• Molecular & Cellular Toxicology
• /
• 제5권4호
• /
• pp.273-282
• /
• 2009

Cadmium (Cd) and tributyltin (TBT) are common contaminants of marine and freshwater ecosystems, and can induce the formation of reactive oxygen species (ROS). These ROS can, in turn, cause oxidative stress. In the present study, we investigated time-related effects of Cd (0.05 and 0.1 ppm) and TBT (5 and 10 ppb) treatment on antioxidant enzyme activity, i.e., the activity of superoxide dismutase (SOD) and catalase (CAT) in the gills and digestive glands of the ark shell, Scapharca broughtonii. In addition, hydrogen peroxide ($H_2O_2$) concentrations, lysozyme activity, and glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) levels were measured in the hemolymph. We found that Cd and TBT treatment significantly increased antioxidant enzyme mRNA expression and activity in the digestive glands and gills in a time-dependent manner. In response to the Cd and TBT treatments, antioxidant enzymes mRNA expression and activity increased up to day 5 in the digestive glands and then decreased by day 7. In the gills, antioxidant enzymes mRNA expression and activity increased up to day 3 and then decreased by day 5. Likewise, $H_2O_2$ concentrations significantly increased up to day 5 and then decreased by day 7. Finally, lysozyme activity decreased during the experimental period, whereas GOT and GPT levels were significantly increased in a time-dependent manner. These results suggest that antioxidant enzymes play an important role in decreasing ROS levels and oxidative stress in ark shells exposed to Cd and TBT.

• Journal of the Korean Wood Science and Technology
• /
• 제45권6호
• /
• pp.809-827
• /
• 2017

Steam explosion was applied to extract phenolic compounds from oak wood (Quercus mongolica). The effects of three independent factors (ethanol concentration, extraction temperature and extraction time) on the total phenolic content, DPPH radical scavenging activity, and antimicrobial activity from the steam exploded oak wood were optimized using response surface methodology (RSM). The independent variables were coded at three levels and their actual values were selected on the basis of preliminary experimental results. The following optimal extraction conditions were selected: ethanol concentration 82.0%, extraction temperature $71.7^{\circ}C$, and extraction time 60.5 min for total phenolic content; ethanol concentration 78.3%, extraction temperature $70.3^{\circ}C$, and extraction time 57.6 min for DPPH radical scavenging activity; ethanol concentration 80.6%, extraction temperature $68.4^{\circ}C$, and extraction time 59.0 min for antimicrobial activity. The experimental values agreed with those were predicted within confidence intervals indicating the suitability of RSM in optimizing the ethanol extraction of phenolic compounds from the steam exploded oak wood. Under the optimized conditions, the experimental value of the total phenolic content was 111.8 mg GAE/g dry steam exploded oak wood, DPPH free radical scavenging activity was 65.7%, and antimicrobial activity was 17.0 mm, and those are reasonably close to the predicted values (109.2 mg GAE/g dry steam exploded oak wood, 62.3% and 15.9 mm, respectively).

• Food Science and Biotechnology
• /
• 제16권4호
• /
• pp.565-571
• /
• 2007

This study was carried out to investigate an optimum condition for the high angiotensin-l converting enzyme (ACE) inhibitory activity and the yield on enzyme concentration, casein concentration, and hydrolysis time. The optimum condition was performed by response surface methodology for acquirement of casein hydrolysate of milk which shows high ACE inhibitory activity, Among 8 tested enzymes, Protamex showed the highest activation degree with 77.03 unit/g from casein. Their hydrolysis degrees of flovourzyme 500MG, protamex, mixture from 1% casein were 85.5, 88.5, and 93.5%, respectively. The ranges of enzyme concentration (0.25-1.25%), casein concentration (2.5-12.5%), and hydrolysis time (20-100 min) as 3 independent variables through preliminary experiments of the yield of casein hydrolysate and ACE inhibitory activity, and it shows optimum response surface at a saddle point. It shows enzyme concentration (0.64%), casein concentration (8.38%), and hydrolysis time (55.81 min) in the yield aspect and showed the highest activity at enzyme concentration (0.86%), casein concentration (5.97%), and hydrolysis time (63.86 min) in ACE inhibitory aspect. The $R^2$ value of a fitted optimum formula on the hydrolysis yield was 0.9751 as the significant level of 1%. The $R^2$ value of a fitted optimum formula on ACE inhibitory activity is 0.8398, and the significance is recognized in the range of 5%.

• 대한물리의학회지
• /
• 제3권4호
• /
• pp.215-224
• /
• 2008

Purpose : The purpose of this study was to evaluate the effects of the Hahoe mask dance on the gait and muscle activity in the elderly who lived at a elderly welfare facility. Methods : Dancing group was consisted of 20 subjects(over 70 years). The time of measurement was assigned in pre measurement, after 1 month, after 2 months. Subjects was received the Hahoe mask dance for three times a week during 8 weeks. The control group was consisted of 20 the elderly (similar age) who didn't received dancing. Assessment of gait function included the gait velocity, step time, stride, step length, it was analyzed in activity for 4 muscles (rectus femoris, biceps femoris, tibialis anterior, and lateral gastrocnemius). Results : Activities for muscles of control group was decreased in rectus femoris, bieps femoris, however, those of experimental group was increased significantly(p<.05). While control group showed decrease in the gait velocity per second (p<.05), experimental group showed statistically significant increase(p<.05). Control group had a significant long time than that of control group in gait time of right and left foot(p<.05). Control group was a little longer than control group in stride time of right and left foot(p<.05). Step length for control group was shorter than that for experimental group both foots(p<.05). Conclusion : In conclusion, this study confirmed that activities for 4 muscles by EMG have showed significant increases, so we will use a programme of muscle improvement in elderly community welfare faculty. We recommend that further research should explore the degree of muscle activity by larger sample sizes and longer follow-up periods.

• 중환자간호학회지
• /
• 제6권2호
• /
• pp.65-77
• /
• 2013

Purpose: The purpose of the study was to analyze the nursing activities related to continuous renal replacement therapy (CRRT) in the intensive care units (ICU). Methods: We developed a list of 31 CRRT related nursing activities based on literature review and reviewed by expert group. A total of 109 direct time measurement records by 43 ICU nurses were collected and analyzed in terms of total time per shift, frequency, standard time, difficulty and performance levels of each CRRT nursing activity. Results: The mean time for CRRT nursing activity was 85.60 minutes per 8 hour shift. Nurses have spent average $9.46{\pm}6.98$ minutes in a shift for "waste fluid bag change" activity. In addition, "check catheter location" was the most time consuming single activity. The most difficult activity was "counseling-answer" and the most competent one was "dialysis solutions change". Conclusion: The CRRT nursing activities accounted for a significant portion of total nursing workload. Practical allocation of nursing staff for CRRT patient along with development of a new nursing cost system need to be considered. Continuous nursing educational and training programs on CRRT should be developed.

• 대한지역사회영양학회지
• /
• 제20권2호
• /
• pp.120-128
• /
• 2015

Objectives: The purpose of this study was to examine the relative impact of seasonal differences and age on dietary vitamin D intakes, outdoor activity time and serum vitamin D status among Korean postmenopausal women. Methods: A cross-sectional study was conducted with 164 free-living postmenopausal women (mean age=55.4 years) in Daegu, Korea. Dietary intake, daily physical activity patterns and fasting blood samples were collected during summer and winter. We compared the results from 75 women during summer and 89 women during winter. Dietary intake of vitamin D was assessed by 24-hour recall method. Daily outdoor activity time was derived from physical activity diary. Results: The average dietary intake of vitamin D of the participants was $3.7{\mu}g$ during summer, $3.3{\mu}g$ during winter, showing no significant difference between the two seasons. The average time spent on outdoor activities was not significantly different between summer ($=35.1{\pm}75.0min/d$) and winter ($=48.5{\pm}76.8min/d$). The average serum 25-(OH) vitamin D concentrations of participants was $17.5{\pm}7.5ng/mL$ in the summer and $13.4{\pm}4.3ng/mL$ in the winter, showing no significant differences by season. Dietary intake of vitamin D was not related to age. When total subjects were divided into two groups by age, the average serum 25-(OH) vitamin D concentration was significantly higher in older group (p=0.047) and time spent on outdoor activities was also (p=0.018) significantly higher in the older group. Conclusions: In order to improve the current vitamin D status of Korean postmenopausal women, nutrition education programs should focus more on adequate intake of vitamin D, while maintaining enough outdoor activities over the season.

• 대한본초학회지
• /
• 제26권4호
• /
• pp.139-147
• /
• 2011

Objectives : The aim of this study is to research an anti-thrombus effect by Diospyros Kaki Calyx. Methods : The healthy human plasma were gained and used in vitro study such as factor X activity (FXa) inhibition, prothrombinase inhibition, prothrombin time (PT) and activated partial thromboplastin time. Fifteen SD rats were divided into three groups ; intact control group (orally administrated with distilled water 5ml/kg) and two experimental group treated with extract of diospyros kaki calyx (EKC). Experimental rats were orally 600 mg/kg concentration of EKC and 200 mg/kg concentration of EKC. After an hour from administration, we anesthetized rats and made arteriovenous (AV) shunt rat models to study weight of thrombus, took whole blood to study content of thromboxane B2 and blood clotting time. Results : In vitro, EKC significantly increased inhibitory activity of FXa, prothrombinase compared with intact control group ($^*P$ <0.05). PT and aPTT were increased in EKC treated (600 mg/kg) group compared with intact control group ($^*P$ <0.05). In vivo, blood clotting time of experiment group treated with EKC 600 mg/kg were significantly increased compare with that of intact control group (p<0.05) and content of thromboxane B2 was significantly decreased in group treated with EKC 600 mg/kg in serum. The weight of thrombus were significantly reduced in group treated with EKC 600 mg/kg compared with intact control group (p<0.05). But in vivo experiment study, those parameters of group treated with EKC 200 mg/kg were relatively decreased compared with those of intact control group without statistical significance. Conclusions : EKC has an antithrombic activity because of inhibition internal course such as FXa and prothrombin. And EKC inhibited a hole blood clotting in vivo experiment by low content of thromboxane B2.

• 한국전문물리치료학회지
• /
• 제18권1호
• /
• pp.9-17
• /
• 2011

The purpose of this study was to assess visual biofeedback's influence on trunk muscles' (EMG) activity and endurance holding time for correct position during whole-body tilt exercise. For the study, we recruited 14 volunteers who showed no symptom of lumbar disease during medical tests. We measured the EMG activity of their rectus abdominis, external abdominal oblique, internal abdominal oblique and erector spinae muscles, and their endurance holding time for correct position during $40^{\circ}$ anterior and posterior whole-body tilt under two conditions: whole-body tilt with and without visual biofeedback. Resistance with gravitational force on the trunk during whole-body tilt was applied by using a device that had a monitor on which the subjects could check their alignment and that sounded an alarm if a subject's alignment collapsed. The study showed an increase in the EMG activity of external abdominal oblique, internal abdominal oblique/rectus abdominis ratio and endurance holding time for correct position during both $40^{\circ}$ anterior and posterior whole-body tilt with visual biofeedback compared with without visual biofeedback (p<.05). We suggest that the whole-body tilt exercise with visual biofeedback could be a beneficial strategy for selectively strengthening the internal abdominal oblique muscle and minimizing the rectus abdominis muscle's activity while maintaining correct alignment during whole-body tilt exercise.

• 대한본초학회지
• /
• 제32권1호
• /
• pp.33-40
• /
• 2017

Objectives : The objective of this study is to evaluate antioxidant activity of water extracts from steamed Polygonati Rhizoma(PR). Methods : The alcohol steamed PR from Jinju-si, Gyeongsangnam-do and Samcheok-si Gangwon-do are treated for the research into the antioxidant activity of the water extract. The sample was divided into 4 groups that PR without steaming process, PR with once, second and third steaming process. The antioxidant activities of polyphenol, flavonoid, DPPH, SOD, hydroxyl radical and nitrite scavenging activity. Results : The total content of polyphenol was the grow rapidly when the number of steaming adds up. For the third time, both the steamed PR from Jinju-si and the one from Samcheok-si in the water extract indicate the highest content levels measured $50.59\;mg/m{\ell}$ and $37.33\;mg/m{\ell}$ respectively. The more the number of steaming increases, the more rising the content of flavonoid. The steamed PR from Jinju-si and Samcheok-si in the third time are measured $30.03\;mg/m{\ell}$ and $54.9\;mg/m{\ell}$ respectively. DPPH radical scavenging activity of the steamed PR rises high rather than the non-steamed one when the number of steaming increases. As increase steaming time, SOD like activity and hydroxyl radical scavenging ability were increasing too. The steamed PR from Jinju-si in the third time is measured as the highest level 22.4%, 71.90% respectively. As increase steaming time, nitrite scavenging ability was the number was decreased. Conclusions : These results suggest that water extracts from steamed PR, exhibited higher antioxidant activities can be potentially used as proper natural antioxidants.

• 한방비만학회지
• /
• 제10권2호
• /
• pp.27-39
• /
• 2010

Objectives This study was performed to figure out relations of self-esteem, anxiety and the other factors related with obesity in obese children. Methods Forty-two children(21 boys and 21 girls) those who were diagnosed obesity have been recruited. To assess psychological factors, Self-esteem inventory(SEI), Revised Children's Manifest Anxiety Scale(RCMAS), International Physical Activity Questionnaires(IPAQ), Korean Eating Attitudes Test(KEAT-26) and Children's Depression Inventory(CDI) were administered. Behavioral characteristics were also analyzed. Results Self-esteem(SEI) had significant positive relationship with physical activity(IPAQ) and sleeping time. Self-esteem had significant negative relationship with anxiety(RCMAS), eating disorder(KEAT-26) and watching TV time. Anxiety had significant positive relationship with eating disorder(KEAT-26) and watching TV time, and negative relationship with physical activity(IPAQ) and sleeping time. All of participants seemed to be having depressive tendency. Conclusion Self-esteem and anxiety were significantly related. And eating disorder, physical activity, sleeping time and watching tv also affected to the self-esteem and anxiety of obese children.