• Journal of Microbiology and Biotechnology
• /
• 제31권9호
• /
• pp.1311-1322
• /
• 2021

Microbially induced calcium carbonate precipitation (MICP) has recently become an intelligent and environmentally friendly method for repairing cracks in concrete. To improve on this ability of microbial materials concrete repair, we applied random mutagenesis and optimization of mineralization conditions to improve the quantity and crystal form of microbially precipitated calcium carbonate. Sporosarcina pasteurii ATCC 11859 was used as the starting strain to obtain the mutant with high urease activity by atmospheric and room temperature plasma (ARTP) mutagenesis. Next, we investigated the optimal biomineralization conditions and precipitation crystal form using Plackett-Burman experimental design and response surface methodology (RSM). Biomineralization with 0.73 mol/l calcium chloride, 45 g/l urea, reaction temperature of 45℃, and reaction time of 22 h, significantly increased the amount of precipitated calcium carbonate, which was deposited in the form of calcite crystals. Finally, the repair of concrete using the optimized biomineralization process was evaluated. A comparison of water absorption and adhesion of concrete specimens before and after repairs showed that concrete cracks and surface defects could be efficiently repaired. This study provides a new method to engineer biocementing material for concrete repair.

• 대한한방부인과학회지
• /
• 제34권4호
• /
• pp.1-11
• /
• 2021

Objectives: Nephrotic syndrome is a kidney disorder, which is characterized by proteinuria, edema (swelling), and hyperlipidemia. Maydis Stigma (Corn silk) has been widely used in Asia as a traditional medicine and is known to have a diuretic effect and is used for the treatment of edema and indigestion. Methods: The aim of this study is to investigate the improvement effect of Maydis Stigma in treating nephrotic syndrome induced by puromycin aminonucleoside. Sprague-Dawley rats were intravenously injected with 75 mg/kg/day puromycin aminonucleoside, then treated with either Losartan or 200 mg/kg/day Maydis Stigma for seven days. Results: Maydis Stigma significantly decreased ascites and proteinuria level. Plasma levels of blood urea nitrogen (BUN) and plasma creatinine reduced significantly by Maydis Stigma. In addition, treatment with Maydis Stigma attenuated histological damage. Treatment with Maydis Stigma also restored podocin expression and reduced inflammation markers such as intracellular adhesion molecules (ICAM-1), monocyte chemotactic protein-1 (MCP-1), tumor necrosis factor alpha (TNF-α) and high-mobility group box-1 (HMGB1). Conclusions: Maydis Stigma ameliorates kidney injury in nephrotic syndrome rat models. Maydis Stigma exerts a renoprotective effect owing to its anti-inflammatory effects and reductions of ascites and proteinuria. Thus, these results indicate that Maydis Stigma is likely to be a promising agent in the treatment of nephrotic syndrome.

• Tribology and Lubricants
• /
• 제37권3호
• /
• pp.83-90
• /
• 2021

In this study, an ultrasonic nanocrystal surface modification (UNSM) was used to improve the mechanical properties, scratch resistance and tribological performance of Cu-based bimetals, which are usually used to manufacture sliding bearings and bushings for internal combustion engines (ICEs). Two different Cu-based bimetals, namely CuPb10Sn10 and CuSn10Bi7, were sintered onto a low carbon steel substrate. The mechanical properties and dry tribological performance using a tensile tester and micro-tribo tester were evaluated, respectively. The scratch resistance was assessed using a micro-scratch tester at an incremental load. The tensile test results showed that the yield strength (YS) and ultimate tensile strength (UTS) of both Cu-based bimetals increased after UNSM. Furthermore, the scratch and tribological tests results revealed that the scratch resistance and tribological performance of both Cu-based bimetals were improved by the application of UNSM. These improvements were mainly attributed to the eliminated pores, increased hardness and reduced roughness after UNSM. CuSn10Bi7 demonstrated better mechanical properties, scratch resistance and tribological performance than CuPb10Sn10. It was found that the presence of Bi in CuSn10Bi7 formed a Cu₁₁Bi₇ intermetallic phase, which is harder than Cu₃Sn. Hence, CuSn10Bi7 demonstrated higher strength and wear resistance than CuPb10Sn10. In addition, a CuSn10Bi7 formed both SnO₂ and Bi₂O₃ that prevented adhesion and improved the tribological performance. It can be expected that under dry tribological conditions, ICEs can utilize UNSM bearings and bushings made of CuSn10Bi7 instead of CuPb10Sn10 under oil-lubricated conditions.

• Journal of Electrochemical Science and Technology
• /
• 제12권3호
• /
• pp.330-338
• /
• 2021

An integral electrode-bipolar plate assembly, which is composed of electrode, conductive adhesive film (CAF) and bipolar plate, has been developed and evaluated for application with a vanadium redox flow battery (VRB) to decrease contact resistance between electrode and bipolar plate. The CAF, made of EVA (ethylene-vinyl-acetate) material with carbon black or CNT (Carbon Nano Tube), is applied between the electrode and the bipolar plate to enable an integral assembly by adhesion. In order to evaluate the integral assembly of VRB by adhesive film, the resistivity of integral assembly and the performance of single cell were investigated. Thus, it was verified that the integral assembly is applicable to redox flow battery. Through resistance and contact resistance of bare EVA and CAF films on bipolar plate were changed. Among the adhesive films, CAF film coated with carbon black showed the lowest value in through resistance, and CAF film coated with CNT showed the lowest value in contact resistance, respectively. The efficiency of VRB single cell was improved by applying CAF films coated with carbon black and CNT, resulting in the reduced overvoltage in charging process. Therefore, the energy efficiency of both CAF films, about 84%, were improved than that of blank cell, about 79.5 % under current density at 40 mA cm^-2. The energy efficiency of the two cells were similar, but carbon black coated CAF improved the coulomb efficiency and CNT coated CAF improved the voltage efficiency, respectively.

• 한국건축시공학회지
• /
• 제21권4호
• /
• pp.377-386
• /
• 2021

본 연구는 지난 2019년 4월에 대한건축학회 기술표준(AIK-S-001-2019)으로 제정된 복합열화 및 구조체 거동이 동시에 작용하는 환경에서의 방수층 성능 시험방법에 있어 일부 표준 내용 중 현장 시공성 반영 부족 문제와 표준 시공 방법으로서 오류 부분 등이 지적되면서 이를 보완해야 한다는 관련 업계, 시공사, 발주자(건설사) 등에서 의견이 제안되었다. 이에 본 연구를 통하여 시험 항목인 구조물 거동대응성능 시험방법에 있어 현장 시공성, 시험 결과의 정량화, 기타 개선 사항 등을 고찰하였다. 고찰된 내용을 이용하여 표준(안)으로 제정 중인 (가칭)점착형 복합 방수시트에 포함되어 진행하고 있는 구조물거동대응성능 시험방법에 표준 규격으로서 활용 가능성과 현장 시공성 반영 문제 등의 개선 내용을 검토하였다. 또한, 검토된 내용을 반영하여 1차와 2차 성능평가를 실시하여 그 결과를 도출하였다. 이에 본 연구를 통하여 도출된 개선(안) 및 성능평가 결과를 토대로 (가칭) 점착형 복합방수시트 제정(안)의 기초 자료로 활용하고자 한다.

• 박물관보존과학
• /
• 제27권
• /
• pp.91-102
• /
• 2022

철기 문화재 복원에 사용되고 있는 실리카 계열의 무기질 충전제는 열팽창률감소, 흐름성 개선 등의 기능성 부여를 목적으로 사용되고 있다. 그러나 충전제의 양이 많을 수록 복원재의 물성저하 및 황변 현상 촉진의 원인이되며, 결과적으로 재처리로 이어져 유물의 피로도를 상승시키는 결과를 초래한다. 따라서 복원제와 충전제의 혼합 정량화 및 황변성 연구 필요성이 강조될 수 밖에 없다. 이를 보완하기 위해 실리카계 경량 충전제인 Micro Shell을 비교군으로 물성연구를 진행하였다. 실험 결과 기존에 사용하던 충전제에 비해 Micro Shell이 황변도 발생 수치가 최대 34% 낮았으며, 충전제의 배합양에 따라서 공통적으로 접착력과 비중의 물성 수치값이 좋은 것을 확인하였다. 본 연구 결과 Micro Shell의 사용 가능성에 대해 확인하였다.

• 대한본초학회지
• /
• 제30권2호
• /
• pp.11-18
• /
• 2015

Objectives : Polygoni Multiflori Radix (Jeokhasuo in Korean) is a Oriental traditional herbs widely used in East Asian countries. Overconsumption of fructose results in hypertension, dyslipidemia, obesity and impaired glucose tolerance which have documented as a risk of cardiovascular diseases. This experimental study was designed to investigate the beneficial effects of an ethanol extract from Polygoni Multiflori Radix (PMR) in high-fructose (HF) diet-induced metabolic syndrome rat model. Methods : Sprague-Dawley (SD) rats were divided into three groups; Control group, receiving regular diet and tap water, HF group, and HF + PMR group both receiving supplemented with 65% fructose (n=10), respectively. The HF + PMR group initially received HF diet with PMR (100 mg/kg/day) for 8 weeks. Results : PMR significantly prevented the metabolic disturbances such as hyperlipidemia, hypertension and impaired glucose tolerance. Chronic treatment with PMR significantly decreased body weight, fat weight and adipocyte size, suggesting a role of anti-obesity effect. PMR led to improve the hyperlipidemia through the increase in HDL cholesterol level as well as the decrease in triglyceride and LDL cholesterol level. In addition, PMR suppressed adhesion molecules and endothelin-1 (ET-1) expression in aorta resulting in the decrease of hypertension. In muscle tissue, PMR significantly recovered the HF-induced insulin resistance through increase of insulin receptor substrate-1 (IRS-1), p-$AMPK{\alpha}1/2$, and p-Akt expression. PMR improved HF-induced metabolic disorders and its action was caused by energy metabolism-mediated insulin signaling activation. Conclusions : These results demonstrate that PMR may be a beneficial therapeutic for metabolic syndrome through the improvement of hyperlipidemia, obesity, insulin resistance and hypertension.

• 전기화학회지
• /
• 제25권1호
• /
• pp.32-41
• /
• 2022

Various steps in the electrode production process, such as slurry mixing, slurry coating, drying, and calendaring, directly affect the quality and, consequently, mechanical properties and electrochemical performance of electrodes. Herein, a new method of slurry coating is developed: Double-coated electrode. Contrary to single-coated electrode, the cathode is prepared by double coating, wherein each coat is of half the total loading mass of the single-coated electrode. Each coat is dried and calendared. It is found that the double-coated electrode possesses more uniform pore distribution and higher electrode density and allows lesser extent of particle segregation than the single-coated electrode. Consequently, the double-coated electrode exhibits higher adhesion strength (74.7 N m^-1) than the single-coated electrode (57.8 N m^-1). Moreover, the double-coated electrode exhibits lower electric resistance (0.152 Ω cm^-2) than the single-coated electrode (0.177 Ω cm^-2). Compared to the single-coated electrode, the double-coated electrode displays higher electrochemical performance by exhibiting better rate capability, especially at higher C rates, and higher long-term cycling performance. Despite its simplicity, the proposed method allows effective electrode preparation by facilitating high electrochemical performance and is applicable for the large-scale production of high-energy-density electrodes.

• Animal Bioscience
• /
• 제36권8호
• /
• pp.1209-1220
• /
• 2023

Objective: The use of probiotics as an alternative to antibiotics in animal feed has received considerable attention in recent decades. Lactic acid bacteria (LAB) have remarkable functional properties promoting host health and are major microorganisms for probiotic purposes. The aim of this study was to characterize LAB strains of the chicken digestive tract and to determine their functional properties for further use as potential probiotics in poultry. Methods: A total of 2,000 colonies were isolated from the ileum and cecal contents of the chickens based on their phenotypic profiles and followed by a preliminary detection for acid and bile tolerance. The selected 200 LAB isolates with exhibited well-tolerance in acid and bile conditions were then identified by sequencing the 16S rDNA gene, followed by acid and bile tolerance, antimicrobial activity, adhesion to epithelial cells and additional characteristics on the removal of cholesterol. Then, the two probiotic strains (L. ingluviei and L. salivarious) which showed the greatest advantage in vitro testing were selected to assess their efficacy in broiler chickens. Results: It was found that 200 LAB isolates that complied with all measurement criteria belonged to five strains, including L. acidophilus (63 colonies), L. ingluviei (2 colonies), L. reuteri (58 colonies), L. salivarius (72 colonies), and L. saerimneri (5 colonies). We found that the L. ingluviei and L. salivarius can increase the population of LAB and Bifidobacterium spp. while reducing Enterobacteria spp. and Escherichia coli in the cecal content of chickens. Additionally, increased concentrations of valeric acid and short chain fatty acids were also observed. Conclusion: This study indicates that all five Lactobacillus strains isolated from gut contents of chickens are safe and possess probiotic properties, especially L. ingluviei and L. salivarius. Future studies should evaluate the potential for growth improvement in broilers.

• 한국건축시공학회지
• /
• 제24권1호
• /
• pp.23-34
• /
• 2024

본 연구의 목적은 RC 구조물의 균열 보수용 폴리머 시멘트 복합체의 휨접착성능을 평가하기 위함이다. 시멘트 모르타르에 다양한 크기의 균열을 만들어 PCCs를 충전한 후, 소정의 양생을 거쳐 휨접착강도와 휨강도 개선 정도를 파악하였다. 연구결과, 균열보수용 PCCs의 휨접착강도는 시멘트의 종류, 폴리머 종류 및 폴리머 결합재비에 따라 영향을 받으며 실리카퓸을 혼입한 초조강시멘트를 사용한 경우가 보통시멘트를 사용한 경우에 비해 최대 19.0%의 휨접착강도 개선효과가 나타났다. PCCs로 충전된 시멘트 모르타르의 휨강도 개선 정도는 균열폭의 영향보다는 균열깊이에 따라 큰 차이를 보였으며 균열에 충전된 PCCs가 단순히 보수재료로서 기능뿐만 아니라 어느 정도 휨강도를 개선시키는 보강재료로서도 역할을 하였다. 본 연구결과, 초조강시멘트, 3종류의 폴리머 디스퍼전과 실리카퓸, 60%~80%의 높은 폴리머 결합재비로 제작한 PCCs가 균열을 충전하는 보수재료로서 현장에서 충분히 사용될 수 있을 것이다.