• Journal of Physiology & Pathology in Korean Medicine
• /
• v.25 no.3
• /
• pp.445-450
• /
• 2011

In order to validate the use of Olibanum as an anti-inflammatory drug in the traditional Korean medicine, I have investigated the effect of water-soluble extract of Olibanum (EO) on the expression of pro-inflammatory vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs) stimulated with tumor necrosis factor-${\alpha}$. The extract inhibited dose-dependently VCAM-1 expression without its cytotoxic effect on HUVECs, as measured by a flow cytometer using fluorescence-enhanced anti-VCAM-1 antibody, and significantly decreased mRNA levels of VCAM-1, as determined using reverse transcription polymerase chain reaction. These results suggest that Olibanum may have therapeutic potential in the control of endothelial disorders caused by inflammation.

• Journal of the Korean institute of surface engineering
• /
• v.32 no.4
• /
• pp.496-502
• /
• 1999

Electroplating of Ag and Au on the functional area of lead frames are required for good bonding ability in IC packaging. As the patterns of the lead frame become finer, development of new deposition technology has been required for solving problems associated with process control for uniform thickness on selected area. Sputtering was employed to investigate the adhesion between substrate Alloy42 and Ag film as a new candidate process alternative to conventional electroplating. Coating thickness of Ag film was controlled to 3.5$\mu\textrm{m}$ at room temperature as a reference. The deposition of film was optimized to ensure the adhesion by process parameters of substrate heating temperature at $100~300^{\circ}C$, sputter etching time at -300V for 10~30min, bias voltage of -100~-500V, and existence of Cr interlayer film of $500\AA$. The critical $load L_{c}$ /, defined as the minimum load at which initial damage occurs, was the highest up to 29N at bias voltage of -500V by scratch test. AFM surface image and AES depth profile were investigated to analyze the interface. The effect of bias voltage in sputtering was to improve the surface roughness and remove the oxide on Alloy42.

• International Journal of Oral Biology
• /
• v.43 no.1
• /
• pp.37-42
• /
• 2018

It is noted that Streptococcus mutans (S. mutans) triggers dental caries establishment by two major factors: the synthesis of organic acids, which demineralize dental enamel, and the synthesis of glucans, which mediate the attachment of bacteria to the tooth surface. Therefore, it is noted that the development of a more effective, substantial and safe preventive agent that works against dental caries and periodontal disease is required at this time. For this reason, the present study was designed to investigate the effect of croton seed ethanol extracts on the growth, acid production, adhesion, and insoluble glucan synthesis of S. mutans. In this case, the ethanol extract of croton seed showed concentration dependent inhibitory activity against the growth, acid production and adhesion of S. mutans. Especially, it is important to note that it has produced significant inhibition at the concentration of 0.1 and 0.2 mg/ml as compared to the control group. Moreover, these results suggest that the application of croton seed extract may be considered to be a useful method for the prevention of dental caries.

• Molecules and Cells
• /
• v.26 no.4
• /
• pp.329-337
• /
• 2008

Src-family kinases are critically involved in the control of cytoskeleton organization and in the generation of integrin-dependent signaling responses, inducing tyrosine phosphorylation of many signaling and cytoskeletal proteins. Activity of the Src family of tyrosine kinases is tightly controlled by inhibitory phosphorylation of a carboxy-terminal tyrosine residue, inducing an inactive conformation through binding with its SH2 domain. Dephosphorylation of C-ter tyrosine, as well as its deletion of substitution with phenylalanine in oncogenic Src kinases, leads to autophosphorylation at a tyrosine in the activation loop, thereby leading to enhanced Src activity. Beside this phophorylation/dephosphorylation circuitry, cysteine oxidation has been recently reported as a further mechanism of enzyme activation. Mounting evidence describes Src activation via its redox regulation as a key outcome in several circumstances, including growth factor and cytokines signaling, integrin-mediated cell adhesion and motility, membrane receptor cross-talk as well in cell transformation and tumor progression. Among the plethora of data involving Src kinase in physiological and pathophysiological processes, this review will give emphasis to the redox component of the regulation of this master kinase.

• Proceedings of the Korean Society of Propulsion Engineers Conference
• /
• 2004.10a
• /
• pp.215-222
• /
• 2004

We synthesized a aziridine bonding agent that play an important role in increasing the adhesion property between polymer binders and solid particles. It increases the physical properties of polymer-based propellant. Chemical reaction between two materials increase the interface adhesion. We analyzed and studied the chemical characteristics of the material and physical properties of a liner applied the aziridine bonding agent. We also studied the adhesion property of a liner/propellant applied the aziridine bonding agent. 3M was use as control group. In accordance with US MIS (Missile Interim Specification) we examined the chemical properties. As a result of our study, newly synthesized aziridine bonding agent was as good as quality of 3M HX-868.

• Journal of Adhesion and Interface
• /
• v.20 no.2
• /
• pp.71-75
• /
• 2019

Tannic acid is one of the most commonly found polyphenols in the vegetable field. Initially, research on tannins concentrated on physiological functions such as antioxidants. Recently, however, tannic acid has attracted much interest as a molecular glue as it has been found to interact virtually all bio-macromolecules such as proteins and DNA. The various properties of tannic acid are expected to control the wettability of the surface, contribute to energy storage and generation, and show potential as a medical agent. Here, tannic acid will be discussed about the interaction of with bio-macromolecules as a molecular glue, surface modification, and utilization of itself as biomaterials.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.7 no.6
• /
• pp.1064-1071
• /
• 2006

In electric motor coaches, the rolling stocks move by the adhesive effort between rail and driving wheel. Generally, the adhesive effort is defined by the function of both the weight of electric motor coach and the adhesive effort between rails and driving wheel. The characteristics of adhesive effort is strongly affected by the conditions between rails and driving wheel. When the adhesive effort decreases suddenly, the electric motor coach has slip phenomena. This paper proposes a re-adhesion control based on disturbance observer and sensor-less vector control. The numerical simulation and experimental results point out that the proposed readhesion control system has the desired driving wheel torque response for the tested bogie system of electric coach. Based on this estimated adhesive effort, the re-adhesion control is performed to obtain the maximum transfer of the tractive effort.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.31 no.4
• /
• pp.547-554
• /
• 2004

The surface characteristics of restoration such as surface roughness and droplet contact angle are important part for the process of bacterial adhesion. The purpose of this study is to compare plaque adhesion by measuring roughness, droplet contact angle, and amount of accumulated plaque on the surfaces of composite resins. Four kinds of composite resins, Z-100(Z1), Durafil(DF), Filtek supreme(FS), Clearfil AP X(CA) were used. Ten samples were divided into unpolished and polished group. Surface roughnesses and droplet contact angles were measured by profilometer and goniometer. Plaque weight gains are measured. The results were as follows: 1. The experimental group were rougher than the control group. Surface roughnesses were decreased in the following order; (Z1, DF, CA)>FS in the control group, and CA>Z1>(FS, DF) in the experimental group(P<0.05). 2 The control group showed larger contact angle than the experimental group. Contact angles were decreased in the following order; CA>(FS, DF, Z1) in the control group, and (CA, DF)>(FS, Z1) in the experimental group(P<0.05). 3. The experimental group showed more much plaque than the control group. The amounts of plaque accumulation in vitro were decreased in the following order; Z1>(DF, FS)>CA in the control group, and Z1>FS>(CA, DF) in the experimental group. The latter showed more much plaque than the former(P<0.05). 4. There were stronger correlation between plaque deposition and contact angle (P<0.05) than that of plaque deposition and surface roughness.

• Asian Pacific Journal of Cancer Prevention
• /
• v.13 no.9
• /
• pp.4435-4439
• /
• 2012

Purpose: This study aimed to explore the role of the Twist gene in the epithelial-mesenchymal transition of ovarian cancer. Methods: An RNA interference plasmid expressing a small interfering RNA (siRNA)-targeting Twist (Twist siRNA vector) was designed, constructed, and transfected into the human ovarian cancer cell line A2780. Transfection efficiency was assessed under a fluorescence microscope. Changes in the expression of Twist mRNA in A2780 after transfection with the pGenesil Twist shRNA plasmid were analyzed through RT-PCR. MTT assays and adhesion experiments were applied to determine changes in proliferation and adhesion ability of A2870 after transfection with the Twist shRNA plasmid. Changes in the expression of the E-cadherin and N-cadherin proteins in A2780 after transfection with the Twist shRNA plasmid were analyzed using Western blotting. Result: The restructuring plasmid pGenesil-Twist shRNA was constructed successfully. After 48 h of culture, 80% of the cells expressed high-intensity GFP fluorescence and stability. The expression of Twist decreased significantly after the transfection of the Twist shRNA plasmid (P<0.05). Proliferation of the transfected Twist shRNA cells showed no difference with that of the A2780-nontransfection or A2780-si-control groups (P>0.05) but the adhesion ability of A2780 decreased dramatically (P<0.05). Expression of the E-cadherin protein increased, whereas that of the N-cadherin protein decreased compared with that in the A2780-nontransfection or A2780-si-control groups (P<0.05). Conclusion: Twist is essential for epithelial-mesenchymal transition, invasion, and metastasis of ovarian cancer.

• Archives of Plastic Surgery
• /
• v.35 no.3
• /
• pp.349-353
• /
• 2008

Purpose: There are 3 well-known surgical procedures to treat Pierre Robin sequence: tongue-lip adhesion, distraction osteogenesis of mandible, and tracheostomy. The classical tongue-lip adhesion is an effective way to keep airway. The tongue, however, becomes quite non-mobile and appears dormant until the patient could control upper airway and the adhesion can be maintained for prolonged period. Most of all, this procedure does not provide the correction of the micrognathia. Distraction osteogenesis is a good technique to correct micrognathia and to prevent tracheostomies in patients with Pierre Robin sequence. But airway keeping procedure is needed during the distraction period. The purpose of this study is to determine the usefulness of temporary tongue-lip traction during the initial period of mandibular distraction in Pierre Robin sequence patients with severe airway problems requiring operative procedure. Methods: It was a prospective study of 2 Pierre Robin sequence patients aged between 4 months and 6 months requiring surgical procedure to correct recurrent and severe pulmonary complications. Two patients underwent distraction osteogenesis of mandible. During the operation, deep one tension suture was performed to tract the tongue and lip. When the patient gained control of upper airway at the initial period of distraction and micrognathia was corrected, the traction suture was removed. Results: All patients were followed up. No patients complained severe pulmonary complications and tracheostomy could be avoided. No patients had severe pulmonary complication. The pulmonary condition of patients was good. Conclusion: In severe Pierre Robin sequence case, temporary tongue-lip traction is a good assistant method in distraction osteogenesis because this method can avoid tracheostomy.