• Molecules and Cells
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• v.45 no.3
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• pp.148-157
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• 2022

Hepatitis C virus (HCV) is a major cause of chronic liver disease and is highly dependent on cellular proteins for viral propagation. Using protein microarray analysis, we identified 90 cellular proteins as HCV nonstructural 5A (NS5A) interacting partners, and selected telomere length regulation protein (TEN1) for further study. TEN1 forms a heterotrimeric complex with CTC and STN1, which is essential for telomere protection and maintenance. Telomere length decreases in patients with active HCV, chronic liver disease, and hepatocellular carcinoma. However, the molecular mechanism of telomere length shortening in HCV-associated disease is largely unknown. In the present study, protein interactions between NS5A and TEN1 were confirmed by immunoprecipitation assays. Silencing of TEN1 reduced both viral RNA and protein expression levels of HCV, while ectopic expression of the siRNA-resistant TEN1 recovered the viral protein level, suggesting that TEN1 was specifically required for HCV propagation. Importantly, we found that TEN1 is re-localized from the nucleus to the cytoplasm in HCV-infected cells. These data suggest that HCV exploits TEN1 to promote viral propagation and that telomere protection is compromised in HCV-infected cells. Overall, our findings provide mechanistic insight into the telomere shortening in HCV-infected cells.

• The Korean Journal of Malacology
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• v.29 no.4
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• pp.313-323
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• 2013

Gonad development, the reproductive cycle, first sexual maturty and size at 50% of group sexual maturity (the biological minimum size) of Gomphina (Macridiscus) veneriformis were investigated for clams collected from the coastal waters of Donghae City, the East Sea of Korea by histological, and morphometric analysis. Monthly variations of the gonad index showed a pattern similar to that of the reproductive cycle. The reproductive cycle with the gonad developmental stages in female and male G. (M.) veneriformis can be classified into five successive stages: early active stage (December to March), late active stage (March to June), ripe stage (June to July), partially spawned stage (June to August), and spent / inactive stage (September to December). The spawning period continued from June to August, with a peak between July and August when the seawater temperature exceeds $20^{\circ}C$. The percentages of first sexual maturities of female and male clams ranging from 25.1 to 30.0 mm were 56.3% in females and 61.1% in males, and for clams over 30.1 mm shell length, it was 100%. Shell lengths at 50% of group sexual maturity (biological minimum size, $RM_{50}$) were 27.71 mm in females and 26.31 mm in males. Because harvesting clams < 26.31 mm in shell length could potentially cause a drastic reduction in recruitment, a measure indicating a prohibitory fishing size should be taken for adequate fisheries management.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.36 no.6
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• pp.673-679
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• 2012

Vehicle rollover is a serious kind of accident, particularly for sport utility vehicles, and its occurrence can be minimized by utilizing active rollover prevention systems. The performance of these protection systems is very sensitive to vehicle inertial parameters such as the vehicle's mass and center of mass. These parameters vary with the number of passengers and in different load situations. In this paper, a unified method for vehicle mass estimation is proposed that takes into account the available driving conditions. Three estimation algorithms are developed based on longitudinal, lateral, and vertical vehicle motion, respectively. Then, the three algorithms are combined to extract information on the vehicle's mass during arbitrary vehicle maneuvering. The performance of the proposed vehicle mass estimation method is demonstrated through real-time experiments.

• Korean Parent-Child Health Journal
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• v.5 no.2
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• pp.161-176
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• 2002

Among the causes of childhood behavioral problems - that is, physiological, psychological, and environmental factors -, the common element of non-organic psychological and environmental factors is influences from the parents. Roles of mother who does the biggest emotional exchange with the child after birth may have substantial association with the childhood behavioral problems. In this connection, the present explanatory study was conducted for the purpose of understanding the causal structural relationship in which mother's child caring behaviors affect child behavioral problems. The data collection period was July 2 - 7, 2001. The subject was 4th, 5th, 6th graders' mothers in 4 elementary schools located in Daejoen city and 299 surveys were used in the analysis. As for the tools used in this study to assess the mother's child caring Behavior Scale by Park, Seong-Yeon and Yi, Sook (1990) was used to measure the mother's behaviors. The child behavioral problems were measured with the Korean-Child Behavior Checklist (K-CBCL), standardized into the Korean version by O, Gyung-Ja, Yi, Hye-Ryon, Hong, Gang-Eui, and Ha, Eun-Hye. For the data processing, the frequency and the percentage were calculated, and the analyses of variance, correlation were carried out. The result of this study is summarized as follows: First, the examination of the effect of mother's general characteristics on the mother's caring behavior shows that significant differences are made by the active participation (p=0.020) in the occupation, affection(p=0.000), authority control(p=0.013), achievement(p=0.030), active participation(p=0.032) in the education level, consistency regulation(p=0.007) in the religion. Second, the examination of the effect of mother's caring behaviors on the child behavioral problems shows that significant differences are made by some child behavioral problems in the affection, achievement, consistency regulation, authority control, over protection caring behaviors. Third, as for the correlation between mother's child caring behavior and child behavioral problems, a significant correlation is revealed between the negative child caring behavior and child behavioral problems.

• Carbon letters
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• v.8 no.4
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• pp.280-284
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• 2007

Samples of active carbon of $1150\;m^2/g$ surface area were impregnated with ammoniacal salts of copper, chromium and silver, with and without triethylenediamine. The samples of impregnated carbon were aged at $50^{\circ}C$, with and without 90% RH (relative humidity), for a little more than one year and chemically evaluated periodically. Initially copper (II) and chromium (VI) reduced very fast in the samples in humid atmosphere to the extent of 30% and 60% respectively in four months. These values were found to be unaffected by the presence of triethylenediamine (TEDA) indicating that the chemical did not retard the reduction process of chromium (VI) and copper (II). However, in the absence of humidity the reduction of the impregnants was significantly less (10-12%, w/w) in four months. It was quite evident; therefore, that the moisture was mainly responsible for the reduction of chromium (VI) and copper (II) species in impregnated carbons. The prolonged ageing of the samples with and without triethylenediamme after four months with and without humid atmosphere showed that the extent of reduction of chromium (VI) was very low, i.e. 5-10% and of copper (II) was 2-25%. Silver is not reduced due to carbon, as it remained unchanged in concentration on storage. The impregnated carbon samples (100 g) without triethylenediamine, which were aged at room temperature for 5 years in absence of humidity and unaged when evaluated against cyanogen chloride (CNCl) at a concentration of 4 mg/L and airflow rate of 30 lpm showed a high degree of protection (80- 110 minutes).

• Toxicological Research
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• v.29 no.1
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• pp.69-79
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• 2013

The purpose of this study was to investigate the current status of metal pollution in the sediment from rivers, lakes, and streams in active gold mining districts in Ghana. Two hundred and fifty surface sediment samples from 99 locations were collected and analyzed for concentrations of As, Hg, Cr, Co, Cu, Fe, Zn, Pb, Cd, Ni, and Mn using inductively coupled plasma-mass spectroscopy (ICP-MS). Metal concentrations were then used to assess the human health risks to resident children and adults in central tendency exposure (CTE) and reasonable maximum exposure (RME) scenarios. The concentrations of Pb, Cd, and As were almost twice the threshold values established by the Hong Kong Interim Sediment Quality Guidelines (ISQG). Hg, Cu, and Cr concentrations in sediment were 14, 20, and 26 times higher than the Canadian Freshwater Sediment Guidelines for these elements. Also, the concentrations of Pb, Cu, Cr, and Hg were 3, 11, 12, and 16 times more than the Australian and New Zealand Environment and Conservation Council (ANZECC) sediment guideline values. The results of the human health risk assessment indicate that for ingestion of sediment under the central tendency exposure (CTE) scenario, the cancer risks for child and adult residents from exposure to As were $4.18{\times}10^{-6}$ and $1.84{\times}10^{-7}$, respectively. This suggests that up to 4 children out of one million equally exposed children would contract cancer if exposed continuously to As over 70 years (the assumed lifetime). The hazard index for child residents following exposure to Cr(VI) in the RME scenario was 4.2. This is greater than the United States Environmental Protection Agency (USEPA) threshold of 1, indicating that adverse health effects to children from exposure to Cr(VI) are possible. This study demonstrates the urgent need to control industrial emissions and the severe heavy metal pollution in gold mining environments.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.29 no.12
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• pp.55-60
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• 2015

PG and RG methods are widely known method for calculating the capacity of the emergency generator in construction electrical installation. PG and RG methods are mainly used as a saving a life, fire protection, fire fighting in construction. Because no long distance between the emergency generator and electric motor feeder, the relatively small motor power in construction electrical installation, the capacity of generator in PG and RG methods are little problem of voltage and reactive power of generator. However in many cases the application of the PG and RG method is difficult in the Chemical Plant because it is long distance between the generator and the motor Feeder and motor capacity is very large. Motor starting power factor is about 0.2 lagging power factor and motor starting current is about 6times during motor staring. Also Most of the staring current component is a reactive power component. therefore, it is many cases that lack of reactive power and excess of allowable voltage drop limit and After selection of emergency diesel generator, problems happen during motor starting. Therefore, to be selection of effective emergency generator, active generator power, reactive power and the required reactive power during large motor starting should be considered in chemical plant. It is also required of the verification process through simulation because hand calculation is very difficult considering study cases.

• Journal of the Korea Institute of Building Construction
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• v.18 no.1
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• pp.59-65
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• 2018

Radon gas is a colorless, odorless, tasteless gas that occurs when uranium, a natural radioactive material in rocks and soils, collapses. 85% of the annual radiation exposure of the human body is due to natural radiation, of which 50% is radon. According to the US Environmental Protection Agency (EPA) survey, 62 out of 1,000 smokers and 7 out of 1,000 nonsmokers are exposed to lung cancer when exposed to radon gas for a long time. In order to reduce the risk of radon gas, activate carbon was used to fabricate matrix, and the pore properties and radon reduction properties were investigated. When the activate carbon was used, the radon gas concentration was drastically reduced and the graph was changed as the measurement period became longer. The pore distribution and microporous properties, which are one of the material properties of activate carbon, can be grasped.

• Korean Journal of Environmental Agriculture
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• v.34 no.1
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• pp.52-56
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• 2015

BACKGROUND: Three commercial biopesticides containing derris extract, which is permitted as a commercial biopesticide substances by the Environmentally-friendly Agriculture Promotion Act, have been marketed in Korea. But, the quantitative analytical method of active substances for crop protection in biopesticides containing derris extract has not known. METHODS AND RESULTS: Solid phase extraction (SPE) cartridge clean-up method for the quantitative analysis of rotenone and deguelin in biopesticides containing derris extract was developed and validated by ultra-performance liquid chromatography (UPLC). The clean-up method was established using hydrophilic lipophilic balance (HLB) SPE cartridges for the bioactive substances in biopesticides containing derris extract, and the eluate was analyzed to quantify the rotenone and deguelin by the UPLC. LOQ and recovery rates of rotenone and deguelin were 0.085 and 0.044 mg/L, 95.7 and 93.3%, respectively. The content of rotenone and deguelin in three biopesticides containing derris extract were analyzed by the developed method, the results showed 0.001-0.236 and

• Journal of Auto-vehicle Safety Association
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• v.10 no.4
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• pp.25-32
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• 2018

Due to the rapid increase in the number of vehicles, the physical and human losses caused by traffic accidents have become serious social problems. In the global trend, there have been active studies conducted on improving safety level of automobile in order to reduce the number of automobile accident. As a result of such research, traffic accidents continue to decline. In the case of South Korea, however, rate of death by automobile accident is 8.5 per 10,000 people and it is a seven rank among the countries in OECD (Organization for Economic Cooperation and Development). This average rate is almost double compared to average automobile accident rate per 10,000 vehicles, of other OECD countries in 2015. Consequently, many studies and policies currently have been conducted and made for increasing safety of pedestrians; however, they are only emphasizing characteristics of pedestrians and drivers. For this reason, this study suggests scenarios for establishment of test standard corresponding with domestic environment and international standard of AEB (Autonomous Emergency Braking) and conducts a real car test by scenarios by setting up a goal with a function for remaining distance after braking and then examine equation by comparing real car tests results and outcome after calculation. This is a theoretical method to predict a relative remaining distance after AEB prior to conducting a real car test for evaluation of safeness of automobile with AEB and it is expected that it solves problem of complication of real car test.