• Environmental Analysis Health and Toxicology
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• 제13권1_2호
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• pp.33-41
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• 1998

Chlorpyrifos, o,o diethyl-o-(3,5,6-trichloro-2-pyridyl) phosphorothioate, is a broad spectrum organophosphate insecticide. The use of chlorpyrifos has been increased more and more as pesticide. But the effects of chlorpyrifos on the immune alterations has not been yet observed. Therefore, we investigated the effects of chlorpyrifos on the immune alterations in CICA male mice. Chlorpyrifos was administered to mice by a single intraperitoneal injection for the purpose of observing acute effects. On the one hand to get the information on immunopathologic alterations we observed hematological values, counted total circulating leukocytes and assessed the ratio of lymphocytes and neutrophils from the peripheral blood, measured the ratio of organ/body weight and counted splenic cellularity in CBA male mice which treated chlorpyrifos intraperitoneally. But we could not find any significant immunopathologic alterations statistically by a single intraperitoneal injection. Also, the exposure of chlorpyrifos caused no significant change in the number of PFC/10$^6$ spleen cells at any three given doses. On the other hand a singte intraperitoneal injection of chlorpyrifos decreased the lymphocyte proliferation response slightly to ConA or LPS stimulation at a dose of 6 mg/kg b.w. Administrations of chlorpyrifos reduced mixed leukocyte response(MLR). MLR was decreased moderately at doses of 3mg/kg b.w. and 6mg/kg b.w. Therefore, all these findings suggest that chlorpyrifos may alter the immune functions acutely. especially by the changes of T lymphocyte activity.

• Journal of Acupuncture Research
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• 제28권4호
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• pp.9-18
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• 2011

Objectives : This study was performed to evaluate the effects of the acupuncture therapy in combination with Soyeom pharmacopuncture therapy on acute whiplash injury by traffic accident. And thermal change of posterior neck and shoulder was observed. Methods : The clinical study was conducted to 20 cases of acute whiplash injury patients who had been admitted in Dept. of Acupuncture and Moxibusion, Woosuk University Hospital from December 1, 2010 to May 31, 2011. The patients were randomly divided into two groups. In one group(test group), patients were treated with the acupuncture therapy in combination with Soyeom pharmacopuncture therapy. And in the other group(control group), patients were treated with acupuncture therapy only. To estimate the efficacy of treatments, visual analog scale(VAS), neck disability index(NDI) of posterior neck pain were checked. And also thermal change of posterior neck and shoulder was observed by digital infrared thermographic imaging(DITI). Results : Both group represented effective improvement in VAS and NDI of posterior neck pain. Especially, test group is more effective than control group in VAS comparison, statistically. In test group, temperature of posterior neck and shoulder was decreased after treatments. But there was no significant difference between two groups on thermographic change. Conclusions : Through this study, Soyeom pharmacopuncture therapy is considered to be effective for reducing pain in acute whiplash injury patients. And after Soyeom pharmacopuncture therapy, temperature of posterior neck and shoulder show a tendency to decrease.

• 대한임상전기생리학회지
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• 제5권2호
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• pp.23-33
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• 2007

Summary of background data: At the wound from life of the human being very regarding at and a healing process the wound in the portion which is important meantime the many research is accomplished and healing process at the wound the research is small very from the physical therapy territory. Purpose: This study was performed to examine the effects of cold therapy and pulsed ultrasound treatment in the process of acute wound healing. To this end, we measured changes in the length of the wounds, and observed tissues through an optical microscope in order to evaluate the healing process of the acute wounds. Methods: We divided twenty Sprague-Dawley rats into four experimental groups of five rats each and treated them for three days after wound creation. Then we extracted tissues from the wounds on day 6th after wound creation and then took them out for hematoxylin-eosin(HE) staining. We measured changes in the length of the wounds every other day. Result: We were able to detect significant statistical differences in the cold therapy and pulsed ultrasound-treated wounds compared with control wounds. We observed tissues through an optical microscope and found the tissues in cold therapy and pulsed ultrasound-treated wounds healed well. Conclusions: Overall results indicated that the use of cold therapy and pulsed ultrasound treatment were effective in the process of acute wound healing.

• Journal of Animal Science and Technology
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• 제58권11호
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• pp.39.1-39.12
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• 2016

Background: The current study investigates the anti-stress effects of clove (Eugenia caryophyllus) extracts (0, 200, 400, and 600 mg/kg) on serum antioxidant biomarkers, immune response, immunological organ growth index, and expression levels of acute phase proteins (APPs); ovotransferrin (OVT), ceruloplasmin (CP), ceruloplasmin (AGP), C-reactive protein (CRP), and serum amyloid-A (SAA) mRNA in the immunological organs of 63-d-old male black-meated Silkie fowls subjected to 21 d chronic heat stress at $35{\pm}2^{\circ}C$. Results: The results demonstrated that clove extract supplementation in the diet of Silkie fowls subjected to elevated temperature (ET) improve growth performance, immune responses, and suppressed the activities of glutathion peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), and thioredoxin reductase (TXNRD); reduced serum malonaldehyde (MDA) and glutathione (GSH) concentrations when compared with fowls raised under thermoneutral condition (TC). Upon chronic heat stress and supplementation of clove extracts, the Silkie fowls showed a linear increase in GSH-Px, SOD, CAT, and TXNRD activities (P = 0.01) compared with fowls fed diets without clove extract. ET decreased (P < 0.05) the growth index of the liver, spleen, bursa of Fabricius and thymus. However, the growth index of the liver, spleen, bursa of Fabricius and thymus increased significantly (P < 0.05) which corresponded to an increase in clove supplemented levels. The expression of OVT, CP, AGP, CRP, and SAA mRNA in the liver, spleen, bursa of Fabricius and thymus were elevated (P < 0.01) by ET compared with those maintained at TC. Nevertheless, clove mitigates heat stress-induced overexpression of OVT, CP, AGP, CRP and SAA mRNA in the immune organs of fowls fed 400 mg clove/kg compared to other groups. Conclusions: The results showed that clove extracts supplementation decreased oxidative stress in the heat-stressed black-meated fowls by alleviating negative effects of heat stress via improvement in growth performance, antioxidant defense mechanisms, immunity, and regulate the expression of acute phase genes in the liver and immunological organs.

• Environmental Analysis Health and Toxicology
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• 제25권2호
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• pp.153-159
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• 2010

Perfluorooctane sulfonic acid (PFOS) and Perfluorooctanoic acid (PFOA) are the principal chemicals known as perfluoroalkyl acids (PFAs). Despite the widespread use of these compounds, relatively little is known about their fate and effects. The purpose of this study was to determine the toxic effects of PFOS and PFOA on Daphnia magna. In the acute toxicity test, D. magna were exposed for 48 hours at concentrations of 0, 30, 45, 67.5, 101.25 and 151.88 mg/L PFOS, and 0, 100, 160, 225, 337.5 and 506.25 mg/L PFOA, respectively. In the case of chronic toxicity test, D. magna were exposed through water for 21 days at concentrations of 0, 0.375, 0.75, 1.5, 3 and 6 mg/L PFOS, and 0, 1.25, 2.5, 5, 10 and 20 mg/L PFOA, respectively. Acute toxicity was assessed on the basis of immobility, while chronic toxicity was assessed on the basis of fecundity. The acute toxicity test on PFOS and PFOA showed that the values of $EC_{50}$ were 50.90 mg/L and 253.47 mg/L, respectively. In the chronic test, fecundity was reduced significantly at 1.5 mg/L of PFOS and 10 mg/L of PFOA, respectively. These results indicated that PFOS is more toxic to zooplankton than PFOA, and both chemicals have some hazard demonstrates risk for acute or chronic toxicity to freshwater organism.

• 동서간호학연구지
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• 제23권2호
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• pp.115-123
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• 2017

Purpose: The purpose of this study was to determine the effects of teaching communication training using $Gagn{\acute{e}}^{\prime}s$ 9 events of instruction and video clip on communication competency and interpersonal relations of nursing students. Methods: The participants were 79 nursing students (41 in the intervention group, 38 in the control group). A nonequivalent control group design was used. The research was carried out from September 7 to November 20, 2015. Teaching communication skills using $Gagn{\acute{e}}^{\prime}s$ 9 events of instruction and video clip were provided to the intervention group 180 minutes/session once a week for 9 weeks. Data were analyzed using chi-square test, independent t-test and ANCOVA with SPSS/PC version 21.0. Results: The intervention group reported significantly higher scores of communication competency(F=8.41, p=.005) and interpersonal relations(F=8.97 p=.004) compared to those of the control group at the completion of the intervention. Conclusion: The findings of this study show that $Gagn{\acute{e}}^{\prime}s$ 9 events of instruction and video clip is an effective communication training for improving communication competency and interpersonal relations in nursing students. A randomized clinical trial is needed to confirm the value of communication training using $Gagn{\acute{e}}^{\prime}s$ 9 events of instruction and video clip for nursing students.

• 한국식품영양과학회지
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• 제28권6호
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• pp.1355-1363
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• 1999

The purpose of this study was to investigate the effects of vitamin E on liver microsomal cytochrome P450 contents and xanthine oxidase activity in acute cadmium poisoned rats. Sprague Dawley male rats weighing 100$\pm$10g were randomly assigned to one normal and three cadmium injected groups. Cadmium injected groups were fed vitamin E free diet(0E Cd group), 40mg vitamin E per kg diet(40E Cd group) or 400mg vitamin E per kg diet(400E Cd group). Vitamin E level of normal group was 40mg per kg diet. Animals were injected intraperitoneally with 2.0mg Cd2+/kg bw for 4 days after the rats were fed diets with three different levels of vitamin E for 2 and 4 weeks. Body weight, food intake and feed efficiency ratio of cadmium injected animals, were decreased compared with those of normal group. The weights of liver and kidney in cadmium injected groups were not different from those of normal group. Cadmium contents of liver in cadmium groups were 160 fold higher those that of normal group. Accumulation of cadmium poisoned rat liver was reduced by vitamin E supplementation. Contents of blood hemoglobin and hematocrit in 0E Cd groups were decreased to 2~ 14% of those of the other groups. Contents of serum triglyceride in all experimental groups were not significantly different each other. Levels of serum total cholesterol, LDL cholesterol and antherogenic index in 0E Cd and 40E Cd groups were higher than those of normal group, while the contents of HDL cholesterol in 0E Cd and 40E Cd groups were lower than those of normal group. Xanthine oxidase(XOD) activity and cytochrome P450 contents in the liver were significantly increased in cadmium injected groups but these were reduced by vitamin E supplementations. The present results indicate that acute cadmium poisoning in rats causes increasing free radical generation systems in the liver and that leads to liver tissue damage. But these abnormalities can be reduced by dietary vitamin E supplementations.

• 대한의생명과학회지
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• 제19권1호
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• pp.70-78
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• 2013

This study was conducted to identify the effects of intake of beta-blocker and aerobic exercise on heart rate recovery (HRR) and exercise capacity after maximal exercise in acute coronary syndrome patients (ACS). Subjects were divided into 4 groups; Beta-blocker-Exercise group (n=10), Beta-blocker group (n=10), Exercise group (n=10), Control group (n=10). Symptom-limited grade exercise test were conducted on subjects pre- and post- 6 week study, to measure maximal heart rate, heart rate at 1 minute and 2 minutes at the recovery stage, maximal oxygen uptake and maximal exercise time. Study resulted in significant improvements in heart rate recovery for BB-Ex group ($17.40{\pm}5.58$ bpm to $26.10{\pm}6.66$ bpm), maximal oxygen uptake for BB-Ex group ($30.46{\pm}8.63$ to $36.33{\pm}8.10$) and Ex group ($29.04{\pm}4.93$ to $34.31{\pm}5.19$), and total exercise time for BB-Ex group ($906.30{\pm}158.57$ to $1018.50{\pm}151.21$), BB group ($805.70{\pm}182.94$ to $897.50{\pm}160.16$) and Ex group ($870.00{\pm}145.63$ to $961.90{\pm}107.29$). Therefore it showed the biggest improvement for heart rate recovery and exercise capacity in acute coronary syndrome patients when both Beta-blocker and aerobic exercise were in effect.

• Physical Therapy Rehabilitation Science
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• 제8권4호
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• pp.187-193
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• 2019

Objective: The aim of this preliminary study was to investigate the effects of virtual reality combined with balance training on upper limb function, balance, and activities of daily living (ADL) in persons with acute stroke. Design: Randomized controlled trial. Methods: Fourteen acute stroke survivors were recruited and randomly assigned into two groups: the experimental group (n=7) and the control group (n=7). Both groups performed the conventional rehabilitation therapy for 30 minutes a day, 5 times a week, for 4 weeks. Additionally, the experimental group conducted the virtual reality training for 30 minutes on an unstable surface during each session, whereas the control group performed balance training for 30 minutes on a stable surface. All measurements were performed before and one day after intervention. Upper limb function, balance, and ADL were assessed using the Manual Function Test (MFT), the Berg Balance Scale (BBS), and the Korean version of the Modified Barthel Index (K-MBI), respectively. Results: Both groups showed significant improvements in MFT, BBS, K-MBI after intervention (p<0.05). There were no significant differences between the experimental and control groups with respect to MFT, BBS, and K-MBI after intervention. The experimental group showed a greater decrease in fall risk (BBS<45) after intervention than the control group (p<0.05). Conclusions: These findings suggest that virtual reality combined with balance training has a better effect on balance improvement than virtual reality training alone in persons with acute stroke.

• PNF and Movement
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• 제15권3호
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• pp.267-279
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• 2017

Purpose: The aim of the investigation was to identify the effects of purposeful activities combined with music on upper extremity function, motivation, and mood in acute stroke patients. Methods: For the study, 19 acute stroke inpatients were recruited at a general hospital in Korea. The patients were allocated between an experimental group (n=9) and a control group (n=10). The experimental group performed purposeful activities with their preferred music, and the control group carried out purposeful activities without music. The 2 groups received the treatments 30 min per day, 5 days per week, for 4 weeks. Measurements were taken before and after intervention, including a manual function test (MFT) for measuring upper extremity function, a volitional questionnaire (VQ) for assessing motivation, and the Beck depression inventory (BDI) and Beck anxiety inventory (BAI) for assessing mood. Results: In post-treatment measurement, both groups showed greater MFT, VQ, and BDI scores than in pre-treatment. Significantly greater VQ and BDI changes were represented in the experimental group as compared to the control group. Conclusion: Based on the findings of this study, purposeful activities combined with music have a positive effect on motivation and depression in acute stroke patients.