadeh, Hamid;Alyousef, Rayed;Alabduljabbar, Hisham;Mohamed, Abdeliazim Mustafa
    • Advances in concrete construction
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    • v.10 no.4
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    • pp.301-310
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    • 2020
  • The following paper concentrates on the objective of studying the influences of extent of duration and temperature on the Pozzolanic properties as well as reactivity of locally existing natural clay of Nai Gaj, district Dadu, Sindh Pakistan. The activation of the clay only occurs through heating when temperature in a furnace chamber reaches 600, 700 and 800oC for 1, 2 and 3 hours and at 900 and 1000℃ for 1 and 2 hours. Furthermore, the strength activity index (SAI) of advanced pozzolanic material happens to be identified through 20% cement replacement for different samples of calcined clay as per ASTM C-618. The compressive strength test of samples had been operated for 7 and 28-days curing afterwards. The maximum compressive strength had been seen in mix E in which cement was replaced with clay calcined at 700℃ for 1 hour that is 27.05 MPa that is 24.31% more than that of control mix. The results gathered from the SAI verdicts the optimal activation temperature is 700℃ within a one-hour time period. The SAI at a temperature of 700℃ with a one-hour duration at 28 days is 124.31% which happens to satisfy the requirements of the new Pozzolanic material, in order to be applied in mortar/concrete (i.e., 75%). The Energy- dispersive spectrometry (EDS) along with the X-ray diffraction (XRD) have been carried out in means of verifying whether there is silica content or amorphous silica present in metakaolin that has been developed. The findings gathered from the SAI were validated, as the analysis of XRD verified that there is in fact Pozzolanic activity of developed metakaolin. Additionally, based on observation, the activated metakaolin holds a significant influence on the increase in mortar's compressive strength.

Strategies for Cyber Territory Construction (사이버국토 구축전략에 관한 연구)

  • 김영표;한선희
    • Spatial Information Research
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    • v.10 no.1
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    • pp.1-14
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    • 2002
  • Due to the rapid development in information technology, countries of advanced information technology and multi-national information communication industries have expanding their invesment in constructing a cyber territory or a cyber city, and even a cyber globe that combines the real world with the cyber world. As such, in order to keep up with the competition with other countries to secure the Invisible Continent, Korea cannot afford to fall behind in the preparation and efforts to construct a cyber territory. Therefore in preparation for the inevitable establishment of a cyber territory, this study defined the concept of a cyber territory clearly and proposed a promotional strategy needed by the government for the establishment of a cyber territory. A cyber territory is 'the dynamic second territory that realizes various values in various aspects through organically combining innumerable active bodies in a surreal space and that are created by systematically and optimally connecting the physical topographical space and activity of the first nation to the cyberspace'. To explain further, a cyber territory is defined as another simulated space not only to manage the land systematically and deal with administrative services far the greater population, but also to contain economic activities of corporations and the citizens' everyday lives in a virtual reality by digitizing the entire territory including even the sea. In order to establish such cyber territory, it is necessary to revise related laws and policies, to foster related technology and industry as a main engine for national development, to promote public awareness, and to train related human resources.

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The Effects of Wind Power Generation Exports on the National Economy (풍력발전 해외수출의 경제적 파급효과 분석)

  • Jin, Se-Jun;Jeong, Dong-Won;Kwon, Yong-O;Yoo, Seung-Hoon
    • Journal of Energy Engineering
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    • v.21 no.3
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    • pp.281-291
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    • 2012
  • Recently, global economy has recovered and aspects of the renewable energy industry in the global competition is more fierce, the new growth engines of the major countries, including the United States and China, industry promotion policy as being deployed. Major advanced countries and Korea also invested a lot of money to wind power development as a part of renewable energy development and promoting the construction of wind power generation. The global wind power generation market is expected to further increase the scale to about 70 billion US dollars, thus, Korea as well as the installation of domestic wind power overseas actively considering. This study uses input-output analysis to estimate the role of wind power generation sector exports national economy. More specifically, this study shows what national economy effect of production-inducing effect, value-added inducing effect, and employment-inducing effect are explored with demand-driven moel. After define wind power generation sector what small sized of Input-Output table 168 sectors among 11 sectors, this study pays particular and close attention to wind power generation sector by taking the sector as exogenous specification and then investigating economic impacts of it. The wind power generation exportation case of overseas 100 billion won, production-inducing effect, value-added inducing effect, and employment-inducing effect are 205 billion won, 68 billion won and 1,054 persons, respectively. These quantitative information can be usefully utilized in the policy-making for the industrialization of wind power generation exports.

Methodology for Estimating the Probability of Damage to a Heat Transmission Pipe (열수송관 파손확률 추정 방법론 개발)

  • Kong, Myeongsik;Kang, Jaemo
    • Journal of the Korean GEO-environmental Society
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    • v.22 no.11
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    • pp.15-21
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    • 2021
  • Losses of both life and property increased from damage to underground pipe such as heat transmission pipe buried underground in downtown because pipes are gradually aging. Considering the characteristics of the heat transmission pipe, which is not exposed to the outside and difficult to immediately identify problems such as damage, it is realistic to indirectly check the condition of the facility based on the historical information that is periodically collected through facility maintenance. In this study, a methodology for estimating the damage probability was developed by examining the history information of the heat transmission pipe, deriving an evaluation factor that is related to the damage probability. The contribution factor of the damage probability were reviewed by analyzing not only the guidelines for maintenance of heat transmission pipe of advanced European countries and domestic district heating companies, but also the cases of waterworks with similar characteristics. Evaluation factors were selected by considering not only the correlation with the damage probability but also the possibility of securing data. Based on 1999, when the construction technology and standards of heat transmission pipe changed, the damage probability estimation function according to the period of use was divided into the case of being buried before 1998 and the case of being buried after 1999, and presented. In addition, the damage probability was corrected by assigning weights according to the measured data for each evaluation factor such as the diameter, use, and management authority.

Analysis of Optimal Locations for Resource-Development Plants in the Arctic Permafrost Considering Surface Displacement: A Case Study of Oil Sands Plants in the Athabasca Region, Canada (지표변위를 고려한 북극 동토 지역의 자원개발 플랜트 건설 최적 입지 분석: 캐나다 Athabasca 지역의 오일샌드 플랜트 사례 연구)

  • Taewook Kim;YoungSeok Kim;Sewon Kim;Hyangsun Han
    • The Journal of Engineering Geology
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    • v.33 no.2
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    • pp.275-291
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    • 2023
  • Global warming has made the polar regions more accessible, leading to increased demand for the construction of new resource-development plants in oil-rich permafrost regions. The selection of locations of resource-development plants in permafrost regions should consider the surface displacement resulting from thawing and freezing of the active layer of permafrost. However, few studies have considered surface displacement in the selection of optimal locations of resource-development plants in permafrost region. In this study, Analytic Hierarchy Process (AHP) analysis using a range of geospatial information variables was performed to select optimal locations for the construction of oil-sands development plants in the permafrost region of southern Athabasca, Alberta, Canada, including consideration of surface displacement. The surface displacement velocity was estimated by applying the Small BAseline Subset Interferometric Synthetic Aperture Radar technique to time-series Advanced Land Observing Satellite Phased Array L-band Synthetic Aperture Radar images acquired from February 2007 to March 2011. ERA5 reanalysis data were used to generate geospatial data for air temperature, surface temperature, and soil temperature averaged for the period 2000~2010. Geospatial data for roads and railways provided by Statistics Canada and land cover maps distributed by the North American Commission for Environmental Cooperation were also used in the AHP analysis. The suitability of sites analyzed using land cover, surface displacement, and road accessibility as the three most important geospatial factors was validated using the locations of oil-sand plants built since 2010. The sensitivity of surface displacement to the determination of location suitability was found to be very high. We confirm that surface displacement should be considered in the selection of optimal locations for the construction of new resource-development plants in permafrost regions.